Instructor: Dr. Kelly Cude. Course Syllabus

Transcription

1 BIO 221 Spring 2015 Instructor: Dr. Kelly Cude Course Syllabus Course Meeting Times T, TH 12:50-2:10pm lecture T, TH 2:30-5:35pm lab Instructor Office Hours M: 11:30-1:00 pm BONH 319 T: 11:00-12:30 pm BONH 319 by Prerequisite: Biological Science 107, or equivalent, with a minimum grade of C. College Chemistry recommended (Chemistry 151 or equivalent). Student Learning Outcomes (SLOs): Lecture: 1. Compare and contrast the major groups of microorganisms with regards to morphology, anatomy, physiology, classification, identification, disease and control and explain the ubiquity of microbes and their importance in human affairs. Laboratory: 1. Demonstrate mastery of aseptic technique, microbiological procedures, and laboratory safety. 2. Interpret results and data of exercises in microbial physiology and identification. Textbooks and materials: 1. Lecture Textbook: Prescott, Microbiology, 9th Edition, McGraw-Hill. 2. Laboratory Book: Leboffe/Pierce, Microbiology: Laboratory Theory and Application (Custom from COC bookstore only) 3. Lab coat (may rent one from the Biology Club $6) 4. Box of Glass slides (approx. 72) 5. Safety Glasses for lab (can buy cheaply at Home Depot or Harbor Freight) 6. Two bluebooks, 4 scantrons #882 (50 question/side) Course Blackboard The log in site is: Login: 7 DIGIT STUDENT ID Password: student (unless you have changed it)

2 Assignments & Assessment 4 Lecture Exams (100 points each) 400 points Daily Lab Completion points 90 points Lab Notebook (6 x 10 points) 60 points Lab Quizzes (6 x 15 points) 90 points Unknown #1 60 points Unknown #2 70 points Lab Final Exam (cumulative) 100 points Total 870 points Grading Scale: % A 80-89% B 70-79% C 60-69% D Below 59% F Lecture Assessment: Exams will cover lecture material and will consist of short answer questions. Lecture outlines and review guides will be posted to Blackboard to aid in studying. You are responsible for all material covered in lecture and assigned reading in the textbook. BE AWARE: THERE ARE NO MAKEUP EXAMS***. Lab Assessment: Read lab materials BEFORE coming to lab and BE ON TIME! Quizzes will be given at the start of lab periods and will cover material from the previous labs as indicated on your lab schedule. I will check off your lab notebook on the days we have quizzes including any and all assigned lab data sheets for the material covered on each quiz. Always keep your notebook up to date! In the last half of the semester, you will receive two separate unknown samples. Proper identification of the samples is worth 60 points (UNK #1) and 70 points (UNK#2) respectively and will include a summary of the techniques used in the identification process (blue book). The lab final will be a cumulative practical and consist of fill-in short answer questions. BE AWARE: THERE ARE NO MAKEUP LAB SESSIONS OR LAB QUIZZES***. *** In cases of extreme illness, family emergency/death,or jury duty, students may contact instructor regarding a makeup exam or lab quiz. If a makeup exam or quiz is warranted (per instructor s discretion), the instructor will require proper documentation (doctor s note, lawyer s note, death certificate ect). In addition, requests for makeup exams or quizzes must be made by 5:30pm on the day of the missed assignment. Plagiarism: COC has a zero tolerance policy on cheating. Any student caught cheating will immediately receive a failing grade (F) for the assignment and be referred to the dean of students (can lead to expulsion). Cheating includes talking during the exam, passing notes during an exam, coping from another student s exam or work, producing unoriginal work (ie copying directly from the text w/o citing), using cheat sheets, cell phones, ipods, or other unauthorized written or verbal sources on an exam.

