DNA Technology Outline
|
|
- Andrew Tyler
- 5 years ago
- Views:
Transcription
1 I) Tools of DNA technology A. PCR (Polymerase Chain Reaction): method of copying DNA sequences 1. DNA is copied in a similar way to natural replication in our cells, but much faster.
2 2.PCR consists of cycles of the following three steps: a.dna is heated up until complementary strands separate (called denaturation). b.as the DNA cools, special nucleic acids called primers attach to the separated DNA strands. Primers are used to target specific sequences for copying. c.dna polymerase starts at the primers and adds complementary nucleotides down the length of the DNA strand. PCR Each cycle doubles the amount of DNA present.
3 B. Restriction Enzymes: specialized proteins used to cut DNA 1. A restriction enzyme recognizes one specific DNA sequence. 2. When a restriction enzyme encounters the target sequence, it will cut the DNA in that spot only. 3.Many restriction enzymes do not cut straight through both strands, leaving what are called sticky ends as shown below: 4.Sticky ends are very useful to scientists that are interested in moving a gene from one organism into another.
4 C. Gel Electrophoresis: process of separating DNA fragments using an electric current 1. DNA is negatively charged, therefore it will be attracted to a positive charge. 2. If a DNA sample is placed in a solid, but flexible gel, it can be pulled through by an electrical current. 3.Long fragments of DNA are much more difficult to move through the gel, so they will not travel as far as short fragments. 4.The end result of gel electrophoresis is a separation of DNA fragments based on their size.
5 II) Biotech in Forensics A. Making a DNA Fingerprint 1.If biological evidence is left at a crime scene (blood, hair, saliva, etc.), detectives can collect it and send it to the lab. The lab needs to copy the DNA using PCR so that they have enough to do their testing. 2. After PCR, the DNA is cut with restriction enzymes. 3. Gel electrophoresis is used to separate the fragments of DNA based on their size.
6 4.Scientists stain the gel with a chemical that makes DNA glow under UV light, then take a picture of the banding patterns in the gel. This picture is called a DNA fingerprint. Gel electrophoresis Which suspect is probably the attacker? How do you know?
7 B. Interpreting a DNA fingerprint 1.Each person (except for identical twins) has his or her own unique DNA sequence. 2.Since everyone s DNA sequence differs, if you use the same restriction enzyme on two different people, it may cut the DNA is different places, producing DNA fragments of different lengths. 3.When those DNA fragments are run on a gel, they will produce a unique pattern of bands based on the lengths of the fragments present. 4.If a suspect s DNA fingerprint matches the pattern of the DNA from the crime scene, that is solid evidence to place the suspect at the scene of the crime.
8 III) Biotech in Medicine A. Genetic Engineering: any deliberate alteration of the genetic material of an organism 1.Recombinant DNA: DNA that results from a gene of one organism being placed into the DNA of another Example: People with diabetes lack the insulin necessary to regulate their blood sugar. In order to get the insulin they need, it was traditionally harvested from cows and/or pigs that were slaughtered for food. This is very expensive and time-consuming.
9 E. coli is a bacterium that is very easy to grow in the lab. Therefore, researchers have taken the gene for human insulin and have inserted it into E. coli cells, producing bacteria that are able to manufacture insulin very quickly and inexpensively. B. Cloning: process of producing an organism that is an exact genetic copy of the original 1.Most common method of artificial cloning is called Somatic Cell Nuclear Transfer (SCNT) This is how Dolly the sheep was created.
10 a.an unfertilized egg is removed from a female. b.the nucleus is removed from the egg. c.the nucleus from a somatic cell (diploid body cell=not a gamete) of a donor organism is removed and placed into the now empty egg. d.the egg is chemically or electrically stimulated to divide as if it had just been fertilized. e.the egg is placed into a surrogate mother, who will carry it to term and deliver the clone.
