Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions

Size: px
Start display at page:

Download "Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions"

Transcription

1 Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800) / Fax: (650)

2 INTRODUCTION Maxim s Plasmid DNA Isolation Column Kits provide a means to isolate plasmid DNA from bacteria. This kit provides all the necessary reagents and protocols to extract high quality plasmid DNA. This general purpose kit can be used to isolate any plasmid DNA but works most efficiently when the plasmid DNA is less than 20 Kb in size. The purified plasmid DNA can be used directly for DNA sequencing and other standard molecular biology techniques. Purification Using Maxim Spin Columns Maxim spin columns will fit into most standard microcentrifuge tubes. In addition, 2 ml collection tubes have been provided along with the kit for the loading and wash steps. DNA can be eluted into 1.5 ml microcentrifuge tubes, which are not provided. Purification procedures using spin columns are intended to extract nucleic acid with no sample to sample cross contamination and ensure the safety of the user while handling samples that are potentially infectious. Elution of Purified Nucleic Acids Purified DNA is eluted into distilled deionized water. To store purified DNA, elution into buffer comprised of 1 mm Tris-Cl, 0.05 mm EDTA, ph 8.3 is recommended. Store at -20 C. Nucleic acid stored in water may degrade due to acid hydrolysis. Determination of Concentration, Yield, and Purity DNA yield is determined from the concentration of eluted DNA in sterile double distilled water as measured by absorbance at 260 nm. Absorbance readings at 260nm should range from The sample should be diluted according to concentration. If the eluted sample contains ng of DNA/µL, should not be diluted with more than 4 volumes of buffer or water. Elution buffer or water should be used to dilute samples and to calibrate the spectrometer. Absorbance readings need to be measured at both 260 nm and 280 nm to determine the concentration of the DNA content of your product. To measure the concentration of DNA alone, use of a fluorimeter is recommended instead. To determine the purity of the sample, calculate the ratio between absorbance at 260 nm to the absorbance at 280 nm. Pure DNA will have an A260/A280 ration from DNA purified using Maxim plasmid DNA isolation column kits will be free from protein and other contaminants. Determination of DNA Size The size of the purified DNA product can be determined by gel electrophoresis. Run the purified product on a 1% agarose gel next to a DNA molecular weight marker. Load µg of DNA per well. Immerse the agarose gel in a 1x TBE electrophoresis buffer and run at 100 volts for 30 min. or until the loading dye front reaches 2/3 of the gel. 2

3 Preparation of bacteria culture 1. Inoculate a single bacterial colony from a fresh Luria-Bertani (LB) agar plate (containing antibiotic) to 1-10 ml LB broth (containing the same antibiotic). 2. Incubate overnight (12-16 hours) at 37 o C in a shaking incubator (250 rpm). For High Copy Number Plasmids - we do not recommend processing more than 5 ml of bacteria culture. If more than 5 ml of bacteria culture is used, the capacity of Maxim s spin column will be exceeded. The yield of plasmids DNA will not increase. For Low Copy Number Plasmids - the 10 ml bacteria culture is recommended for recovery of sufficient plasmid DNA. Proceeding more than 10 ml of bacteria culture will lead incomplete lysing the bacteria. It will increase the contaminants in the plasmid DNA. Handling of Maxim Spin Columns Use care when applying sample or solution to spin column. Try not to allow sample to touch the rim of the column. Change pipet tips between all material transfers. Use aerosol barrier pipet tips whenever possible. Do not let the pipet tip touch the Maxim spin column membrane. Briefly centrifuge 1.5mL microcentrifuge tubes after vortexing. Wear gloves when conducting purification procedure and avoid contact between sample and gloves. Changes gloves immediately should any contact occur. Microcentrifuge protocol Make sure to close the cap of the microcentrifuge tube over the spin column lid before centrifugation. Discard filtrate and the collection tube after centrifugation. Dispose of the filtrate appropriately as it may contain hazardous waste. Open only one microcentrifuge tube containing a spin column at a time, taking care to avoid aerosols. For increased efficiency, use a collection tube rack when conducting purification involving multiple samples. Centrifugation All centrifugation should be conducted at room temperature. The recommended speed for centrifugation is at least 10,000xg. A higher speed will not affect yields and is highly recommended, but lower speeds may be insufficient for transferring solutions completely through the membrane. 3

