Rapid Microbial Detection Symposium Royal Society of Medicine 22 nd June 2011

Transcription

1 Rapid Microbial Detection Symposium Royal Society of Medicine 22 nd June by Smiths Smiths Detection: Detection: Proprietary Data

2 Smiths Detection today: tackling the widest range of threats What? Detecting/Identifying: Biological warfare agents Chemical warfare agents Toxic industrial chemicals Hazardous materials/gasses Explosives Radiation/nuclear materials Weapons Animal diseases Contraband Narcotics How? Techniques: Trace detection (IMS) Polymerase Chain Reaction X-ray imaging Millimetre-wave imaging Infrared spectroscopy Raman spectrometry Radiation spectroscopy Systems integration Sensor management (video over IP) Where? Transportation (principally airports) Ports & Borders Critical infrastructure Military Emergency response Diagnostics: Veterinary Clinical Making the world a safer place by supporting governments with the most advanced detection technologies to equip those who preserve global stability

3 Security and Inspection market Security & Inspection Wide range of technologies Checkpoint X-Ray Explosives Detection System Sensor management Cargo X-Ray Millimetre wave Searching for: explosives, weapons & contraband in a wide range of markets Explosives Trace Detection Airport Security Critical Infrastructure Ports & Borders

4 Biological Agents - Infectious Diseases- Diversity of the Target Wide range of sizes, shapes and levels of infectivity. Many related organisms which are harmless and abundant in the environment but cause cross reactivity. No False Alarms Bacteria Plague, Tularemia, Anthrax different strains, close similarity inactive versus active agent for testing antigen/antibody pairing Viruses Smallpox, Influenza, Ebola Toxins Ricin, Saxitoxin, Botulinum Toxin size,molecular variation, purity, e.g. Botox A - G plus subtypes

5 UK Vehicle Detection System Integrated Biological Detection System (IBDS)

6 Problems of Field Based Microbial Detection 1 Market not very clear what it wants! Generally done by non specialists, with other tasks to perform! Must have clear, simple to interpret result. Test must be rapid, 15 to 30 minutes ideally. Sample preparation required. Some sample reserved for gold standard testing & confirmation. High level of reproducibility & reliability required.

7 Problems of Field Based Microbial Detection 2 Low cost test Few or zero consumables preferred as 4 C storage can be problematic Difficult sample matrices, powder, soil, muddy puddle, aerosol collection fluid. Should not respond to interfering substances for example salt, coffee mate, paper dust, soil components Decontamination always an issue

8 Current Technologies Simple lateral flow type screen for Bacteria in general & specific toxin tests. Fluorescent readout and manual by eye readout for LFA. PCR for identification of bacteria and viruses. FTIR & RAMAN physical methods in parallel to determine chemical contents and possible interferant problems.

9 BIO-ACT TM Advanced Assessment & Confirmatory Technologies A combined approach allows efficient timely assessment of suspicious powders by utilising complementary technologies for measuring BWA HazMatID Ranger TM PrimeAlert TM BioCheck TM BioSeeq PLUS TM BIO ACT orthogonal approach

10 Future Directions Drive is smaller, more tests, easier to use. Improve lateral flow tests. For example easy to use, timing reader, nano particles for more signal. Microfluidic chip with variable screening tests and configurable architecture. Chip based, array determination for immuno methods, to give multiple tests per target and more confident result. Possible electrochemical style, or screen printed electrode for direct results.

11 Future Directions Drive is for low power, easily decontaminated kit, part of a network system for extra information. Sample preparation is a big issue. Needs to be automated and simple, but fast (15-30 mins) and inexpensive too. Identification technology is PCR preferred. Iso thermal and new methods not really established. Lab on a chip, array technologies, nanotubes and micro devices still some way off.

12 by Smiths Smiths Detection: Detection: Proprietary Data