CDx XPRESS RAPID PATHOGEN DETECTION SYSTEM

Transcription

1 CDx XPRESS RAPID PATHOGEN DETECTION SYSTEM PRODUCT DESCRIPTION LIQUID CRYSTAL BIOSENSORS, the heart of the CDx rapid pathogen detection system is the first new food safety testing system with commercial application in the last 30 years. CDx Xpress relies on Liquid Crystal Technology to detect targeted pathogens. CDx Xpress is a faster, simpler, more reliable way of separating the signal from all the background noise, increasing speed of results, and sensitivity. Kit for E. coli O157 Antibody Kits Part # tests per kit Part # Liquid Crystal BioCassettes Part # tests per case Part # ACCURACY An independent, third party study of equipment performance determined that the CDx Xpress rate of false positives was less than 1%, the lowest of any commercial system. SPEED Time to result for initial samples in less than 11 hours, samples per hour thereafter. COST The CDx Xpress System up-front investment is typically less than half of a comparable PCR system, and all-in operating costs are lower as well. CDx is AOAC accredited for the rapid detection of E. coli O157 in Raw Ground Beef, Beef Trim and Spinach. Additional Antibody Kits and food applications available.

2 E. coli O157 SAMPLE PREPARATION Enrichment Beef 325 g of raw ground beef or beef trim and 1000 ml of preheated (46 C) mtsb+n+vcc in filtered stomacher bag. Hand massage for 30 sec., incubate at 42 C for hours. 09:35 Incubate and pour/pipette-off ml of enriched sample into 50 ml conical tube 09:45 (0:10) Allow enriched sample to settle Spinach 200 g of spinach and 600 ml of preheated (46 C) TSB+VCC in filtered stomacher bag. Hand massage for 30 sec., incubate at 42 C for hours. XPRESS E PROTOCOL 09:47 (0:02) Transfer 195 μl of antibody to deep well plate 09:49 (0:02) Transfer 1.4 ml of enriched sample to 1.5 ml microcentrifuge tube and centrifuge 10:04 (0:15) Add 300 μl of enriched sample to deep well plate with antibody and perform Immuno-Magnetic Separation 10:06 (0:02) Add 18 μl of sample and 90 μl of Liquid Crystal Reagent into 0.2 ml plate 10:08 (0:02) Move 50 μl of sample from 0.2 ml plate to each inlet port of BioCassette, draw sample to fill line, store in chiller 10:16 (0:08) Process each BioCassette (Negative and Target) to Reader For additional information or to place an order, contact: 10:17 (0:01) Review results displayed, printed or ed SS_EC 0217 Lit. No W

3 CDx XPRESS RAPID PATHOGEN DETECTION SYSTEM PRODUCT DESCRIPTION LIQUID CRYSTAL BIOSENSORS, the heart of the CDx rapid pathogen detection system is the first new food safety testing system with commercial application in the last 30 years. CDx Xpress relies on Liquid Crystal Technology to detect targeted pathogens. CDx Xpress is a faster, simpler, more reliable way of separating the signal from all the background noise, increasing speed of results, and sensitivity. STEC Kit for E. coli O157 and non-o157 Shiga toxin-producing E. coli (O26, O45, O103, O111, O121, O145) Antibody Kits Part # tests per kit Part # Liquid Crystal BioCassettes Part # tests per case Part # ACCURACY An independent, third party study of equipment performance determined that the CDx Xpress rate of false positives was less than 1%, the lowest of any commercial system. SPEED Time to result for initial samples in less than 11 hours, samples per hour thereafter. COST The CDx Xpress System up-front investment is typically less than half of a comparable PCR system, and all-in operating costs are lower as well. CDx is AOAC accredited for the rapid detection of E. coli O157 and non-o157 Shiga toxin-producing E. coli (STEC) in Raw Ground Beef, Beef Trim and Spinach. Additional Antibody Kits and food applications available.

4 STEC E. coli O157 and non-o157 Shiga toxin-producing E. coli (O26, O45, O103, O111, O121, O145) SAMPLE PREPARATION Enrichment Beef 325 g of raw ground beef or beef trim and 1000 ml of preheated (46 C) mtsb+n+vcc in filtered stomacher bag. Hand massage for 30 sec., incubate at 42 C for hours. 09:35 Incubate and pour/pipette-off ml of enriched sample into 50 ml conical tube 09:45 (0:10) Allow enriched sample to settle Spinach 200 g of spinach and 600 ml of preheated (46 C) TSB+VCC in filtered stomacher bag. Hand massage for 30 sec., incubate at 42 C for hours. XPRESS STEC PROTOCOL 09:47 (0:02) Transfer 195 μl of antibody to deep well plate 09:49 (0:02) Transfer 1.4 ml of enriched sample to 1.5 ml microcentrifuge tube and centrifuge 10:04 (0:15) Add 300 μl of enriched sample to deep well plate with antibody and perform Immuno-Magnetic Separation 10:06 (0:02) Add 18 μl of sample and 90 μl of Liquid Crystal Reagent into 0.2 ml plate 10:08 (0:02) Move 50 μl of sample from 0.2 ml plate to each inlet port of BioCassette, draw sample to fill line, store in chiller 10:32 (0:31) Process each BioCassette (Negative, Panel A, Panel B) to Reader For additional information or to place an order, contact: 10:33 (0:01) Review results displayed, printed or ed SS_STEC 0217 Lit. No W