11 2. Cloning can happen in nature as well (identical twins, fragmentation of a starfish, binary fission in bacteria) C. Stem Cell Research 1.Stem cells are important because they have the ability to differentiate into many different types cells. There are two categories of stem cells:
2 Gene Technologies in Our Lives
CHAPTER 15 2 Gene Technologies in Our Lives SECTION Gene Technologies and Human Applications KEY IDEAS As you read this section, keep these questions in mind: For what purposes are genes and proteins manipulated?
More informationChapter 11: Applications of Biotechnology
Chapter 11: Applications of Biotechnology Lecture Outline Enger, E. D., Ross, F. C., & Bailey, D. B. (2012). Concepts in biology (14th ed.). New York: McGraw- Hill. 11-1 Why Biotechnology Works 11-2 Biotechnology
More information13-1 Changing the Living World
13-1 Changing the Living World In the past, variation was limited to the variations already in nature or random variations that resulted from mutations. Now, scientists can change DNA and swap genes from
More information-Is the process of manipulating genes and genomes
Genetic Engineering -Is the process of manipulating genes and genomes Biotechnology -Is the process of manipulating organisms or their components for the purpose of making useful products Restriction Enzymes
More informationWhat is Biotechnology? 15.1 What is Biotechnology? Transgenic Biotechnology Transgenic Biotechnology. Biotechnology. Transgenic organism
What is Biotechnology? 15.1 What is Biotechnology? Biotechnology the use of technology to control biological processes as a means of meeting societal needs Gene therapy Genetic engineering Bioremediation
More informationRecombinant DNA recombinant DNA DNA cloning gene cloning
DNA Technology Recombinant DNA In recombinant DNA, DNA from two different sources, often two species, are combined into the same DNA molecule. DNA cloning permits production of multiple copies of a specific
More informationNOTES - CH 15 (and 14.3): DNA Technology ( Biotech )
NOTES - CH 15 (and 14.3): DNA Technology ( Biotech ) Vocabulary Genetic Engineering Gene Recombinant DNA Transgenic Restriction Enzymes Vectors Plasmids Cloning Key Concepts What is genetic engineering?
More informationBIOTECHNOLOGY. Understanding the Application
BELLRINGER-5/4/15 1. What method would you guess forensic scientists use to identify criminals at crime scenes? 2. What do you think we mean by the term biotechnology? BIOTECHNOLOGY Understanding the Application
More informationManipulating DNA. Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates.
Lesson Overview 14.3 Studying the Human Genome Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates. Nucleic acids are chemically different from other macromolecules
More informationBIOTECHNOLOGY. Understanding the Application
BIOTECHNOLOGY Understanding the Application GENETIC ENGINEERING Genetic engineering refers to any process in which man alters an organism s DNA Examples: cloning, genetically modified organisms (GMO),
More information12/31/16. I. Manipulating DNA (9.1) A. Scientists use several techniques to manipulate DNA. 1. DNA is a very large molecule
I. Manipulating DNA (9.1) A. Scientists use several techniques to manipulate DNA 1. DNA is a very large molecule 3. Led to many biotechnology applications- genetic engineering, DNA fingerprinting, cloning,
More informationChapter 15 Gene Technologies and Human Applications
Chapter Outline Chapter 15 Gene Technologies and Human Applications Section 1: The Human Genome KEY IDEAS > Why is the Human Genome Project so important? > How do genomics and gene technologies affect
More informationUNIT III: Genetics Chapter 9 Frontiers of Biotechnology
UNIT III: Genetics Chapter 9 Frontiers of Biotechnology I. Manipulating DNA (9.1) A. Scientists use several techniques to manipulate DNA 1. DNA is a very large molecule 2. Still to small to see or work
More informationOverview: The DNA Toolbox
Overview: The DNA Toolbox Sequencing of the genomes of more than 7,000 species was under way in 2010 DNA sequencing has depended on advances in technology, starting with making recombinant DNA In recombinant
