DNA, RNA, and protein purification

Transcription

1 DNA, RNA, and protein purification Catalog supplement 2013

2 Contents MACHEREY-NAGEL Bioanalysis catalog supplement 2013 This Bioanalysis catalog supplement 2013 is an addition to the main Bioanalysis catalog 2011 / Introducing 11 new products and presenting 4 product updates, the catalog supplement 2013 shows all latest MACHEREY-NAGEL Bioanalysis developments at a glance. Contents Plasmid DNA Page Category Transfection-grade plasmid DNA NucleoBond Xtra BAC 2 NEW Transfection-grade, endotoxin-free plasmid DNA NucleoBond 96 Xtra EF 3 NEW MACHEREY-NAGEL Clean-up PCR clean-up and gel extraction NucleoSpin Gel and PCR Clean-up 4 Update PCR clean-up NucleoSpin 8 PCR Clean-up 5 Update NucleoSpin 96 PCR Clean-up 5 Update Genomic DNA clean-up NucleoSpin gdna Clean-up 7 NEW NucleoSpin gdna Clean-up XS 8 NEW RNA MicroRNA NucleoSpin mirna Plasma 9 NEW RNA from blood NucleoSpin RNA Blood 10 NEW NucleoSpin RNA Blood Midi 11 NEW NucleoSpin 8 RNA Blood 12 NEW NucleoSpin 96 RNA Blood 12 NEW Viral RNA and DNA Viral RNA and DNA from serum and plasma NucleoSpin Dx Virus 14 NEW Protein Purification of His-tag proteins Protino 96 Ni-NTA 15 NEW Auxiliary tools and transfer media Sterile filtration MN Sterilizer CA 16 Update

3 MACHEREY-NAGEL Product information Apart from the Bioanalysis catalog 2011 / 2012 and the new supplement 2013, more product information is outlined in diverse marketing materials. Product selection guide summary of the main specifications of all Bioanalysis products at a glance Product guides overview of certain product groups Product flyers presentation of single products Lab focus flyers selection of products relevant for certain laboratory focuses For additional information material, please contact MN (tech-bio@mn-net.com) or see /bioflyerscatalog. Constantly updated product information, application data, literature, and product news are provided at the MN website: /Bioanalysis. NEWS MACHEREY-NAGEL introduces the new Bioanalysis icons. Each product chapter is now represented by a new icon. The icons, in addition to the different colors of the chapters, allow a fast orientation to easily find the products of interest. The new icons will be shown in product information, product material, and marketing material. Plasmid DNA Clean-up RNA MN quality Genomic DNA Viral RNA and DNA Protein High throughput Accessories Auxiliary tools EN ISO certification MACHEREY-NAGEL has been certified under the EN ISO 9001 norm since 1996 and for medical devices under the EN ISO norm since Selected products are CE-certified in accordance with EU Directive 98/79/EC. Sophisticated German manufacturing and strict quality control lay the foundation for high quality standards that our products consistently meet. This quality is ensured by the corresponding MACHEREY-NAGEL seal present on our products. MACHEREY-NAGEL Made in Germany Certified Quality MACHEREY-NAGEL Forensic products Consumables used in forensics need to be specially treated in order to deactivate any PCR-amplifiable DNA. MACHEREY-NAGEL has a stringent and controlled production process to avoid any contaminating PCR-detectable background. MN products carrying the forensic quality seal contain plastic materials that are ethylene oxide treated to inactivate any DNA. 1