4 Plasmid DNA Isolation Column Kit Components Catalog No. SA SA Reaction No Cell Resuspension Solution 15 ml 30 ml Cell Lysis Solution 15 ml 30 ml Cell Neutralization Solution 20 ml 40 ml Washing Buffer 30 ml 60 ml Elution Buffer 10 ml 10 ml 1.5 ml microcentrifuge tube Spin Column Sample Source & Size Sample Source: Bacteria Culture Sample Size: 1-10 ml Storage Conditions For optimal results, Maxim spin columns should be stored under dry conditions at room temperature (15-25 C) for a maximum of one year. All of the solutions should be stored at C and are stable for up to 12 months. Required Equipment and Reagents 1.5mL microcentrifuge tubes Sterile DNase-free pipet tips with aerosol barrier Microcentrifuge with rotor for 2mL tubes Product Use Limitations Maxim Plasmid DNA Isolation Column Kits are intended for general nucleic acid purification purposes and not for any specific organism or for any specific clinical purpose. It is up to the user s discretion whether or not Maxim Plasmid DNA Isolation Column Kits are suitable for their specific experimental needs. Products should be handled with care. Please keep the NIH guidelines involving recombinant DNA experiments in mind when using Maxim Column Purification Kits. Product Warranty and Satisfaction Guarantee. Maxim guarantees the quality and performance of our products according to the standards detailed in our instruction manuals and publications. It is up to the user s discretion whether or not Maxim Plasmid DNA Isolation Column Kits are suitable for their specific experimental needs. If the Maxim Plasmid DNA Isolation Column Kit has been used properly yet fails to perform to your satisfaction, then Maxim will replace the kit free of charge or refund the purchase price for the item. To return or exchange an item, simply call our Technical Service Department. The Maxim technical service information is listed on the last page. Maxim reserves the right to modify or change any of its products to enhance performance. 4

5 Technical Assistance Maxim s Technical Service department is staffed by experienced scientists with expertise in various fields of immunology, pathology, and molecular biology, etc. If you have any questions of Maxim s products, please contact us at our technical hot line ext 14 or to 5

6 PROTOCOL 1. Harvest 5-10 ml bacterial culture by centrifugation for 5 minutes at 10,000 x g in a tabletop centrifuge. Pour off the supernatant and blot the inverted tube on a paper towel to remove excess media. 2. Add 250 µl of Cell Resuspension Solution and completely resuspend the bacterial pellet by vortexing or pipetting. Then, transfer the resuspended bacteria to a sterile 1.5 ml microcentrifuge tube. Note: It is very important to thoroughly resuspend the bacteria pellet. 3. Add 250 µl of Cell Lysis Solution and mix well by inverting several times until the lysate clears. Note: Do not vortex in this step. It is very important to observe the clearing of the lysate. 4. Add 350 µl of Cell Neutralization Solution and immediately mix well by inverting several times and Centrifuge for 5 minute at maximum speed (around 14,000 x g) at room temperature. Note: Do not vortex in this step. 5. Carefully transfer the clear lysate (approximately 850 µl) to the spin column in a 2.0 ml microcentrifuge tube provided and centrifuge for 1 minute at maximum speed. Note: Avoid disturbing or transferring any of the white precipitate with the supernatant. 6. Carefully transfer the spin column into a fresh 2.0 ml microcentrifuge tube provided. Add 500µL Washing Buffer into the spin column and centrifuge for 1 minute at maximum speed. 7. Carefully transfer the spin column into a new 1.5 ml microcentrifuge tube (not provided) and centrifuge for 1 minute at maximum speed. Note: This avoids any residual washing buffer. 8. Carefully transfer the spin column into a new 1.5 ml microcentrifuge tube (not provided). Add 100 µl Elution Buffer to the spin column, let the column stand for 1 minute, and centrifuge for 1 minute at maximum speed. Note: Eluted plasmid DNA is stable if kept at -20 C for long term storage or at 4-8 C for short term storage. 6

