USWBSI Barley CP Milestone Matrix Updated

Transcription

1 USWBSI Barley CP Milestone Matrix Updated RA: Variety Development and Host Resistance (VDHR) CP Objective 2: Map Novel QTL for resistance to FHB in barley BS, KS Dec 2013 Make initial crosses of donor sources to Quest BS, KS May 2014 Select best accession and backcross F 1 s to Quest BS, KS Dec 2014 Backcross BC 1 s to Quest F 1 lines BC 1 lines BC 2 lines BS, KS Jan 2015-Apr 2016 Advance BC 2 F 1 generation to BC 2 F 5 BC 2 F 5 lines BS, KS Dec 2017 Phenotype BC 2 F 5 and BC 2 F 5:6 generations and genotype BC 2 F 5 /advance pop to BC 2 F 5:6 Phenotype and genotype data on advanced backcross population/ BC2F5:6 lines BS, KS May 2018 Lines with best resistance submitted to breeders/final report and publication Resistant breeding lines/publication

2 CP Objective 3. Validate and fine map FHB resistance QTL GM Apr 2016 Fine Map the ch. 6H bin7 QTL Development of robust molecular markers specific to ch. 6H bin 7 QTL GM Apr 2016 Identify recombinants between FHB resistance and grain protein content Lines with resistance QTL and lower grain protein CP Objective 4. Develop new barley varieties with enhanced resistance to FHB and lower DON RH, KS Mar 2014 Advance more lines into AMBA Plant Scale Testing More recommended varieties with enhanced scab resistance RH, KS May - Jul 2014 Evaluate eight new lines with DON levels at 70% or less of Robust to AMBA pilot testing. Performance data on eight lines. RH, KS Mar 2015 Advance more lines into AMBA Plant Scale Testing More recommended varieties with enhanced scab resistance RH, KS May - Jul 2015 Evaluate eight new lines with DON levels at 70% or less of Robust to AMBA pilot testing. Performance data on eight lines. KS Mar 2015 Entry of lines from facultative six-row program Recommended winter varieties with enhanced scab resistance KS, AL May-July 2014 Yield trials for Cycle 3 GS lines in MN Performance of cycle 3 lines KS, AL Oct 2014 GS predictions and Cycle 5 parent selection Genomic predictions for FHB and other traits for 1,536 lines

3 KS, AL May-July 2015 Yield trials for Cycle 4 GS lines in MN Performance of cycle 4 lines KS, AL Oct 2015 GS predictions and Cycle 6 parent selection Genomic predictions for FHB and other traits for 1,536 lines KS, AL Dec 2015 Evaluate data and realized gains to improve methods of prediction accuracy New methods to design training panels to improve prediction accuracy May 2014, Cross FHB/DON resistant lines with elite hulled, hulles and malt barley germplasm. 100 new crosses made annually; F 1 lines used for top crossing and advanced in field during subsequent seasons. May 2014, Genetic markers identified and validated in winter barley for FHB resistance QTL, other important diseases, yield and quality. Markers amenable to VT winter barley program identified May 2014, Parental lines genotyped and populations enriched for known FHB resistance QTL and other desirable traits. Pure lines derived from FHB breeding populations selected and advanced. Selected pure lines grown and evaluated in replicated yield and FHB trials. Pure lines derived from FHB breeding populations selected and advanced. Aug 2014, Aug 2015 Evaluate elite germplasm from VT state variety trial for FHB/DON resistance. FHB and DON data for all cultivars and elite lines provided to growers via extension publications and website. RB et al. Oct 2014 Evaluate best FHB material from Midwest and Canadian breeding programs in NABSEN NABSEN Report- Best FHB/DON lines identified for 2014 Fall crossing block

4 RB et al. Oct 2015 Evaluate best FHB material from Midwest and Canadian breeding programs in NABSEN NABSEN Report- Best FHB/DON lines identified for 2015 Fall crossing block RB Oct 2014, Oct 2014 Evaluate material from western U.S. breeding programs in Fargo and Langdon, ND. Identify Western U.S. adapted varieties/germplasm with native FHB and DON resistance. RA: Gene Discovery and Engineering Resistance (GDER) CP Objective 6: Evaluating promising transgenes in adapted genetic backgrounds in regional nurseries LD May 2014 Establish Langdon Transgenic FHB nursery and continue backcrossing selected lines LD Sept-Dec 2014 Evaluate and analyze FHB performance data from 2014 transgenic field trials LD Sept Mar 2015 Continue backcrossing for homozygous line development and increase seed Transgenic lines in elite backgrounds LD Establish Langdon Transgenic FHB nursery and continue backcrossing selected lines LD Sept-Dec 2015 Evaluate and analyze FHB performance data from 2015 transgenic field trials

5 LD Sept Mar 2016 GM May Apr 2015 Continue backcrossing for homozygous line development and increase seed Asses DON and DON3G concentration in transgenic barley lines Transgenic lines in elite backgrounds Data on DON and DON3G in HvUGT transgenic lines GM Apr 2016 Asses scab resistance in HvUGT transgenic barley lines Possible scab resistant barley lines RA: Pathogen Biology and Genetics (PBG) CP Objective 7: Investigate host genotype x pathogen interaction for infection, spread, disease development, and accumulation of DON. SA, EB Aug 2014, Aug 2015 Determine impact of chemotype on FHB and DON accumulation in susceptible (Robust) and resistant (Quest) varieties Understanding of host-pathogen interactions resulting in FHB severity and DON accumulation SA, EB Dec 2015, Dec 2016 Characterize chemotypes and genotypes of the South Dakota F. graminearum population Better understanding of the virulence of South Dakota F. graminearum population and possible impact on deployed resistance SA, EB Dec 2016 Determine fungicide sensitivity of SD F. graminearum isolates Fungicide sensitivity data FT June 2014 Laser Capture Microdissection of infected cells where fungi is in the process of crossing through plasmadesmota Tissue ready for RNAseq

6 FT Dec 2014 RNA sequencing performed on three independent RNA pools Genes upregulated and important for fungal infection processes identified FT Apr F. graminearum gene knockouts developed List of potential genes important for fungal pathogenicity FT April 2016 F. graminearum gene silencing constructs and transgenics developed Validation of candidate genes required for cell to cell movement