4 Transfection-grade plasmid DNA transfection-grade endotoxin-free high- / low-copy plasmids large constructs desalting tool NEW NucleoBond Xtra BAC The 2 nd generation of anion exchanger for large construct plasmid DNA Optimized column design one prep in approximately 75 min Column filter included parallel lysate clearing and loading onto the column Optimal silica material yields up to 150 μg Transfection-grade plasmid DNA using proven anion-exchange technology LyseControl for visualization of completed lysis / neutralization Anion-exchange chromatography Gravity-flow columns Lysate clarification Column filters ml Vector size < 300 kbp Typical yield μg A 260 /A Isopropanol precipitation Centrifugation Preparation time 75 min/prep Binding capacity 150 μg Plasmid DNA Applications Purification of P1, BACs, PACs, and other large constructs from E. coli Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection For detailed product information and application data, see /plasmid NucleoBond Xtra BAC 10 NucleoBond Xtra BAC Columns with inserted NucleoBond Xtra Column Filters, buffers, RNase A 25 as above Product accessories Pack of Specification REF NucleoBond Xtra Combi Rack 1 for use with NucleoBond Xtra Midi, Maxi, BAC, AX 100, 500, 2 000, and Columns NucleoBond Xtra BAC Buffer Set 1 additional lysis buffers (1 L each): Buffers RES-BAC, LYS-BAC, NEU-BAC, and RNase A

5 Transfection-grade, endotoxin-free plasmid DNA transfection-grade endotoxin-free high-copy plasmids NEW NucleoBond 96 Xtra EF High-throughput isolation of endotoxin-free plasmid DNA in 96-well format Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells Patented endotoxin removal by additional washing step Processing of up to 5 ml bacterial culture NucleoBond Filter Plate for convenient filtration of bacterial lysates Processing under vacuum or by centrifugation Innovative MN Wash Plate minimizes risk of cross-contamination NucleoBond Finalizer Plate to speed up DNA precipitation Anion-exchange chromatography 96-well plates Processing Manual Lysate clarification 96-well filter plates 1 5 ml E. coli culture Vector size < 15 kbp < 300 kbp (without NucleoBond Finalizer Plate) Typical yield 2 4 μg (1.5 ml LB / TB in 96-well culture plates) μg (5 ml LB / TB in glass tubes) A 260 /A Elution volume μl Preparation time 120 min/plate Binding capacity 50 μg Applications Manual isolation of high-copy plasmids Typical downstream applications: transfection, cloning, sequencing, PCR, restriction analysis Plasmid DNA For detailed product information and application data, see /plasmid NucleoBond 96 Xtra EF 1 x 96 NucleoBond Xtra EF Plate, NucleoBond Finalizer Plate, NucleoBond Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A, 4 x 96 as above Product accessories Pack of Specification REF NucleoVac Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum

6 PCR clean-up and gel extraction manual HTP automated HTP mini spin columns silica matrix Update NucleoSpin Gel and PCR Clean-up Formerly known as NucleoSpin Extract II PCR clean-up and gel extraction the two-in-one kit with optimized recovery and elution volume Two applications, one kit one buffer with optimal performance for both applications High recoveries for fragments down to 50 bp Minimized elution volume: 15 μl highly concentrated DNA Binding buffer with ph indicator Separate buffers for single stranded DNA or SDS containing samples available Suitable for all gel buffer systems (e.g., TAE, TBE) Clean-up Silica-membrane technology Mini spin columns < 400 μl PCR reaction mixture < 400 mg TAE / TBE agarose gel Fragment size 50 bp approx. 20 kbp Typical recovery % A 260 /A Elution volume μl Preparation time 10 min/6 preps Binding capacity 25 μg Applications Purification of PCR products Extraction of DNA from agarose gels Support protocol for DNA extraction from polyacrylamide gels Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see ) Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see ) Typical downstream applications: cloning, sequencing, PCR, restriction analysis For detailed product information and application data, see /cleanup NucleoSpin Gel and PCR Clean-up 10 NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes (2 ml), buffers 50 as above as above Product accessories Pack of Specification REF Buffer NTB 150 ml for clean-up of SDS-containing samples Buffer NTC 100 ml for clean-up of single stranded DNA

