Advanced. Anoxomat System. For the cultivation of anaerobic, microaerophilic, and capnophilic bacteria

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1 Advanced Anoxomat System For the cultivation of anaerobic, microaerophilic, and capnophilic bacteria

2 The Advanced Anoxomat TM is a system for creating anaerobic, microaerophilic, and capnophilic environments in jars. Anoxomat operates via an embedded computer to create exact, repeatable environmental conditions the resulting gas mixtures will always be within 0.5% of the required value. This high precision, combined with ensured reproducibility, allows microbiologists to draw accurate, confident conclusions. With the ability to create the required conditions quickly, the Anoxomat serves as an excellent alternative to gas bags and anaerobic chambers. Available multiple gas and jar connections for increased efficiency Easy-to-use touch screen operation Four different-sized jars to fit your laboratory s needs Data interface for downloading of data Optional printer for easy print out and archiving of test results and patient information A simplified version of the Anoxomat Mark II is available for laboratories that have modest needs. Baby Anoxomat offers only standard anaerobic and microaerophilic evacuation modes, but full QA functionality.

3 O2 level in the jar 15.00% 5.00% 0.00% Anaerobic recipe process time Minutes Level in jar Superior Recovery Due to the speed in which Anoxomat achieves the required conditions, the recovery of the micro-organisms cultivated in jars and processed by the Anoxomat system is superior to all other methods. 1 Anoxomat provides excellent recovery of clinical bacteria such as Helicobacter spp., Bacteroides spp., and other strict anaerobes; Clostridium spp. and Campylobacter spp. in food and even spoilage bacteria such as Megasphaera spp. and Pectinatus spp. in beer. 1 Summanen P.H., McTeague M., Väisänen M.-L., Strong C.A., and Finegold S.M. (1999) Comparison of recovery of anaerobic bacteria using the Anoxomat, Anaerobic Chamber, and GasPak jar systems. Anaerobe 5:5-9 The Anoxomat incorporates unique features that improve laboratory productivity Advanced ANOXOMAT Features and Benefits Rapid Complete anaerobiasis in less than 3 minutes for unmatched organism quality and recovery Cost efficient Low gas consumption, maintenance, and operating costs tremendously reduce costs over anaerobic chambers and gas bags Quality control Quality Assurance Program (QAP) performs leak tests and gas input test, plus confirms catalyst activity BEFORE incubation Multifunctional programming Create anaerobic, microaerophilic, and capnophilic, environments all with one instrument Custom recipes Utilize the optional Recipe Generating Program to mix any type of gas to create a custom environment Data record Optional User Traceability Program to document user ID, gas mixtures, and patient information Automatic, one-button operation Process jars while you continue to work and increase your laboratory s efficiency No disposables Anoxomat produces no chemical waste and eliminates the cost of disposal Superior environments Other methods are marred by disadvantages such as late discovery of faulty jars and gas packs, slow anaerobiasis, and chemical waste Compact design Massive space-occupying chambers take up valuable lab space. Anoxomat is no bigger than a small microwave oven Applications Clinical Cultivation of anerobes and microaerophiles from patient samples Food Creates a perfect environment for food spoilage and pathogenic bacteria Brewery Successful cultivation of beer spoilage bacteria for superior quality control Research The Anoxomat is a perfect tool for the study and cultivation of all anaerobic, microaerophilic, and capnophic bacteria The Evacuation-Replacement Method of MacIntosh and Fildes The Anoxomat s evacuation-replacement methodology rapidly removes an oxygenated environment from a jar and replaces it with a precise amount of an anaerobic gas mixture. After the anaerobic cycle, a mere 0.16% residual oxygen content is left in the jar, which is then removed by a small palladium catalyst. This method is the most efficient way to create anaerobic, microaerophilic, and capnophilic conditions % 22.00% 22.00% 1000 Mbar 18.00% 1000 Mbar 18.00% % % % % 14.00% 14.00% 14.00% Evacuation/ Replacement O2 level CO2: input 10% H2 level: input 5% Balance N2 input 85% 12.00% 8.00% 6.00% 4.00% 2.00% 0.00% Evacuation/ Replacement O2 level CO2: input 10% H2 level: input 5% Balance N2 input 85% 12.00% 8.00% 6.00% 4.00% 2.00% 0.00% % Evacuation/ Replacement O2 level CO2: input 100% 12.00% 8.00% 6.00% 4.00% 2.00% Anaerobic Cycle Microaerophilic Cycle Capnophilic Cycle