More information4/26/2015. Cut DNA either: Cut DNA either:
Ch.20 Enzymes that cut DNA at specific sequences (restriction sites) resulting in segments of DNA (restriction fragments) Typically 4-8 bp in length & often palindromic Isolated from bacteria (Hundreds
More informationCopyright 2002 Pearson Education, Inc., publishing as Benjamin Cummings
Here s one thing genetic engineers do: Techniques for gene cloning enable scientists to prepare multiple identical copies of gene-sized pieces of DNA. Cloning means to make copies, in this case, copies
More informationRestriction Enzymes (endonucleases)
In order to understand and eventually manipulate DNA (human or otherwise) an array of DNA technologies have been developed. Here are some of the tools: Restriction Enzymes (endonucleases) In order to manipulate
More informationCloning and Genetic Engineering
Cloning and Genetic Engineering Bởi: OpenStaxCollege Biotechnology is the use of artificial methods to modify the genetic material of living organisms or cells to produce novel compounds or to perform
More informationGenetics and Biotechnology 13.2 DNA Technology
Biotechnology Genetic Engineering Technology that involves manipulating the DNA of one organism in order to insert the DNA of another organism An electric current is used to separate DNA fragments according
More informationUNIT 3: GENETICS Chapter 9: Frontiers of Biotechnology
CORNELL NOTES Directions: You must create a minimum of 5 questions in this column per page (average). Use these to study your notes and prepare for tests and quizzes. Notes will be stamped after each assigned
More informationRecombinant DNA. Lesson Overview. Lesson Overview Recombinant DNA
Lesson Overview 15.2 Finding Genes In 1987, Douglas Prasher, a biologist at Woods Hole Oceanographic Institute in Massachusetts, wanted to find a specific gene in a jellyfish that codes for a molecule
More informationRevision Based on Chapter 15 Grade 10
Revision Based on Chapter 15 Grade 10 Biology Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Which of the following has the disadvantage of possibly bringing
More informationBiotechnology: Genetic Engineering
Biotechnology: Genetic Engineering What genetic traits are being expressed? https://www.youtube.com/watch?v=xlqrg08af1u Do Now: Write your homework down and collect it from the front. Labeling GMO Salmon
More information9.4. Genetic Engineering. Entire organisms can be cloned. Web
9.4 Genetic Engineering VOCABULARY clone genetic engineering recombinant DNA plasmid transgenic gene knockout 3D, 3D evaluate the impact of scientific research on society and the environment and 6H describe
More informationBiology Chapter 9 & Honors Biology Chapter 13. Frontiers Of Biotechnology
Biology Chapter 9 & Honors Biology Chapter 13 Frontiers Of Biotechnology DNA TECHNOLOGY IS ABOUT: Manipulating DNA for man s purposes. It includes: cutting DNA, Gel Electrophoresis and Polymerase Chain
More informationGenetic Engineering RESTRICTION ENDONUCLEASES
Genetic Engineering 1977 Frederick Sanger discovered the complete base sequence for one type of virus, identified all 9 of its genes, and became the first to do so. This opened up a whole new world for
More informationTopic 3 Genetics/ Biotechnology
Topic 3 Genetics/ Biotechnology 3.5.U2 PCR can be used to amplify small amounts of DNA. 1. The polymerase chain reaction (PCR) is used where DNA samples are too small to be useful. a. State the purpose
More informationResearchers use genetic engineering to manipulate DNA.
Section 2: Researchers use genetic engineering to manipulate DNA. K What I Know W What I Want to Find Out L What I Learned Essential Questions What are the different tools and processes used in genetic
More information13-2 Manipulating DNA Slide 1 of 32
1 of 32 The Tools of Molecular Biology The Tools of Molecular Biology How do scientists make changes to DNA? Scientists use their knowledge of the structure of DNA and its chemical properties to study
More informationVirus- infectious particle consisting of nucleic acid packaged in a protein coat.