7 PCR clean-up Update manual HTP automated HTP 8-well strips 96-well plates 96-well systems NucleoSpin 8 / 96 PCR Clean-up NucleoSpin 8 / 96 PCR Clean-up Core Kit Formerly known as NucleoSpin 8 / 96 Extract II Clean-up of PCR products in flexible 8-well strip format and for high throughput in convenient 96-well plate format Time-saving parallel clean-up of PCR products Complete removal of primers and primer-dimers Optimal recovery Suitable for processing under vacuum and centrifugation Innovative MN Wash Plate minimizes risk of cross-contamination Suitable for manual or automated use NucleoSpin 8 / 96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms. Additional accessories can be combined as needed. NucleoSpin 8 PCR Clean-up NucleoSpin 8 PCR Clean-up Core Kit NucleoSpin 96 PCR Clean-up NucleoSpin 96 PCR Clean-up Core Kit Silica-membrane technology 8-well strips 96-well plates Processing Manual or automated, vacuum or centrifugation < 100 μl PCR reaction mixture Fragment size 50 bp 10 kbp 50 bp 10 kbp Typical recovery % % A 260 /A Elution volume μl μl Preparation time 30 min/6 strips 45 min/plate Binding capacity 15 μg 15 μg Clean-up Applications Manual or automated purification of PCR products For detailed product information and application data, see /cleanup, see next page 5

8 PCR clean-up manual HTP automated HTP 8-well strips 96-well plates 96-well systems NucleoSpin 8 PCR Clean-up 12 x 8 NucleoSpin PCR Clean-up Binding Strips, MN Wash Plate, Rack of Tube Strips, buffers 60 x 8 as above NucleoSpin 8 PCR Clean-up 48 x 8 NucleoSpin PCR Clean-up Binding Strips, buffers Core Kit NucleoSpin 96 PCR Clean-up 1 x 96 NucleoSpin PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers 2 x 96 as above x 96 as above x 96 as above NucleoSpin 96 PCR Clean-up Core Kit 4 x 96 NucleoSpin PCR Clean-up Binding Plates, buffers Product accessories Pack of Specification REF NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum Starter Set A 1 for use of NucleoSpin 8-well strips on the NucleoVac Vacuum Manifold Starter Set C 1 for use of NucleoSpin 8-well strips under centrifugation Clean-up 6

9 Genomic DNA clean-up mini spin columns mini spin columns XS design NEW NucleoSpin gdna Clean-up Post clean-up and concentration of large DNA fragments Effective post clean-up and concentration of large DNA fragments for successful downstream applications High recovery rates up to 90 % for DNA fragments 100 bp 50 kbp Concentrate your DNA sample easily and faster compared to microdialysis filtrations units 15 min/10 preps time-saving procedure Silica-membrane technology Mini spin columns < 150 μl aqueous DNA solution (< 25 μg DNA) Fragment size 100 bp approx. 50 kbp Typical recovery % A 260 /A Elution volume μl Preparation time 15 min/10 preps Binding capacity 50 μg Applications Clean-up and concentration of pre-purified DNA (organic extractions) Clean-up and concentration of DNA from enzymatic reactions Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin Gel and PCR Clean-up (see page 4) Typical downstream applications: qpcr, enzymatic reactions, Southern blotting, STR amplification Clean-up For detailed product information and application data, see /cleanup NucleoSpin gdna Clean-up 10 NucleoSpin gdna Clean-up Columns, Collection Tubes (2 ml), buffers 50 as above as above

10 Genomic DNA clean-up mini spin columns mini spin columns XS design NEW NucleoSpin gdna Clean-up XS Post clean-up and concentration of small amounts of large DNA fragments Efficient post clean-up of large DNA fragments for successful downstream applications Recommended for small DNA quantities extracted from, e.g., forensic samples Unique XS spin column for low elution volume (> 6 μl) highly concentrated DNA Easier and faster than microdialysis filtration units 20 min/6 preps time-saving procedure Silica-membrane technology Mini spin columns XS design < 400 μl aqueous DNA solution (< 2 μg DNA) Fragment size 100 bp approx. 50 kbp Typical recovery % A 260 /A Elution volume 6 10 μl Preparation time 20 min/6 preps Binding capacity 3 μg Clean-up Applications Clean-up and concentration of pre-purified DNA (organic extractions) Clean-up and concentration of DNA from enzymatic reactions Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin Gel and PCR Clean-up (see page 4) Typical downstream applications: PCR, enzymatic reactions, Southern blotting, qpcr, STR amplification For detailed product information and application data, see /cleanup NucleoSpin gdna Clean-up XS 10 NucleoSpin gdna Clean-up XS Columns, Collection Tubes (2 ml), buffers 50 as above as above

