The Control of Microbial Growth

Size: px
Start display at page:

Download "The Control of Microbial Growth"

Transcription

1 The Control of Microbial Growth Sepsis refers to microbial contamination. Asepsis is the absence of significant contamination. Aseptic surgery techniques prevent microbial contamination of wounds. Terminology Sterilization: Removal of all microbial life Commercial Sterilization: Killing C. botulinum endospores Disinfection: Removal of pathogens Antisepsis: Removal of pathogens from living tissue Degerming: Removal of microbes from a limited area Sanitization: Lower microbial counts on eating utensils Biocide/Germicide: Kills microbes Bacteriostasis: Inhibiting, not killing, microbes Decimal Reduction or D Value Bacterial populations die at a constant logarithmic rate. Figure 7.1a 1

2 Situational Considerations Daily Life Hospitals Microbiology Laboratories Food and Food Production Facilities Water Treatment Facilities Other Industries Effectiveness of antimicrobial treatment depends on: Number of microbes Environment (organic matter, temperature, biofilms) Time of exposure Type of microorganism Figure 7.1b Actions of Microbial Control Agents Alteration of membrane permeability Damage to proteins Damage to nucleic acids 2

3 Heat Moist heat denatures proteins Autoclave: Steam under pressure Figure 7.2 Physical Methods of Microbial Control Pasteurization reduces spoilage organisms and pathogens Equivalent treatments 63 C for 30 min High-temperature short-time 72 C for 15 sec Ultra-high-temperature: 140 C for <1 sec Thermoduric organisms survive 3

4 Physical Methods of Microbial Control Dry Heat Sterilization kills by oxidation Flaming Incineration Hot-air sterilization Hot-air Autoclave Equivalent treatments 200 C, 90 minutes 121 C, 15 min Physical Methods of Microbial Control Filtration removes microbes Low temperature inhibits microbial growth Refrigeration Deep freezing High pressure denatures proteins Desiccation prevents metabolism Lyophilization or freeze-drying Osmotic pressure causes plasmolysis Use salts and sugars to create a hypertonic environment Physical Methods of Microbial Control Radiation damages DNA Ionizing radiation (X rays, gamma rays, electron beams) Nonionizing radiation (UV) (Microwaves kill by heat; not especially antimicrobial) 4

5 Figure 7.5 Chemical Methods of Microbial Control Principles of effective disinfection Concentration of disinfectant Organic matter ph Time Chemical Methods of Microbial Control Evaluating a disinfectant Disk-diffusion method Figure 7.6 5

6 Phenol Phenolics. Lysol Bisphenols. Hexachlorophene, Triclosan Disrupt plasma membranes Figure 7.7 Halogens. Iodine, Chlorine Oxidizing agents Bleach is hypochlorous acid (HOCl) Alcohols. Ethanol, isopropanol Denature proteins, dissolve lipids Table 7.6 6

7 Surface-Active Agents or Surfactants Soap Acid-anionic detergents Quarternary ammonium compounds Cationic detergents Degerming Sanitizing Bactericidal, Denature proteins, disrupt plasma membrane Chemical Food Preservatives Organic Acids Inhibit metabolism Sorbic acid, benzoic acid, calcium propionate Control molds and bacteria in foods and cosmetics Nitrite prevents endospore germination Antibiotics. Nisin and natamycin prevent spoilage of cheese Aldehydes Inactivate proteins by cross-linking with functional groups ( NH 2, OH, COOH, SH) Glutaraldehyde, formaldehyde Gaseous Sterilants Denature proteins Ethylene oxide Peroxygens Oxidizing agents O 3, H 2 O 2 7

8 Microbial Characteristics and Microbial Control Figure

The Control of Microbial Growth

The Control of Microbial Growth The Control of Microbial Growth Sepsis refers to microbial contamination. Asepsis is the absence of significant contamination. Aseptic surgery techniques prevent microbial contamination of wounds. Terminology

More information

M I C R O B I O L O G Y

M I C R O B I O L O G Y ninth edition TORTORA FUNKE CASE M I C R O B I O L O G Y a n i n t r o d u c t i o n 7 The Control of Microbial Growth PowerPoint Lecture Slide Presentation prepared by Christine L. Case The Control of