Chapter 19 Virus- infectious particle consisting of nucleic acid packaged in a protein coat. Most scientists consider viruses non-living because they cannot reproduce or carry out metabolic activities
More informationUnderstandings, Applications and Skills (This is what you maybe assessed on)
3. Genetics 3.5 Genetic modification and biotechnology Name: Understandings, Applications and Skills (This is what you maybe assessed on) Statement Guidance 3.5.U1 3.5.U2 3.5.U3 Gel electrophoresis is
More informationCHAPTER 13. Genetic Engineering
CHAPTER 13 Genetic Engineering 13-1 Changing the Living World Choosing the best traits for breeding Most domestic animals are products of SB Even Cows, Sheep, & Pigs All Products of Selective Breeding
More informationGENETICS HOW HUMANS MANIPULATE GENES FOR THEIR OWN BENEFIT
GENETICS HOW HUMANS MANIPULATE GENES FOR THEIR OWN BENEFIT SELECTIVE BREEDING &HYBRIDIZATION CHOOSING ORGANISMS OF THE SAME SPECIES WITH THE BEST TRAITS TO MATE TOGETHER Examples: Purebred dogs, horses,
More informationBiotechnology DNA technology
Biotechnology Biotechnology is the manipulation of organisms or their components to make useful products The applications of DNA technology affect everything from agriculture, to criminal law, to medical
More informationBIOTECHNOLOGY. Unit 8
BIOTECHNOLOGY Unit 8 PART 1 BASIC/FUNDAMENTAL SCIENCE VS. APPLIED SCIENCE! Basic/Fundamental Science the development and establishment of information to aid our understanding of the world.! Applied Science
More informationA cross between dissimilar individuals to bring together their best characteristics is called
Ch 13 Game review A cross between dissimilar individuals to bring together their best characteristics is called A Genetic engineering B Inbreeding C Hybridization D Sequencing Ans: C Used to insert new
More informationAdvances in Genetics #101
Questions: Five study Questions EQ1: What are 3 ways of producing organisms with desired traits? EQ2: What are 2 applications of DNA technology in human genetics? Like your fingerprints, your DNA is different
More informationDNA, or Deoxyribonucleic Acid, is the genetic material in our cells. No two people (except identical twins) have the
DNA, or Deoxyribonucleic Acid, is the genetic material in our cells. No two people (except identical twins) have the exact same DNA. DNA patterns from four sets of twins which are identical? DNA fingerprinting
More informationDNA Function. DNA Heredity and Protein Synthesis
DNA Function DNA Heredity and Protein Synthesis 1 Review DNA made of Nucleotide bases Proteins made of Amino acids Describe how DNA is involved in protein synthesis DNA base sequence codes for amino acid
More informationGenetic Technologies.notebook March 05, Genetic Technologies
Genetic Testing Genetic Technologies Tests can be used to diagnose disorders and/or identify those individuals with an increased risk of inheriting a disorder. Prenatal Screening A fetus may be screened
More informationBio 101 Sample questions: Chapter 10
Bio 101 Sample questions: Chapter 10 1. Which of the following is NOT needed for DNA replication? A. nucleotides B. ribosomes C. Enzymes (like polymerases) D. DNA E. all of the above are needed 2 The information
More informationBiosc10 schedule reminders
Biosc10 schedule reminders Review of molecular biology basics DNA Is each person s DNA the same, or unique? What does DNA look like? What are the three parts of each DNA nucleotide Which DNA bases pair,
More informationBiotechnology Chapter 20
Biotechnology Chapter 20 DNA Cloning DNA Cloning AKA Plasmid-based transformation or molecular cloning First off-let s sum up what happens. A plasmid is taken from a bacteria A gene is inserted into the
More informationAgenda (Monday-Wednesday)
Agenda (Monday-Wednesday) Chapter 12 Recombinant DNA Technology Recombinant DNA Techniques DNA Fingerprinting and Forensic Science DNA Fingerprinting Techniques Pre-lab 8 activities Tomorrow: Day One of
More informationI. Gene Cloning & Recombinant DNA. Biotechnology: Figure 1: Restriction Enzyme Activity. Restriction Enzyme:
