Construction of an Integrated Database on Biosafety and use of GMOs in India

Transcription

1 FINAL TECHNICAL REPORT Construction of an Integrated Database on Biosafety and use of GMOs in India Principal Investigator : Dr. T. R. Sharma Coordinator : Prof. K.R. Koundal Co- Investigators: Dr. K. C. Bansal Dr. N. K. Singh Dr. Kishore Gaikwad National Research Center on Plant Biotechnology IARI, New Delhi trsharma@nrcpb.org

2 Objectives of the Project Construction and Updation of the Database Retrieval, storage and management of data on biosafety of GMOs To make GM Crops database public through a dedicated Web site To impart training to the Scientists and Technical Staff on Biosafety web resources

3 Relational Diagram Showing Relationship Among Different Tables

4 Basic Flow Chart of the NRCPB Database Enter query Authentication COMPUTE-SERVER (SUN V240) Results Query QUERY INTERFACE JAVA - JDBC Outputs WEB-SERVER (SUN ULTRA10) Inputs Primary Database (GM_Pub) MySQL Primary Database (Transgenics) MySQL MIDDLE WARE USER INTERFACE Gene Promo ter Crop Env_ haz Health_ haz Soil_ micr Develo -per APPL-SERVER JSP ENGINE HTML DHTML - FLASH MySQL

5 GM Crop Database

6 Availability of Database on WWW Click here to access GM crop database

7 GM Crop Database Home Page

8 GM Crop Search Database Interface

9 GM Crop Search Database Interface

10 Transgenic Line Query Interface

11 Transgenic Line Query Interface

12 Result Page for the Query Submitted

13 Transgene-Crop-Promoter Query Interface

14 Result Page for the Query Submitted

15 Promoter Info Query Interface

16 Result Page for the Query Submitted

17 Developer Institution Query Interface

18 Result Page for the Query Submitted

19 Crop Current status of information stored in transgenics database Number of transgenic line Number of genes Number of promoter Developer Cotton 137* 4** 1*** 20 Rice 4^ 5^^ 2^^^ 1 * Brinjal - 1 % 1*** 1 * Tomato - 1 # 1*** 1 * Potato - 2 & 2 && 1 * Chickpea *** 1 * Corn - 3^^^^ - - * 58 Bt cotton hybrids approved for commercial planting in India till date, rest are released for field trials in different zones. ** (cry1ac,cry1ab,cry2ab,vip3a), *** (CaMV 35S) ^ (SGR1, SGR2, LLRICE06, LLRICE62), ^^ (Psy, crti, lyc, Zea mays psy, Bar) ^^^ (Ubi-1 promoter, GT1 promoter), ^^^^ (cry1f,cry1x, CP4EPSPS) # (Rep gene) & (Ama1, RGA2), && ( CaMV 35S, GBSS) + (Cry1Aa3), % (Cry1Ab) * Transgenic crops under development and field trials

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21 GM Publication Query Interface

22 Result Page for the Query Submitted

23 Result Page for the Query Submitted

24 Result Page for the Query Submitted

25 Current status of information stored in GM-Publication Database- June 2007 Year Biosaf ety & IPR Issues Food & Feed Risks & Benefit s Environ ment & Health Concern Bt Cotton Rice Soy bean Brinjal Tomato Corn Potato Canola Soil Micro flora Select able Others Marker Total Total number of records = 788

26 List of Important Users of DB Total Number of Hits in the Database = Indian National Agricultural Research Research Centre on Institute Plant Biotechnology 3. Mahatma Phule Krishi Vidyapeeth 4. National Bureau of Plant Genetic Resources 5. Tata Energy Research Institute, TERI 6. NRC for Sorghum 7. Jawaharlal Nehru Krishi Vishwa Vidyalaya 8. Tamilnadu Agricultural University 9. Central Food Technological Research Institute 10. Israel Life Science Industry 11. Vasantdada Sugar Institute, Pune 12. Centre for DNA Fingerprinting and Diagnostics 13. Kerala Agricultural University 14. Cornell University Central Dr. Babasaheb Rice Research Ambedkar Institute Marathwada University 17. Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth 18. Rajasthan Agricultural University, Bikaner 19. Dhaka University 20. Reuters India 21. Central Institute for Cotton Research 22. Sathyabama University Osmania Central Research UniversityInstitute For Jute & Allied Fibres 25. Bishop Heber College, Trichi, TAMILNADU 26. Amity Institute of Biotechnology 27. Birla Institute of Technology Ministry University of of Environment Agricultural and Sciences, Forests - BANGALORE 30. Gauhati University 31. University of Agricultural Sciences Dharwad 32. Kerala university 33. Directorate of Rice Research 34. Junagadh Agricultural University Punjab Sher-e-Kashmir State Council University for Science of Agricultural & Technology Sciences 37. IARI Regional Station, Pusa, Bihar Central National Soil Research Salinity Centre Research for Rapeseed Institute Mustard 40. Central Institute of Freshwater Aquaculture 41. Apeejay institute of Management 42. Delhi University 1. Crop Design 2. Monsanto 3. Tranzpac 4. Emerging Solutions 5. JK-Agrigenetics Ltd 6. GrasslanD Technology 7. MAHYCO