More information

Chapter 7. The Control of Microbial Growth

Chapter 7. The Control of Microbial Growth Chapter 7 The Control of Microbial Growth The Terminology of Microbial Control Sepsis refers to microbial contamination / microbial growth Asepsis is the absence of significant contamination Antisepsis:

More information

Controlling Microbial Growth

Controlling Microbial Growth Controlling Microbial Growth What factors limit microbial growth? In what situations are large microbial numbers undesirable? Concept of Microbial Control Factors Which Affect Control Temp., species type

More information

The Control of Microbial Growth

The Control of Microbial Growth PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College C H A P T E R 7 The Control of Microbial Growth The Terminology of Microbial Control Sepsis refers to bacterial

More information

Chapter 7 Study Guide Control of Microbial Growth

Chapter 7 Study Guide Control of Microbial Growth Chapter 7 Study Guide Control of Microbial Growth Note: you will not be tested on the following: use-dilution test. 1. Be able to define and use the following terms in context: sterilization, commercial

More information

The Control of Microbial Growth

The Control of Microbial Growth 11/10/2016 PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College CHAPTER 7 The Control of Microbial Growth The Terminology of Microbial Control Sepsis refers to

More information

Chapter 7: Control of Microbial Growth

Chapter 7: Control of Microbial Growth Chapter 7: Control of Microbial Growth 1. Physical Methods 2. Chemical methods Important Terminology sterilization > commercial sterilization > disinfection = antisepsis > degerming > sanitization Also,

More information

Important Terminology

Important Terminology Chapter 7: Control of Microbial Growth 1. Physical Methods 2. Chemical methods Important Terminology sterilization > commercial sterilization > disinfection = antisepsis > degerming > sanitization Also,

More information

Important Terminology (pg )

Important Terminology (pg ) Number of living microbes 10/18/2016 Chapter 9: Control of Microbial Growth 1. Physical Methods 2. Chemical methods Important Terminology (pg. 263-264) sterilization > commercial sterilization > disinfection

More information

Control of Microbial growth Dr. Hala Al Daghistani

Control of Microbial growth Dr. Hala Al Daghistani Control of Microbial growth Dr. Hala Al Daghistani Terminology Sepsis: Characterized by the presence of pathogenic microbes in living tissues or associated fluids. Asepsis: absence of significant contamination.

More information

Foundations in Microbiology Seventh Edition. Talaro Chapter 11 Physical and Chemical Agents for Microbial Control

Foundations in Microbiology Seventh Edition. Talaro Chapter 11 Physical and Chemical Agents for Microbial Control Foundations in Microbiology Seventh Edition Talaro Chapter 11 Physical and Chemical Agents for Microbial Control 11.1 Controlling Microorganisms Physical, chemical, and mechanical methods to destroy or

More information

Control of Microbial growth Dr. Hala Al Daghistani

Control of Microbial growth Dr. Hala Al Daghistani Control of Microbial growth Dr. Hala Al Daghistani Terminology Sepsis: Characterized by the presence of pathogenic microbes in living tissues or associated fluids. Asepsis: absence of significant contamination.

More information

Definitions. BIOL 3702: Chapter 8. Control of Microbes in the Environment. Mechanical Removal Methods. Pattern of Microbial Death

Definitions. BIOL 3702: Chapter 8. Control of Microbes in the Environment. Mechanical Removal Methods. Pattern of Microbial Death Definitions Control of Microbes in the Environment u Sterilization - destruction or removal of all viable organisms from an object or environment (agent = sterilant) u Disinfection - killing, inhibition,

More information

Chapter 9. Controlling Microbial Growth in the Environment. Lecture prepared by M indy M iller-kittrell North Carolina State University

Chapter 9. Controlling Microbial Growth in the Environment. Lecture prepared by M indy M iller-kittrell North Carolina State University Chapter 9 Controlling Microbial Growth in the Environment Lecture prepared by M indy M iller-kittrell North Carolina State University Table 9.1 Terminology of Microbial Control Figure 9.1 A plot of microbial

More information

Definitions. BIOL 3702: Chapter 8. Control of Microbes in the Environment. Mechanical Removal Methods. Pattern of Microbial Death

