BactiBlock Competitors Overview

Transcription

1 Doc by: NBM R&D Department Date: September 22

2 TABLE OF CONTENTS. Introduction 2. Summary 3. Complete Report

3 . Introduction During the last 3 years, NBM has evaluated several samples from different competitors within the silver based antimicrobial market. 4 main technologies are present in this market: Technology Country Brand Silver Zeolites USA Japan Japan Brand A Brand B Brand C Silver Glass Japan Silver Salts Japan USA Brand E Brand F Silver-Bentonite (Clays) Spain BactiBlock

4 2. Summary The full comparison test carried out against a sample in 22 can be used as an example of BactiBlock main competitive advantages, which is based on the use of phyllosilicates, NBM s proprietary technology: Excellent dispersion and processability Fast acting Minimum rheological influence High antimicrobial effectiveness These attributes place NBM s BactiBlock in an adventagous position for entering in different market sectors where real/life fast acting antimicrobial efficiency is needed. Also, the excellent processability and minimum rheological influence of the additive, means that BactiBlock has been proved to be an important advance for the Masterbatch / polymer industry and it is truly appreciated by converters and processing companies working with the material. Nanobiomatters dedicates a substantial effort to continuous improvement of the BactiBlock products and is currently in the process of launching new product grades with further improved characteristics for the different segments of this growth market. Technology type Silver based Zeolites Silver based glass Silver based glass Silver based BENTONITES Characteristics / Brands A B C D E F G Particle Shape Cubic Structures (3D) Amorphous Spherical (3D) Amorphous Crystals (3D) Plateletes (2D) Particle size (product range) , Being measured NA 5-6 Dispersability Medium to difficult Medium Difficult Very easy Silver control release Medium Medium Medium Low Medium Medium High Content of silver (product range),3-2,5%,3-,%,3-,%,5-2,%,-2,%,-,5%,5-3,%(**) Minimun Inhibitory Concentration (MIC, product range) ND 25 published.-. analyzed 25-5 published. >. ppm ND 3-5 ppm Effectiveness (short term) <24 hours Very low Very low Very low Low Low Medium Very high Effectiveness (long term) >3 months Medium Medium Medium High Medium Low High Remarks (EHS, handling, others) EPA Approved SIAA SIAA EPA Approved SIAA EPA Approved EPA Approved / SIAA (*) Standard Grade. New products in progress with Particle Size < μm (**) Standard application is % loading Main Competitive Advantages: Excellent dispersion & Procesability Fast acting Minimal rheological influence MIC Values obtained according to the SIAA Method High effectiveness 2

5 BactiBlock Showed the lowest Minimum Inhibitory Concentration (MIC) values - Good quality with % effectiveness Effectiveness in Microorganisms: Staphylococcus aureus and Escherichia coli Significant antimicrobial properties according to the results obtained under JIS Z 28 evaluation. Competitor Brand E (silver salts) Showed high MIC values. - Weak antimicrobial ability and lower effectiveness No significant reduction (antimicrobial properties) compared to the BactiBlock and control samples. No significant reduction (antimicrobial properties) compared to the BactiBlock and control samples. Excellent performance and compatibility with many polymers. BactiBlock dispersion in PP and PA polymers showed excellent condition compared to the Brand E sample. Good performance and good antimicrobial results in PA, but not effective in Polypropylene. Excellent antimicrobial and compatibility results with.5% silver concentration due to silver-clay synergies and dispersability. Higher silver content, Ag =.8% Test carried out in NanoBioMatters test laboratories, April 22 3

6 3. Complete report and extracts from full analysis. Excellent Antimicrobial effectiveness of BactiBlock according to Minimal Inhibitory Concentration (MIC) Minimal inhibitory concentration (MIC) is the lowest concentration of an antimicrobial agent that inhibits visible growth, after incubation period (usually 8 to 24 h). According to SIAA standards and the adapted macro dilution method, an antimicrobial agent MIC must be under 8 ppm. Minimum inhibitory concentrations are important in diagnostic laboratories: To confirm resistance of microorganisms to the antimicrobial agent. To determine the antimicrobial quality: a lower MIC is an indication of a better antimicrobial agent. BactiBlock and Competitor Brand E comparison MIC analysis according to SIAA Methodology Microorganism R % reduction Product Concentation MIC Comments Escherichia coli (CECT 56, ATCC 8739),4 96,2%,g/mL TSB (. ppm) MIC value thousand times higher than BactiBlock Antimicrobial efficacy not enough for Escherichia coli growth inhibition. Staphilococcus aureus (CECT 86, ATCC 26) 2,95 99,89%,g/mL TSB (. ppm) MIC value thousand times higher than BactiBlock Against Staphylococcus aureus the product showed slower growth inhibition than BactiBlock Escherichia coli (CECT 56, ATCC 8739) -,25 78,57%,g/mL TSB (. ppm) Test is repeated with lower concentration. To ppm, showed no antimicrobial effect Staphilococcus aureus (CECT 86, ATCC 26),2 24,57%,g/mL TSB (. ppm) Test is repeated with lower concentration but showed no antimicrobial effect Incubation: 37ºC, 24h 4

