ab Mammalian Cell Lysis Buffer 5X Instructions for Use For mammalian cell lysis.
|
|
- Jocelin Webb
- 5 years ago
- Views:
Transcription
1 ab Mammalian Cell Lysis Buffer 5X Instructions for Use For mammalian cell lysis. This product is for research use only and is not intended for diagnostic use. Version: 1 Last Updated: 30 October 2013
2 1
3 Table of Contents Table of Contents 2 1. Overview 3 2. Protocol Summary 3 3. Kit Components 4 4. Storage and Stability 4 5. Materials Required, Not Supplied 4 6. Assay Protocol 5 7. Troubleshooting 6 2
4 1. Overview Abcam s Mammalian Cell Lysis Buffer 5X (ab179835) just requires a simple 5-fold dilution. 2. Protocol Summary Treat cells as desired, wash plate with PBS to remove residual media Lyse non-adherent cells with 1X Mammalian Cell lysis buffer to cell pellet Incubate at room temperature for min The cell lysate now is ready for further biochemical analysis 3
5 3. Kit Components Item Quantity Storage upon arrival Storage after use/ reconstitution Mammalian Cell Lysis Buffer 5X 10 ml 4 C 4 C 4. Storage and Stability Upon arrival, store at 4 C. Reagent is stable for at least 6 months if stored properly. 5. Materials Required, Not Supplied Petri dishes or well plates to grow cells Distilled water (ddh 2 O)or MilliQ PBS 4
6 6. Assay Protocol a) Warm up Mammalian Cell Lysis Buffer 5X to room temperature before use. b) Prepare 1X Mammalian Cell lysis buffer by adding 1 ml of Mammalian Cell Lysis Buffer 5X into 4 ml of MilliQ water, and mix them well. c) The 1X Mammalian Cell lysis buffer (from Step b) now is ready to use. d) Treat cells as desired, wash plate with PBS to remove residual media. e) The following table is the suggested volume for lysis of adherent cells. Plate 384-well 96-well 48-well 24-well 12-well 6-well 100cm 2 Volume 20 µl 50 µl 100 µl 150 µl 200 µl 300 µl 800 µl f) Lyse non-adherent cells by adding about 100 µl of 1X Mammalian Cell lysis buffer to 10 6 cell pellet. g) Incubate at room temperature for min. h) The cell lysate now is ready for further biochemical 5
7 7. Troubleshooting Problem Reason Solution Assay not working Unexpected results Assay buffer at wrong temperature Protocol step missed Plate read at incorrect wavelength Unsuitable microtiter plate for assay Measured at wrong wavelength Samples contain impeding substances Unsuitable sample type Sample readings are outside linear range Assay buffer must not be chilled - needs to be at RT Re-read and follow the protocol exactly Ensure you are using appropriate reader and filter settings (refer to datasheet) Fluorescence: Black plates (clear bottoms); Luminescence: White plates; Colorimetry: Clear plates. If critical, datasheet will indicate whether to use flat- or U-shaped wells Use appropriate reader and filter settings described in datasheet Troubleshoot and also consider deproteinizing samples Use recommended samples types as listed on the datasheet Concentrate/ dilute samples to be in linear range 6
8 Problem Reason Solution Samples with inconsistent readings Lower/ Higher readings in samples and standards Unsuitable sample type Samples prepared in the wrong buffer Samples not deproteinized (if indicated on datasheet) Cell/ tissue samples not sufficiently homogenized Too many freezethaw cycles Samples contain impeding substances Samples are too old or incorrectly stored Not fully thawed kit components Out-of-date kit or incorrectly stored reagents Reagents sitting for extended periods on ice Incorrect incubation time/ temperature Incorrect amounts used Refer to datasheet for details about incompatible samples Use the assay buffer provided (or refer to datasheet for instructions) Use the 10kDa spin column (ab93349) Increase sonication time/ number of strokes with the Dounce homogenizer Aliquot samples to reduce the number of freeze-thaw cycles Troubleshoot and also consider deproteinizing samples Use freshly made samples and store at recommended temperature until use Wait for components to thaw completely and gently mix prior use Always check expiry date and store kit components as recommended on the datasheet Try to prepare a fresh reaction mix prior to each use Refer to datasheet for recommended incubation time and/ or temperature Check pipette is calibrated correctly (always use smallest volume pipette that can pipette entire volume) 7