Definitions. BIOL 3702: Chapter 8. Control of Microbes in the Environment. Mechanical Removal Methods. Pattern of Microbial Death Definitions Control of Microbes in the Environment u Sterilization - destruction or removal of all viable organisms from an object or environment (agent = sterilant) u Disinfection - killing, inhibition,

More information

Control of microbial growth means "Preventing the growth of microbes. Preventing growth of undesirable microorganisms

Control of microbial growth means Preventing the growth of microbes. Preventing growth of undesirable microorganisms Control of microbial growth means "Preventing the growth of microbes OR Preventing growth of undesirable microorganisms Very important in microbiology experiments Control Killing microorganisms Preventing

More information

Chapter 9 Controlling Microbial Growth in the Environment.

Chapter 9 Controlling Microbial Growth in the Environment. Chapter 9 Controlling Microbial Growth in the Environment http://www.cdc.gov/hicpac/disinfection_sterilization/acknowledg.html Controlling microorganisms Decontamination: Physical, chemical, and mechanical

More information

Physical and Chemical Control of Microorganisms

Physical and Chemical Control of Microorganisms 1 Physical and Chemical Control of Microorganisms I. Terms II. Factors which determine the effectiveness of control methods III. Methods of physical control IV. Chemical agents Terms 1) Control -- Limiting

More information

8. Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is: A. disinfection B. sterilization C. antisepsis D.

8. Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is: A. disinfection B. sterilization C. antisepsis D. 11 Student: 1. Microbiological contaminants are best described as: A. unwanted microbes present on or in a substance B. any and all microbes present on or in a substance C. pathogenic microbes present

More information

Physical and Chemical Control of Microbes. Muhammad Suleman Kamran Rasool Fatima Amjad Aysha Imtiaz BIOL 411

Physical and Chemical Control of Microbes. Muhammad Suleman Kamran Rasool Fatima Amjad Aysha Imtiaz BIOL 411 BIOL 411 Physical and Chemical Control of Microbes Muhammad Suleman Kamran Rasool Fatima Amjad Aysha Imtiaz BIOL 411 Background: Physical and Chemical Control of Microbial Growth In the 19 th century,

More information

AUTOCLAVE: steam pressure sterilizer

AUTOCLAVE: steam pressure sterilizer Microbiology Chapter 6 Controlling Microbes and Antimicrobial Agents 6:1 Physical Methods for Controlling Microbes DISINFECTION: the process of destroying disease-causing microorganisms STERILIZATION:

More information

Inhibiting Microbial Growth in vitro. CLS 212: Medical Microbiology Zeina Alkudmani

Inhibiting Microbial Growth in vitro. CLS 212: Medical Microbiology Zeina Alkudmani Inhibiting Microbial Growth in vitro CLS 212: Medical Microbiology Zeina Alkudmani Microbicidal or Microbistatic? Microbicidal Microbicidal is the process or an agent that kills the microorganism. The

More information

Chapter 9 Controlling Microbial Growth in the Environment. 10/1/ MDufilho

Chapter 9 Controlling Microbial Growth in the Environment. 10/1/ MDufilho Chapter 9 Controlling Microbial Growth in the Environment 10/1/2017 1 MDufilho Table 91 Terminology of Microbial Control 10/1/2017 MDufilho 2 Number of living microbes Figure 91 A plot of microbial death

More information

2054, Chap. 7, page 1

2054, Chap. 7, page 1 2054, Chap. 7, page 1 I. Control of Microorganisms by Physical and Chemical Methods A. Terminology 1. sterilization = destruction of living cells, viable spores, viruses, viroids 2. disinfection = killing,

More information

Chapter 11. Topics: Controlling Microorganisms. - Physical Control. - Chemical control

Chapter 11. Topics: Controlling Microorganisms. - Physical Control. - Chemical control Chapter 11 Topics: Controlling Microorganisms - Physical Control - Chemical control 1 An overview of the microbial control methods. Fig. 11.1 Microbial control methods 2 Controlling Microorganisms Microbial

More information

Inhibiting of Microbial Growth in vitro CLS 212

Inhibiting of Microbial Growth in vitro CLS 212 Inhibiting of Microbial Growth in vitro CLS 212 Microbicidal Microbicidal is the process or an agent that kills the microorganism. The suffix -cidal or cide means??( See chapter 8 page 131) Microbistatic