7 MIC VALUES Product Reference BB R.43 BB R.47 BB S.9 Brand E Broth TSB TSB TSB TSB Active ingredient Ag (,5%) Ag (,5%) Ag (3%) Ag (,8%) S.aureus 3 < E.coli >. MRSA Not checked P.aeruginosa Not checked MIC VALUES Product Reference BB R.43 BB R.47 BB S.9 Brand C Brand B Broth PW PW PW PW? Active ingredient Ag (,5%) Ag (,5%) Ag (3%) S.aureus <3 <3 < E.coli 3 <3 < ,5 MRSA <3 <3 <3 5 P.aeruginosa 3 3 < ,5 Test conditions: Nutrient medium: Peptone Water and TSB Bacterial initial concentration: 5x 4 -x 5 cells/ml BactiBlock concentrations (ppm):, 3, 5,, 3, 5, 8. Adjustment of the ph was not necessary since MHB+Ba 5

8 According to the standard method described by the Clinical and Laboratory Standards Institute (CLSI), minimal inhibitory concentration (MIC) is the lowest concentration of an antimicrobial agent that inhibits visible growth, after a predetermine incubation period (usually 8 to 24 h) 6

9 . Evaluation in thermoplastics: Antimicrobial properties of PP and PA with BactiBlock and Competitor Brand E were evaluated according to JIS z 28 standard: Note: On the left: PP + 2% Brand E; On the right: PA + % Brand E Sample Description Microorganism R Comments PP Sabic + 2% Brand E Escherichia coli (CECT 56, ATCC 8739) -,4 Shows no antimicrobial properties in PP PP Sabic + 2% Brand E Staphylococcus aureus (CECT 86, ATCC 26) -,3 Shows no antimicrobial properties in PP PA6 + % Brand E Escherichia coli (CECT 56, ATCC 8739) 6,26 Good performance in PA6 PA6 + % Brand E Staphylococcus aureus (CECT 86, ATCC 26) 3, Good performance in PA6 7

10 Comparison with BactiBlock S.9 inorganic grade: Sample Description PP Sabic + % BactiBlock S.9 PP Sabic + % BactiBlock S.9 PA 6+ % BactiBlock S.9 PA 6+ % BactiBlock S.9 Microorganism Escherichia coli (CECT 56, ATCC 8739) Staphylococcus aureus (CECT 86, ATCC 26) Escherichia coli (CECT 56, ATCC 8739) Staphylococcus aureus (CECT 86, ATCC 26) R 4,2 2,37 5,76 3,46 Conclusion: all BactiBlock samples comply with the requirements of the JIS z 28 (ISO 2296) international Standard for antimicrobial efficacy testing to a level of just %, which could be reduced in commercial applications in different polymer materials. 8

11 Summary Table: Escherichia coli (CECT 56, ATCC 8739) Comparison Chart Dosage Resin Specification CFU/mL R Ag (ppm) Control 2,% PP mm plate BactiBlock S.9,% PP mm plate 4,2 3 Brand E 2,% PP mm plate,97e+7,4 36 Staphylococcus aureus (CECT 86, ATCC 26) Comparison Chart Dosage Resin Specification CFU/mL R Ag (ppm) Control 2,% PP mm plate BactiBlock S.9,% PP mm plate 2,37 3 Brand E 2,% PP mm plate 9,39E+5,4 36 Escherichia coli (CECT 56, ATCC 8739) Comparison Chart Dosage Resin Specification CFU/mL R Ag (ppm) Control 2,% PA6 mm plate BactiBlock S.9,% PA6 mm plate 5,76 3 Brand E 2,% PA6 mm plate,e+ 6,26 36 Staphylococcus aureus (CECT 86, ATCC 26) Comparison Chart Dosage Resin Specification CFU/mL R Ag (ppm) Control 2,% PA6 mm plate BactiBlock S.9,% PA6 mm plate 3,46 3 Brand E 2,% PA6 mm plate 8,73E+2 3, 36 Conclusions: BactiBlock samples in the 2 polymers showed excellent dispersion compared to the Brand E sample. The incorporation of BactiBlock in the studied polymers showed significant antimicrobial properties. According to the results obtained with PP samples (under JIS 28 evaluation), the Brand E presented no significant reduction in bacterial growth compared to the control sample. The samples with BactiBlock showed color changes: brownish in PP and yellowish in PA6, while Brand E sample turned into light brownish in PP and light yellowish in PA6. NBM R&D Department has developed a second range of products, where the colour properties of the materials improved significantly. 9

