Premier qpcr Kit [SYBR Green with ROX]
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1 Premier qpcr Kit [SYBR Green with ROX] Beyond Imagination IN NOBIZ
![Premier qpcr Kit Premier qpcr Kit [SYBR Green with ROX] is designed to perform a rapid real-time quantification of target DNA or first strand cdna synthesized from](/docs-images/83/87271532/images/2-1.jpg "RNA molecule. The x premix contains SYBR Green I dye, ROX passive dye, antibody-mediated hot-start DNA polymerase, dntps, buffers, MgCl, stabilizers, and enhancers.")
2 Premier qpcr Kit Premier qpcr Kit [SYBR Green with ROX] is designed to perform a rapid real-time quantification of target DNA or first strand cdna synthesized from RNA molecule. The x premix contains SYBR Green I dye, ROX passive dye, antibody-mediated hot-start DNA polymerase, dntps, buffers, MgCl, stabilizers, and enhancers. Instrument compatibility SYBR Green with low ROX 75 (ABI) CFX-9 (Bio-Rad) Rotor-Gene Q (QIAGEN) Light r (Roche) Mx3P (Stratagene) / SYBR Green with high ROX 79HT, 73, 57 (ABI) StepOnePlus TM, StepOne TM (ABI) PMT technology applied Accurate and robust detection of target Minimize primer-dimers and non-specific amplifications
3 CT Concentration Experimental Data 1. R =.997 Efficiency= ng/rxn 3.3 ng/rxn.33 ng/rxn.33 ng/rxn Human genomic DNA (ng/rxn) Sensitive detection using Premier qpcr Kit [SYBR Green with low ROX]. 1-fold serial-diluted genomic DNA as the standard sample were analyzed using the Premier qpcr Kit for human glucose-- phosphate dehydrogenase gene-specific primer set, : Negative control. Housekeeping Gene Real-time PCR Kit Template GC (%) Amplification Data Real-time PCR Instruments..7 E=% R =.99 hhprt-1 (Hypoxanthine quanine phosphoribosyl transferase 1) hhmbs (Hydroxymethylbilane synthase) hgpd (Glucose-phosphate dehydrogenase) Premier qpcr Kit (SYBR Green with low ROX) Premier qpcr Kit (SYBR Green with low ROX) Premier qpcr Kit (SYBR Green with low ROX) 3.% 5.5%.% Rn RFU (1^3) E=.% R =1. E=9.9% R = Rotor-Gene Q (QIAGEN, Germany) CFX-9 (Bio-Rad, USA) ABI 75 (Applied Biosystems, USA) Amplification of target DNA with various G+C contents using Premier qpcr Kit [SYBR Green with low ROX]. It was evaluated by real-time PCR using 1-fold serial diluted human genomic DNA (red: 5 ng/rxn, green: 5 ng/rxn, blue:.5 ng/rxn) with each Instruments. : Negative control. Get more information from
![95 Temperature, Celsius Comparison of Real-time PCR using Premier qpcr Kit [SYBR Green with low ROX] with other company s related products.](/docs-images/83/87271532/images/4-12.jpg "1-fold serial dilutions of the human genomic DNA (red: 5 ng/rxn, green: 5 ng/rxn, blue:.5 ng/rxn) from whole blood were analyzed using human HPRT-1 gene-specific primer set.")
4 Experimental Data (A) Rotor-GENE Q (QIAGEN) Nanohelix Company B Company E Cycling A. Green R=.9971 R^=.99 M= 3.99 B=.5 Efficiency= Cycling A. Green R=.9953 R^=.999 M=.1 B=.77 Efficiency= Cycling A. Green R=.991 R^=.9933 M= 3.7 B=.575 Efficiency= (B) CFX-9 (Bio-Rad) Nanohelix Company B Company E 1 1 E=9.5% R = E=9.1% R = E=.3% R =1. RFU (1^3) RFU (1^3) RFU (1^3) 1 3 s 1 3 s 1 3 s d(rfu)/dt 15 -d(rfu)/dt -d(rfu)/dt Temperature, Celsius Temperature, Celsius Temperature, Celsius Comparison of Real-time PCR using Premier qpcr Kit [SYBR Green with low ROX] with other company s related products. 1-fold serial dilutions of the human genomic DNA (red: 5 ng/rxn, green: 5 ng/rxn, blue:.5 ng/rxn) from whole blood were analyzed using human HPRT-1 gene-specific primer set. Reactions were run on a real-time PCR instrument, Rotor-GENE Q (A, QIAGEN) and CFX-9 (B, Bio-Rad). : Negative control. Get more information from
5 Ordering Information Product New Size Cat. no. Premier qpcr Kit [SYBR Green with low ROX] rxns PQL-S Premier qpcr Kit [SYBR Green with low ROX] 5 rxns PQL-S5 Premier qpcr Kit [SYBR Green with high ROX] rxns PQH-S Premier qpcr Kit [SYBR Green with high ROX] 5 rxns PQH-S5 Related Products Product Total RNA Purification Kit (Column type) Size Cat. no. PureHelix TM Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] 5 preps RPT5 PureHelix TM Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] 1 preps RPT1 PureHelix TM Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] preps RPT Easy cdna Synthesis Kit New HelixCript TM Easy cdna Synthesis Kit [All-in-One] 5 rxns ECDNA5 HelixCript TM Easy cdna Synthesis Kit [All-in-One] 1 rxns ECDNA1 qpcr Kit qpcr Kit [Intercalator type], (ROX solution provided) 1 rxns QPCR-S1 qpcr Kit [Intercalator type], (ROX solution provided) 5 rxns QPCR-S5 qpcr Kit [Probe type], (ROX solution provided) 1 rxns QPCR-P1 qpcr Kit [Probe type], (ROX solution provided) 5 rxns QPCR-P5 qrt-pcr Kit qrt-pcr Kit [Intercalator type], (ROX solution provided) 1 rxns QRT-S1 qrt-pcr Kit [Intercalator type], (ROX solution provided) 5 rxns QRT-S5 qrt-pcr Kit [Probe type], (ROX solution provided) 1 rxns QRT-P1 qrt-pcr Kit [Probe type], (ROX solution provided) 5 rxns QRT-P5 Order : info@nanohelix.net
6 NanoHelix Co., Ltd. Head Office (Tel : , Fax : ) 3F, Expo-Ro Beon-Gil 3, Yuseong-Gu, Daejeon 35-3, South Korea R&D Center (Tel : , Fax : ) #1, 17, Techno -Ro, Yuseong-Gu, Daejeon 35-5, South Korea International Distributor China : CLT Science & Technology Co., Ltd. Romania : Redox Lab Iran : LifeTebGen Co Turkey : Intron Healthcare Products company Italy : Cabru S.A.S Thailand : GIBTHAI CO., LTD Japan Malaysia : Cosmo Bio Co., Ltd. : NANO LIFE QUEST SDN. BHD.
PQL-S200 (200 rxns) Store -20. using the 10-fold serial-diluted human genomic DNA and a set of human gene-specific primer
CERTIFICATE OF ANALYSIS (1603-V01R02) RealHelix TM Premier q Kit [SYBR Green with low ROX] Kit contents RealHelix TM Premier q Kit [SYBR Green with low ROX] Cat. No. PQL-S200 (200 rxns) PQL-S500 (500 rxns)
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