11 MicroRNA mini spin columns NEW NucleoSpin mirna Plasma Isolation of small RNA and DNA from plasma and serum Efficient and selective isolation of small RNA from plasma or serum samples Superior recovery of small RNA > 15 nt Optional on-column DNA digest for increased sensitivity in downstream applications Simple and fast procedure without phenol / chloroform Fragment size Elution volume Preparation time Binding capacity Silica-membrane technology Mini spin columns 300 μl plasma or serum (up to 900 μl with multiple loading) < 1000 nt / bp μl 40 min/10 preps (without rdnase digestion) 70 min/10 preps (with rdnase digestion) 200 μg Applications* Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters) Typical downstream applications: real-time qrt-pcr, chip hybridization * Patent pending; kits to be used for research purposes only (see page 17) For detailed product information and application data, see /RNA RNA NucleoSpin mirna Plasma 10 NucleoSpin mirna Columns, Collection Tubes (2 ml), buffers 50 as above as above

12 RNA from blood manual HTP automated HTP mini spin columns midi spin columns NEW NucleoSpin RNA Blood Direct blood lysis no selective erythrocyte lysis Isolation of RNA from fresh or frozen whole blood (human or animal) Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 C complete processing at room temperature) Superior RNA yield and quality from up to 400 μl whole blood Efficient on-column DNA removal for increased sensitivity in downstream applications Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin) Silica-membrane technology Mini spin columns < 400 μl whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 1 8 μg (400 μl whole blood)* A 260 /A Elution volume μl Preparation time 55 min/6 preps Binding capacity 200 μg * RNA yield strongly depends on the leukocyte number in each individual blood sample. RNA Applications** Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin) Typical downstream applications: qrt-pcr, next generation sequencing, blotting, array technology ** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see /RNA NucleoSpin RNA Blood 10 NucleoSpin RNA Blood Columns with Collection Tubes, Collection Tubes (2 ml), buffers, RNase-free rdnase, Liquid Proteinase K 50 as above

13 RNA from blood manual HTP automated HTP mini spin columns midi spin columns NEW NucleoSpin RNA Blood Midi Direct blood lysis no selective erythrocyte lysis Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal) Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 C complete processing at room temperature) Superior RNA yield and quality from up to 1.3 ml whole blood Efficient on-column DNA removal for an increased sensitivity in downstream applications Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin) Silica-membrane technology Midi spin columns μl whole blood (fresh or frozen) Fragment size > 200 nt Typical yield 4 26 μg (1300 μl whole blood)* A 260 /A Elution volume μl Preparation time 75 min/6 preps Binding capacity 700 μg * RNA yield strongly depends on the leukocyte number in each individual blood sample. Applications** Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin) Typical downstream applications: qrt-pcr, next generation sequencing, blotting, array technology RNA ** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see /RNA NucleoSpin RNA Blood Midi 20 NucleoSpin RNA Blood Midi Columns with Collection Tubes, Collection Tubes (15 ml), buffers, RNase-free rdnase, Liquid Proteinase K 11

14 RNA from blood NEW manual HTP automated HTP 8-well strips 96-well plates NucleoSpin 8 / 96 RNA Blood Direct blood lysis no selective erythrocyte lysis Isolation of total RNA from whole blood (human or animal) in flexible 8-well strip format or proven 96-well format Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 C complete processing at room temperature) Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin) Suitable for fresh and frozen blood samples rdnase included for efficient removal of genomic DNA Processing under vacuum or by centrifugation Suitable for manual and automated processing Innovative MN Wash Plate minimizes risk of cross-contamination RNA NucleoSpin 8 RNA Blood NucleoSpin 96 RNA Blood Silica-membrane technology 8-well strips 96-well plates Processing Manual or automated, vacuum or centrifugation < 400 μl whole blood (fresh or frozen) Fragment size > 200 nt > 200 nt Typical yield 1 8 μg 400 μl (whole blood)* 1 8 μg 400 μl (whole blood)* A 260 /A Elution volume μl μl Preparation time 60 min/6 strips 100 min/plate Binding capacity 100 μg 100 μg * RNA yield strongly depends on the leukocyte number in each individual blood sample. Applications** Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin Typical downstream applications: qrt-pcr, next generation sequencing, blotting, array technology ** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see /RNA 12

