CYTAG CGH Labeling Kits

Transcription

1 CYTAG CGH Labeling Kits Optimized Labeling for Microarrays enzolifesciences.com

2 PIONEERS IN LABELING & DETECTION Delivering Superior Results for Better Understanding of Genetic Disorders, Cancers and Other Diseases Caused By Genomic DNA Copy Number Variations is a unique company with assay development know-how, backed by intellectual property, production, distribution, validation, and service capabilities. All of these skills combined in one company allow for the presentation to the market of open assay solutions that enable scientists to accurately and efficiently perform their cutting-edge research. Our extensive intellectual property portfolio allows us the freedom to operate, and our technical expertise enables us to provide optimized solutions to a multitude of platforms. Included in our extensive IP portfolio are nucleic acid labeling chemistries that support s position as a recognized pioneer in labeling and detection in genomics. A pillar of our molecular biology portfolio is our array-based comparative genomic hybridization (array CGH) labeling kit. Array CGH is a powerful tool for detecting gene copy number gains and losses associated with chromosome abnormalities. Detecting chromosomal aberrations by array CGH is faster, more robust, and provides superior results over other technologies such as FISH and G-banding karyotyping, thus providing a greater understanding of the role of chromosomal changes in genetic diseases and cancers. Complete Solutions for CGH Analysis and CGH+SNP Analysis CYTAG CGH Labeling kits for labeling DNA for CGH microarray analysis. Available in 20 and 200 reactions format. CYTAG TotalCGH Labeling kits for CGH or CGH+SNP microarray analysis. Available in 20 and 200 reactions format. KIT COMPONENTS ENZ K010 (2 x 10) CYTAG CGH Labeling Kit ENZ K100 (2 x 100) ENZ (2 x 10) CYTAG TotalCGH Labeling Kit Primers/Reaction Buffer 1 x 400 µl 4 ml 1 x 400 µl 4 ml Cyanine 3-dUTP Nucleotide Mix 2 x 50 µl 1 ml 2 x 50 µl 1 ml Cyanine 5-dUTP Nucleotide Mix 2 x 50 µl 1 ml 2 x 50 µl 1 ml Klenow DNA Polymerase 1 x 20 µl 0.2 ml 1 x 20 µl 0.2 ml Stop Buffer 1 x 100 µl 1 ml 1 x 100 µl 1 ml Nuclease-free Water 1 x 1 ml 10 ml 1 x 1 ml 10 ml ENZ (2 x 100) Alul 1000 units 1000 units RsaI 1000 units 1000 units PCR & Gel Clean-up Columns 20 tests 200 tests

3 ARRAY CGH WORKFLOW DNA Sample Reference gdna and Test Sample DNA Genomic DNA (human), male (ENZ-GEN106) Genomic DNA (human), female (ENZ-GEN107) Sample Preparation CGH No Preparation CGH+SNP Restriction enzyme digestion using Alul (ENZ-GEN108) Rsal (ENZ-GEN109) Sample Labeling LABELING LABELING Labeling of the DNA samples Purification of labeled DNA using ENZ-GEN100 Test DNA vs. Reference DNA Random prime label two DNA samples with the system; One with Cyanine-3 the other with Cyanine-5 Microarray Processing Preparation for hybridization HYBRIDIZATION HYBRIDIZATION Hybridization Reference DNA Combine equal amounts Hybridize Combine probe to equal microarray Oligo array scanning and analysis amounts Hybridize probe to microarray Block labeled repetitive sequences with Cot-1 DNA Test DNA Identify genomic aberrations Identify genomic aberrations Superior labeling delivers clear and accurate data analysis Superior Results Analysis of syndromic DNA using an oligonucleotide microarray (Agilent 4x180K) demonstrated the characteristic deletion in 15q11.2-q13 (chromosome 15) found in patients with Prader- Willi syndrome.