9 Problem Reason Solution Standard curve is not linear Not fully thawed kit components Pipetting errors when setting up the standard curve Incorrect pipetting when preparing the reaction mix Air bubbles in wells Concentration of standard stock incorrect Errors in standard curve calculations Use of other reagents than those provided with the kit Wait for components to thaw completely and gently mix prior use Try not to pipette too small volumes Always prepare a master mix Air bubbles will interfere with readings; try to avoid producing air bubbles and always remove bubbles prior to reading plates Recheck datasheet for recommended concentrations of standard stocks Refer to datasheet and re-check the calculations Use fresh components from the same kit 8
10 9
11 10
12 UK, EU and ROW Tel: +44-(0) Austria Tel: France Tel: Germany Tel: Spain Tel: Switzerland Tel (Deutsch): Tel (Français): US and Latin America Tel: ABCAM (22226) Canada Tel: China and Asia Pacific Tel: ( 中國聯通 ) Japan technical@abcam.co.jp Tel: +81-(0) Copyright 2013 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. 11 Copyright 2013 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print.
LDH-Cytotoxicity Assay Kit II
ab65393 LDH-Cytotoxicity Assay Kit II Instructions for Use For the rapid, sensitive and accurate measurement of LDH-Cytotoxicity in cell culture samples. This product is for research use only and is not
More informationTotal NAD and NADH Assay Kit (Colorimetric)
ab186032 Total NAD and NADH Assay Kit (Colorimetric) Instructions for Use An optimized assay for monitoring Total NAD and NADH. This product is for research use only and is not intended for diagnostic
More informationab Beta Galactosidase Detection Kit (Fluorometric) Instructions for Use For monitoring β-galactosidase activity in cells.
ab176721 Beta Galactosidase Detection Kit (Fluorometric) Instructions for Use For monitoring β-galactosidase activity in cells. This product is for research use only and is not intended for diagnostic
More informationFor the rapid, sensitive and accurate measurement of Tyrosine in biological samples.
ab185435 Tyrosine Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Tyrosine in biological samples. This product is for research use only and is not intended
More informationCell Viability Assay Kit (Bioluminescent)
ab65314 Cell Viability Assay Kit (Bioluminescent) Instructions for Use For the rapid, sensitive and accurate detection of apoptosis and cell proliferation in various samples. This product is for research
More informationADP/ATP Ratio Assay Kit (Bioluminescent)
ab65313 ADP/ATP Ratio Assay Kit (Bioluminescent) Instructions for Use For the rapid, sensitive and accurate measurement of the ratio of ADP/ATP in various samples. This product is for research use only
More informationFluo-8 No Wash Calcium Assay Kit
ab112129 Fluo-8 No Wash Calcium Assay Kit Instructions for Use For detecting calcium in cells by using our proprietary fluorescence probe This product is for research use only and is not intended for diagnostic
More informationAspartate Aminotransferase Activity Assay Kit (Fluorometric)
ab138878 Aspartate Aminotransferase Activity Assay Kit (Fluorometric) Instructions for Use For measurement of Aminotransferase activity (AST) in various biological samples. This product is for research
More informationFluo-8 Medium Removal Calcium Assay Kit
ab112128 Fluo-8 Medium Removal Calcium Assay Kit Instructions for Use For detecting calcium in cells by using our proprietary fluorescence probe This product is for research use only and is not intended
More informationRhod-4 No Wash Calcium Assay Kit
ab112157 Rhod-4 No Wash Calcium Assay Kit Instructions for Use For detecting calcium in cells without washing by using our proprietary fluorescence probe This product is for research use only and is not
More informationFor the rapid, sensitive and accurate measurement of Glutamate in various samples.