More information

Physical and Chemical Agents for Microbial Control

Physical and Chemical Agents for Microbial Control Chapter 11 Physical and Chemical Agents for Microbial Control Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Controlling Microorganisms Physical, chemical, and

More information

Chapter 8 Control of Microorganisms by Physical and Chemical Agents

Chapter 8 Control of Microorganisms by Physical and Chemical Agents Chapter 8 Control of Microorganisms by Physical and Chemical Agents Why control the microbial activity? Prevention from : Food spoilage and Contamination Pathogen and their transmission Longer preservation

More information

1. # of organisms present- it is harder to kill a larger population of cells.

1. # of organisms present- it is harder to kill a larger population of cells. ANTIMICROBIAL AGENTS Killing of Organisms-Death depends upon 1. # of organisms present- it is harder to kill a larger population of cells. 2. # of molecules (bullets of chemical agents)- a more concentrated

More information

Sterilization and Disinfection

Sterilization and Disinfection BACTERIOLOGY MSc. Halah Dawood Second stage LAB. 1 Sterilization and Disinfection Sterilization is defined as the process where all the living microorganisms, including bacterial spores are killed. Sterilization

More information

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Environment PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 9 Controlling Microbial Growth in the Environment Bacteristatic vs cidal vs sterilize?

More information

Microbial Growth and Aseptic Techniques

Microbial Growth and Aseptic Techniques Microbial Growth and Aseptic Techniques Control of Microbial Growth: Introduction Early civilizations practiced salting, smoking, pickling, drying, and exposure of food and clothing to sunlight to control

More information

Controlling Microbes (Sterilization & Disinfection)

Controlling Microbes (Sterilization & Disinfection) Controlling Microbes (Sterilization & Disinfection) Some Important Terms Defined: Sterilization - treatment to destroy all microbial life (even destroys bacterial endospores and fungal spores); there are

More information

Principles of Microbial Control Terminology of Microbial Control Death Rate Action of Anti-microbial agents

Principles of Microbial Control Terminology of Microbial Control Death Rate Action of Anti-microbial agents Principles of Microbial Control Terminology of Microbial Control Death Rate Action of Anti-microbial agents Selection of Anti-microbial agents Factors affecting efficacy BioSafety Levels Methods of Microbial

More information

Microorganisms are the agents of contamination, infection. Hence it becomes necessary to remove them from materials and areas.

Microorganisms are the agents of contamination, infection. Hence it becomes necessary to remove them from materials and areas. Mawada M.yahia Objectives 1.Define the terms sterilization, disinfectant and antiseptic. 2. Classify the different methods of sterilization 3. Rrealizes that heat is the most important method of sterilization.

More information

on ro ero la THE TERMINOLOGY OF MICROBIAL CONTROL THE RATE OF MICROBIAL DEATH ACTIONS OF MICROBIAL CONTROL AGENTS

on ro ero la THE TERMINOLOGY OF MICROBIAL CONTROL THE RATE OF MICROBIAL DEATH ACTIONS OF MICROBIAL CONTROL AGENTS e on ro ero la THE TERMINOLOGY OF MICROBIAL CONTROL Sterilization is the removal or destruction of all forms of microbial life (although it usually assumes the absence of prions which are rare but exceptionally

More information

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Environment PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 9 Controlling Microbial Growth in the Environment Principles of Microbial Control Terminology

More information

Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7)

Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7) Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus Primary Source for figures and content:

More information

Chapter 9: Controlling Microbial Growth in the Environment

Chapter 9: Controlling Microbial Growth in the Environment Chapter 9: Controlling Microbial Growth in the Environment Control of Microbial Growth: Introduction Early civilizations practiced salting, smoking, pickling, drying, and exposure of food and clothing

More information

How antimicrobial agents work

How antimicrobial agents work Physical and Chemical Control of Microbes Physical Agents heat or radiation Chemical Agents disinfectants or antiseptics Important Terms 1. Sterilization process of killing all viable microbes 2. Bactericide