3 Important Degree Information Check out the Math-Sciences Division website to see which classes are required for various degree programs and/or to see if you qualify to graduate with one of these degrees: Additional Resources MATHEMATICS, ENGINEERING, SCIENCE ACHIEVEMENT (MESA -ALLB 114): MESA is a program that supports students to excel in math, engineering, and the sciences. MESA is based on a rigorous program that builds academic skills and encourages cooperative solving. There is access to technology, academic advisement, and other support services are available. For more information, please contact the MESA Program Director in ALLB-114 or call (661) DISABLED STUDENTS SERVICES (DSPS): COC provides services for students with disabilities in compliance with the American Disabilities Act (ADA) and Section 504 of the Rehabilitation Act of If you need special accommodations please see me early in the semester. The DSP&S Office is located in Seco Hall, room 103 (behind financial aid). Phone Number: (661) ; Telephone Devise for the Deaf (TTY): (661) THE LEARNING CENTER: For tutoring in English, Math, Science, and other subjects on a walk-in basis, please visit the state-of-the-art TLC. Call for hours of operation at If you are having trouble--contact me, form a study group or study with a friend, and use the TLC services. Seek help as soon as you need it, the college has many resources, don t wait until it s too late! IMPORTANT DATES: Drop Deadline (no W): February 22, 2015 Withdrawal Deadline (W): May 8, 2015 Last Day of Class: June 4, 2015

4 Note: schedule is subject to change at discretion of instructor Section 1: February 10th March 5th First Section Topics Chapter 2/10-2/12 Introduction to Microbiology 1 - history of microbiology, 3 domains of life - current microbiology applications ** Eukaryotic Cell Structure (on your own!) 5** - organelle functions - phagocytosis, endocytosis 2/17-2/19 Protozoa and Protozoan Diseases 25, 40 - What are protists? Protist motility - Mechanisms for: osmoregulation, conjugation - Methods of transmission/types of infections 2/24 Fungi and Fungal Diseases 26, 40 - Basidiomycota, Zygomycota, Ascomycota - sexual and asexual lifecycles - Methods of transmission/types of infections 2/26-3/3 Viruses and Viral Diseases 6, 38 - viral structure/morphologies - bacteriophages - lytic vs. lysogenic lifecycles - virus v. viroid - example viruses (flu/hiv replication cycles) THURS 3/5 *** EXAM 1 ***

5 Section 2: March 10th April 2nd Second Section Topics Chapter 3/10 3/12 Prokaryotic Structure Function 3, 4 - prokaryotic organization/morphologies - Bacteria vs. Archaea - cell wall compositions - cell motility 3/17-3/24 Microbial Nutrition and Growth 7, 11 - culture media/ types of media - nutrition requirements/ growth curves - microbial function in the environment - biofilms 3/26-3/31 Microbial Control 8 - conditions affecting microbial control - antisepsis, disinfection, sanitize, -cide -static - chemical vs. physical methods THURS 4/2 *** EXAM 2 *** April 6-12 *** Spring Break *** "Clip art from the Clip Art Gallery on DiscoverySchool.com"

6 Section 3: April 14th May 13th Section 3 Topics Chapter 4/14-4/16 Microbial Evolution & Genetics 13( ) - Bacterial reproduction strategies 14 ( ) - Horizontal gene transfer 16, 18 - Plasmids, transposons - Operons - Mutation vs. recombination 4/21-4/28 Taxonomy & Archaea 19, 20 - Classification, Taxonomy - Nomenclature - Systematic vs Determinative - Bergy s Manuals 4/30 5/5 Bacterial Classification Cyanobacteria, - Chlamydiae - Spirochaetes - Proteobacteria - Actinobacteria - Firmicutes THURS 5/7 *** EXAM 3 ***

7 Section 4: May 12th June 4th Section 4 Topics Chapter 5/12-5/14 Pathogenicity & Epidemiology 35 & 37 - virulence, cycle of disease - recognition and surveillance - cases, controls 5/19-5/21 Bacterial Diseases 39 - major diseases caused by bacteria - methods of transmission/cycle of disease - emerging/re-emerging diseases - bioterrorism - historical significance 5/26 Antimicrobial Chemotherapy 9 - history of development - antibiotics (mechanisms) - penicillin - antibiotic resistance 5/28-6/2 Immunology 33, 34 - Nonspecific (Innate) vs. Specific (Acquired) - Antigens, Antibodies, White Blood Cells, lymphocytes - Types of antibodies: IgG, IgA, IgD, IgE, IgM - Recognition of foreign - Immune disorders Thurs 6/4 ***EXAM 4 ***