ab83389 Glutamate Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glutamate in various samples. This product is for research use only and is not intended for diagnostic
More informationFor the rapid, sensitive and accurate detection of MMP-1 inhibition by various compounds.
ab118973 MMP-1 Inhibitor Screening Kit Instructions for Use For the rapid, sensitive and accurate detection of MMP-1 inhibition by various compounds. This product is for research use only and is not intended
More informationFor the rapid, sensitive and accurate measurement of Lactate levels in various samples
ab65330 L Lactate Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Lactate levels in various samples This product is for research use only and is not intended
More informationPyrophosphate Assay Kit (Fluorometric)
ab112155 Pyrophosphate Assay Kit (Fluorometric) Instructions for Use For the measurement of Pyrophosphate using our proprietary fluorescence probe for screening inhibition or enzyme activity This product
More informationFor the rapid, sensitive and accurate measurement of Histone Acetyltransferase activity in cell and tissue lysates.
ab65352 Histone Acetyltransferase Activity Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Histone Acetyltransferase activity in cell and tissue lysates. This product
More informationNeutrophil Elastase Inhibitor Screening Kit
ab118971 Neutrophil Elastase Inhibitor Screening Kit Instructions for Use For the rapid, sensitive and accurate detection of Neutrophil Elastase inhibition in various samples. This product is for research
More informationXanthine Oxidase Assay Kit
ab102522 Xanthine Oxidase Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Xanthine Oxidase activity in various samples. This product is for research use only and is
More informationFor the rapid, sensitive and accurate measurement of Ferrous and/or Ferric ions in various samples.
ab83366 Iron Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Ferrous and/or Ferric ions in various samples. This product is for research use only and is not intended
More informationNitric Oxide Assay Kit (Fluorometric)
ab65327 Nitric Oxide Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Nitric Oxide in various samples This product is for research use only and is not
More informationUniversal Kinase Assay Kit (Fluorometric) For monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity
ab138879 Universal Kinase Assay Kit (Fluorometric) Instructions for Use For monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity This product is for research use
More informationCell Viability Assay Kit Fluorometric Near InfraRed
ab112123 Cell Viability Assay Kit Fluorometric Near InfraRed Instructions for Use For detecting cell viability in suspension and adherent cells by using Near InfraRed fluorescence probes This product is
More informationFor the rapid, sensitive and accurate measurement of Creatinine in various samples.
ab65340 Creatinine Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Creatinine in various samples. This product is for research use only and is not intended for diagnostic
More informationGlucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
ab102529 Glucose 6 Phosphate Dehydrogenase Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose 6 Phosphate Dehydrogenase activity in various samples This product
More informationAlanine Transaminase Activity Assay Kit
ab105134 Alanine Transaminase Activity Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Alanine Transaminase activity in various samples. This product is for research
More informationADP/ATP Ratio Assay Kit (Bioluminescent)
ab65313 ADP/ATP Ratio Assay Kit (Bioluminescent) Instructions for Use For the rapid, sensitive and accurate measurement of the ratio of ADP/ATP in various samples. This product is for research use only
More informationCell Viability Assay Kit Fluorometric Blue
ab112120 Cell Viability Assay Kit Fluorometric Blue Instructions for Use For detecting cell viability in suspension and adherent cells by using our proprietary Blue fluorescence probe This product is for
More informationLysyl Oxidase Activity Assay Kit - Red Fluorescence
ab112139 Lysyl Oxidase Activity Assay Kit - Red Fluorescence Instructions for Use For detecting Lysyl Oxidase Activity in physiology solutions by using our proprietary red fluorescence probe This product
More informationFor the rapid, sensitive and accurate measurement of Citrate levels in various samples
ab83396 Citrate Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Citrate levels in various samples This product is for research use only and is not intended for diagnostic
More informationMMP Activity Assay Kit (Fluorometric - Red)
ab112147 MMP Activity Assay Kit (Fluorometric - Red) Instructions for Use For detecting MMP activity in biological samples using our proprietary red fluorescence probe This product is for research use
More informationab Generic Caspase Activity Assay Kit Fluorometric Green
ab112130 Generic Caspase Activity Assay Kit Fluorometric Green Instructions for Use For detecting Caspase activity in cells by using our proprietary green fluorescence probe. This product is for research
More informationab Proteasome 20S Activity Assay Kit (Fluorometric)
ab112154 Proteasome 20S Activity Assay Kit (Fluorometric) Instructions for Use For detecting proteasome activity in cultured cells using our proprietary green fluorescence probe. This product is for research
More informationab Nuclear (Nucleic Acid-Free) Extraction Kit
ab113477 Nuclear (Nucleic Acid-Free) Extraction Kit Instructions for Use For the preparation of nuclear (nucleic acid-free) extracts from mammalian cells and tissue samples. This product is for research
More informationProtein Quantitation Kit (Bradford Assay)
ab102535 Protein Quantitation Kit (Bradford Assay) Instructions for Use For the rapid, sensitive and accurate measurement of Protein in various samples This product is for research use only and is not
More informationFor the rapid, sensitive and accurate measurement of Caspase 3 Activity in cell and tissue lysates.