7 7

Presto Mini Plasmid Kit

Presto Mini Plasmid Kit Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured

More information

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only Plasmid Midiprep Plus Purification Kit Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Plus Purification Kit provides simple

More information

Plasmid DNA Extraction Miniprep Kit

Plasmid DNA Extraction Miniprep Kit BIO BASIC Plasmid DNA Extraction Miniprep Kit 9K-006-0009s (10 preps) 9K-006-0009 (100 preps) 9K-006-0010 (200 preps) For Research Use Only Index Plasmid DNA Extraction Miniprep Kit Introduction 3 Important

More information

Plasmid Maxiprep Plus Purification Kit

Plasmid Maxiprep Plus Purification Kit Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,

More information

Plasmid Maxiprep Plus Purification Kit. Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only

Plasmid Maxiprep Plus Purification Kit. Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,

More information

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Cat. # 9760 For Research Use TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 4 IV. Preparation

More information

Hurricane Miniprep Kit PROTOCOL

Hurricane Miniprep Kit PROTOCOL Hurricane Miniprep Kit PROTOCOL Description: The Hurricane Miniprep Kit is designed for purification of up to 25 ug of high purity plasmid DNA from a starting volume of 2-5 ml of bacterial culture. The

More information

Plasmid Midiprep Purification Kit

Plasmid Midiprep Purification Kit Plasmid Midiprep Purification Kit Cat. # : DP01MD/ DP01MD-100 Size : 20/100 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Purification Kit provides simple rapid protocol

More information

Plasmid DNA Extraction Midiprep Kit

Plasmid DNA Extraction Midiprep Kit BIO BASIC Plasmid DNA Extraction Midiprep Kit 9K-006-0027s (1 prep) 9K-006-0027 (25 preps) Toll Free 1-877-956-7778 ForOrder Research Use Only www.feldan.com 1 Index Plasmid DNA Extraction Midiprep Kit

More information

Mag-Bind Ultra-Pure Plasmid DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Ultra-Pure Plasmid DNA 96 Kit. M x 96 preps M x 96 preps M1258-00 1 x 96 preps M1258-01 4 x 96 preps December 2015 Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Plasmid Protocol with Lysate Clearance via Centrifugation...5

More information

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using

More information

Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510) Rev C

Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510) Rev C Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 4110111 Rev C Aurum Plasmid Mini Kit Instruction Manual For technical service, call your local Bio-Rad

More information

UltraClean Midi Plasmid Prep Kit

UltraClean Midi Plasmid Prep Kit UltraClean Midi Plasmid Prep Kit Catalog No. Quantity 12700-20 20 Preps Instruction Manual Please recycle Version: 05232014 1 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4

More information

BACMAX DNA Purification Kit

BACMAX DNA Purification Kit Cat. No. BMAX044 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 212 10/2012 1 EPILIT212 Rev. A

More information

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only.

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only. Instruction Manual Ver. 05.11.17 For Research Use Only I-Blue Midi Plasmid Kit & I-Blue Midi Plasmid Kit (Endotoxin Free) IB47180, IB47190 (2 Preparation Sample Kit) IB47181, IB47191 (25 Preparation Kit)

More information

Product # Specifications. Kit Specifications Column Binding Capacity 25 µg

Product # Specifications. Kit Specifications Column Binding Capacity 25 µg 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18000 Product Insert The BAC DNA

More information

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind PX Blood RNA 96 Kit M7763-00 1 x 96 preps M7763-01 4 x 96 preps June 2018 Mag-Bind PX Blood RNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Aurum Plasmid Mini Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510)

Aurum Plasmid Mini Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510) Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 Aurum Plasmid Mini Kit Instruction Manual For technical service, call your local Bio-Rad office, or

More information

Presto 96 Well gdna Bacteria Kit

Presto 96 Well gdna Bacteria Kit Presto 96 Well gdna Bacteria Kit 96GBB02 (2 x 96 well plates/kit) 96GBB04 (4 x 96 well plates/kit) 96GBB10 (10 x 96 well plates/kit) Instruction Manual Ver. 05.04.17 For Research Use Only Advantages Sample:

More information

Specifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg

Specifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18050 Product Insert The BAC DNA

More information

FosmidMAX DNA Purification Kit

FosmidMAX DNA Purification Kit Cat. No. FMAX046 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 204 10/2012 1 EPILIT204 Rev. A

More information

TIANpure Mini Plasmid Kit II

TIANpure Mini Plasmid Kit II TIANpure Mini Plasmid Kit II For purification of molecular biology grade DNA www.tiangen.com/en DP140918 Kit Contents Storage TIANpure Mini Plasmid Kit II Contents RNase A (10 mg/ml) Buffer BL Buffer P1

More information

Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free)

Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free) Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free) PM002, PME02 (2 Preparation Sample Kit) PM010, PME10 (10 Preparation Kit) PM025, PME25 (25 Preparation Kit) Instruction Manual Ver.

More information

TIANpure Mini Plasmid Kit

TIANpure Mini Plasmid Kit TIANpure Mini Plasmid Kit For purification of molecular biology grade DNA www.tiangen.com PP080822 TIANpure Mini Plasmid Kit Kit Contents Storage Contents RNaseA (10 mg/ml) Buffer BL Buffer P1 Buffer P2

More information

Bacteria Genomic DNA Purification Kit

Bacteria Genomic DNA Purification Kit Bacteria Genomic DNA Purification Kit Cat #:DP025/ DP025-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Bacteria Genomic DNA Purification Kit provides a rapid, simple, and

More information

RayBio Genomic DNA Magnetic Beads Kit

RayBio Genomic DNA Magnetic Beads Kit RayBio Genomic DNA Magnetic Beads Kit Catalog #: 801-112 User Manual Last revised January 4 th, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100

More information

QIAfilter Plasmid Midi Kit (Cat #: 12243)

QIAfilter Plasmid Midi Kit (Cat #: 12243) QIAfilter Plasmid Midi Kit (Cat #: 12243) Things to do before starting Add the provided RNase A solution to Buffer P1 before use. Use one vial of RNase A (centrifuge briefly before use) per bottle of Buffer

More information

Kits for isolation of plasmid DNA in medium scale

Kits for isolation of plasmid DNA in medium scale PLASMID MIDI KIT PLASMID MIDI ENDOTOXIN-FREE Cat. No. EM16 / EM17 Version: 1.2014 Kits for isolation of plasmid DNA in medium scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MIDI KIT PLASMID

More information

EasyPrep TM Plasmid Maxiprep Manual

EasyPrep TM Plasmid Maxiprep Manual EasyPrep TM Plasmid Maxiprep Manual Catalog#: PD05-01, PD05-02 PD05-11, PD05-12 Bioland Scientific LLC 14925 Paramount Blvd., Suite C Paramount, CA 90723 USA Tel: (877) 603-8882 Fax: (562) 733-6008 Email:

More information

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology Note: for laboratory research use only RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Cat. #: RP1202 (50preps) Signalway Biotechnology I. Kit Content, Storage Condition and Stability Content

More information

Plasmid DNA 96-Well Kit

Plasmid DNA 96-Well Kit Cat. no. EM21 Version: 1.2017 Plasmid DNA 96-Well Kit EXTRACTME is a registered trademark of BLIRT S.A. www.blirt.eu Cat. no. EM21 I. INTENDED USE The EXTRACTME PLASMID DNA 96-WELL KIT is designed for

More information

DNA Purification Magnetic Beads

DNA Purification Magnetic Beads DNA Purification Magnetic Beads Catalog #: 801-109 User Manual Last revised December 17 th, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector Page 1 of 5 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector 1. Digest 1 µg of pbluescript with Eco RI 2. Following digestion, add 0.1 volumes of 3M sodium acetate (ph

More information

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps May 2013

More information

TIANpure Midi Plasmid Kit

TIANpure Midi Plasmid Kit TIANpure Midi Plasmid Kit For purification of molecular biology grade DNA www.tiangen.com Kit Contents Storage TIANpure Midi Plasmid Kit Contents RNaseA (10 mg/ml) Buffer BL Buffer P1 Buffer P2 Buffer

