FastPrep System. MP Biomedicals. Life Science. A Complete Solution for your Sample Preparation

Transcription

1 MP Biomedicals Life Science FastPrep System A Complete Solution for your Sample Preparation Homogenizers Adapters Lysing Matrix DNA/RNA & Protein Purification Kits

2 Open a new Dimension in your Lab! Increase your productivity, throughput and performance in DNA, RNA & Protein Extraction and Purification. MP Biomedicals provides a complete range of products for all steps of sample preparation. From lysis, extraction through purification, as complex as the sample can be, we offer you the best solution to achieve best results for your work. MP Biomedicals has a long history in producing solutions for sample preparation and has developed with its best experience a wide range of instruments, lysing matrices and purification kits. - The FastPrep System - Whatever you need, we have the solution. 2

3 FastPrep System Workflow Select the Best Homogenization and Extraction Solution for your Application. Homogenization Homogenizers...4 to 7 FastPrep-24 5G FastPrep-96 SuperFastPrep-2 Adapters...8 & 9 Metal Cryogenic High throughput Large Sample volume Lysing Matrix...10 & 11 2 ml 4,5 ml 15 ml 50 ml 96 well plates Extraction DNA, RNA & Protein Isolation...12 & 15 DNA RNA Protein Gel analysis Purify Manual Extraction MPure-12 TM Automated Extraction 3

4 Homogenizers Often imitated. Never duplicated. Most Powerful - Highest speed available improves the quality, yield and purity of the resultant analyte Proven Performance - Delivers the most DNA, RNA and from any sample type including the most resistant samples in 40 seconds or less easily and conveniently Exclusive Software & Touchscreen - Intuitive, easy touch screen programming or choose from 70 recommended pre-programmed optimized application protocols Unmatched Flexibility - Industries largest variety of interchangeable sample holders, processing samples from 2-50 ml, in ambient or cryogenic temperatures Highest Yields - Proven track record to obtain the highest quantities and purity of biologically active DNA, RNA and compared to other methods Cat. No Description FastPrep-24 TM 5G Instrument The FastPrep-24 TM 5G Instrument is the latest version of the well-known FastPrep system. This versatile sample disruption device gives the ultimate in speed and performance for the lysis of biological samples. A completely self-contained system, the FastPrep-24 TM 5G Instrument eliminates the risk of cross-contamination and time-consuming clean-up associated with manual lysis method. Samples and buffers are simply added to a Lysing Matrix tube containing specialized Lysing Matrix beads. The ergonomic design of the instrument ensures an easy loading of the sample tubes that remains securely sealed during the processing. The homogenization speed and duration times are manually programmed using a touchscreen visual display. After setting your speed and time with the touch of a button, just push «run», and in less than a minute your samples are completely lysed! Typical FastPrep-24 TM 5G Procedure Cells and lysing buffer 1. Prepare lysis tube 40 seconds 2. Process with FastPrep-24 5G Instrument 1 minute 3. Centrifuge to pellet debris DNA RNA Protein Gel analysis Purify 4. Extract & Purify 4

5 Next Generation Sample Disruption System! The FastPrep-24 5G product line offers a complete solution to serve researcher s entire workflow from lysis to purification of DNA, RNA and proteins. The unique tridimensional motion of the instrument, the widest variety of Lysing Matrix tubes, and complete FastPrep Purification Kits make the FastPrep-24 5G the most advanced Sample Preparation System available in the market. Already 10,000 worldwide researchers acquired a FastPrep in their lab! A proven technology for several research areas: Environmental: microbial ecology, water research, agriculture Plant: molecular plant pathology, genotyping, GMO DNA detection, plant biotechnology Food: pathogen detection, food authentication, food research Forensics: hair analysis, toxicology, cause of death Health: cancer research, genetic research, parasitology and virology Upgraded Features Increased Power Settings to 10 m/sec. Benefits Highest Power Available Fastest Processing Times Lyse Tougher Samples Time: Seconds Cycles: 1-9 Cycle Pause: seconds Touchscreen User Interface Less User Intervention True Walk-Away Operation Intuitive and Easy to Operate >70 Pre-defined settings Quicker Optimizations, Saves Time Manual Program-N-Store (12 sets) Export Data Function USB Port Sound Interface Strobe Effect Saves Time and Knowledge Archive Assays and Results Easy Software Upgrades Audible Program Run Status Alerts Visual Optimization Tool 5