27 Feedback Query Form

28 User`s Feedback

29 Objective 4 National Training Workshops on Biosafety Issues and Web Resources in GMOs Dr. R. K. Katiyar Course Director & Director National Research Centre on Plant Biotechnology, IARI, New Delhi rkkatiyar@hotmail.com Dr. K. R. Koundal Course Co-Director & Joint Director Research Indian Agricultural Research Institute, New Delhi jd_research@iari.res.in Dr. T. R. Sharma Course Coordinator National Research Centre on Plant Biotechnology, IARI, New Delhi

30 Numbers and Period of Trainings First Training : April 23-25, 2007 Second Training : May 14-16, 2007 Third Training : June 7-9, 2007

31 About the Participants and Resource Persons Participants Total No. of Participants = 45 ICAR Institutes Represented = 18 SAUs represented = 13 Resource persons ICAR DBT DU CSIR SAUs ICRISAT MOEF BCIL

32 Major Areas Covered in the training Biosafety issues Related to GMOs GM crops development and their post release management GMO detection in food feed and seeds Cartagena protocol & safe trans boundary movements of GMOs Environmental and health concerns related to GMOs Regulatory issues and regulatory agencies Availability and use of Web Resources on GMOs etc. Practical on Web based Resource Utilization

33 Training Manuals Developed

34 A Technical Bulletin

35 Some Events of the 1 st Training Workshop

36 Some Events of the 2 nd Training Workshop

37 Some Events of the 3 rd Training Workshop

38 Participants Feed Back about the Training

39 Outcome of the Project A Database: A web based Comprehensive database on Biosafety Issues related to GMOs Publications 1. Sharma, T.R. K Srivastava, S.Singh and K.R. Koundal Development of an Integrated Database on Biosafety and use of GMOS in India. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi Koundal K.R. and T.R. Sharma Biosafety concerns associated with GMOs. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi Sharma,T.R., K. Srivastava, S. Singh, A. Kumar, K. Gaikwad, K.C. Bansal, N.K. Singh and K.R. Koundal Web based Information Resources on GMOs. In CRTAGENA Potocols on Biosafety:Decisions to Diagnostics.(Eds Ramdhawa et l.2007)nbpgr,new Delhi 179p. Technical Bulletin Sharma TR GM Crops Database: An Interactive Web Resource. Pp. 16. Training Manuals 1.Sharma TR, KR Koundal, RK Katiyar st National Training Workshop on Biosafety Issue and Web Resources. April pp68 2.Sharma TR, KR Koundal, RK Katiyar nd National Training Workshop on Biosafety Issue and Web Resources. May pp76. 3.Sharma TR, KR Koundal, RK Katiyar rd National Training Workshop on Biosafety Issue and Web Resources. June 7-9. pp80

40 Infrastructure Developed under the Project SUN FIRE V240 SERVER SYSTEM : One number Storage Data Workstations : Two numbers. Developed Trained Human Resource in Bioinformatics Name of the personnel Period Present position Research Associates Ms Keerti Srivastava Mr. Ajay Kumar Mehato Senior Research Fellows Ms Sangeeta Singh Ms.Charu Singh Mr. N. Reddy to to to to to Joined in IT Company Tomato Genome Seq Project Pigeonpea Genome Project ICAR Network Project on Transgenic Crops Joined in Ph D

41 Proposed work for the 2 nd Phase Updating the Database. To develop a Network of Centres for Making Database in Regional Languages (Hindi, Punjabi, Tamil, Telgu and Kanada). Developing a simple version of the database for the farming community and common public.

42 Acknowledgements Prof. K.R. Koundal Joint Director Research, IARI Dr. PA Kumar Project Director, NRCPB Keerti Ajay Sangeeta Charu Financial Assistance Ministry of Env. & Forest, GOI GEF- World Bank

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