12 4. 4. BactiBlock VS Silver Glass Techonology () Competitor D is a silver glass. The selected grade contains.6% of Ag from the complex silver-magnesiumaluminum phosphate. MIC values according to the Macrodilution method (nutrient medium: TSB, temp: 37ºC, incubation time: 24 h, static conditions): Product Microorganism CFU/mL CFU/mL mean UFC/mL Product (ppm) Silver (ppm) S. aureus 4,24E+7 4,24E+7 4,24E S. aureus 2,88E+7 2,88E+7 2,88E+7. 6 S. aureus 2,56E+7 3,2E+7 2,88E+7. 6 CONTROL S. aureus 6,8E+7 6,8E+7 E. coli 2,E+8 2,2E+8 2,E E. coli 2,E+8 2,2E+8 2,5E+8. 6 E. coli,92e+7 2,4E+7 2,6E+7. 6 CONTROL E. coli,4e+8,4e+8 BB R.43 S. aureus,37e+4,42e+4,4e+4 3,48 BB R.43 E. coli,e+ 5,8 BB R.47 S. aureus,26e+4,26e+2 6,36E+3 3,48 BB R.47 E. coli,e+ 5,8 BB S.9 S. aureus 3,6E+3 6,5E+3 5,5E+3 7,2 BB S.9 E. coli 3,8E+3 3,5E+4 3,65E+4 3 4,8 Antimicrobial effectiveness of HDPE films with antimicrobial additives against the growth of Staphylococcus aureus (CECT 86, ATCC 26) according to JIS Z 28 (ISO 2296) (see Annex I): Matrix Additive Sample % Additive AS* (ppm) R HDPE Alcudia TR 35 BactiBlock R.43 Film 8 3, 5 >2, HDPE Alcudia TR 35 BactiBlock S.9 Film, 32 >2, HDPE Alcudia TR 35 Sheet 2,8 48,3 HDPE Alcudia TR 35 Sheet 2,6 296,32 HDPE Alcudia TR 35 Sheet 2 3, 555 2,5 *AS: active sustance (Ag, silver)

13 The sample with silver glass technology is considered to present antimicrobial properties according to the Standard only when a concentration of 3% of additive is incorporated into the polymer. Therefore, a higher concentration of silver is required in samples with (555 ppm) compared to the sample with Bactiblock S.9 (32 ppm). E.coli growth curves according to JIS Z 28 trial log CFU/mL time (h) PP+,2% (Control) PP+% BactiBlock S.9,2% PP+,5%,2% Chimasorb

14 5. BactiBlock VS Silver Salt Techonology (Brand F) A masterbach (PP + % of active ingredient) was received by NanoBiomatters. The mineral charge is zirconium phosphate. The content of silver in the masterbach is.23%. The sample was diluted in PP (Sabic PHC 3) and, subsequently, films with μm of thickness were obtained. The antimicrobial effectiveness was evaluated against the growth of Staphylococcus aureus (CECT 86, ATCC 26) according to JIS Z 28 (ISO 2296) (see Annex I): Additive % Additive % PP Sabic PHC 3 Ag (ppm) R Brand F Masterbach ,49 Brand F Masterbach 2,5 97,5 6 Brand F Masterbach BactiBlock R.43 2,2 97,8 3,65 BactiBlock S ,49 BactiBlock S.9,75 99, The samples with Brand F and Bactiblock S.9 show antimicrobial properties with similar contents of silver. Furthermore, the sample with Bactiblock R.43 presents a high antimicrobial effectiveness with lower contents of silver due to a better stabilization of the active ingredient in this particular formulation. Therefore, long term effectiveness (>3 months) is better in the reference Bactiblock R.43. 2

15 5. BactiBlock VS Silver Water-based Techonology (Brand G) Brand G contains.24% of Ag. It is a silver water-based product and other elements such as Si, Fe, Cl were also detected by chemical analysis. The antimicrobial effectiveness of different concentrations of the product were analyzed against the growth Staphylococcus aureus (CECT 86, ATCC 26) according to the Macrodilution method (nutrient medium: TSB, temp: 37ºC, incubation time: 24 h, static conditions): Sample UFC/mL UFC/mL Mean R % reduction ppm Ag Method conditions Control 9,6E+7,E+8 9,8E Brand G 3,8E+8 3,76E+7 2,9E+8 -,33-3,6 24,g/mL TSB* Brand G 6,58E+7 6,22E+7 6,4E+7,9 34,69 2,4,g/mL TSB Brand G 4,4E+7 3,72E+7 3,88E+7,4 6,4,36,3g/2mL TSB BactiBlock R.43,E+ 7,99, 5,g/mL TSB BactiBlock R.43,E+ 7,99, 25,5g/mL TSB BactiBlock R.43,E+ 7,99, 5,g/mL TSB BactiBlock R.43,24E+5,28E+5,26E+5 2,89 99,87,75,3g/2mL TSB BactiBlock S.9,E+ 7,99, 3,g/mL TSB BactiBlock S.9,E+ 7,99, 3,g/mL TSB BactiBlock S.9,2E+8,6E+7 6,8E+7,6 99,87,45,3g/2mL TSB For a similar concentration of silver, a high antimicrobial effect is observed in samples with Bactiblock. Nevertheless, none of the competitor Brand G samples led to a significant antimicrobial effect. Probably in Brand G samples, which consist in a silver dissolution/dispersion, the silver is less stabilized and, therefore, less active. 3