4 Advanced Anoxomat Mark II System ABOUT ADVANCED INSTRUMENTS Advanced Instruments, Inc., and our subsidiaries, Delta Instruments and Mart Microbiology, design and manufacture instrumentation for the clinical, pharmaceutical, biotechnology, microbiology, and food laboratories. Our quality brands include Spiral Biotech, Fiske Associates, and D & F Control Systems. The products we make help healthcare providers improve the quality of care and industrial companies enhance quality and productivity. Advanced Anoxomat Mark II System Specifications* Electrical Type: 220 V 110 V Voltage V V Frequency Hz Hz Fuses 1 & A slow 6.3 A slow Vacuum Pump Bush SV1003 Bush SV1003 Peak Current 1.1 Amp 4.3 Amp Housing Bottom: Top: Net Weight: Dimensions Width: Depth: Minimum height: Maximum height: Steel, powder coated ABS B300 IR, black, sandstone, mat; ABS B300 IR, white, smooth, mat 33.1 lb (Ca. 15 Kg) 12.1 in (32 cm) 21.1 in (55 cm) 3.1 in (8 cm) (front) 10.1 in (27.5 cm) (back) Operating temperature: F (10-32 C) Relative Humidity: % Ultimate pressure 150 mbar vacuum pump: *Specifications subject to change Advanced Anoxomat Mark II System Parts and Supplies Part # Description Anaerobic Jars AJ9022 Holds one stack of 6 Petri dishes, 9-10 cm dia. AJ9023 Holds one stack of 12 Petri dishes, 9-10 cm dia. AJ9025 Holds one stack of 12 Petri dishes, 9-10 cm dia. Holds one stack of microtiter plates, 13 x 9 cm dia. Holds one stack of 10 Petri dishes, 14.5 cm dia. AJ9028 Holds four stacks of 12 Petri dishes, 9 cm dia. Holds three stacks of 12 Petri dishes, 10 cm dia. Petri Dish Holders PH 1060 Holds 6 Petri dishes, 9-10 cm dia. PH 1040 Holds 12 Petri dishes, 9-10 cm dia. PH 1080 Holds three stacks of 12 Petri dishes, 6 cm dia. PH 1090 Holds one stack of microtiter plates, 13 x 9 cm dia. PH 1070 Holds 10 Petri dishes, 14.5 cm dia. PH 1050 Holds three stacks of 12 Petri dishes, 10 cm dia. Catalyst CA0000 For use with AJ9022, AJ9023, Quantity needed: 1 CA0000 For use with AJ9025, Quantity needed: 2 CA0000 For use with AJ9028, Quantity needed: 3 CA For use with AJ9025, AJ9028, Quantity needed: 1 1 For use only with AN2CTS The management system governing the manufacturing of this product is ISO 9001 and ISO registered. Advanced Instruments supplies The a full management line of jars, system catalysts, governing petri dish holders and other accessories to ensure optimal system performance. the manufacturing of this product is IVD ISO 9001 and ISO registered. Advanced Instruments products are available from a worldwide distributor network. For more information on our products and services or to find your nearest distributor, visit us at or us at info@aicompanies.com. Hot-Line Technical Service Advanced Instruments Hot-Line Service and worldwide distributor network provide comprehensive customer service and technical support Advanced Instruments. Advanced and Anoxomat are trademarks of Advanced Instruments, Inc. All other trademarks are the property of their respective companies. MKT ANOX 4/15/10

5 DATA SHEET Anoxomat Accessories and Supplies Avoid costly incubation failures with superior jar construction and quality control by utilizing the Anoxomat and the Advanced Anaerobic Jars. Strong cast acrylic material for durability Snap-shut coupling enabling quick connection to the Anoxomat system Four different jar sizes to accommodate a variety of plate sizes, microtiter plates, and test tubes Oxygen-sensitive organisms are routinely cultivated in anaerobic jars. Unfortunately, many brands on the market are poorly constructed and are not air tight. These defects regularly result in no-growth plates due to oxygen leaking into the jar. The Advanced Anoxomat jar satisfies the highest standards and will have a longer life than competing brands. Laboratory managers depend on their equipment for quality results. No other methodology lid clamp, the Advanced Anoxomat jar meets the Due to the quality of construction and the strong produces the quality manager s expect than the highest microbiological and mechanical standards. Anoxomat system. To be sure your anaerobic and A variety of autoclavable Petri dish holders are microaerophilic samples are processed properly, available for almost every size of dish, test tube, you must be confident your equipment is working and microtiter plate. Plus, each jar features a properly and providing optimum conditions. quick-connect coupling for easy connection to the Anoxomat in one swift move. The jar can be easily disconnected after processing.

6 Anoxomat Accessories and Supplies ABOUT ADVANCED INSTRUMENTS Advanced Instruments, Inc., and our subsidiaries Delta Instruments and Mart Microbiology, design and manufacture instrumentation for the clinical, pharmaceutical, biotechnology, microbiology, and food laboratories. Our quality brands include Spiral Biotech, Fiske, and D & F Control Systems. The products we make help healthcare providers improve the quality of care and industrial companies enhance quality and productivity. Palladium Catalyst After the anaerobic cycle is complete, a mere 0.16% oxygen concentration remains in the jar. A small catalyst is needed to facilitate the removal of the last trace amounts of oxygen to achieve complete anaerobiasis. The catalyst is palladiumcoated aluminum pellets in a stainless steel sachet. The catalyst can be used numerous times by reactivation in a hot-air oven. Halamid Jar Cleaner and Disinfectant Thermal Printer for Sample Traceability The Anoxomat software can be upgraded with the sample traceability program. With this feature, data can be downloaded into a database or printed on thermal paper. The printout can be clipped to the jar for identification before and after incubation. Halamid cleaner is a chlorine-based and alcoholfree disinfectant safe to use in food and healthcare settings. Halamid should only be used with Advanced Anaerobic jars to ensure no residual alcohol is left behind and that the cleaner being used doesn t degrade the jar or lid. Effective against bacteria, fungi, and viruses Releases exactly the right amount of chlorine needed: Halamid retains its active properties even after long periods of time Excellent storage stability Not aggressive against metals, rubber, and plastic Easy to rinse and dissolves easily in cold water Advanced Instruments products are available from a worldwide distributor network. For more information on our products and services or to find your nearest distributor, visit us at or us at info@aicompanies.com. Hot-Line Technical Service Advanced Instruments Hot-Line Service and worldwide distributor network provide comprehensive customer service and technical support Advanced Instruments. Advanced and Anoxomat are trademarks of Advanced Instruments, Inc. All other trademarks are the property of their respective companies. MKT ANOX-ACC 4/15/10