I. Gene Cloning & Recombinant DNA Biotechnology: Figure 1: Restriction Enzyme Activity Restriction Enzyme: Most restriction enzymes recognize a single short base sequence, or Restriction Site. Restriction
More informationChemical treatments cause cells and nuclei to burst The DNA is inherently sticky, and can be pulled out of the mixture This is called spooling DNA
DNA Technology 1 2 DNA Extraction Chemical treatments cause cells and nuclei to burst The DNA is inherently sticky, and can be pulled out of the mixture This is called spooling DNA Spooled DNA 3 4 Cutting
More informationGenetic Technologies
Genetic Technologies Distinguish the terms biotechnology, recombinant DNA technology, transgenic organisms, genetic engineering Understand the two basic techniques to obtain selective fragments of DNA
More informationGene Expression. Chapters 11 & 12: Gene Conrtrol and DNA Technology. Cloning. Honors Biology Fig
Chapters & : Conrtrol and Technology Honors Biology 0 Cloning Produced by asexual reproduction and so it is genetically identical to the parent st large cloned mammal: Dolly the sheep Animals that are
More informationDNA Technology. B. Using Bacteria to Clone Genes: Overview:
DNA Technology A. Basic Vocabulary: is DNA from 2 different sources that is combined. is the direct manipulation of genes for practical purposes. literally means or in a test tube or flask. is the manipulation
More informationDNA REPLICATION & BIOTECHNOLOGY Biology Study Review
DNA REPLICATION & BIOTECHNOLOGY Biology Study Review DNA DNA is found in, in the nucleus. It controls cellular activity by regulating the production of, which includes It is a very long molecule made up
More informationChapter 10 Genetic Engineering: A Revolution in Molecular Biology
Chapter 10 Genetic Engineering: A Revolution in Molecular Biology Genetic Engineering Direct, deliberate modification of an organism s genome bioengineering Biotechnology use of an organism s biochemical
More informationB. Incorrect! Ligation is also a necessary step for cloning.
Genetics - Problem Drill 15: The Techniques in Molecular Genetics No. 1 of 10 1. Which of the following is not part of the normal process of cloning recombinant DNA in bacteria? (A) Restriction endonuclease
More informationUnit 3.notebook June 03, Genetic Counseling. May 11 12:18 PM. Genetic Counseling
Genetic Counseling Until recently, it was very difficult to determine the health of an unborn baby. Today, with new research and technology, information can be gathered during: > fetal development > before
More informationName Date Class CHAPTER 13. DNA Fingerprinting
Real-World Biology: Analysis DNA Fingerprinting Genetic Prints Help Solve Mystery of Girls Switched at Birth. Murder Conviction Overturned by DNA Testing: Prisoner Released. Headlines such as these have
More informationFurther Reading - DNA
Further Reading - DNA DNA BACKGROUND What is DNA? DNA (short for deoxyribonucleic acid ) is a complex molecule found in the cells of all living things. The blueprint for life, DNA contains all the information
More informationGuided Notes Unit 5: Molecular Genetics
Name: Date: Block: Chapter 8: From DNA to Protein I. Concept 8.4: Transcription a. Central Dogma of Molecular Biology i. Information flows in one direction: ii. How? Guided Notes Unit 5: Molecular Genetics
More informationAllele: Chromosome DNA fingerprint: Electrophoresis: Gene:
Essential Vocabulary Allele: an alternate form of a gene; for example, a gene for human hair color may have alleles that cause red or brown hair Chromosome: a cell structure that contains genetic information
More information2014 Pearson Education, Inc. CH 8: Recombinant DNA Technology
CH 8: Recombinant DNA Technology Biotechnology the use of microorganisms to make practical products Recombinant DNA = DNA from 2 different sources What is Recombinant DNA Technology? modifying genomes
More informationName Class Date. a. identify similarities and
Chapter 13 enetic Engineering Chapter Test A Multiple Choice Write the letter that best answers the question or completes the statement on the line provided. 1. Selective breeding produces a. more offspring.