ab39401 Caspase 3 Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Caspase 3 Activity in cell and tissue lysates. This product is for research use only
More informationab Fluo-8 No Wash Calcium Assay Kit
ab112129 Fluo-8 No Wash Calcium Assay Kit Instructions for Use For detecting calcium in cells by using our proprietary fluorescence probe. This product is for research use only and is not intended for
More informationab Thrombin Inhibitor Screening Assay Kit (Fluorometric)
ab197007 Thrombin Inhibitor Screening Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate screening of potential inhibitors of Thrombin. This product is for research use
More informationFor the rapid, sensitive and accurate measurement of apoptosis in various samples.
ab102499 Caspase Cascade Screening Kit Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and is not intended
More informationab MDR Assay Kit (Fluorometric)
ab112142 MDR Assay Kit (Fluorometric) Instructions for Use For detecting MDR pump activities in cells using our proprietary fluorescence probe. This product is for research use only and is not intended
More informationab Nuclear Extraction Kit
ab113474 Nuclear Extraction Kit Instructions for Use For the preparation of nuclear extracts from mammalian cells and tissue samples View kit datasheet: www.abcam.com/ab113474 (use www.abcam.cn/ab113474
More informationab Plasmin Activity Assay Kit (Fluorometric)
ab204728 Plasmin Activity Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate detection of Plasmin activity. This product is for research use only and is not intended for diagnostic
More informationab Tyrosinase Inhibitor Screening Assay Kit (Colorimetric)
ab204715 Tyrosinase Inhibitor Screening Assay Kit (Colorimetric) Instructions for Use For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors. This product is for research use only
More informationab Plasmin Inhibitor Screening Assay Kit (Fluorometric)
ab204729 Plasmin Inhibitor Screening Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate screening of potential Plasmin inhibitors. This product is for research use only and
More informationab MTS Cell Proliferation Assay Kit (Colorimetric)
ab197010 MTS Cell Proliferation Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of cell proliferation. View kit datasheet: www.abcam.com/ab197010 (use www.abcam.cn/ab197010
More informationab Adenosylhomocysteinase (AHCY) Inhibitor Screening Assay Kit (Fluorometric)
+ ab204694 Adenosylhomocysteinase (AHCY) Inhibitor Screening Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate screening of potential Adenosylhomocysteinase (AHCY) inhibitors.
More informationpan-caspase (Active) Red Staining Kit
ab65616 pan-caspase (Active) Red Staining Kit Instructions for Use For the rapid, sensitive and accurate detection of activated Caspases in living cells. This product is for research use only and is not
More informationab65327 Nitric Oxide Assay Kit (Fluorometric)
ab65327 Nitric Oxide Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of nitric oxide in various samples. This product is for research use only and is not
More informationab Cell Viability Assay Kit Fluorometric Dual Green/Red
ab112121 Cell Viability Assay Kit Fluorometric Dual Green/Red Instructions for Use For detecting cell viability in suspension and adherent cells by using dual proprietary green and red fluorescence probes.