6 Homogenizers FastPrep-96 The Ultimate in High-Throughput Sample Preparation High Throughput - Process up to 192 samples simultaneously in 2 x 96 deep well plates Fast Processing Speed Oscillations/min and 1.5 inch stroke highest available Excellent Reproducibility - Consistent lysis results well to well Exceptional Versatility - Interchangeable sample holders available for 2 x 96 deep well plates, 96 x 2 ml tubes, 48 x 4.5 ml tubes, 24 x 15 ml tubes, 8 x 50 ml tubes, and 2 x 250 ml flasks True Linear Motion - Eliminates the need to re-orient plates mid-cycle Cat. No Description FastPrep-96 Instrument (includes 2 x 96 well plate sample holder) The FastPrep-96 TM system delivers high-throughput sample homogenization, grinding and lysis with the highest efficiency, quality and reproducibility. Perform your protein and small molecule extractions from the most difficult, dirty, tough, large or tiny samples. With the highest power settings available, FastPrep-96 utilizes high-speed linear motion to disrupt any tissues or cells thoroughly through the simultaneous beating of specialized lysing matrix particles. FastPrep-96 offers a wide range of sample adapters and a simple, accurate, closed loop control of lysing power and speed. All this and more make the new FastPrep-96 the perfect solution for all of your high volume sample preparation needs. Typical FastPrep-96 TM Procedure Cells and lysing buffer 1. Prepare lysis tube 2. Process with FastPrep-96 Instrument 3. Centrifuge to pellet debris 4. Extract & Purify 6

7 SuperFastPrep-2 Lyse wherever you are! Thorough grinding - Omnidirectional motion with a unique crankshaft-slider mechanism. Proceed up to 2 x 2 ml Lysing Matrix tubes. Time Saving - Complete sample lysis of most difficult samples in 5 to 15 seconds. Portable - Handheld system for lab and field use, with built-in battery power supply. No clean-up - Single-use Lysing Matrix tubes containing an O-ring seal. Complete solution - High number of ready-to-use Lysing Matrix tubes and wide range of purification kits for DNA, RNA and proteins designed to fit any sample and application. Dimension - Height: 116 mm; 330 mm Large; Width: 86 mm; Weight: 1kg. Cat. No. Description SuperFastPrep-2 Instrument Super Easy, Super Fast! Tube Support Plug Tubes Holder Figure 1: DNA from dried corn kernels homogenized in dry conditions with the SuperFastPrep-2 and Lysing Matrix A tubes containing garnet and two 1/4 inch ceramic beads. Figure 1 Analysis with a Nanodrop spectrophotometer and a Bioanalyser system from Agilent showed that highest quality and quantity of extracted DNA is obtained after 5 seconds grinding with the SuperFastPrep-2 instrument. 7

8 Adapters Many interchangeable Adapters for any Sample Size and Room Temperature for: FastPrep-24 5G, FastPrep-24 QuickPrep 24 x 2 ml samples (included with FastPrep-24 TM instruments) Cat # HiPrep 48 x 2 ml samples Cat # TallPrep 24 x 4.5 ml samples Cat # TeenPrep 12 x 15 ml samples Cat # High-Throughput for: FastPrep-96 2 x 96-deep well plate adapter (included with the FastPrep-96 Instrument) Cat # QuickFlex 96 x 2 ml samples Cat # TallFlex 48 x 4.5 ml samples Cat # Cryogenic Conditions for: FastPrep-24 5G, FastPrep-24 CoolPrep 24 x 2 ml samples Cat # CoolTeenPrep 6 x 15 ml samples Cat # CoolBigPrep 2 x 50 ml samples Cat # ConeFlex Use all FastPrep-24 5G Adapters with the FastPrep-96 Instrument 8

9 Extraction Temperature BigPrep 2 x 50 ml samples Cat # All Metal QuickPrep 24 x 2 ml samples Cat # All Metal TeenPrep 12 x 15 ml samples Cat # All Metal BigPrep 2 x 50 ml samples Cat # TeenFlex 20 x 15 ml samples Cat # BigFlex 8 x 50 ml samples Cat # LargeFlex 2 x 250 ml samples Cat # FastPrep System The Ultimate in Flexibility! 9

