A Level Biology B (Advancing Biology) H422/03 Practical skills in biology

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1 A Level Biology B (Advancing Biology) H422/03 Practical skills in biology Practice paper Set 1 Time allowed: 1 hour 30 minutes You must have: the Insert (inserted) You may use: a scientific calculator a ruler (cm/mm) First name Last name Centre number Candidate number INSTRUCTIONS Use black ink. You may use an HB pencil for graphs and diagrams. Complete the boxes above with your name, centre number and candidate number. Answer all the questions. Write your answer to each question in the space provided. If additional space is required, use the lined page(s) at the end of this booklet. The question number(s) must be clearly shown. Do not write in the barcodes. INFORMATION The total mark for this paper is 60. The marks for each question are shown in brackets [ ]. Quality of extended responses will be assessed in questions marked with an asterisk (*). This document consists of 20 pages. OCR 2016 PRACTICE PAPER OCR is an exempt Charity H42203/12 Turn over

2 2 Answer all the questions. 1 (a) A farmer with an orchard produces cider using the juice from the apples. An advisor from the Government visits the farm and suggests that the farmer could graze sheep on the grass in the orchard to qualify for additional grant payments. The advisor says that the productivity figures for the sheep will not be as good as the figures for the apples. Table 1.1 shows some of the energy content and transfer efficiencies for the food chain: Grass Sheep Human Energy Content / MJ ha -1 year -1 Total incoming solar energy Energy provided by photosynthetic wavelengths Total primary production Above ground Below ground Total secondary production Table 1.1 (i) Calculate the percentage efficiency of the conversion of photosynthetic wavelengths into above ground primary production. Show your working. Give your answer to two decimal places. Answer... % [2] (ii) The advisor calculates the efficiency of energy conversion for grass (primary production) and sheep (secondary production) in the food chain. Using your knowledge of food chains, explain why the percentage efficiency figures for primary (0.50%) and secondary (0.002%) production are so different.... [2] OCR 2016 PRACTICE PAPER H422/03

3 3 (b) The farmer produces prize-winning cider which is bottled on the farm. To make the perfect cider, it must contain just the right volume of carbon dioxide (CO 2 ) gas to make it fizzy. Live yeast remaining in the sealed bottles of cider produce this CO 2. Name the process by which live yeast cells make CO 2 in a sealed bottle..... [1] (c) A haemocytometer is used to estimate the population of live yeast in a sample of undiluted cider. The stain methylene blue is added to the sample. Only live yeast cells will take up this stain. Fig 1.1 shows the haemocytometer chamber containing the cider sample when viewed under a light microscope. Fig. 1.1 (i) The depth of the haemocytometer chamber, shown in Fig. 1.1, is 0.2 mm. Calculate the number of live yeast cells in 1 cm 3 of the cider sample. Show your working. Answer... [3] OCR 2016 PRACTICE PAPER H422/03 Turn over

4 4 (ii) At the time it is bottled the optimum number of live yeast cells is between to cells per cm 3 of cider. This ensures the finished cider will contain just the right volume of CO 2. Using this information and your answer to (c)(i) would this cider be ready for bottling? Justify your answer.... [1] (d) Flow cytometry is another technique that can be used for counting cells. Compare the suitability of both techniques for counting the number of yeast cells in this population [3] OCR 2016 PRACTICE PAPER H422/03

5 2 A horse being treated by a vet appears to have pneumonia caused by a bacterial infection. 5 Antibiotics will be used to treat the horse, but the vet must first find out which species of bacterium is causing the pneumonia. A sample of fluid containing the bacteria is taken from the horse and sent to a laboratory for analysis in a small lidded bottle. (a)* Write a method for safely obtaining a pure culture of the bacteria from the fluid sample. You should name any equipment needed and justify your choice of equipment. Your method must be based on the assumption that you are provided with school or college resources [9] OCR 2016 PRACTICE PAPER H422/03 Turn over