More informationab CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 405 nm)
ab176739 - CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 405 nm) Instructions for Use For evaluation of the viability of mammalian cells by fluorescent labeling and flow cytometry.
More informationab CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm)
ab176738 - CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) Instructions for Use For evaluation of the viability of mammalian cells by fluorescent labeling and flow cytometry.
More informationab Factor IXa Activity Assay Kit (Fluorometric)
ab204727 Factor IXa Activity Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate detection of Factor IXa activity. This product is for research use only and is not intended
More informationEnhanced Apoptotic DNA Ladder Detection Kit
ab66093 Enhanced Apoptotic DNA Ladder Detection Kit Instructions for Use For the rapid, sensitive and accurate detection of DNA fragmentation in apoptotic cells and tissues This product is for research
More informationab DNA Isolation Kit - Plasma/Serum
ab156893 DNA Isolation Kit - Plasma/Serum Instructions for Use For the Isolation of small amounts of DNA from various samples. This product is for research use only and is not intended for diagnostic use.
More informationab Factor Xa Inhibitor Screening Assay Kit (Fluorometric)
ab204712 Factor Xa Inhibitor Screening Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate screening of potential Factor Xa inhibitors. This product is for research use only
More informationab Histone Extraction Kit
ab113476 Histone Extraction Kit Instructions for Use For the extraction of histone proteins from mammalian cells and tissue This product is for research use only and is not intended for diagnostic use.
More informationab Glucose Uptake Assay Kit (Cell-based)
ab204702 Glucose Uptake Assay Kit (Cell-based) Instructions for Use For measuring glucose uptake via flow cytometry and fluorescent microscopy. This product is for research use only and is not intended
More informationab65354 Superoxide Dismutase Activity Assay kit (Colorimetric)
ab65354 Superoxide Dismutase Activity Assay kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Superoxide Dismutase Activity in various samples. This product is
More informationab JC-10 Mitochondrial Membrane Potential Assay Kit Flow Cytometry
ab112133 JC-10 Mitochondrial Membrane Potential Assay Kit Flow Instructions for Use For detecting mitochondrial membrane potential changes in cells using our proprietary fluorescence probe. This product
More informationab39412 Caspase 1 Assay Kit (Fluorometric)
ab39412 Caspase 1 Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate measurement of Caspase 1 activity in cell and tissue lysates. This product is for research use only and
More informationTissue Factor Human Chromogenic Activity Assay Kit
ab108906 Tissue Factor Human Chromogenic Activity Assay Kit Instructions for Use For the quantitative measurement of Human Tissue Factor activity in plasma, serum, urine, tissue and cell culture extracts
More informationAvidin/Biotin Blocking Kit
Version 1 Last updated 25 June 2018 ab64212 Endogenous Avidin/Biotin Blocking Kit For the blocking of endogenous biotin during immunohistochemistry. This product is for research use only and is not intended
More informationab PCR Clean-up Kit
ab156894 PCR Clean-up Kit Instructions for Use For efficient clean-up of PCR products from various sources. This product is for research use only and is not intended for diagnostic use. Version 2 Last
More informationab Factor VIIIa Activity Assay Kit (Fluorometric)
ab204696 Factor VIIIa Activity Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate detection of Factor VIII activity. This product is for research use only and is not intended
More informationab Nile Red Staining Kit 1
Version 1 Last updated 15 March 2018 ab228553 Nile Red Staining Kit For the measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader.
