Core Cell Culture Product Training

Transcription

1 Core Cell Culture Product Training

2 Cell Culture What is Cell Culture? Complex process of growing cells under controlled conditions in vitro (outside of the body) Way to simulate cell growth in vivo (in the body) without risk Advantages for understanding cellular characteristics, drug discovery Types of cells grown in culture Mammalian Cell Culture skin, cancer, stem, neuronal Plant Cell Culture Insect Cell Culture Bacteria & Yeast Cell Culture 2

3 EasYFlasks Application & Positioning Thermo Scientific Nunc EasYFlask Application Expansion and Growth of Cells Positioning Premium quality flasks with highest consistency with the extensively tested Thermo Scientific Nunclon Delta surface, increased efficiency with unique features and proven performance with thousands of publications with numerous cell types 3

4 Features / Benefit Feature Cell Culture surface release tested with 4 cell lines Benefit Consistent Nunclon Delta surface lot to lot for reproducible results Ergonomically designed 1/3 turn Cap Reduced wrist fatigue/strain and repetitive movement injuries Variety of surface options Nunclon Delta - Cell Culture) Suspension - Non-treated surface PDL & Collagen I improved attachment for fastidious cells Flexibility to select the best surface for your cells and application 4

5 Features / Benefit Feature Angled neck and wider opening Benefit Increased access (reach 99.9%) to the surface for improved cell recovery Angled side walls allow the entire surface to be visualized under a standard microscope Printed graduations Increased confidence while estimating confluency Increased efficiency when filling flasks Y indicator on Cap Easy identification if the cap is venting or closed 5

6 Triple Flasks Application & Positioning Thermo Scientific Nunc TripleFlask Application Expansion and Growth of Cells for Cell Mass in large throughput labs, drug discovery or production of cellular components or Therapies or scale up Positioning Premium quality multiple layer flasks with increased efficiency with 500cm 2 in the footprint of a 175cm 2 flask, highest consistency with the extensively tested Nunclon Delta surface and proven performance with thousands of publications with numerous cell types. First to market with multiple layer flasks with 20 years of experience 6

7 Features / Benefit Feature 3 growth surfaces (500cm 2 ) in the footprint of a 175cm 2 flask Benefit Increased efficiency by growing 3 times the number of cells Release tested with 4 cell lines Straight neck design Consistent surface lot to lot for reproducible results Improved stability when stacked in the incubator Barcoded with large code 128 Increases the production capacity on the TAP SelecT instruments 7

8 Dishes Application & Positioning Thermo Scientific Nunc Dishes Applications Growth & Expansion number of customers growing strictly in Dishes for expansion Characterization and Analysis Cloning & Selection - Isolating pure cell strains or selecting for certain characteristics Characterization - Understanding information like species and tissue origin, morphology and chromosome analysis Differentiation Making a less specialized cell become more specialized cell types Transfection - Changing of the cells by transferring new DNA Positioning Premium quality Dishes with highest consistency with the extensively tested Nunclon Delta surface, proven performance with thousands of publications with numerous cell types, and flexibility to choose the best surface for your cells with multiple options. 8

9 Features / Benefit Feature Cell Culture surface release tested with 4 cell lines Benefit Consistent Nunclon Delta surface lot to lot for reproducible results Variety of surface options Nunclon Delta - Cell Culture) Suspension - Non-treated UpCell Nonezymatic HydroCell & Low Cell Binding - Low cell binding surface Ziplock bags 100 & 150mm Dishes Flexibility to select the best surface for your cells and application Reduced risk of contamination ensure that dishes are keep clean if unused Gridded options in the 35 & 60mm Dishes Increase efficiency during cloning applications 9

10 Multidishes Application & Positioning Thermo Scientific Nunc Multidishes Application Characterization and Analysis Cloning & Selection - Isolating pure cell strains or selecting for certain characteristics Characterization - Understanding information like species and tissue origin, morphology and chromosome analysis Differentiation Making a less specialized cell become more specialized cell types Transfection - Changing of the cells by transferring new DNA Positioning Premium quality Multiwell Dishes with highest consistency with the extensively tested Nunclon Delta surface, proven performance with thousands of publications with numerous cell types, and flexibility to choose the best surface for your cells with multiple options. 10