8 Schedule of LAB Assignments, Quizzes & Notebook Checks FEB 17 th Quiz 1 (15 pt) & Notebook Check 1 (10 pt) Microscopy /Ocular Micrometers Protozoa FEB 24 th Quiz 2 (15 pt) & Notebook Check 2 (10 pt) Helminthes Fungi MAR 10 th Quiz 3 (15 pt) & Notebook Check 3 (10 pt) Culture/Media Prep Bacterial Population Counts (Dilution Factors) Bacteriophage Titer MAR 17 th Quiz 4 (15 pt) & Notebook Check 4 (10 pt) Microbial Growth (Temp, ph, Osmotic Pressure) Germicidal UV Antibiotics Antiseptics/Disinfectants MAR 26 th Quiz 5 (15 pt) & Notebook Check 5 (10 pt) Simple, Negative, & Gram Staining Spore & Acid Fast Staining APR 16 th Quiz 6 (15 pt) & Notebook Check 6 (10 pt) Physiological Tests Differential Medias Culture Characteristics APR 28 th Unknown #1 Bluebook Due (60 pt) MAY 14 th Unknown #2 Bluebook Due (70 pt) MAY 28 th Cumulative Lab Final (100 pt)

9 BIOL SCI 221 INTRODUCTION TO MICROBIOLOGY LABORATORY SCHEDULE Spring 2015 Tuesday/Thursday WK DATE DESCRIPTION EXERCISE 1 Feb. 10 Safety Introduction to Phase-Contrast Microscopes, Protozoa pp. 1-6, Ex. 3-1,3-3*,4*; 12-3, 3-12* 1 Feb. 12 Protozoa, Ocular Micrometer Ex.12-3,3-2, 3*, 4*,12* 2 Feb. 17 Helminths, Eaten Alive Ex QUIZ 1 2 Feb. 19 Fungi, Aseptic technique Ex. 12-1,3-3*,1-3 3 Feb. 24 Culture Media Preparation, Pure Culture Technique Ex. 1-2,3,4; 2-12* QUIZ 2 Start Building Stock Cultures 3 Feb. 26 Bacterial Population Counts Ex. 6-1, 6-3*, App. C &E, Handout 4 March 3 Bacteriophage Titer and Clearing, Sterile Ex. 6-5 Technique/Transfer Assessment 4 March 5 Temperature and Growth ph and Microbial Growth Osmotic Pressure and Microbial Growth Lethal Effect of Ultraviolet Light 5 March 10 QUIZ 3 Antimicrobial Sensitivity Testing Antiseptics: Evaluation of Antiseptics Use Dilution: Disinfectant Effectiveness (Finish Stocks) Ex. 2-9 Ex Ex Ex Ex. 7-3 Handout, p. 60* Ex March 12 Bacteria, Smear Prep, Simple and Negative Staining Ex. 3-5, 3-6, pp * 6 March 17 Gram Staining Ex. 3-7 QUIZ 4 6 March 19 Spore, Acid Fast and Capsule Staining Ex. 3-10, 3-8, 3-9* 7 March 24 Stock Culture and Culture Characteristics Review, Oxygen requirements, and Selective Media Ex. 2-1,2,3,4,7,8; 4-4,5,6, pp *, Physiological Handout 7 March 26 Physiological Characteristics: Fermentation/Utilization tests Ex. 5-3,4,5,6,8, pp * QUIZ 5 Chromogenic Demo 8 March 31 Physiological Characteristics: Hydrolytic/Differential tests Ex. 5-12,13,14, 15,21,25; Indole 8 April 2 Evaluations Section 5 April 7, 9 SPRING BREAK 9 April 14 Stain Review, Gram Stain Assessment Section 3 9 April 16 Unknown #1 QUIZ 6 10 April 21 Unknown #1 10 April 23 Unknown #1 11 April 28 Unknown #1 Due/ Start Unknown #2 11 April 30 Unknown #2 12 May 5 Unknown #2 12 May 7 Unknown #2 13 May 12 Unknown #2 13 May 14 Unknown #2 Due Miniaturized Multitest Systems 14 May 19 Bacterial Examination of Water, Milk, and Food Ex. 8-12; Handouts 14 May 21 Evaluations 15 May 26 Immunology & Laboratory Check-Out Handouts 15 May 28 LABORATORY FINAL EXAM * Assigned reading (not in- lab activity) Note: Daily lab completion = 3 points