10 Lysing Matrix Experience unmatched versatility Thorough grinding of any sample on any bead beater! The FastPrep Lysing Matrices come in 16 unique matrix compositions to ensure leak and contamination free homogenization of any sample type without DNA/RNA shearing. FastPrep Lysing Matrices are critical components of the FastPrep sample preparation system. The size of the beads and their composition has been optimized for use with the FastPrep system to achieve the best results. Additionally, they have the advantage of being used on any high-speed bead-beating machines. Because size matters All Lysing Matrix tubes offered come in every size, from 2 ml - 50 ml depending on your sample size. Description Pack Size 2 ml 50, 100 and 500 x 2 ml tubes 4.5 ml 25, 50 and 100 x 4.5 ml tubes 15 ml 5, 25 and 50 x 15 ml tubes 50 ml 10, 50, 100 and 500 x 50 ml tubes 96-well plates (96 x 1.2 ml) 1 and 10 x 96-well plates Validated worldwide with 3,000+ Lysing Matrix specific publications - We develop solutions that deliver results! 10

11 Lysing Matrix Table Lysing Matrix Beads content Samples Extraction A B C D E G H I J K M S SS Y Z Garnet matrix and 1/4 inch (6.35 mm) ceramic spheres Animal, Bacteria, Yeast, Fungi, Plant 0.1 mm silica spheres Bacteria, Spores 1 mm silica spheres Yeast, Fungi 1.4 mm ceramic sphere Animal, Plant 1.4 mm ceramic spheres, 0.1 mm silica spheres, and one 4 mm glass bead 1.6 mm aluminum oxide particles and 1.6 mm silicon carbide particles 1.6 mm silicon carbide particles and 2 mm glass beads 2 mm glass beads and 2 mm yellow zirconium oxide beads 2 mm yellow zirconia beads and a 4 mm black ceramic sphere 2 mm yellow zirconia beads and 1.6 mm aluminum oxide particles Soil, Sediments, Water, Feces Plant, Animal, Bacteria, Molds, Fungi, Coral Emulsions, Fixed Samples Plant, Animal, Yeast, Fungi, Spores Plant, Animal, Wood, Seeds, Very Dense Soils and Clays, Bacterial, Aggregates, Whole Insects, Ticks, Ancient, and Dried Samples Plant, Animal, Wood, Seeds, Very Dense Soils and Clays, Whole Insects, Ticks, Ancient and Dried Samples, Dry Grinding Fungal Spores/Rusts Plant, Animal, Bacteria (Gram +/-), Molds, Fungi, Coral Emulsions, Fixed Samples. Fungal Spores and Rusts 0.8 mm zirconium silicate beads Tissues, Animal By products, Yeast Spores 1/4 inch (6.35 mm) ceramic sphere 1/8 inch (3.175 mm) stainless steel beads 1/4 inch (6.35 mm) stainless-steel grinding balls. 0.5 mm Yttria-stabilized Zirconium Oxide Spheres 2 mm Yttria-stabilized Zirconium Oxide Spheres Animal bone, teeth, skin, hair, seeds Tough Tissues: Skeletal/Muscle, Lung Heart, Bone, Seeds, Spores Bacteria, Yeast, Fungi, Soil, Feces, Nematodes Animal, Insect, Plant, Seeds DNA, DNA, RNA Virus isolation from tissue, Organelle, Mitochondria DNA, RNA DNA DNA Request your Lysing Matrix Box now on 11

12 Manual DNA, RNA & Protein Isolation Make your experiments easy with the FastPrep Extraction Kits Ready-to-Use Protocols for DNA, RNA and Protein Isolation from any Sample! Rapid and reproducible sample lysis and purification process No cross contamination with closed lysing matrix tubes Increased yields of high quality DNA, RNA and Integrity and size of DNA, RNA and are retained Nucleic acids and are ready-to-use in downstream applications DNA from Soil BEST SELLER FastDNA TM Spin Kit for Soil: 50x 2mL preps - Cat N , FastDNA TM 50 ml Spin Kit for Soil: 10x 50mL preps - Cat N Lysing matrix E (2mL) or garnet matrix (50mL) included No hazardous organic reagents required SPIN filters streamline silica handling FastDNA TM -96 Soil Microbe DNA Kit - Cat N x 96-well plates with Lysing matrix Y Up to 130mg of soil or sludge Yield: 5μg of total DNA eluted in μl DNA from Plant, Animal, Yeast, Fungi, Bacteria FastDNA TM Kit - Cat N FastDNA TM Spin Kit - Cat N x 2mL preps, includes Lysing Matrix A tubes No hazardous organic reagents required SPIN filters streamline silica handling (for FastDNA SPIN kit) FastDNA TM -96 Tissue & Insect DNA Kit (and blood) Cat N FastDNA TM -96 Fungal/Bacterial DNA Kit Cat N FastDNA TM -96 Plant/Seed DNA Kit Cat N x 96-well plates with Lysing matrix Y (Fungal/Bacterial DNA) or Z Up to 10mg tissue samples, 100μl whole blood, 20mg of wet weight fungal or bacterial, or 80mg plant per well DNA from Feces FastDNA TM Spin Kit for Feces - Cat N Includes Lysing matrix E, 50x 2mL preps Removes organic contaminates, such as humic acid, for downstream applications Achieves Optimal 260/280 ratios ( ) Typically isolates 10μg 20μg of DNA from 500mg of stool FastDNA TM -96 Fecal DNA Kit - Cat N x 96-well plates with Lysing matrix Y Up to 80mg wet or dry stool per well 12