More informationab BSA Removal Kit
ab173231 BSA Removal Kit Instructions for Use For the removal of BSA from antibodies prior to conjugation This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated
More informationab Calcium Detection Assay kit (Colorimetric)
ab102505 Calcium Detection Assay kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Calcium concentration in various samples. This product is for research use
More informationUniversal Methyltransferase Activity Assay Kit
ab139433 Universal Methyltransferase Activity Assay Kit Instructions for Use A complete kit for the screening of candidate compounds that may alter normal Methyltransferase activity. This product is for
More informationab Fura-2 No Wash Calcium Assay Kit
ab176766 Fura-2 No Wash Calcium Assay Kit Instructions for use: For the measurement of intracellular calcium mobilization in live cells. This product is for research use only and is not intended for diagnostic
More informationab Glutamine Assay Kit (Colorimetric)
ab197011 Glutamine Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Glutamine in various biological samples. This product is for research use only and
More informationab CytoPainter Mitochondrial Staining Kit NIR Fluorescence
ab176747 CytoPainter Mitochondrial Staining Kit NIR Fluorescence Instructions for Use For staining Mitochondria in live cells with our proprietary NIR probe. This product is for research use only and is
More informationab Plant Chromatin Extraction Kit
ab156906 Plant Chromatin Extraction Kit Instructions for Use For isolating chromatin or DNA-protein complex from plants in a simple and rapid format This product is for research use only and is not intended
More informationab ATPase Assay Kit (Colorimetric)
Version 1 Last updated 25 April 2018 ab234055 ATPase Assay Kit (Colorimetric) For the measurement of ATPase activity in various tissues/cells. This product is for research use only and is not intended
More informationab Enterokinase cleavage kit
ab207001 Enterokinase cleavage kit Instructions for use: For efficiently removing tags from recombinant fusion proteins containing accessible enteropeptidase-specific recognition sequence. This product
More informationab83371 Alkaline Phosphatase Assay Kit (Fluorometric)
ab83371 Alkaline Phosphatase Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of alkaline phosphatase in various samples. View kit datasheet: www.abcam.com/ab83371
More informationab Complex I Rodent Protein Quantity Dipstick Assay Kit
ab109875 Complex I Rodent Protein Quantity Dipstick Assay Kit Instructions for Use For the quantitative measurement of Complex I Protein Quantity in samples from Mouse and Rat This product is for research
More informationab DNA Isolation Kit - Urine
ab156899 DNA Isolation Kit - Urine Instructions for Use For quick and efficient isolation of DNA from urine. This product is for research use only and is not intended for diagnostic use. Version 1 Last
More informationab Thrombin Activity Assay Kit (Fluorometric)
ab197006 Thrombin Activity Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Thrombin activity in plasma. This product is for research use only and is not
More informationab DNA-Protein Binding Assay Kit (Colorimetric)
ab117139 DNA-Protein Binding Assay Kit (Colorimetric) Instructions for Use For the measurement protein-dna interactions in vitro, specifically for detecting transcription factor activation using mammalian
More informationab Urine m6a Assay Kit
Version 1 Last updated 3 April 2018 ab233493 Urine m6a Assay Kit For detecting total urinary m 6 A levels, resulting from whole body turnover or degradation of DNA/RNA containing m 6 A, using urine from
More informationab BSA Removal Kit
ab173231 BSA Removal Kit Instructions for Use For the removal of BSA from antibodies prior to conjugation. Please note that this kit is not compatible with our range of GOLD conjugation kits. To remove
More informationab DNA-Protein Binding Assay Kit (Fluorometric)
ab117140 DNA-Protein Binding Assay Kit (Fluorometric) Instructions for Use For the measurement protein-dna interactions in vitro, specifically for detecting transcription factor activation using mammalian
More informationab83355 ATP Assay Kit (Colorimetric/ Fluorometric)
Version 18 Last updated 2 January 2018 ab83355 ATP Assay Kit (Colorimetric/ Fluorometric) For the rapid, sensitive and accurate measurement of ATP in a variety of samples. This product is for research
More informationab ADHP HRP substrate