More information

Sterilization, Disinfection and Antisepsis

Sterilization, Disinfection and Antisepsis Sterilization, Disinfection and Antisepsis For the control of infections Prevention Principles of Sterilization, Disinfection and Antisepsis Treatment Chemotherapy Definitions Sterilization Disinfection

More information

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Environment PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 9 Controlling Microbial Growth in the Environment Why do Gram Staining? Classification/identification

More information

1)What are the four general considerations for effective microbial control? List and describe (8 pts)

1)What are the four general considerations for effective microbial control? List and describe (8 pts) ä.2 Name: Sanitation work sheet Answer Key Not to be turned in... 1)What are the four general considerations for effective microbial control? List and describe (8 pts) 1) Sterilization: complete removal

More information

Claire Kari Biosafety Specialist DEHS Biosafety October 2010

Claire Kari Biosafety Specialist DEHS Biosafety October 2010 Claire Kari Biosafety Specialist DEHS Biosafety 612 626 6002 karix001@umn.edu October 2010 Welcome to DEHS introductory training about Decontamination Decontamination Definitions Categories Prep Work Methods

More information

Lecture Summary Microbial Control of Growth (CH5)

Lecture Summary Microbial Control of Growth (CH5) Lecture Summary Microbial Control of Growth (CH5) This chapter covers the processes available to control microbial growth. These processes are divided into two groups, physical and chemical methods of

More information

مادة االدوية املرحلة الثالثة أ.م.د. حسام الدين سامل

مادة االدوية املرحلة الثالثة أ.م.د. حسام الدين سامل مادة االدوية املرحلة الثالثة أ.م.د. حسام الدين سامل 2017-2016 ANTISEPTICS AND DISINFECTANTS Dr. Husam Aldeen Salim General information They have specific use and their selectivity is very low. Disinfectants

More information

11.1 Controlling Microorganisms

11.1 Controlling Microorganisms Chapter 11 Physical and Chemical Agents for Microbial Control* *Lecture notes are to be used as a study guide only and do not represent the comprehensive information you will need to know for the exams.

More information

10/6/2015. Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13) Aseptic Principles. Brain Check. Aseptic Principles

10/6/2015. Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13) Aseptic Principles. Brain Check. Aseptic Principles Aseptic Principles Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13) Sterilization: the killing or removal of all microbes in a material or on an object Disinfection: the

More information

Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13)

Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13) Aseptic Principles Unit 4: Sterilization, Disinfection, & Antimicrobial Therapy (Chapters 12 & 13) Sterilization: the killing or removal of all microbes in a material or on an object Disinfection: the

More information

Microbiology sheet (6)

Microbiology sheet (6) Microbiology sheet (6) Made by marah marahleh corrected by : abd. Salman DATE :9/10/2016 Microbial growth / control of microbial growth 1 The method of counting bacteria is divided into: 1) direct 2) indirect

More information

The Control of Microorganisms LC D R B R I A N B E A R D E N, M S, P E

The Control of Microorganisms LC D R B R I A N B E A R D E N, M S, P E The Control of Microorganisms LC D R B R I A N B E A R D E N, M S, P E U S P U B L I C H E A LT H S E R V I C E / U S E PA R 9 M A R I A N A I S L A N D S WAT E R O P E R ATO R S A S S O C I AT I O N F

More information

Principle of Lab. Safety

Principle of Lab. Safety Sulaimani University College of Pharmacy Principle of Lab. Safety Dr. Abdullah Ahmed Hama 1 1. Wear appropriate clothing and shoes to the laboratory. Shoes must completely cover the feet to provide protection

More information

number Done by Corrected by Doctor Hamed Al Zoubi

number Done by Corrected by Doctor Hamed Al Zoubi number 6 Done by Narjes Alsammak Corrected by Salsabeel Fleifel Doctor Hamed Al Zoubi Sterilization and disinfection Terms: -Sterilization: Elimination or deactivation of all forms of life (biological

More information

Lab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic

Lab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative

More information

2120 Lab. Week 11. Experiments 13,14,21. Kirby Bauer, TDT, Chemicals

2120 Lab. Week 11. Experiments 13,14,21. Kirby Bauer, TDT, Chemicals 2120 Lab Week 11 Experiments 13,14,21 Kirby Bauer, TDT, Chemicals Controlling Microorganisms Decontamination: Physical, chemical, and mechanical methods to destroy or reduce undesirable microbes in a given