More informationDNA Analysis Students will learn:
DNA Analysis Students will learn: That DNA is a long-chain polymer found in nucleated cells, which contain genetic information. That DNA can be used to identify or clear potential suspects in crimes. How
More informationTOPIC BIOTECHNOLOGY
TOPIC 3.5 - BIOTECHNOLOGY 3.5 A Techniques & Profiling IB BIO 3.5 3 Understandings U1: Gel electrophoresis is used to separate proteins or fragments of DNA according to size. Gel electrophoresis is a technique
More informationBIOLOGY - CLUTCH CH.20 - BIOTECHNOLOGY.
!! www.clutchprep.com CONCEPT: DNA CLONING DNA cloning is a technique that inserts a foreign gene into a living host to replicate the gene and produce gene products. Transformation the process by which
More informationDNA Profiling. (DNA fingerprinting)
DNA Profiling (DNA fingerprinting) Background Information: Restriction Enzymes Restriction Enzymes Evolved by bacteria to protect against viral DNA infection. Also called Endonucleases. They cleave DNA
More informationFun with DNA polymerase
Fun with DNA polymerase Why would we want to be able to make copies of DNA? Can you think of a situation where you have only a small amount and would like more? Enzymatic DNA synthesis To use DNA polymerase
More informationCH 8: Recombinant DNA Technology
CH 8: Recombinant DNA Technology Biotechnology the use of microorganisms to make practical products Recombinant DNA = DNA from 2 different sources What is Recombinant DNA Technology? modifying genomes
More informationChapter 13. Genetic Engineering
Chapter 13 Genetic Engineering Selective Breeding Passing on desired characteristics to the next generation. Examples: different breeds of domestic and farm animals, different varieties of plants (corn,
More informationDNA. Shape = Double Helix (twisted ladder) The purpose of each cell having DNA is to have directions for the cell to make proteins
DNA DNA Deoxyribo- Nucleic Acid Shape = Double Helix (twisted ladder) The purpose of each cell having DNA is to have directions for the cell to make proteins Parts = nucleotide 1. Sugar (deoxyribose) 2.
More informationBiology 3201 Genetics Unit #8
Biology 3201 Genetics Unit #8 Diagnosis and Treatment of Genetic Disorders Genetic Engineering The Human Genome Project GMOs and GMFs Cloning Diagnosis of Genetic Disorders Detection of genetics disorders-
More informationHuman Genomics. 1 P a g e
Human Genomics What were the aims of the human genome project? To identify all the approximately 20,000-25,000 genes in Human DNA. To find where each gene is located To determine the sequences of the 3
More informationGenetic tests are available for hundreds of disorders. DNA testing can pinpoint the exact genetic basis of a disorder.
Human DNA Analysis Human DNA Analysis There are roughly 6 billion base pairs in your DNA. Biologists search the human genome using sequences of DNA bases. Genetic tests are available for hundreds of disorders.
More informationGenetic Engineering : (page 613)
Genetic Engineering : (page 613) 1977 - Frederick Sanger - discovered the complete base sequence for one type of virus, identified all 9 of its genes, first to do so...opening a new world for genetic procedures
More informationBiology Warm Up. 1. Complete the entrance ticket you received at the door.
Biology Warm Up Monday, February 8 1. Complete the entrance ticket you received at the door. NOTE: This is not a grade. I want to see what you know/remember. Once you finish, place in front blue basket.