More information

EndoFree Maxi Plasmid Kit

EndoFree Maxi Plasmid Kit EndoFree Maxi Plasmid Kit For purification of ultrapure plasmid DNA with high yields www.tiangen.com/en DP121221 EndoFree Maxi Plasmid Kit Kit Contents Storage (Spin Column) Cat.no. DP117 Contents RNase

More information

Plus Blood Genomic DNA Purification Kit

Plus Blood Genomic DNA Purification Kit Plus Blood Genomic DNA Purification Kit Cat #:DP023P/ DP023P-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Plus Blood Genomic DNA Purification Kit provides a rapid, simple

More information

DNA miniprep by Alkaline Lysis (activity)

DNA miniprep by Alkaline Lysis (activity) DNA miniprep by Alkaline Lysis (activity) Contents 1 Alkaline Lysis 2 Exercise 1: Plasmid DNA Mini-Prep by Alkaline Lysis 3 Identification of Plasmid DNA 4 Exercise 2: Restriction Digestion Identification

More information

Presto 96 Well DNA Bacteria Advanced Kit

Presto 96 Well DNA Bacteria Advanced Kit Instruction Manual Ver. 07.04.17 For Research Use Only Presto 96 Well DNA Bacteria Advanced Kit 96GBBA02 (2 x 96 well plates/kit) 96GBBA04 (4 x 96 well plates/kit) 96GBBA10 (10 x 96 well plates/kit) Advantages

More information

EasyPrep TM EndoFree Plasmid Maxiprep Purification Protocol

EasyPrep TM EndoFree Plasmid Maxiprep Purification Protocol Table of Contents EasyPrep TM EndoFree Plasmid Maxiprep Purification Protocol Introduction... 3 Important Notes.... 3 Before Starting... 4 PD05-21, PD05-22 PD05-31, PD05-32 Kit Contents... 5 EndoFree Plasmid

More information

Geneaid DNA Isolation Kit (Yeast)

Geneaid DNA Isolation Kit (Yeast) Geneaid DNA Isolation Kit (Yeast) GEY100, GEY300 Advantages Sample: up to 2 10 8 yeast and other fungus species Yield: high yield, high quality DNA (A260/A280 = 1.8-2.0) Format: scalable DNA precipitation

More information

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps November

More information

Kit for isolation of plasmid DNA

Kit for isolation of plasmid DNA Cat. No. EM01 Version: 1.2013 Kit for isolation of plasmid DNA EXTRACTME is a registered trademark of BLIRT S.A. www.dnagdansk.com Cat. No. EM01 I. INTENDED USE The EXTRACTME PLASMID DNA kit is designed

More information

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps E.Z.N.A. FFPE RNA Kit R6954-00 5 preps R6954-01 50 preps R6954-02 200 preps January 2015 E.Z.N.A. FFPE RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE Introduction The GF-1 PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from 100bp to 20kb. The GF-1 PCR Clean Up Kit contains special buffers to provide the correct salt concentration

More information

Micro-Elute DNA Clean/Extraction Kit

Micro-Elute DNA Clean/Extraction Kit Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/Gel Extraction/DNA Clean-Up /Concentration Kit) Cat. # : DP034ME/ DP034ME-300 Size : 50/300 Reactions Store at RT For research use only 1 Description:

More information

Maximum Yield Mini System. Protocol Book YPD100 // YPD300. Ver

Maximum Yield Mini System. Protocol Book YPD100 // YPD300. Ver HiYield Plasmid Kit Mini Maximum Yield Mini System Protocol Book YPD100 // YPD300 Ver. 2017-1 Precautions I) Handling Requirements Do not use a kit after its expiration date has passed. Some reagents

More information

Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100

Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 Product

More information

E.Z.N.A. Yeast RNA Kit. R preps R preps

E.Z.N.A. Yeast RNA Kit. R preps R preps R6870-00 5 preps R6870-01 50 preps September 2017 Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4 Preparing Reagents...5 Yeast RNA Protocol...6

More information

Introduction. Principle

Introduction. Principle Contents Introduction............................................................ 2 Principle.............................................................. 2 Storage and Stability.....................................................