6 6 (b) The vet discovers that two different species of bacteria are present which may be causing the pneumonia. Once suitably prepared and stained using the Gram staining technique, a sample of the bacteria are observed using the high power lens of a light microscope. In one of the bacterial species, the cells are seen to be stained red/pink. (i) Why must a high power lens be used to view the bacteria?... [1] (ii) What does the red/pink colour of the stained bacteria suggest about the structure of the cells?... [1] (c) Fig. 2.1, on the Insert, is a photomicrograph of the bacterium, Klebsiella pneumoniae, the probable cause of the pneumonia. (i) The cell labelled A, in Fig. 2.1, is undergoing cell division by a process called binary fission. Suggest why these bacterial cells do not divide by mitosis....[1] (ii) The cell labelled B, in Fig. 2.1, has a width of 5 μm measured between X and Y. Calculate the magnification of the photomicrograph. Show your working. Give your answer to three significant figures. Answer X... [2] OCR 2016 PRACTICE PAPER H422/03

7 7 Fig. 2.2 shows the results of a test carried out using the Klebsiella pneumoniae bacteria cultured from the horse. The bacterial culture was spread on an agar plate and discs containing antibiotics A to D were placed on the agar as shown. The plate was then incubated overnight. Fig. 2.2 Table 2.1 shows information about the 4 antibiotics used in the test. Antibiotic Mode of Action Notes A Inhibits the synthesis of protein Family of over 20 antibiotics by bacteria, leading to cell death B Inhibits bacterial cell wall Most widely used antibiotics synthesis C Inhibits bacterial cell wall Resistance evolves rapidly synthesis D Interferes with bacterial DNA Common drug of last resort used replication and transcription with resistant strains Table 2.1 (d) The vet chooses to use antibiotic A. Using Fig 2.2 and Table 2.1, justify this decision [2] OCR 2016 PRACTICE PAPER H422/03 Turn over

8 3 (a) Fig. 3.1, on the Insert, is a colour enhanced light micrograph of mammalian pancreatic tissue. (i) Name the structure labelled Z in Fig [1] (ii) In the space below draw a labelled and annotated plan of the pancreatic tissue shown in Fig [3] (b) A student is asked to analyse some blood plasma from a Type 1 diabetic person for the presence of glucose. The analysis is carried out by heating a plasma sample with Benedict s solution in a boiling water bath and observing any colour change. A colorimeter is then used to obtain quantitative results. OCR 2016 PRACTICE PAPER H422/03

9 9 Table 3.1 shows the blood glucose concentrations recorded by the student over five days. Time when blood Day 1 Day 2 Day 3 Day 4 Day 5 glucose tested 07: : : : : : : : : Mean Table 3.1 (i) Complete Table 3.1, by calculating the mean for Day 4. [1] (ii) Calculating a mean blood glucose concentration to plan the correct doses of insulin for each day is inappropriate. Explain why....[2] (iii) The data for the morning of Day 4 appear to be different from the other days. Suggest two possible reasons why [1] (c) Apart from using Benedict s solution and a colorimeter, suggest two alternative quantitative methods for measuring the concentration of glucose in blood plasma [2] OCR 2016 PRACTICE PAPER H422/03 Turn over

10 10 4 Maize plants can be grown commercially in greenhouses. The greenhouses have lighting systems but are unheated. The lighting systems used are controlled electronically and will be turned off when temperatures inside the greenhouses fall below 10 o C. Fig. 4.1 shows a graph of the rate of photosynthesis of maize at different temperatures. Fig. 4.1 (a) (i) Indicate, with an arrow, a compensation point on this graph. The answer to this question must be written on Fig [1] (ii) Suggest why the control system for the lights is programmed to switch them off at low temperatures.... [1] (iii) Explain why it is necessary to recalibrate the control system regularly.... [1] OCR 2016 PRACTICE PAPER H422/03