11 Features / Benefit Feature Cell Culture surface release tested with 4 cell lines Variety of surface options Nunclon Delta - Cell Culture) Suspension - Non-treated UpCell Nonezymatic HydroCell & Low Cell Binding - Low cell binding surface PDL & Collagen I improved attachment for fastidious cells Nunclon Vita - Animal component-free surface for growth of human stem cells without coatings or feeder cells Benefit Consistent Nunclon Delta surface lot to lot for reproducible results Flexibility to select the best surface for different cell types and applications Increased flexibility to optimize your process Variety of formats and # of wells available 11

12 Serological Pipettes - Application & Positioning Thermo Scientific Nunc Serological pipettes The extended portfolio of serological pipettes features a full range of sizes and packaging options offering quality, accuracy and performance Application Standard product used through the entire laboratory workflow 12

13 Features / Benefit / Competitive Edge Feature Benefit Competitive Edge Sterility assurance level (SAL) of 10-6 Certified non-pyrogenic Bold clearly marked graduations Non-dripping tip for pipette precision Color-coded packaging and larger volumes have color-coded plugs Highest sterility for enduser Easy-to-read scale markings Ease in sorting and selecting the correct size End-user mind at rest Ease of use when pipetting Convenient and easy recognition of sizes 13

14 15 & 50ml Conical Tube - Positioning Thermo Scientific Nunc Conical Tubes Premium, high quality conical tubes that are environmentally friendly, offers the highest cleanliness with a recyclable, plastic rack, and allows for increased traceability with the largest writing area on the market 14

15 Cell Culture Workflow Applications General Purpose - throughout workflow Dilutions and preparing Aliquots Storage of media components, buffers and solutions Freezing of media or samples in -80 C Centrifugation often in Benchtop Centrifuges Process RCF xg Time Temp Viable Cell Separation during Harvesting xg 10 min RT Post Thaw Washing of Frozen Cells 100xg 2 min RT Preparation of Conditioned Media 1000xg 10 min RT 15

16 Features / Benefit Feature Molded 2 Piece Plastic Rack System Benefit Recyclable Does not shed particulates Collapsible to reduce footprint to make it easier to recycle or store 16

17 Features / Benefit / Competitive Edge Feature Benefit Competitive Edge Largest Writing Area Rack with Labeling Area Additional space to label information for improved traceability Easy Identification Improved traceability for end-user 17

18 Features / Benefit Feature Higher RCF rating than former products Benefit Enables greater range of applications from cell to sub-cellular pelleting 18

19 EZ Flip15 & 50ml Conical Tube - Positioning Thermo Scientific Nunc EZ Flip Conical Tubes Premium, high quality conical tubes that offer reduced wrist fatigue & injuries with the ergonomic design - open handed opening and closing and reduced risk contamination with integrated cap. 19

20 Features / Benefit Ergonomically design flip top cap Reduced wrist fatigue/strain and repetitive movement injuries Integrated tube & hinged cap Reduces the risk of contamination 20

21 Summary Thermo Scientific Nunc has 60-years experience in developing dependable and quality cell culture products. The Nunclon Delta is a proprietary cell culture surface available in many different formats and surfaces. Full range of products for the whole cell culture workflow. Thermo Scientific Nunc offers a wide range of surfaces in various formats. 21

22 Question 1 What is a typical indicator that a laboratory is working with cell culture? A. 96 well plates everywhere B. Staff is wearing white coats and gloves C. various types of pipettes all over the place D. CO 2 incubator 22

23 Question 2 The key features of the Nunc EasYFlask are: A. made of virgin polypropylene and optically transparent B. wide neck and 1/3 turn to open cap C. sterility level SAL 10-3 and mycoplasma free D. molded volume graduations and ½ turn to open cap 23

24 Question 3 Which Nunc TripleFlask statement is not true? A. 500 cm 2 growth surface B. cell harvesting with cell scraper C. medium equilibration in upright position D. available with filter or vent/closed cap 24

25 Question 4 Nunc conical centrifuge tubes are the best choice because they: A. are made of robust Polycarbonate B. can be used for ultracentrifugation C. are QC tested with 4 different cell lines D. come in a recyclable plastic rack minimizing contamination risk 25

26 Question 5 Which core cell culture products are used all over the entire cell culture workflow? A. Tissue culture flasks B. MicroWell plates C. Serological pipettes D. Filter Units 26