More information

INTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants

INTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly

More information

Sterilization & Disinfection

Sterilization & Disinfection Sterilization & Disinfection Prof. Hanan Habib College of Medicine-KSU Objectives 1- Define the terms sterilization, disinfectant and antiseptic. 2- Classify the different methods of sterilization (physical

More information

Agent Mechanisms of Action Comments Surfactants. Membrane Disruption; increased penetration Denature proteins; Disrupts lipids

Agent Mechanisms of Action Comments Surfactants. Membrane Disruption; increased penetration Denature proteins; Disrupts lipids Agent Mechanisms of Action Comments Surfactants Quats (cationic detergent) Organic acids and bases Membrane Disruption; increased penetration Denature proteins; Disrupts lipids High/low ph Soaps; detergents

More information

DOWNLOAD PDF CONTROL OF MICROBES BY PHYSICAL AND CHEMICAL METHODS

DOWNLOAD PDF CONTROL OF MICROBES BY PHYSICAL AND CHEMICAL METHODS Chapter 1 : Physical and Chemical Methods of Control Clinical Gate Chemical Methods of Control Most reduce the microbial populations to safe levels or remove pathogens from objects. An ideal disinfectant

More information

Lec.5 Food Microbiology Dr. Jehan Abdul Sattar

Lec.5 Food Microbiology Dr. Jehan Abdul Sattar Lec.5 Food Microbiology Dr. Jehan Abdul Sattar High-Temperature Food Preservation: The use of high temperatures to preserve food is based on their destructive effects on microorganisms. Depending on the

More information

MICROBIOLOGICAL PROFILE

MICROBIOLOGICAL PROFILE Evans Vanodine International plc G L O B A L H Y G I E N E S O L U T I O N S APEX MICROBIOLOGICAL PROFILE 2 CONTENTS PAGE INTRODUCTION 3 1 BACTERICIDAL ACTIVITY 4 The following bacteria tested Campylobacter

More information

Choosing an Effective Sanitizer

Choosing an Effective Sanitizer Choosing an Effective Sanitizer, CEO - Shepard Bros., Inc. NWFSS 2017 Outline Antimicrobial Definitions Cleaning Comes First Biofilms Overview of Sanitizers Common Products New Technologies Organic Processing

More information

Control of Microorganisms by Physical and Chemical Agents

Control of Microorganisms by Physical and Chemical Agents CHAPTER 7 Control of Physical and Chemical Outline Concepts Bacteria are trapped on the surface of a membrane filter used to remove microorganisms from fluids. 7.1 Definition of Frequently Used Terms 137

More information

Exercise 24-A MICROBIAL CONTROL METHODS (Effects Of Temperature, Ultra Violet Light, Disinfectants And Antiseptics)

Exercise 24-A MICROBIAL CONTROL METHODS (Effects Of Temperature, Ultra Violet Light, Disinfectants And Antiseptics) Introduction Exercise 24-A MICROBIAL CONTROL METHODS (Effects Of Temperature, Ultra Violet Light, Disinfectants And Antiseptics) Microorganisms, like all other life forms, are greatly influenced by the

More information

Physical)and)Chemical)Control) of)microbes) Chapter)9) ) Rela8ve)Resistance)of)Different)Microbial)Types)to) Microbial)Control)Agents) More resistant

Physical)and)Chemical)Control) of)microbes) Chapter)9) ) Rela8ve)Resistance)of)Different)Microbial)Types)to) Microbial)Control)Agents) More resistant PhysicalandChemicalControl ofmicrobes Chapter9 Rela8veResistanceofMicrobialForms Primary*targets*of*microbialcontrolare*microorganisms* capable*of*causing*infecaon*or*spoilage* Microbes*can*have*extreme*differences*in*resistance*and*

More information

Principles of Preservation

Principles of Preservation From ISP/Sutton Laboratories Principles of Preservation What is preservation? When we speak as formulators about preservation, we are referring to the protection of our products from contamination by bacteria,

More information

Control and Sterilization (see pages )

Control and Sterilization (see pages ) Control and Sterilization (see pages 671-698) Some definitions: Sterilization: Killing of all life. No cells living or capable of becoming metabolically active Disinfection: A clinical term. Removal of