11 11 (b) Some varieties of maize plants have smooth kernels (seeds), whereas others have wrinkled kernels. This is a genetic trait. Varieties with smooth kernels are rich in starch and useful for making flour. A farmer has been given some smooth seeds all of the same unknown genotype. The farmer carries out a cross-breeding experiment using these seeds and some known to be heterozygous for this trait. The results are shown in Table 4.1. F 1 phenotype Observed results Expected results Smooth 547 Wrinkled 185 Total 732 Table 4.1 The 2 statistic is calculated in the following way: ( observed expected) expected 2 2 (i) Calculate the value of 2 for the above data. Show your working. Answer... [2] (ii) Table 4.2 shows a critical values table. probability, p Degrees of freedom Table 4.2 Using your calculated value of 2 and Table 4.2 what conclusions should you make about the significance of the difference between the observed and expected results? OCR 2016 PRACTICE PAPER H422/03 Turn over

12 12 (iii)...[2] Describe the genotype of the seed the farmer was given. Give a reason for your answer.... [2] OCR 2016 PRACTICE PAPER H422/03

13 13 5 Fig. 5.1 shows VO 2 max for a number of different mammals. Fig. 5.1 (a) (i) Describe the trend in the data shown in Fig [1] (ii) One point in the data, shown in Fig. 5.1, does not fit the trend. Name the mammal. Mammal... The data for this mammal is accurate. Suggest one reason why the VO 2 max for this mammal does not fit the trend. [1] (iii) Suggest why a logarithmic scale has been used on the x-axis of the graph in Fig [1] OCR 2016 PRACTICE PAPER H422/03 Turn over

14 14 (b) Higher VO 2 max is associated with better race performance. An athlete has a VO 2 max of 38 cm 3 kg -1 min -1. The athlete s trainer suggests experimenting with carbohydrate-loading to find out if carbohydrate-loading improves VO 2 max. During the experiment, the athlete raced once a week and each time the VO 2 max was measured. For the first 8 weeks, the athlete ate a normal diet. For weeks 9 to 20, the athlete followed a carbohydrate-loading diet plan. The athlete s VO 2 max was measured every week during the investigation. The results are shown in Table 5.1. Week Treatment VO 2 max (cm 3 kg -1 min -1 ) 1 Normal diet Mean VO 2 max 39 (cm 3 kg -1 min -1 ) 9 Carbohydrate-loading Mean VO 2 max (cm 3 kg -1 min -1 ) 41 Table 5.1 (i) Plot a graph, on the grid provided, to show the mean values of the data shown in Table 5.1 in the most appropriate way. Add range bars to show the highest and lowest VO 2 max values in each of the two treatments. [4] OCR 2016 PRACTICE PAPER H422/03

15 15 OCR 2016 PRACTICE PAPER H422/03 Turn over

16 16 (ii)* If the results of the investigation show that the carbohydrate-loading improves VO 2 max the trainer would recommend it for all the athletes they coach. Evaluate the investigation the trainer carried out. Your account should include a consideration of: variables confidence in the data validity....[6] END OF QUESTION PAPER OCR 2016 PRACTICE PAPER H422/03

17 17 ADDITIONAL ANSWER SPACE If additional answer space is required, you should use the following lined page(s). The question number(s) must be clearly shown in the margin(s). OCR 2016 PRACTICE PAPER H420/03 Turn over

18 18 OCR 2016 PRACTICE PAPER H422/03

19 19 OCR 2016 PRACTICE PAPER H420/03 Turn over

20 20 Copyright Information: OCR is committed to seeking permission to reproduce all third-party content that it uses in its assessment materials. OCR has attempted to identify and contact all copyright holders whose work is used in this paper. To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced in the OCR Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download from our public website ( after the live examination series. If OCR has unwittingly failed to correctly acknowledge or clear any third-party content in this assessment material OCR will be happy to correct its mistake at the earliest possible opportunity. For queries or further information please contact the Copyright Team, First Floor, 9 Hills Road, Cambridge CB2 1GE. OCR is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge. OCR 2016 PRACTICE PAPER H422/03

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