ab205810 ADHP HRP substrate Instructions for Use In the presence of H2O2, HRP converts ADHP to the fluorogenic product, resorufin. This product is for research use only and is not intended for diagnostic
More informationab Histone H4 Acetylation Assay Kit
ab115103 Histone H4 Acetylation Assay Kit Instructions for Use For the measurement of global histone H4 acetylation using a variety of mammalian cells, including Tissue, adherent and suspension cells This
More informationCytoPainter Cell Proliferation Staining Reagent Blue Fluorescence (Ex-405nm)
ab176726 CytoPainter Cell Proliferation Staining Reagent Blue Fluorescence (Ex-405nm) Instructions for Use For fluorescent labeling and analysis on heterogeneous cell populations. This product is for research
More informationab Histone H2B Acetyl (K5) ELISA Kit
ab138916 Histone H2B Acetyl (K5) ELISA Kit Instructions for Use For the quantitative measurement of acetylated lysine (K5) on Histone H2B in cell lysates. This product is for research use only and is not
More informationab Histone H3 Acetylation Assay Kit
ab115102 Histone H3 Acetylation Assay Kit Instructions for Use For the measurement of global histone H3 acetylation using a variety of mammalian cells This product is for research use only and is not intended
More informationab Bacterial Assay (Luminescent)
Version 1 Last updated 29 May 2018 ab189820 Bacterial Assay (Luminescent) For the measurement of bacterial cells in a number of media or biological sources. This product is for research use only and is
More informationab Rabbit specific IHC polymer detection kit HRP/DAB
ab209101 Rabbit specific IHC polymer detection kit HRP/DAB Instructions for use: For detection of rabbit antibodies bound to antigens in tissue sections using light microscopy. This product is for research
More informationab DNA Isolation and Bisulfite Conversion Kit
ab117124 DNA Isolation and Bisulfite Conversion Kit Instructions for Use For DNA isolation and bisulfite conversion This product is for research use only and is not intended for diagnostic use. Version
More informationFor labelling sub-cellular organelles in tissue sections, cell cultures and cell free experiments using our proprietary Red fluorescence probe
ab112127 CytoPainter F-actin Staining Kit - Red Fluorescence Instructions for Use For labelling sub-cellular organelles in tissue sections, cell cultures and cell free experiments using our proprietary
More informationab Complex IV Rodent Enzyme Activity Microplate Assay Kit
ab109911 Complex IV Rodent Enzyme Activity Microplate Assay Kit Instructions for use: To determine the activity of Cytochrome C Oxidase in Mouse and Rat samples This product is for research use only and
More informationab Biotin (Type A) Fast Conjugation Kit
ab201795 Biotin (Type A) Fast Conjugation Kit Instructions for Use For the Conjugation of Antibodies or Proteins to Biotin (Type A). This product is for research use only and is not intended for diagnostic
More informationab FAP Human ELISA Kit
ab193701 FAP Human ELISA Kit Instructions for Use For the quantitative measurement of human Fibroblast Activation Protein, alpha (FAP) in serum, plasma, and cell culture supernatants. This product is for
More informationab Soluble Collagen Assay Kit
Version 1 Last updated 6 December 2018 ab242291 Soluble Collagen Assay Kit View Soluble Collagen Assay Kit datasheet: www.abcam.com/ab242291 For the detection of soluble collagen from cell or tissue sample.
More informationab Immunoprecipitation kit
ab206996 Immunoprecipitation kit Instructions for use: For efficient immunoprecipitation (IP) and coimmunoprecipitation (Co-IP). This product is for research use only and is not intended for diagnostic
More informationab Biotin (Type B) Fast Conjugation Kit
ab201796 Biotin (Type B) Fast Conjugation Kit Instructions for Use For the Conjugation of Antibodies or Proteins to Biotin (Type B). This product is for research use only and is not intended for diagnostic
More informationLive and Dead Cell Assay
ab115347 Live and Dead Cell Assay Instructions for Use Differential fluorescent labeling of live and dead cells This product is for research use only and is not intended for diagnostic use. Last Updated
More informationab Biotin (Type A) Fast Conjugation Kit
ab201795 Biotin (Type A) Fast Conjugation Kit Instructions for Use For the Conjugation of Antibodies or Proteins to Biotin (Type A). This product is for research use only and is not intended for diagnostic
More information