Personal experiences on patenting part II
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- Shanna Gregory
- 5 years ago
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1 Personal experiences on patenting part II Marc Baumann Biomedicum Helsinki FIN University of Helsinki
2 Where did it all began? 1992
3 The preparative gel electrophoresis story! I was fed up with the existing technologies for protein purification and decided to
4 Make my own instruments for using electrophoresis in protein purification. For that I needed: 1) An idea 2) Money 3) Commercial education 4) A Company (or( many?)
5 The Idea!
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8 Money or more products? First collaboration with BioRad (BioRad is one of the largest biotechnology companies in the world!)
9 Mini-Prep Cell Let s go shortly to the BioRad www site
10 So my machine was finally combined with a commercial device Patents at this stage? No patents filed = and everything went very smoothly 3 years coll. with full support
11 But what about money? Where do you get money in Finland? >> everywhere and nowhere Whom to contact? University IPR office? Licentia? Helsinki Business and Science park? TEKES? Individual scientist? but whom? Telephone operator
12 I went to SPINNO SPINNO is an education from the UH and TKK which want to support spin-off from academia
13 My first contract
14 My first AWARD for a great invention (still not a patent)
15 Development of new products
16 Development of new products
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18 My first Company?
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21 So I had lived a nice life with alot of excitement Until one day a decided to go for PATENTS
22 My first patent Let s check the PRH site A drug for Alzheimers diseases! Now money comes in?
23 Filing a patent in the BIG Apple NYU (New York University) - no costs for the inventor -no hassle for the inventor -proper agreement with inventor -great opportunity
24 Designing Drugs Inhibiting protein misfolding in Alzheimer s Disease, Amyloidoses and Prion Disorders Marc Baumann Protein Chemistry/Proteomics Unit and the Neuroscience Program Biomedicum Helsinki marc.baumann@helsinki.fi
25 Protein misfolding, what can it do? Folded functional protein Conformational change Abnormal protein Non-folded Non-functional protein Can cause a loss of the physiological function Can cause aggregation and deposition Can become toxic
26 Examples of protein misfolding disorders Disease Alzheimer's disease, CJD, FFI, Kuru, BSE Protein involved Amyloid-ß protein Prion protein Parkinson disease α-synuclein, parkin (?) Huntington disease Diabetes type II Amyotrophic lateral sclerosis Serpin deficiency, emphysema, cirrhosis Haemodialysis amyloidosis, prostatic amyloid Cystic fibrosis CADASIL disease Huntingtin Amylin Superoxide dismutase Serpins ß2-microglobulin CFTR protein Notch3 receptor protein Etc, etc, etc...
27 Protein Aggregates in Conformational Disorders Alzheimer s amyloid plaques Prion plaques Parkinson s Lewy bodies X Aggregates Amyloid fibers
28 Sequences of Alzheimer s β-sheet breaker peptides N Aβ C APP Aβ1-42 DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGVVIA 1 42 β-sheet breaker peptides iaβ11 RDLPFFPVPID iaβ9 RDLPFFPVD iaβ7 LPFFPVD iaβ6 LPFFVD iaβ5 5 LPFFD iaβ4 LPFF iaβ3 PFF
29 My first patent Let s check the PRH site
30 My second patent Let s check the PRH site Micro-chips chips for proteomics
31 Filing a patent in Finland HY/TKK - alot of costs for the inventor -alot hassle for the inventor -no agreement with inventor -great opportunity = this must be a joke!!
32 The Idea!
33 Came out from GE-Healthcare
34 Protein Microchips for the Identification and quantification of Biomolecules
35 1-DE Chip electrodes gel reservoir: 25x32x0.3/0.5 mm PMMA, silica sample inlet buffer reservoir gel reservoir water cooling standard real sample Running time 10 minutes
36 1-DE Chip 10 mm A Buffer reservoir Micro Pipette Comb sample applicator sample loading o-ring gel buffer reservoir 30 mm 15 mm PASGE lid + - Gel plate Resolving gel Stacking gel 11 mm 25 mm B C D
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38 Proteomics performed by 2-dimensional Gel electrophoresis Basic (+) Acidic (-) #7 Protein Chemistry Unit Biomedicum Helsinki 2002
39 2-DE Chip The compress system
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41 2-DE Chip 2-D map of IEF standards 3-10 ph gradient Rf Repeatability of 2-DE runs Rf values (%) * pi position errors(%) * STDV 6,1 STDV 2,5 37 mm max min 15 0,8 max min 6 0,6 * comparison of 3 gels 25 mm 2-DE separation completed in approx. 80 min Limit of detection is approx. 65 ng Running time minutes
42 compress 2-DE 2 system Performance Native IEF and native PAGE 5 variants of hemoglobin ph Native IEF and SDS-PAGE standard IEF proteins ph 3-10 Denatured IEF and SDS-PAGE HbA 2 HbF HbS GFAP protein variants expression differences in control and Alzheimer diseased patients ph 4-6 HbA 1c HbA control AD
43 ComPres device/pressing of the gel Before After Gel was been press 0,05 mm to obtained filling of the channel between IEF and PASGE gel This is the only thing I have patented
44 Some details: We patented first in England = entrance to EU Then US, CAN, PTC-EU, AUS
45 All costs were covered by the inventors grants All together for the first round
46 There was not much help of: - Licentia - UH/TKK sp keep in mind that YOU have to do everything
47 What are your options? You have several options: 1) grab some money from the investors
48 and you are lost unless you find just one partner who is really interested in your idea and not in killing you
49 Let Licentia pay or get a loan
50 and you are lost unless you keep track that Licentia really does the selling job!
51 Get a partner who can provide you with support and understanding
52 and you are saved You might not get much money but you get support for several years
53 And you might get your invention to see the light! Is that not what we scientists mostly hope for?