Applied Biosystems Thermal Cycler Remote Access User Guide

Transcription

1 Applied Biosystems Thermal Cycler Remote Access User Guide A step-by-step guide to setting up secure access to your thermal cycler with Thermo Fisher Connect

2 Check run status from any mobile device This guide will walk you through the process of setting up your Applied Biosystems SimpliAmp Thermal Cycler and ProFlex PCR System for remote accessibility through Thermo Fisher Connect. Check instrument availability anytime Table of contents Design and upload methods from anywhere Share methods with colleagues Ensure instrument is cloud-enabled 4 Create Thermo Fisher Connect account 5 Connect instrument to the Internet 5 Connect instrument to Thermo Fisher Cloud 6 Set up mobile device to connect to instrument 7 Check run status from mobile device 8 Design methods from desktop computer 10 Applied Biosystems thermal cyclers 13 PCR enzymes 14 PCR plastics 15

3 Ensure instrument is cloud-enabled Any SimpliAmp Thermal Cycler or ProFlex PCR System can be connected to the Thermo Fisher Cloud as long as it is using firmware version 1.2 or higher. You can check your current firmware version by: 1. Going to the Settings menu. 2. Selecting About Instrument. If the instrument does not have firmware version 1.2 or higher, you will need to upgrade your firmware, using the USB provided or by going to thermofisher.com/simpliamp or thermofisher.com/proflex 1. Insert USB preloaded with new firmware into the USB port. 2. Go to the Settings menu, or touch the shortcut to the Upgrade Firmware screen if you have enabled the USB pop-up. 3. Go to Maintenance & Services, then Software Update. 4. Select the appropriate file and touch Update and Yes to confirm that you want to upgrade. IMPORTANT! Do not power off the instrument during the upgrade. When the upgrade process is complete, the instrument will restart automatically. Create Thermo Fisher Connect account 1. From your Internet browser, go to thermofisher.com/connect 2. If you already have an account with Thermo Fisher Scientific, log in using the same name and password. 3. If you do not already have an account, follow the prompts to create one. 4. Once you have logged in to thermofisher.com/connect, an will automatically be sent to you to verify your address. 5. Open your and click on the link to visit the Connect page to verify your account. 6. If you have done this successfully, you will receive a confirmation telling you that you have connected to Thermo Fisher Connect. Connect instrument to the internet SimpliAmp and ProFlex Thermal Cyclers can be connected to the Internet using a wireless (using the High-Power USB Wi-Fi Module, Cat. No. A26774) or wired connection. See the product manual for detailed instructions on setting up either connection type. 4 5

4 Connect instrument to Thermo Fisher Cloud 1. Touch Sign In. 2. Select Administrator when asked to choose screen name. 3. Log in. (The default password for the Administrator account is password.) 4. You will then be redirected back to the home screen. Go to Settings. 5. Select Remote Service. 6. Turn on Remote Service. 7. Touch My Profile on the home screen. 8. Touch the Cloud tab and enter your Thermo Fisher Connect account login and password. 9. Select Link Account. Upon successful authentication, the Administrator is linked to Thermo Fisher Connect. Set up mobile device to connect to instrument Download the Instrument Connect Mobile Application on your mobile device. 1. For an ipad or iphone mobile device, download the application from the App Store by searching for Instrument Connect. 2. For Android devices, download the application from Google Play by searching for Instrument Connect. 3. Log in to the Instrument Connect mobile application using your Thermo Fisher Connect login and password. Subsequently, any user can log in and link the instrument to their Thermo Fisher Connect account by following steps 3 9 above. NOTE: The administrator account must be activated first to connect the instrument to the Cloud. Afterward, multiple users can create unique user profiles using their own login information. Each unique profile must have a unique Thermo Fisher Connect account. 6 7

5 Check run status from mobile device 1. Open the Instrument Connect app. If login is required, enter your Thermo Fisher Connect login and password. 2. The dashboard is displayed upon login, showing the status of all registered instruments. 3. To check the time remaining and the protocol stage for a running instrument, click on it. 8 9

6 Design methods from desktop computer and upload to instrument 1. From your Internet browser, go to thermofisher.com/connect 2. Log in using your Thermo Fisher Connect login and password 3. Select the PCR Designer application from the dashboard. 5. Follow these steps to create a new experiment (method): a. Enter experiment properties. Click Next. b. Enter the stages and steps in the method. Click Next. 4. Select Create new experiment. (At a later time Open existing experiment may be selected to view templated protocols and those you ve already written.) 10 11

7 c. Define plate layout details. Click Next. Applied Biosystems thermal cyclers Since we introduced the first thermal cycler in 1987, our engineers have continued to design, develop, and support innovative PCR instruments to empower your research. Applied Biosystems thermal cyclers are renowned for their reliability, accuracy, and user-friendly interfaces. d. Save the method when prompted. 6. Download the new method on your thermal cycler(s) at your convenience. a. Log in to the thermal cycler. b. View the list of protocols saved to your Thermo Fisher Connect account. ProFlex PCR System Flexible, high throughput perform up to three independent runs at once Veriti Thermal Cycler For robust thermal cycling and diagnostic applications SimpliAmp Thermal Cycler Elegant design, smart choice easy-to-use, compact, and accurate c. Select the desired method. (It will be downloaded to the instrument.) d. Run method. e. If you wish, check the status of the run from your mobile device Thermal Cycler Economical option for everyday cycling Automated Thermal Cycler Designed for easy robotic integration 12 Learn more at thermofisher.com/thermalcyclers 13

8 PCR enzymes We offer a comprehensive portfolio of Invitrogen PCR enzymes and master mixes with the high performance you need. Start with the selection guide below to find the best enzyme for common PCR applications. PCR plastics Applied Biosystems PCR plastics are designed and built for precision and reliability with our thermal cyclers. Visit our selection guide at thermofisher.com/appliedbiosystemsplasticsselectionguide PCR type Recommended DNA polymerase Fidelity vs. Taq polymerase High-fidelity PCR Invitrogen Platinum SuperFi DNA Polymerase Hot-start PCR Invitrogen Platinum Taq DNA Polymerase >100x 1x 1x Hot start Yes Yes No Standard PCR Invitrogen Taq DNA Polymerase Target length Up to 20 kb* Up to 5 kb Up to 5 kb Formats available Stand-alone enzyme Master mix Colorless, green** Colorless, green* Colorless, green* Colorless, green* Colorless Colorless * Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design. ** Direct gel loading with green buffer options. Find more options at thermofisher.com/pcrenzymes 14 15

9 Find out more at thermofisher.com/thermalcyclers For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. App Store, iphone, and ipad are trademarks of Apple Inc. Android and Google Play are trademarks of Google, Inc. COL