15 RNA from blood manual HTP automated HTP 8-well strips 96-well plates NucleoSpin 8 RNA Blood 12 x 8 NucleoSpin RNA Blood Binding Strips, MN Wash Plates, Square-well Blocks, Racks of Tube Strips, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rdnase, Liquid Proteinase K 60 x 8 as above NucleoSpin 96 RNA Blood 2 x 96 NucleoSpin RNA Blood Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rdnase, Liquid Proteinase K 4 x 96 as above Product accessories Pack of Specification REF NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum Starter Set A 1 for use of NucleoSpin 8-well strips on the NucleoVac Vacuum Manifold Starter Set C 1 for use of NucleoSpin 8-well strips under centrifugation RNA 13

16 Viral RNA and DNA from serum and plasma manual HTP automated HTP mini spin columns mini spin columns, CE-marked funnel columns NEW NucleoSpin Dx Virus CE-IVD marked kit CE-IVD marked mini spin kit* for the isolation of viral RNA and DNA CE-marked in accordance with EU Directive 98/79/EC Fits into in-vitro diagnostic workflows Compatible with fresh or frozen human serum and plasma treated with EDTA or citrate Highly consistent viral RNA and DNA isolation for a broad range of viral titers Also suitable for animal samples * CE-IVD marked kit: not available in all countries, please inquire. Fragment size Elution volume Preparation time Binding capacity Silica-membrane technology Mini spin columns 150 μl plasma or serum (fresh or frozen, EDTA or citrate treated) 100 bp approx. 50 kbp 50 μl 30 min/6 preps 40 μg Viral RNA and DNA Applications Isolation of viral RNA from 150 μl human plasma or serum Isolation of viral DNA from 150 μl human plasma or serum Isolation of viral RNA and viral DNA from 150 μl human plasma or serum (simultaneous isolation) Isolation of viral RNA and / or viral DNA from animal samples, swabs, plasma, or serum (out of the scope of the IVD Directive 98/79/EC). Typical downstream applications: enzymatic amplification and detection of viral RNA and / or viral DNA (e.g., PCR, RT-PCR) For detailed product information and application data, see /virus NucleoSpin Dx Virus 50 NucleoSpin Dx Virus Columns with Collection Tubes, Lysis Tubes (1.5 ml), Collection Tubes (2 ml), Elution Tubes (1.5 ml), buffers, Proteinase K, Carrier RNA

17 Purification of His-tag proteins NEW bulk material manual HTP automated HTP 96-well plates Protino 96 Ni-NTA High-throughput purification of His-tag proteins Efficient protein purification using chelating group NTA (nitrilotriacetic acid) Unique Protino Purification Plate for leak-free incubation during all steps Plate outlet and MN Wash Plate prevent cross-contamination High well-to-well reproducibility High capacity and high affinity Purification under native and denaturing conditions Processing under vacuum or by centrifugation IMAC (immobilized metal ion affinity chromatography) Chelating ligand NTA (nitrilotriacetic acid) 96-well plates (Protino Purification Plate with special filter frit) Matrix 6 % beaded agarose (cross-linked), precharged with Ni 2+, Volume capacity per well 1.4 ml Recommended sample volume < 750 μl/well, supplied matrix is sufficient for 96 preps with 50 μl of settled agarose beads per well Bed volume Variable (50 μl recommended) Binding capacity* < 2 mg per well when using 50 μl of settled agarose * Binding capacity will vary for each polyhistidine-tagged protein and strongly depends on chromatographic conditions. Applications Purification of polyhistidine-tagged proteins Batch binding experiments Screening experiments to optimize chromatographic conditions Protein For detailed product information and application data, see /protein Protino 96 Ni-NTA 1 x 96 Protino Ni-NTA Agarose, Protino Purification Plate, MN Wash Plate 4 x 96 as above Product accessories Pack of Specification REF Protino Purification Plate 1 96-well plate, suitable for centrifugation and vacuum as above Receiver Plate 10 μm 4 96-well plate with an inserted filter frit of 10 μm pore size, suitable for centrifugation and vacuum NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum MN Shaker Frame 1 shaking frame for Protino Purification Plate