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

MD60002 MD MD62002

MD60002 MD MD62002 MagSi-DNA saliva Art.No. MD60002 MD61002 - MD62002 Product Manual Version 1.0 22/01/2015 Table of Contents 1. General Information...3 1.1 Intended Use...3 1.2 Kit specifications...3 1.3 Basic principle...3

More information

StrataPrep Plasmid Miniprep Kit

StrataPrep Plasmid Miniprep Kit StrataPrep Plasmid Miniprep Kit INSTRUCTION MANUAL Catalog #400761 and #400763 Revision A For In Vitro Use Only 400761-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information

Lysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1

Lysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Kit (Slurry Format) Product # 48800 Product

More information

mdi Quanta Giga Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Quanta Giga Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Quanta Giga Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial +91-171-2699221,

More information

Presto Food DNA Extraction Kit

Presto Food DNA Extraction Kit Instruction Manual Ver. 06.28.17 For Research Use Only Presto Food DNA Extraction Kit Advantages FGD004 (4 Preparation Sample Kit) FGD100 (100 Preparation Kit) FGD300 (300 Preparation Kit) Sample: 200

More information

Biospin Plasmid DNA Maxi Extraction Kit

Biospin Plasmid DNA Maxi Extraction Kit Kit Components Biospin Plasmid DNA Maxi Extraction Kit Cat# BSC01S1C BSC01M1C Components 10Tests 25Tests Balance Buffer 20ml 50ml Resuspension Buffer 100ml 250ml Lysis Buffer 100ml 250ml Neutralization

More information

E.Z.N.A. M13 DNA Mini Kit

E.Z.N.A. M13 DNA Mini Kit E.Z.N.A. M13 DNA Mini Kit D6900-00 5 preps D6900-01 50 preps EZ-96 M13 DNA Kit D1900-00 2 preps D1900-01 6 preps June 2013 E.Z.N.A. M13 DNA Mini Kit E-Z 96 M13 DNA Kit Table of Contents Introduction and

More information

mdi Medi-R Total RNA PLUS Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies

mdi Medi-R Total RNA PLUS Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies Medi-R Total RNA PLUS Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21,

More information

Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation

Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation BDtract TM Genomic DNA Isolation Kit Instruction Manual Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800)

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: 100 Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

Presto Mini gdna Bacteria Kit

Presto Mini gdna Bacteria Kit Instruction Manual Ver. 02.10.17 For Research Use Only Presto Mini gdna Bacteria Kit Advantages GBB004 (4 Preparation Sample Kit) GBB100/101 (100 Preparation Kit) GBB300/301 (300 Preparation Kit) Sample:

More information

Kits for isolation of plasmid DNA in large scale

Kits for isolation of plasmid DNA in large scale PLASMID MAXI KIT PLASMID MAXI ENDOTOXIN-FREE Cat. No. EM18 / EM19 Version: 1.2014 Kits for isolation of plasmid DNA in large scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MAXI KIT PLASMID

More information

Gel/PCR Extraction Kit

Gel/PCR Extraction Kit Gel/PCR Extraction Kit Item No: EX-GP200 (200rxns) Content Content Binding Buffer BD Wash Buffer PE Elution Buffer (10 mm Tris-HCl, ph 8.5) Spin Columns EX-GP200 80 ml 20 mlx3 10 ml 200 each Description

More information

Soil DNA Extraction Kit

Soil DNA Extraction Kit Instruction Manual Ver. 09.23.16 For Research Use Only Soil DNA Extraction Kit Advantages IB47800 (4 Preparation Sample Kit) IB47801 (50 Preparation Kit) IB47802 (100 Preparation Kit) Sample: 250-500 mg

More information

mdi Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:

More information

BiOstic Bacteremia DNA Isolation Kit Sample Catalog No S (For use with cultured blood for the enrichment of bacteria)