More informationDNA stands for deoxyribose nucleic acid DNA is a very large molecule made up of a long chain of sub-units The sub-units are called nucleotides Each
1 DNA stands for deoxyribose nucleic acid DNA is a very large molecule made up of a long chain of sub-units The sub-units are called nucleotides Each nucleotide is made up of a sugar called deoxyribose
More informationBIOTECHNOLOGY. Sticky & blunt ends. Restriction endonucleases. Gene cloning an overview. DNA isolation & restriction
BIOTECHNOLOGY RECOMBINANT DNA TECHNOLOGY Recombinant DNA technology involves sticking together bits of DNA from different sources. Made possible because DNA & the genetic code are universal. 2004 Biology
More informationPart I: Predicting Genetic Outcomes
Part I: Predicting Genetic Outcomes Deoxyribonucleic acid (DNA) is found in every cell of living organisms, and all of the cells in each organism contain the exact same copy of that organism s DNA. Because
More information2054, Chap. 14, page 1
2054, Chap. 14, page 1 I. Recombinant DNA technology (Chapter 14) A. recombinant DNA technology = collection of methods used to perform genetic engineering 1. genetic engineering = deliberate modification
More informationMutations, Genetic Testing and Engineering
Mutations, Genetic Testing and Engineering Objectives Describe how techniques such as DNA fingerprinting, genetic modifications, and chromosomal analysis are used to study the genomes of organisms (TEKS
More informationLecture 3 (FW) January 28, 2009 Cloning of DNA; PCR amplification Reading assignment: Cloning, ; ; 330 PCR, ; 329.
Lecture 3 (FW) January 28, 2009 Cloning of DNA; PCR amplification Reading assignment: Cloning, 240-245; 286-87; 330 PCR, 270-274; 329. Take Home Lesson(s) from Lecture 2: 1. DNA is a double helix of complementary
More informationThe Techniques of Molecular Biology: Forensic DNA Fingerprinting
Revised Fall 2016 The Techniques of Molecular Biology: Forensic DNA Fingerprinting **Lab coat, eye goggles and gloves (nitrile or latex) are required for this lab. You will not be allowed to participate
More informationDefine selective breeding. Define pure breeding. Define domestication relative to the examples above.
Define selective breeding. Define pure breeding. Define domestication relative to the examples above. Selective Breeding Selective Breeding Induced nondisjunction Define hybridization. Explain how hybridization
More informationExploring Genetic Variation in a Caffeine Metabolism gene LAB TWO: POLYMERASE CHAIN REACTION
Exploring Genetic Variation in a Caffeine Metabolism gene LAB TWO: POLYMERASE CHAIN REACTION Purpose: In this laboratory, we will set up a polymerase chain reaction to amplify the region of the caffeine
More informationBiotechnology. Professor Andrea Garrison Biology 11 Illustrations 2010 Pearson Education, Inc., unless otherwise noted
Biotechnology Professor Andrea Garrison Biology 11 Illustrations 2010 Pearson Education, Inc., unless otherwise noted Biotechnology Any technical procedure used to isolate, analyze or manipulate genes
More informationPre-AP Biology DNA and Biotechnology Study Guide #1
Last Name: First Name: Per. Pre-AP Biology DNA and Biotechnology Study Guide #1 Structure of DNA: Number of strands. Parallel or antiparallel?. Rosalind Franklin s x-ray crystallography image indicated
More informationPage 70 Monday December 8, 2014
replication and Monday December 8, 0 Notebook check 8: Page 69, DNA Technology Introduction Worksheet. The process by which a foreign gene is replicated by insertion into a bacterium is called genetic
More informationBiotechnology. Slide 1 / 50. Slide 2 / 50. Slide 3 / 50. Biotechnology Defined. Biotechnology is a Business
Slide 1 / 50 Biotechnology Biotechnology Defined Slide 2 / 50 The manipulation (as through genetic engineering) of living organisms or their components to produce useful, usually commercial products (as
More informationMolecular Cell Biology - Problem Drill 11: Recombinant DNA