More information

2/25/2013. Psychrotrophs Grow between 0 C and C Cause food spoilage Food Preservation Temperatures

2/25/2013. Psychrotrophs Grow between 0 C and C Cause food spoilage Food Preservation Temperatures 3 4 5 6 7 8 9 0 Chapter 6 Microbial Growth Microbial Growth Increase in number of cells, not cell size Populations Colonies The Requirements for Growth Physical requirements Temperature ph Osmotic pressure

More information

Microbiology with Diseases by Body System Robert W. Bauman Third Edition

Microbiology with Diseases by Body System Robert W. Bauman Third Edition Microbiology with Diseases by Body System Robert W. Bauman Third Edition Pearson Education Limited Edinburgh Gate Harlow Essex CM20 2JE England and Associated Companies throughout the world Visit us on

More information

Antiseptics and Disinfectants

Antiseptics and Disinfectants A BCC Research Chemical Report CHM052B Use this report to: Understand current and future characteristics of the global market for antiseptics and disinfectants Learn about various categories of antiseptics

More information

LECTURE 4. MICROBIAL GROWTH

LECTURE 4. MICROBIAL GROWTH LECTURE 4. MICROBIAL GROWTH 1. Microbial survival strategies 2. Microbial growth 3. Effects of the environment 4. Microbial cultures 5. Growth measurements 6. Antimicrobial agents 7. Antibiotics 1. MICROBIAL

More information

Heat Sterilization. Module- 40 Lec- 40. Dr. Shishir Sinha Dept. of Chemical Engineering IIT Roorkee

Heat Sterilization. Module- 40 Lec- 40. Dr. Shishir Sinha Dept. of Chemical Engineering IIT Roorkee Heat Sterilization Module- 4 Lec- 4 Dr. Shishir Sinha Dept. of Chemical Engineering IIT Roorkee Sterilization is the total elimination of all microorganisms including spores Typically the last things to

More information

CHAPTER-V STERILIZATION R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR.

CHAPTER-V STERILIZATION R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR. CHAPTER-V STERILIZATION R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR. STERILIZATION Sterilization:- It is defined as the process where

More information

Sterilization and Disinfection

Sterilization and Disinfection Sterilization and Disinfection Sterilization: It is a process that kills all living microorganisms. Disinfection: It is a process that reduce the number of microorganisms (Unable to destroy spores and

More information

Dr. Gary Mumaugh. Microbial Control and Growth

Dr. Gary Mumaugh. Microbial Control and Growth Dr. Gary Mumaugh Microbial Control and Growth Microbial Growth Microbial Control Sterilization Selective Removal Temperature Types of Heat Treatment Incineration Tyndallization Autoclaving Microbial Control

More information

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly

More information

Control of Microorganisms in the Environment and Antimicrobial Chemotherapy

Control of Microorganisms in the Environment and Antimicrobial Chemotherapy 8 and 9 Control of Microorganisms in the Environment and Antimicrobial Chemotherapy Copyright McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display. 1 2 Definition

More information

6/28/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics

6/28/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical

More information

Chapter 6: Microbial Growth

Chapter 6: Microbial Growth Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends

More information

HANDSAN MICROBIOLOGICAL PROFILE

HANDSAN MICROBIOLOGICAL PROFILE HANDSAN MICROBIOLOGICAL PROFILE 1 CONTENTS PAGE INTRODUCTION 2 1 BACTERICIDAL ACTIVITY 3-6 The following bacteria tested Acinetobacter baumannii Enterococcus hirae Escherichia coli '0157'' Escherichia

More information

Control of Micro-Organisms

Control of Micro-Organisms Control of Micro-Organisms Physical Chemical Antibiotics and Chemotherapeutics Physical Control of Microorganisms GOAL = STERILIZATION: removal of all life forms, inc. bacterial spores, viruses, etc. 2004

More information

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly

More information

M I C R O B I O L O G Y

M I C R O B I O L O G Y ninth edition TORTORA FUNKE CASE M I C R O B I O L O G Y a n i n t r o d u c t i o n 6 Microbial Growth PowerPoint Lecture Slide Presentation prepared by Christine L. Case Microbial Growth Microbial growth

More information

Take-Home Quiz I. Please note that questions 1-25 cover material solely from Chapters 1 through 5.