18 Sterile filtration Update auxiliary tools blotting blood sample storage sterile filtration decontamination of EtBr surface protection MN Sterilizer CA Highest quality syringe filter for fast and efficient sterile filtration of aqueous solutions Removal of particulates > 0.2 μm Fast flow of liquid through the filter Very robust membrane high stability Low protein binding Auxiliary tools and transfer media Syringe filter units Housing material Methyl methacrylate-butadiene-styrene polymer (MBS) Membrane material Cellulose acetate Nominal pore size 0.2 μm Filter diameter 28 mm Effective filtration area 6.2 cm 2 Hold-up volume (after air purge) 150 μl Inlet connection Female luer lock Outlet connection Male slip luer Maximum pressure 4.5 bar Maximum temperature 50 C Sterilization method Pre-sterilized with ethylene oxide Applications Sterile filtration and removal of particulates from aqueous solutions For detailed product information, see /auxiliary Product Pack of Specification REF MN Sterilizer CA 50 Disposable filter units, membrane material CA (cellulose acetate), pore size 0.2 μm, membrane diameter 28 mm

19 Trademarks Product use restriction Trademarks used in this supplement MACHEREY-NAGEL trademarks NucleoBond NucleoSpin Protino kits and cartridges for nucleic acid purification (anion-exchange chromatography) kits for purification of DNA and RNA (silica-membrane technology) kits for purification of recombinant proteins (affinity chromatography) Trademarks of other companies Registered trademarks TRIzol Molecular Research Center, Inc. PCR is patented by Hoffmann La Roche Disclaimer All used names and denotations can be brands, trademarks, or registered labels of their respective owner also if they do not have a special denotation. To mention products and brands is only a kind of information, i.e., it does not offend against trademarks and brands and can not be seen as a kind of recommendation or assessment. Regarding these products or services we can not grant any guarantees regarding selection, efficiency, or operation. Product use restriction / Warranty MACHEREY-NAGEL Bioanalysis products and their single components are intended, developed, designed, and sold FOR RESEARCH PURPOSES ONLY, except, however, any other function of the product being expressly described in original MACHEREY-NAGEL product leaflets. MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY! MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY! MACHEREY-NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING. For detailed information please refer to the respective Material Safety Data Sheet of the product! MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT. MACHEREY-NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application. Application on the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all damages resulting from such application. DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO-USES ONLY! ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable for IN-VITRO-diagnostic use. Please pay attention to the package of the product. IN- VITRO-diagnostic products are expressly marked as IVD on the packaging. IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN-VITRO-DIAGNOSTIC USE! ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE (INCLUDING, BUT NOT LIMITED TO DIAGNOSTIC, THERAPEUTIC, AND/OR PROGNOSTIC USE). No claim or representations are intended for its use to identify any specific organism or for clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or blood banking). It is rather in the responsibility of the user or in any case of resale of the products in the responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification products of MACHEREY-NAGEL for a well-defined and specific application. MACHEREY-NAGEL shall only be responsible for the product specifications and the performance range of MN products according to the specifications of in-house quality control, product documentation, and marketing material. For detailed information on product use restriction and warranty please refer to the respective user manuals. Trademarks Product use restriction 17

20 DNA, RNA, and protein purification Catalog supplement 2013 Service Bioanalysis Technical Support and Customer Service phone +49 (0) Product Management phone +49 (0) (0) Sales and Marketing phone +49 (0) (0) Business Development phone +49 (0) Your local distributor KATEN Catalog Supplement en2/15/0/ PD Printed in Germany MACHEREY-NAGEL EN ISO 9001: 2008 CERTIFIED MACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str Düren Germany Germany and international: Switzerland: MACHEREY-NAGEL AG France: MACHEREY-NAGEL EURL Tel.: Tel.: Tel.: Fax: Fax: Fax: info@mn-net.com sales-ch@mn-net.com sales-fr@mn-net.com USA: MACHEREY-NAGEL Inc. Tel.: Fax: sales-us@mn-net.com