Molecular Cell Biology - Problem Drill 11: Recombinant DNA Question No. 1 of 10 1. Which of the following statements about the sources of DNA used for molecular cloning is correct? Question #1 (A) cdna
More informationTues 1/21. Today: Virus movie clip, ek paragraph for ch 20. Next class: collect Ch. 20 Guided Reading
Tues 1/21 Today: Virus movie clip, ek paragraph for ch 20. Next class: collect Ch. 20 Guided Reading Pg. 104 Ch. 20 Guided Reading Pg. 105 EK Paragraph 3C3 Wed. 1/22 Collect-Ch 20 Guided Reading Today:
More informationPick up 3 handout from back. Draw this on the back side of the handout called Concepts of biotechnology. After you have read and highlighted
Pick up 3 handout from back. Draw this on the back side of the handout called Concepts of biotechnology. After you have read and highlighted important information from the reading, see if you cann identify
More informationIowa State FFA Biotechnology CDE Comprehensive Knowledge Test
Iowa State FFA Biotechnology CDE Comprehensive Knowledge Test - 2017 - Name Chapter 1. What is the name of the technique that allows geneticists to observe DNA fragments from a DNA sample and compare them
More informationSTUDY GUIDE SECTION 13-1 DNA Technology
STUDY GUIDE SECTION 13-1 DNA Technology Name Period Date Multiple Choice-Write the correct letter in the blank. 1. To cut DNA molecules into pieces at specific sequences of nucleotides, genetic engineers
More informationRecombinant DNA Technology. The Role of Recombinant DNA Technology in Biotechnology. yeast. Biotechnology. Recombinant DNA technology.
PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 8 Recombinant DNA Technology The Role of Recombinant DNA Technology in Biotechnology Biotechnology?
More informationWhat are clones? Genetically identical copies
Cloning What are clones? Genetically identical copies The possibility of human cloning was raised when Scottish scientists at Roslin Institute created the much-celebrated sheep "Dolly" Cloning Cloning
More informationName: Date: IF YOU GOT BELOW A 70% RETAKING THE TEST IS MANDATORY.
IF YOU GOT BELOW A 70% RETAKING THE TEST IS MANDATORY. 1. What is a mutation? Any change in a DNA sequence 2. Name and describe the two categories types of gene mutations. Point: substitution, one nucleotide
More informationGENETICS: BIOLOGY HSA REVIEW
GENETICS: BIOLOGY HSA REVIEW HSA Review A. Matching: On the lines provided, write the letter of the definition of each term. a. genetics f. gamete b. trait g. probability c. hybrid h. Punnett square d.
More informationMore often heard about on television dramas than on the news, DNA is the key to solving crimes the scientific way. Although it has only been
DNA Matching More often heard about on television dramas than on the news, DNA is the key to solving crimes the scientific way. Although it has only been relatively recent (compared the course of forensic
More informationFinal Review: Biotech Section
Name: attempt# Final Review: Biotech Section GENE MUTATIONS 1. Define mutation: 2. Define gene mutation: 3. What are the two categories of gene mutations? 4. Label the diagram with the different types
More informationAP Biology: Unit 5: Development. Forensic DNA Fingerprinting: Using Restriction Enzymes Bio-Rad DNA Fingerprinting Kit
Forensic DNA Fingerprinting: Using Restriction Enzymes Bio-Rad DNA Fingerprinting Kit Background: Scientists working in forensic labs are often asked to perform DNA profiling or fingerprinting to analyze
More informationPolymerase Chain Reaction PCR
Polymerase Chain Reaction PCR What is PCR? An in vitro process that detects, identifies, and copies (amplifies) a specific piece of DNA in a biological sample. Discovered by Dr. Kary Mullis in 1983. A
More informationInterpreting DNA Analysis
Unit 4 Genetics Investigation 4 Interpreting DN nalysis Background One of the most useful tools in forensic science is a technique for identifying small quantities of DN, which may be found as part of
More information