Clarity and Clarity Max Western ECL Substrates

Transcription

1 Imaging and Max Western Substrates The Clear Choice in Chemiluminescent Western Blot Detection

2 and Max Western Substrates Get. The Clear Choice in Chemiluminescent Western Blot Detection

3 bio-rad.com/web/substrate bio-rad.com/web/max Ideal for digital and film-based imaging Excellent sensitivity Very low background levels Works with any HRP-compatible secondary antibody 1-year shelf life at room temperature Extremely long signal duration Allows optimization through multiple exposures Highest level of sensitivity Low femtogram level sensitivity Compatible with nitrocellulose, PVDF, and LF PVDF membranes Versatility Substrate replaces most other substrates. It can be confidently used with film and digital imagers, for detecting highand low-expressing proteins, and with short and long exposure times. Select Prime Plus Max Max Substrate provides higher sensitivity for low-expressing proteins. Femto Dura Digital Imager Film Forte Simplicity of Choice and Max meet all your western substrate needs. Bio-Rad Laboratories, Inc.

4 Western Substrate Substrate exhibits excellent visual limit of detection versus other commonly used substrates. Comparative studies were conducted with Thermo Fisher Scientific s Plus,, and, as well as GE Healthcare s. When factoring in background intensity levels, analysis of western blots reveals Western Substrate s superior signal-to-noise ratio and signal duration versus the market-leading chemiluminescent substrates (Figures 1 and 2). 00:00 hr A Plus Purified ADH, ng B Signal-to-noise ratio Plus Protein per well, ng Fig. 1. Comparison of chemiluminescent substrate sensitivity. A, five different western blots were loaded with the same protein loads of purified alcohol dehydrogenase (ADH). Blots were incubated with substrates per manufacturer recommendations, then imaged for 10 sec on the ChemiDoc MP Imaging System. B, the signal-to-noise ratio for each band is depicted in bar graph form. and Plus provided the best signal-to-noise ratios across all protein loads. 01:00 hr A Plus Purified ADH, ng B Signal-to-noise ratio Plus Protein per well, ng Fig. 2. Comparison of chemiluminescent substrate signal intensity. A, the same five blots were incubated with substrates per manufacturer recommendations, then after an hour were imaged for 30 sec on the ChemiDoc MP Imaging System; B, the signal-to-noise ratio for each band is depicted in bar graph form. and provided superior signal-to-noise ratios across all protein loads, whereas other competitor substrates exhibited a considerable loss in signal.

5 Max Western Substrate Max Substrate exhibits superior visual limit of detection versus other commonly used highperformance substrates on the market. Comparative studies were performed with GE Healthcare s Select and Prime and Thermo Fisher Scientific s SuperSignal West Femto and SuperSignal West Dura (Figure 3). Choose Max when you need more sensitivity than (Figure 4). A Max Purified IKB alpha, pg B Max Select SuperSignal West Femto Select Prime Signal-to-noise ratio SuperSignal West Femto 5 ND Protein per well, pg Fig. 3. Comparison of chemiluminescent substrate signal intensity. A, five different western blots were loaded with the same protein loads of purified IKB alpha. Blots were incubated with substrates per manufacturer recommendations, then imaged for 70 sec on the ChemiDoc Touch Imaging System. B, the signal-to-noise ratios for the bands in the boxed area for Max, Select, and SuperSignal West Femto are depicted in bar graph form. Max and Select provided the best signal-to-noise ratios across all protein loads. ND, not determined; signal at 30 pg for SuperSignal West Femto was not detectable above background noise. Purified IKB alpha, ng Max Fig. 4. Comparison of chemiluminescence for and Max Substrates. Blots were incubated with substrates as recommended and imaged for 70 sec on the ChemiDoc Touch Imaging System. Max Substrate provides additional sensitivity, allowing visualization of the lower protein concentrations. and Max Western Substrates