5 CDx XPRESS RAPID PATHOGEN DETECTION SYSTEM PRODUCT DESCRIPTION LIQUID CRYSTAL BIOSENSORS, the heart of the CDx rapid pathogen detection system is the first new food safety testing system with commercial application in the last 30 years. CDx Xpress relies on Liquid Crystal Technology to detect targeted pathogens. CDx Xpress is a faster, simpler, more reliable way of separating the signal from all the background noise, increasing speed of results, and sensitivity. Kit for Listeria monocytogenes Antibody Kits Part # tests per kit Part # Liquid Crystal BioCassettes Part # tests per case Part # ACCURACY An independent, third party study of equipment performance determined that the CDx Xpress rate of false positives was less than 1%, the lowest of any commercial system. SPEED Time to result for initial samples in less than 28 hours, samples per hour thereafter. COST The CDx Xpress System up-front investment is typically less than half of a comparable PCR system, and all-in operating costs are lower as well. CDx is AOAC accredited for the rapid detection of Listeria monocytogenes from Environmental Surfaces. Additional Ab Kits, incubation times, and food applications available.

6 Listeria monocytogenes SAMPLE PREPARATION Enrichment Surface Swab Swab the intended surface using a spongestick or similar with neutralizing buffer. Return sponge to the bag and add 50 ml of appropriate preheated (42 C) media. Hand massage for 30 seconds, incubate at 30 C for hours. 00:01 Swab surface and return sponge to bag 27:02 (27:01) Add 50 ml of media, hand massage for 30 seconds and incubate XPRESS LM PROTOCOL 27:04 (0:02) Transfer 180 μl of antibody to deep well plate 27:05 (0:01) Transfer 1.3 ml of enriched sample to 1.5 ml microcentrifuge tube 27:21 (0:15) Add 300 μl of enriched sample to deep well plate with antibody and perform Immuno-Magnetic Separation 27:22 (0:02) Add 12 μl of sample and 60 μl of Liquid Crystal Reagent into 0.2 ml plate 27:24 (0:02) Move 50 μl of sample from 0.2 ml plate to each inlet port of BioCassette, draw sample to fill line, store in chiller 27:32 (0:08) Process each BioCassette from chiller (Negative and Target) to Reader For additional information or to place an order, contact: 27:33 (0:01) Review results displayed, printed or ed SS_LM 0217 Lit. No W

7 CDx XPRESS RAPID PATHOGEN DETECTION SYSTEM PRODUCT DESCRIPTION LIQUID CRYSTAL BIOSENSORS, the heart of the CDx rapid pathogen detection system is the first new food safety testing system with commercial application in the last 30 years. CDx Xpress relies on Liquid Crystal Technology to detect targeted pathogens. CDx Xpress is a faster, simpler, more reliable way of separating the signal from all the background noise, increasing speed of results, and sensitivity. Kit for Salmonella spp. Antibody Kits Part # tests per kit Part # Liquid Crystal BioCassettes Part # tests per case Part # ACCURACY An independent, third party study of equipment performance determined that the CDx Xpress rate of false positives was less than 1%, the lowest of any commercial system. SPEED Time to result for initial samples in less than 16 hours, samples per hour thereafter. COST The CDx Xpress System up-front investment is typically less than half of a comparable PCR system, and all-in operating costs are lower as well. CDx is AOAC accredited for the rapid detection of Salmonella spp. on Raw Whole Tomatoes and Whole Chicken Rinsate. Additional Ab Kits, incubation times, and food applications available.

8 Salmonella spp. SAMPLE PREPARATION Enrichment Whole Uncut Tomato One whole uncut tomato and 400 ml of appropriate preheated (46 C) media in filtered stomacher bag. Ensure all surfaces of the tomato have been exposed to the media, incubate at 42 C for 15 hours. 15:05 Incubate and pour/pipette-off ml of enriched sample into 50 ml conical tube 15:15 (0:10) Allow enriched sample to settle XPRESS S PROTOCOL Whole Chicken Wash One whole chicken carcass and 300 ml of Buffered Peptone Water in sterile vessel, shake for 1 minute and rinse. Add 30 ml aliquots of the BPW rinse to separate containers. Add 30 ml of enrichment media to each aliquot. Incubate at 41 C for 15 hours. 15:17 (0:02) Transfer 195 μl of antibody to deep well plate 15:19 (0:02) Transfer 1.4 ml of enriched sample to 1.5 ml microcentrifuge tube and centrifuge 15:34 (0:15) Add 300 μl of enriched sample to deep well plate with antibody and perform Immuno-Magnetic Separation 15:36 (0:02) Add 18 μl of sample and 90 μl of Liquid Crystal Reagent into 0.2 ml plate 15:38 (0:02) Move 50 μl of sample from 0.2 ml plate to each inlet port of BioCassette, draw sample to fill line, store in chiller 15:46 (0:08) Process each BioCassette from chiller (Negative and Target) to Reader For additional information or to place an order, contact: 15:47 (0:01) Review results displayed, printed or ed SS_SAL 0217 Lit. No W