Take-Home Quiz I. Please note that questions 1-25 cover material solely from Chapters 1 through 5. Take-Home Quiz I General Instructions and Information: Obtain an answer sheet from the instructor and legibly write your name in the appropriate space. After placing your name and quiz version on the answer

More information

20.106J Systems Microbiology Lecture 16 Prof. Schauer. Chapter 20

20.106J Systems Microbiology Lecture 16 Prof. Schauer. Chapter 20 20.106J Systems Microbiology Lecture 16 Prof. Schauer Chapter 20 Microbial growth control o Physical antimicrobial control o Chemicals that are used externally o Antimicrobial agents used internally o

More information

Biosecurity Sanitation and Pest Control

Biosecurity Sanitation and Pest Control Biosecurity Sanitation and Pest Control Animal Biosecurity All measures used to control all known or unknown infections in laboratory animals. All measures taken to identify, contain, prevent, and eradicate

More information

Disinfection and sterilisation

Disinfection and sterilisation Disinfection and sterilisation Mongolia 2011 Prof. Dr. Walter Popp Hospital Hygiene, University Clinics Essen, Germany 1 Term Definition Reduction factor of germs Cleaning Remove dirt including 10-100

More information

COLLEGE OF PHARMACY STERILE PRODUCTS PHT 434. Dr. Mohammad Javed Ansari, PhD. Contact info:

COLLEGE OF PHARMACY STERILE PRODUCTS PHT 434. Dr. Mohammad Javed Ansari, PhD. Contact info: COLLEGE OF PHARMACY STERILE PRODUCTS PHT 434 Dr. Mohammad Javed Ansari, PhD. Contact info: javedpharma@gmail.com OBJECTIVES OF THE LECTURE At the end of this lecture, you will be aware of: What are Sterile

More information

10/2/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics

10/2/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical

More information

Tissue cleaning and sterilization provide an additional level of safety

Tissue cleaning and sterilization provide an additional level of safety Managing biologics Understanding tissue processing Third in a series on managing bone allografts. In the October issue, articles included were Allografts: Overview of the process; and Donor screening:

More information

Environmental microbi BIOSCI204 FINAL EXAM PREPARATION. Microbiology in nutrition, growth and metabolism

Environmental microbi BIOSCI204 FINAL EXAM PREPARATION. Microbiology in nutrition, growth and metabolism BIOSCI204 FINAL EXAM PREPARATION Microbiology in nutrition, growth and metabolism Microbial nutrition Microbial growth Control of microorganism in the environment Microbial metabolism: Energy, enzymes

More information

Eng. Joyce Haddad Abboud

Eng. Joyce Haddad Abboud Food and Hospitality Oman Eng. Joyce Haddad Abboud Food Industrial Engineer / Sanitary Engineer General Coordinator of the food safety campaign in Lebanese Ministry of Public Health 1 Understanding what

More information

The Complete Solution for Clean Room Aerosol-Based Disinfection MINNCARE DRY FOG SYSTEM

The Complete Solution for Clean Room Aerosol-Based Disinfection MINNCARE DRY FOG SYSTEM The Complete Solution for Clean Room Aerosol-Based Disinfection Current Methods of Room Disinfection Surface Wiping Manual Spraying (with Spray Bottles) Heating Process (Vaporization) Cold Process - Wet

More information

Physical and Chemical Control of Microbes Chapter 9

Physical and Chemical Control of Microbes Chapter 9 Physical and Chemical Control of Microbes Chapter 9 Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Controlling Microorganisms Controlling our degree of exposure

More information

Water sanitation at its best

Water sanitation at its best Water sanitation at its best - www.basu.at This comparative study shows the clear advantage of over the competition Chlorine Is used in its gaseous state and requires strict security measures Powerful

More information

Take-Home Quiz II. Summer 2005 Semester

Take-Home Quiz II. Summer 2005 Semester General Instructions and Information: Obtain an answer sheet from the instructor and legibly write your name in the appropriate space. After placing your name, you must enter your Patron ID Number (NOT

More information