6 Ordering Information Catalog # Description Western Substrate, 200 ml, contains 100 ml Western Peroxide Reagent and 100 ml Western Luminol/ Enhancer Reagent Western Substrate, 500 ml, contains 250 ml Western Peroxide Reagent and 250 ml Western Luminol/ Enhancer Reagent Max Max Western Substrate, 100 ml, contains 50 ml Max Western Peroxide Reagent and 50 ml Max Western Luminol/Enhancer Reagent Protein Standards Precision Plus Protein All Blue Prestained Standards, 500 µl, 50 applications Precision Plus Protein Unstained Standards, 1 ml, 100 applications Precision Plus Protein WesternC Blotting Standards, 50 applications Buffers x Tris/Glycine/SDS, 1 L x Laemmli Sample Buffer, 10 ml Electrophoresis Cells Criterion Cell, includes electrophoresis buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, 26-well) Mini-PROTEAN Tetra Cell for Mini Precast Gels, 4-gel vertical electrophoresis system, includes electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam Power Supplies PowerPac Basic Power Supply, / V PowerPac Universal Power Supply, / V Catalog # Description Imaging Systems ChemiDoc MP Imaging System, gel imaging system, PC or Mac, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab Software ChemiDoc Touch Imaging System, gel imaging system, includes internal computer, 12 in. touch-screen display, camera, Image Lab Software, Chemi/UV/Stain-Free Sample Tray, Substrate, Precision Plus Protein Dual Color Standards ChemiDoc MP Red LED Module Kit, includes 2 epi-red LED modules, 1 red emission filter ChemiDoc MP Green LED Module Kit, includes 2 epi-green LED modules, 1 green emission filter ChemiDoc MP Blue LED Module Kit, includes 2 epi-blue LED modules, 1 blue emission filter Blotting System Trans-Blot Turbo Transfer Starter System, blotting instrument, includes base, 2 cassettes to hold 1 2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumables kit Trans-Blot Turbo Ready-to-Use Transfer Packs Trans-Blot Turbo Transfer Packs, mini, PVDF, pkg of Trans-Blot Turbo Transfer Packs, midi, PVDF, pkg of Trans-Blot Turbo Transfer Packs, mini, nitrocellulose, pkg of Trans-Blot Turbo Transfer Packs, midi, nitrocellulose, pkg of 10 Trans-Blot Turbo RTA (Ready-to-Assemble) Transfer Kits Trans-Blot Turbo RTA Transfer Kit, mini, nitrocellulose, for 40 blots Trans-Blot Turbo RTA Transfer Kit, midi, nitrocellulose, for 40 blots Trans-Blot Turbo RTA Transfer Kit, mini, PVDF, for 40 blots Trans-Blot Turbo RTA Transfer Kit, midi, PVDF, for 40 blots Trans-Blot Turbo RTA Transfer Kit, mini, LF PVDF, for 40 blots Trans-Blot Turbo RTA Transfer Kit, midi, LF PVDF, for 40 blots LF PVDF, low fluorescence polyvinylidene difluoride. 10-Well 10-Well 12-Well Description 30 μl 50 μl 20 μl Mini-PROTEAN TGX Stain-Free Precast Gels 10% % % Any kd All formats are available as both ten packs (catalog numbers listed) and two packs. To order as a two pack, add an S to the end of the catalog number for the corresponding ten pack Well* 18-Well 26-Well Description 45 µl 30 µl 15 µl Criterion TGX Stain-Free Precast Gels** 10% % % Any kd * Reference wells accommodate 15 µl of markers/standards. ** Criterion TGX Stain-Free Gels are sold as single gel packages. Additional gel formats are available. See bio-rad.com/web/criteriontgx for all gel formats. and Select are trademarks of GE Healthcare. Mac is a trademark of Apple Inc. Precision Plus Protein Standards are sold under license from Life Technologies Corporation, Carlsbad, CA, for use only by the buyer of the product. The buyer is not authorized to sell or resell this product or its components. SuperSignal is a trademark of Thermo Fisher Scientific Inc. Max Western Substrate is manufactured by Cyanagen Srl and is the subject of patent application numbers US , EP , US , AU , CA , US , and EP , together with other equivalent granted patents and patent applications in other countries like CN Bio-Rad Laboratories, Inc. Life Science Group Web site bio-rad.com USA Australia Austria Belgium 32 (0) Brazil Canada China Czech Republic Denmark Finland France Germany Hong Kong Hungary India Israel Italy Japan Korea Mexico The Netherlands 31 (0) New Zealand Norway Poland Portugal Russia Singapore South Africa 27 (0) Spain Sweden Switzerland Taiwan Thailand United Arab Emirates United Kingdom Bulletin 6305 Ver B US/EG Sig 1215