Virtual Laboratory Bacterial Transformation

Transcription

1 Virtual Laboratory Bacterial Transformation Name: Before going to the Virtual lab, go to Bozeman site for AP Bio and watch: Lab 6 Molecular Biology *Note we have already done the Gel electrophoresis lab, so you only need to watch the first part of the video through the Transformation portion however, you MIGHT want to review the Gel electrophoresis portion for TBTQ purposes 1. What is the little bit of DNA that will be passed from one bacterium to another in this lab? 2. Draw and label the pglo structure that Mr. Anderson s lab uses. Indicate what each labeled part is for a. ori b. AMP c. GFP d. arac 3. What is the type of bacteria that will be used in the lab? 4. What is Luria broth for? 5. What does calcium chloride do to the bacteria in this lab? L. Carnes MVHS AP Biology Adapted from Pearson: LabBench Activities

2 6. SKETCH in the expected results of the experiment on the diagrams below. Explain what each result means for each plate. Don t forget to label which ones should be + DNA and which ones should be - DNA. NOW IT S TIME TO COMPETE THE VIRTUAL LAB In this laboratory you will use some basic tools of molecular biology to gain an understanding of some of the principles and techniques of genetic engineering.

3 Introduction 1. Log onto the Pearson LabBench web page at 2. Select Lab 6 Molecular Biology / Part I Transformation in Bacteria 3. Follow the instructions carefully on each screen be sure to review all animations. 4. Answer the lab packet questions as you proceed through the virtual lab. 1. Read Key Concepts I: Bacterial Transformation. What is bacterial transformation? a. What gene are we going to attempt to introduce into our bacterial strain? b. What will happen to our bacteria strain if we are successful? 2. Read Bacterial Colonies. What species of bacterial are we using for this experiment? 3. Read E. coli Bacteria. Where is E. coli bacteria commonly found? a. How does E. coli reproduce? How rapidly can this occur? b. Describe the chromosomal genome of E. coli. 4. Read Plasmids. What are plasmids? a. How are plasmids used in genetic engineering? b. What does the amp R gene confer resistance to in E. coli?

4 c. What happens to E. coli cells that do not carry the amp R gene? d. In order to transform cells...what do we need to do first? 5. Read Competent Cells. Describe what this concept means. a. What processes are used to make cells competent? b. In what phase of bacterial growth is it easiest to make cells competent? 6. Read Design of Experiment I. Make notes of some important safety procedures: 7. Read Transformation Procedure. After you've familiarized yourself with the procedure as a whole, take a closer look at each stage. Select steps 1 4 and 6 to see what is going on at the cellular level. THE VERY LAST A Closer Look screen shows an animation of the transformation procedure...watch IT! There are basically SIX steps to follow to correctly transform bacteria. Describe these steps IN ORDER below: a. b. c. d.

5 e. f. 8. The figures below show the events that take place during transformation of an E. coli cell. Type in the letters to indicate the correct order in which the events occur. 9. Read Analysis of Results I. Draw AND describe the four possible outcomes for this experiment in the space provided below. a. What does it mean if there is a lawn growth of cells? b. What does it mean if there are individual colonies of growth on the agar? c. What does it mean if nothing is growing on the agar?

6 10. Read Label the Results of Your Experiment. After incubation, the following plates were removed from the incubator. All but part of one label have been removed so that you must now use your understanding of this laboratory to make new labels for each plate. Based on the PHYSICAL APPEARANCE of each plate, select the appropriate label for the plates. DESCRIBE THIS BELOW...do NOT simply write A, B, C, or D! For example...plate I should be labeled because. a. Plate I: b. Plate II: c. Plate III: d. Plate IV: 11. Take Lab Quiz I. You may write your responses below: Question #1 Question #2 Question #3 Question #4 Question #5 Question #6 Analysis & Conclusions: Long Essay Describe: a. How recombinant DNA technology could be used to insert a gene of interest into a bacterium; b. Two ways in which recombinant bacteria could be identified; c. One way in which expression of the gene of interest could be ensured; d. Discuss how a specific genetically modified organism might provide a benefit for humans and at the same time pose a threat to a population or ecosystem.

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