BiOstic Bacteremia DNA Isolation Kit Sample Catalog No S (For use with cultured blood for the enrichment of bacteria) BiOstic Bacteremia DNA Isolation Kit Sample Catalog No. 12240-S (For use with cultured blood for the enrichment of bacteria) Information for Ordering Product Catalog No. Quantity 12240-50 50 preps Instruction

More information

mdi Endotoxin Free Quanta Mega Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Endotoxin Free Quanta Mega Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Endotoxin Free Quanta Mega Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:

More information

AuPreP Plasmid Midi Kit Cat. #: PMD12-143LT (25 preps/kit) PMD12-145LT (50 preps/kit)

AuPreP Plasmid Midi Kit Cat. #: PMD12-143LT (25 preps/kit) PMD12-145LT (50 preps/kit) AuPreP Plasmid Midi Kit Cat. #: PMD12-143LT (25 preps/kit) PMD12-145LT (50 preps/kit) AuPreP Plasmid Midi Kit is designed for rapid isolation of plasmid DNA of superior quality from an average of 25-100

More information

mdi Quanta Midi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Quanta Midi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Quanta Midi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial +91-171-2699221,

More information

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps E.Z.N.A. HP Viral RNA/DNA Kit R6873-00 5 preps R6873-01 50 preps August 2013 E.Z.N.A. HP Viral RNA/DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021/ DP Size:50/150 reactions Store at RT For research use only

Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021/ DP Size:50/150 reactions Store at RT For research use only Tissue & Cell Genomic DNA Purification Kit Cat. #:DP021/ DP021-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Tissue & Cell Genomic DNA Purification Kit provides a rapid,

More information

FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit. User Manual

FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit. User Manual TM FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit User Manual Cat. No.: FABGK 003 (10 Preps) FABGK 003-1 (24 Preps) For Research Use Only v.1005 Introduction TM FavorPrep Genomic DNA Extraction

More information

Presto Soil DNA Extraction Kit

Presto Soil DNA Extraction Kit Instruction Manual Ver. 02.23.17 For Research Use Only Presto Soil DNA Extraction Kit Advantages SLD004 (4 Preparation Sample Kit) SLD050 (50 Preparation Kit) SLD100 (100 Preparation Kit) Sample: 250-500

More information

mdi Endotoxin Free Quanta Maxi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Endotoxin Free Quanta Maxi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Endotoxin Free Quanta Maxi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:

More information

The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA.

The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA. INSTRUCTION MANUAL Zyppy Plasmid Midiprep Kit Catalog Nos. D4025 & D4026 (Patent Pending) Highlights The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA. Pellet-Free

More information

Presto Stool DNA Extraction Kit

Presto Stool DNA Extraction Kit Instruction Manual Ver. 10.21.17 For Research Use Only Presto Stool DNA Extraction Kit Advantages STLD004 (4 Preparation Sample Kit) STLD050 (50 Preparation Kit) STLD100 (100 Preparation Kit) Sample: 180-200

More information

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps E.Z.N.A. Yeast Plasmid Mini Kit D3376-00 5 preps D3376-01 50 preps November 2015 E.Z.N.A. Yeast Plasmid Mini Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

TIANgel Mini DNA Purification Kit

TIANgel Mini DNA Purification Kit TIANgel Mini DNA Purification Kit For DNA purification from agarose and polyacrylamide gels www.tiangen.com/en DP130419 TIANgel Mini DNA Purification Kit Kit Contents (Spin column) Cat. no. DP208 Contents

More information

Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only

Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only Sample : up to 300 µl of whole blood, up to 200 µl of frozen blood, up to 200 µl of buffy coat, cultured animal cells (up to 1 x 107), 9

More information

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only.