4 PROPRIETARY LABELING TECHNOLOGY CYTAG CGH Labeling Kits (ENZ-42671) Superior labeling assures confident aberrant calls and reduces the number of failed runs Lowest DLR scores ( ) assure confident aberrant calls and reduces the number of failed runs Superior dye incorporation and high specific activity deliver better hybridization results Suitable for challenging samples including formalin-fixed paraffin embedded (FFPE) tissue Extensively tested on all commonly used array scanner platforms (including Agilent, Nimblegen, and Innopsys) Validated by customers on common microarrays (including Agilent and OGT) Wide range of input DNA ( µg) s proprietary labeling technology with superior dye incorporation leads to the highest specific activity. Widely Trusted. Highly Cited. pmol dye incorporated per rxn Cy3 Cy5 Dye Dye Incorporation Cy3 Cy5 Cy3 Cy5 Competitor A Kit 1 Competitor A Kit 2 Figure 1: Four replicate 500 ng DNA samples were labeled with s CYTAG CGH Labeling Kit for Oligo Arrays or a leading competitor s kits. s proprietary labeling technology generates the highest specific activity of labeling. Adenoid Cystic Carcinoma Costa, AF et. al. Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression. A.F. Lab. Invest. (2014) 94, 692. Breast Cancer Schouten, PC et. al. Breast cancers with a BRCA1-like DNA copy number recur less often than expected after high-dose alkylating chemotherapy. Clin. Cancer Res. (2015) 4, 763. Discovery of Tumor Suppressor Genes and Oncogenes Protopopov, A et. al. Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic. Methods in Molecular Biology, (2008) 439, 87. Metastatic Melanoma Moore, S et. al. Detection of Copy Number Alterations in Metastatic Melanoma by a DNA Fluorescence in situ Hybridization Probe Panel and Array Comparative Genomic Hybridization: A Southwest Oncology Group Study (S9431). Clin Cancer Res 2927 (2008) 14, Postnatal Testing for Genome Imbalance Ahn et. al. Validation and implementation of array comparative genomic hybridization as a first line test in place of postnatal karyotyping for genome imbalance. Molecular Cytogenetics (2010), 3:9. Prenatal Testing for Chromosome Imbalance Ahn, JW et. al. A new direction for prenatal chromosome microarray testing: software-targeting for detection of clinically significant chromosome imbalance without equivocal findings. Peer J. (2014) 2, e354. A.L. Mosca-Boidron, et. al. (2013) An improved method to extract DNA from 1mL of uncultured amniotic fluid from patients at less than 16 weeks gestation. PLoS One 8, e Salivary Gland Carcinosarcoma Vékony, H et. al. Salivary gland carcinosarcoma: Oligonucleotide array CGH reveals similar genomic profiles in epithelial and mesenchymal components. Oral Oncology (2009) 45, 259. Solitary Median Maxillary Central Incisor Syndrome Szakszon, K et. al. Endocrine and anatomical findings in a case of Solitary Median Maxillary Central Incisor Syndrome. (2012) 55, 109. Uveal Melanoma Worley, LA et. al. Transcriptomic versus Chromosomal Prognostic Markers and Clinical Outcome in Uveal Melanoma. Clin Cancer Res. (2007) 13, pmol of dye incorp. / µg of DNA Cy3 Cy5 Specific Specific Activity Cy3 Cy5 Cy3 Cy5 Competitor A Kit 1 Competitor A Kit 2 Optimized & Trusted Kit for Prenatal Samples Lowest DLR Scores Results in Fewer Failed Runs Learn how the CYTAG CGH Labeling Kit can be used to label small volume samples (300 ng in 20 μl labeling volume). Application Note: CGH Labeling Protocol for Prenatal Small Volumes of DNA Cy5 Cy3

5 TOTAL SOLUTION FOR CGH+SNP ARRAYS CYTAG TotalCGH Labeling Kits (ENZ-42674) Superior labeling efficiency and better dye incorporation results in more confident calls for CGH+SNP arrays All-inclusive kit containing optimized CGH labeling reagents and ancillary products for CGH+SNP arrays. Kit includes: CYTAG CGH Labeling Kit Restriction enzymes Alul and Rsal PCR & Gel Clean-up Columns s Superior Labeling Results in Less Background Competitor A Comparative analysis of chromosome 15. Upon scanning, the quality of the labeling in DNA samples was visually inspected to demonstrate detection of known deletion in the Prader-Willi DNA. This demonstrates the superior labeling obtained with s CYTAG TotalCGH Labeling Kit when compared with Competitor A. s Kit Clearly Identifies SNPs Competitor A SNP analysis of a segment of chromosome 2 of DNA labeled with the s CYTAG TotalCGH Labeling Kit or with Competitor A s kit. s kit cleanly identifies the sequence at single nucleotide polymorphisms (SNPs). Quantification of the SNP analysis of DNA labeled with Competitor A s labeling system are not as clean.

6 RELATED PRODUCTS PRODUCT # SIZE BIOSCORE Screening & Amplification Kit ENZ reactions PCR & Gel Clean-up Kit ENZ-GEN , -0050, , 50, 200 tests Genomic DNA (human), male ENZ-GEN µg Genomic DNA (human), female ENZ-GEN µg Human Cot DNA ENZ-GEN µg Alul ENZ-GEN units Rsal ENZ-GEN units Global Headquarters ENZO LIFE SCIENCES, INC. 10 Executive Blvd. Farmingdale, NY Ph: Fax: info-usa@enzolifesciences.com European Sales Office ENZO LIFE SCIENCES (ELS) AG Industriestrasse 17 CH-4415 Lausen, Switzerland Ph: Fax: info-eu@enzolifesciences.com LOCAL EUROPEAN OFFICES Belgium, The Netherlands & Luxembourg Life Sciences BVBA Avenue Louise 65/Box Bruxelles Belgium Ph: Fax: info-be@enzolifesciences.com France Life Sciences (ELS) AG Branch Office Lyon 13, avenue Albert Einstein, F Villeurbanne, France Ph: Fax: info-fr@enzolifesciences.com Germany Life Sciences GmbH Basler Strasse 57a DE Lörrach Germany Ph: Fax: info-de@enzolifesciences.com UK & Ireland Life Sciences (UK) Ltd. 1 Colleton Crescent Exeter EX2 4DG Ph: (UK customers) Ph: Fax: info-uk@enzolifesciences.com For local distributors and detailed product information visit us online: Put our experience to work for you! Our broad range of scientific expertise and manufacturing capabilities enables us to provide innovative tools for CGH analysis to save you time and money! MB