13 from Bacteria FastProtein TM Blue Matrix - Cat N (50 preps) and (100 preps) 2mL Lysing Matrix B tubes Protein extracts are ready for immediate electrophoresis or purification Ideal for optimizing induction conditions GlycoProtein TM from Plant, Animal, Microbes FastGlycoProtein Isolation Kit ConA Resin - Cat N (10preps) FastGlycoProtein Isolation Kit WGA Resin- Cat N (10preps) 2mL Lysing Matrix A tubes Concanavalin-A lectin, for isolating most N-Linked glycans. Wheat Germ Agglutinin lectin for isolating O-Linked glycans that contain sialic acid or N-acetyl glucosamine. from Yeast and Fungi FastProtein TM Red Matrix - Cat N (50 preps) and (100 preps) 2mL Lysing Matrix C tubes and lysis buffer Protein extracts are ready for immediate electrophoresis or purification Ideal for optimizing induction conditions RNA from Plant and Animal FastRNA TM Pro Green Kit - Cat N x 2mL preps, includes Lysing Matrix D tubes Lyse up to 100mg tissue per 2ml tube RNA ready-to-use for downstream application (RT-PCR, Nothern Blot ) RNA from Bacteria FastRNA TM Pro Blue Kit - Cat N x 2mL preps, with Lysing Matrix B tubes For use with gram positive and gram negative bacteria Lyse up to cells per 2ml tube FastRNA TM Spin Kit for Microbes - Cat N No hazardous reagent 50x 2mL preps, with Lysing Matrix B tubes 2 consecutive purification steps on columns RNA from Yeast and Fungi FastRNA TM Pro Red Kit - Cat N x 2mL preps,lysing Matrix C tubes Lyse up to cells per 2mL tube RNA ready-to-use for downstream application (RT-PCR, Nothern Blot ) FastRNA TM Spin Kit for Yeast Cat N No hazardous reagent 50x 2mL preps, Lysing Matrix Y tubes included 2 consecutive purification steps on columns RNA from Soil FastRNA TM Pro Soil-Direct Kit - Cat N FastRNA TM Pro Soil-Indirect Kit - Cat N x 2mL preps, Lysing Matrix E tubes RNA extraction from soil ( direct ) or from soil supernatant ( indirect ) Humic acids reduced to allow RT-PCR Includes additional reagents for even further purification if necessary 13

14 Automated DNA/RNA extraction MPure-12 Process up to 12 samples in a true walk away system. Fully automated and integrated platform that offers cost and time savings Reproducibility, lot-to-lot consistency, scalability, ease-of-use & convenience Highest quality & yield of DNA & RNA for downstream applications Flexibility & simplicity: 1-12 samples from a wide range of bio-specimens processed in a single cycle. No cross-contamination of samples thanks to the unique design of the platform Minimized nucleic acid loss & degradation Increased safety & efficiency through a closed system The MPure-12 allows rapid purification of nucleic acids from a wide variety of any sample using magnetic bead separation technology. Combined with a uniquely designed magnetic bead processing chamber, the fully integrated and easy-to-use pre-packaged reagent kits offer superior yields of nucleic acids and high-quality results at an affordable price. Cat.No Description MPure-12 system MPurify your Nucleic Acid Request your FREE Demo Now! 14