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only. Note: for laboratory research use only. Endotoxin-free Plasmid DNA Mini-preparation Kit (Spin-column) Cat. # DP2601 (20 preps) DP2602 (50 preps) BioTeke Corporation 1 I. Kit Content Storage and Stability

More information

Catalog No. SA-40004: 50 preparation SA-40003: 100 preparation

Catalog No. SA-40004: 50 preparation SA-40003: 100 preparation BDtract TM RNA/DNA Isolation Kit Instruction Manual Catalog No. SA-40004: 50 preparation SA-40003: 100 preparation Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800)

More information

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella foodproof StarPrep Two Kit Order No. S 400 08 Quick Reference Procedure for Legionella Version 1, November 2017 A. Kit Contents / Storage and Stability Product Content Storage S 400 08 1 container with

More information

Plus DNA Clean/Extraction Kit

Plus DNA Clean/Extraction Kit Plus DNA Clean/Extraction Kit Cat. # : DP034P/ DP034P-300 Size : 50/300 Reactions Store at RT For research use only 1 Description: The Plus DNA Clean/Extraction Kit is designed to extract DNA fragments

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: 100 Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

RNAprotect Bacteria Reagent Handbook

RNAprotect Bacteria Reagent Handbook January 2015 RNAprotect Bacteria Reagent Handbook RNAprotect Bacteria Reagent For in vivo stabilization of total RNA in bacteria RNeasy Protect Bacteria Mini Kit RNeasy Protect Bacteria Midi Kit For in

More information

VDL101.3 CLONING TRANSGENE INTO pad5f35

VDL101.3 CLONING TRANSGENE INTO pad5f35 Purpose 1.1. The purpose of this protocol is to transfer a transgene from the pshuttlex plasmid to pad5/f35. 1.2. The starting material is 10 μg plasmid DNA. 1.3. This procedure is routinely performed

More information

(Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded. tissue sections. Instruction for Use. For Research Use Only

(Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded. tissue sections. Instruction for Use. For Research Use Only AmoyDx FFPE DNA/RNA Kit (Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded tissue sections Instruction for Use For Research Use Only Instruction Version: B1.0 Revision

More information

Spin Micro DNA Extraction Kit

Spin Micro DNA Extraction Kit Spin Micro DNA Extraction Kit 2-020 20 extractions 18-25 C 1. Lysis Buffer (brown cap) 2x 2 ml R 36 2. Binding Buffer (red cap) 3x 2 ml R 11/36/67 3. Protease (orange cap) lyophilized R 36/37/38 Add 250

More information

For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues

For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues 1. TIANamp DNA/RNA Isolation Kit For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues www.tiangen.com/en RP090603 TIANamp DNA/RNA Isolation Kit Kit Contents

More information

E.Z.N.A. Bacterial RNA Kit. R preps R preps

E.Z.N.A. Bacterial RNA Kit. R preps R preps E.Z.N.A. Bacterial RNA Kit R6950-00 5 preps R6950-01 50 preps July 2017 E.Z.N.A. Bacterial RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4

More information

E.Z.N.A. Tissue RNA Kit. R preps R preps

E.Z.N.A. Tissue RNA Kit. R preps R preps E.Z.N.A. Tissue RNA Kit R6688-00 5 preps R6688-01 50 preps May 2015 E.Z.N.A. Tissue RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Important Notes...4 Homogenization

More information

Storage and Stability

Storage and Stability Contents Introduction....................................................... 2 Kit Contents...................................................... 3 Before Starting....................................................

More information

PureLink HiPure Plasmid DNA Purification Kits

PureLink HiPure Plasmid DNA Purification Kits USER GUIDE PureLink HiPure Plasmid DNA Purification Kits For mini, midi, and maxi preparation of plasmid DNA Catalog Numbers Revision B.0 Publication Number MAN0000486 K2100-02, K2100-03, K2100-04, K2100-05,

More information

InterLab Study: Plasmid amplification

InterLab Study: Plasmid amplification igem TU/e 2015 Biomedical Engineering Eindhoven University of Technology Room: Ceres 0.04 Den Dolech 2, 5612 AZ Eindhoven The Netherlands Tel. no. +31 50 247 55 59 2015.igem.org/Team:TU_Eindhoven InterLab

More information

E.Z.N.A. mirna Kit. R preps R preps R preps

E.Z.N.A. mirna Kit. R preps R preps R preps E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

GET Plasmid DNA 96 Well

GET Plasmid DNA 96 Well 187PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name GET Plasmid DNA 96 Well For High Yield & Quality Plasmid DNA Extraction (Cat. # 786

More information