16 7. BactiBlock VS silver and zinc based technology Masterbach (Brand H) Sample Ag (%) Zn (%) MB Brand H- MB Brand H-2 MB Brand H-3,7 2,42 78,2 The antimicrobial effectiveness of.3 g of product in 2 ml of TSB (5 ppm) were evaluated against the growth of Staphylococcus aureus (CECT 86, ATCC 26) according to the Macrodilution method (nutrient medium: TSB, temp: 37ºC, incubation time: 24 h, static conditions): Sample UFC/mL UFC/mL Mean % reduction Ag (ppm) MB-,24E+8,4E+8,33E+8 63,72,25 MB-2,42E+8,33E+8,37E+8 62,5,3 MB-3,87E+8,6E+8,73E+8 52,56,6 BactiBlock R.43 6,6E+4,E+ 3,3E+4 99,99,75 Control 3,36E+8 3,66E+4 The Bactiblock samples, despite their lower amount of silver, showed better antimicrobial properties than MasterBach Brand H samples. 4

17 8. BactiBlock VS Silver Zeolites Technology (Brand B) Zeolites with 62% of Zn and,3% of Ag Microorganism CFU/mL CFU/mL Mean R % red Product concentration Escherichia coli (CECT 56, ATCC 8739),E+,E+,E+ 8,43,,g/mL TSB (. ppm) Escherichia coli (CECT 56, ATCC 8739) Staphilococcus aureus (CECT 86, ATCC 26) Staphilococcus aureus (CECT 86, ATCC 26) 3,5E+8 3,5E+8 3,5E+8 -,2-3,6 8,8E+4 5,28E+5 3,8E+5 3,7 99,9 3,5E+8 3,5E+8 3,5E+8, 2,78,g/mL TSB (. ppm),g/ml TSB (. ppm),g/ml TSB (. ppm) For both microorganims MIC values for this particular products. and. ppm. As reported before, MIC values in TSB are lower for Bactiblock products (3-7 ppm). 5

18 8. BactiBlock VS Silver Zeolites Technology (Brand A) Antimicrobial tests The panels with higher levels of competitor s Brand A samples, BB R.43 and Bactiblock S.9 were sent to an external lab Control Microbiológico S.L. for their susceptibility evaluation. Table II shows a summary of the final bacterial counts from each sample after the incubation period, both in the control samples (without Bactiblock ) and in the samples in which the additive was incorporated. Also, the means and the R values are indicated. Table II. Antimicrobial capacity of the panels against the growth of E. coli after 24 h at 37ºC. Sample CFU/mL CFU/mL CFU/mL Mean R Control 2,8E+8 2,8E+8 Panel +,5% Brand A,3E+8,4E+8,E+8,27E+8,34 Panel + 3% BB R.43 2,E+8,8E+8 2,2E+8 2,E+8,5 Panel +,5 % BB S.9 6,8E+5,5E+3,3E+5 2,7E+5 3, Conclusions Test samples with Brand A or Bactiblock R.43 did not show remarkable antimicrobial properties at the used load levels (.5% and 3%, respectively). On the other hand, the samples with.5% of Bactiblock S.9 were able to reduce the final bacterial counts in approximately 3 log units, which correspond to a bacterial death of 99.9%. 6

19 . Summary Table Product Grade Physical state Matrix Active ingredient Other ingredients Dispersion Antimicrobial effectiveness BactiBlock R.43 Powder Bentonite Silver (,5%) N/A BactiBlock S.9 Powder Bentonite Silver (3%) N/A Powder Solube Glass Silver (,8%),,6 en TDS Mg, Al, fosfato Powder Solube Glass Silver (2%),,8 en TDS Silver-magnesiumcalcium-phosphateborate Brand F Masterbach Zirconium phosphate Silver (,23%) N/A Brand G Liquid Water Silver (,24%) N/A na + Brand A Powder Zeolite Silver (,3-2,5%), Zinc (o,-4,4%), ammonium (,-2,5%) N/A Brand B Powder Zeolite 62% of Zn and,3% of silver N/A + + Brand E Powder Silver salts Silver (,8%) N/A