15 High Quality, Reliable, Consistent Results! Cat. No. Product Name Sample Type Target DNA extraction for various cell types MPure-12 Blood DNA extraction kit 200 (small samples) MPure-12 Blood DNA extraction kit 1200 (large samples) MPure-12 Tissue DNA extraction kit MPure-12 Cultured Cell DNA extraction kit Human, animal blood (fresh, old, dried, frozen whole blood with common anticoagulants), buffy coat Human, animal blood (fresh, old, dried, frozen whole blood with common anticoagulants), buffy coat Human and animal tissue (fresh and frozen tissues), rodent tails, insects (fresh and frozen tissue), fish pins, shrimp legs Cell culture, plasma, serum, bone marrow, pure buffy coat (fresh or frozen serum/plasma, cells in adherent/suspension culture, lavage) gdna, mtdna, viral DNA gdna, mtdna, viral DNA gdna, mtdna gdna, mtdna MPure-12 FFPE DNA extraction kit FFPE (formalin-fixed, paraffin embedded) tissue sections gdna, mtdna MPure-12 Forensic DNA extraction kit Forensic material (whole blood, clotted blood, bones, teeth, ancient bones, hair roots, forensic surface and contact swabs, saliva, chewing-gum, cigarette butts, stamps, envelopes, tissues...) gdna, mtdna MPure-12 Plant DNA extraction kit Leaves and seeds (GMO), fungi gdna Viral DNA /RNA extraction MPure-12 Viral Nucleic Acid extraction kit (small samples) MPure-12 Viral Nucleic Acid extraction kit 800 (large samples) MPure-12 Viral RNA extraction Kit Viral /Pathogen DNA extraction MPure-12 Bacteria DNA extraction kit MPure-12 HPV DNA extraction kit for swab samples MPure-12 TB DNA extraction kit Total RNA extraction MPure Viral / Pathogen Nucleic Acids Extraction kit A MPure-12 Viral / Pathogen Nucleic Acids Extraction kit B MPure-12 Total RNA extraction kit Cell culture supernatant, human serum, plasma, urine, cerebrospinal fluid and other cell-free body fluids Cell culture supernatant, human serum, plasma, urine, cerebrospinal fluid and other cell-free body fluids Cell culture supernatant, human serum, plasma, urine, cerebrospinal fluid and other cell-free body fluids Bacteria species (from different kinds of starting material), bacteria pellets, paper points, liquid transport media, swabs and urine, colony Urogenital tract specimen collected by cervical brush or genital swab, cervicovagina lavage, urine Mycobacteria species (from different kinds of starting material), bacteria pellets, paper points, liquid transport media, swabs and urine, colony serum, plasma, cerebrospinal fluid, pretreated urine or other cell-free body fluid samples Urogenital tract specimen collected by cervical brush or genital swab, cervicovagina lavage, urine. Swabs and discharges collected from throat, rectum, buccal, nasal, pharyngeal, eye conjunctiva and erosive-ulcerative lesions of human skin and mucous membranes Human, animal blood (fresh, old, dried, frozen whole blood with common anticoagulants), plasma, serum, pure buffy coat, bone marrow, dried swab material (buccal, nasal, pharyngeal, vaginal, eye swab or saliva), cell culture (adherent or in suspension). Viral DNA / RNA Viral DNA / RNA Viral RNA Bacterial DNA Viral, Bacterial DNA Mycobacteria DNA Viral, Bacterial DNA/RNA Viral, Bacterial DNA / RNA Total RNA 15

16 MP Biomedicals, Your Partner in Life Science Research! France Tel: Fax: Poland Tel: Fax: Santa Ana, CA Worldwide Headquarters Strasbourg, France European Headquarters Parc d Innovation - BP Rue Geiler de Kaysersberg Illkirch Cedex custserv.eur@mpbio.com Tel: Fax : Germany Tel: Fax: The Netherlands Tel: Fax: United Kingdom Tel: Fax: Singapore Tel: Fax: Serbia Tel: Fax: Belgium Tel: Fax: Switzerland Tel: Fax: Moscow Tel: Fax: FastPrep TRADE IN Program TRADE, SAVE, OWN! Being either a FastPrep-24 or a FastPrep FP120 user or using any other bead beater available in the market, we are offering you the best condition to upgrade your homogenizer to the FastPrep-24 5G! Quick Easy Go Green! Contact us now to benefit from the BEST offer on F0216-2