MP Biomedicals ISOLATE CULTURE DETECT. Provide you the Best Immunology Solutions. Click, Pick and Publish.

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1 2013/2014 One Call One Source MP Biomedicals Provide you the Best Immunology Solutions A World of Immunology ISOLATE CULTURE DETECT Click, Pick and Publish.

2 A Message From Our Chairman Milan Panic Chairman and CEO MP Biomedicals, LLC Everybody has priorities. You are mine. Dear valued customer, I am pleased to present our newest MP Biomedicals Immuno Solutions brochure. If you are already familiar with our world-class company, then you know our superb service, unequaled quality, and track record of fast delivery. If this is your fist visit to then I welcome you to peruse the many thousands of top quality products contained herein.mp Biomedicals is a world-leading supplier of Life Science, Molecular Biology, Immunochemical and Biochemical products to meet your needs. As you perhaps know, I started this company in 1958 and, although this company originally operated under a different name, our commitment to superior products has never changed. While MP Biomedicals offers over 50,000 products in research-size packages, they are also available in bulk and semi-bulk for use in the manufacture of intermediates in large scale production at competitive prices through our Fine Chemicals Division. It is my top priority to focus MP Biomedicals resources on providing superior service, top quality, and fast delivery. For your convenience, our main catalog and website contain information about the products you seek for all of your projects and needs. As always, I invite your comments and suggestions regarding our catalog, website, products, and services. Please feel free to me at sales@mpbio.com. Sincerely, Milan Panic Chairman and CEO 2 Please Please order order online online at at or or call call

3 ISOLATE Why MP Biomedicals? 1. Quality - We supply top quality tools to help in your research 2. Service - You are our priority 3. Price - Competitive price giving you the best value for the quality Choose one of three formulations. Visit us at to request free samples and take advantage of special web promotions X 100 ml Lymphocyte Separation Medium - LSM x 100 ml ml LymphoSep - Lymphocyte Separation Medium ml Mono-Poly - Mono Poly Resolving Medium 100 ml LSM from MP Biomedicals has been cited in 150 publications in the past 3 years. The testimonial speaks for the quality and gives you the peace of mind in cell separation! Expression Profiling of Human Immune Cell Subsets Identifies mirna/mrna using LSM from MP Biomedicals Figure 1. Cell type specific expression of mirnas. mirnas were grouped based on specificity to one, two or three cell types. Figure mrna transcripts are uniquely up or down-regulated. Florence Allantaz et. Al., (2012), Regulatory Relationships Correlated with Cell Type Specific Expression. Hoffmann-La Roche Ltd. PLoS ONE 7(1): e doi: /journal.pone Please order online at or call

4 LSM - Lymphocyte Separation Media MP Biomedicals LSM is a proprietary formulation which allows one-step separation of lymphocytes from peripheral blood. LSM from MP BIomedicals offers you the best results in mammalian cell separation. Your piece of mind in cell separation is guaranteed with: Maximum yield of lymphocytes Easy one-step centrifugation Ready-to-use, sterile filtered formulation Guaranteed >96% of isolated lymphocytes will be viable LSM is a sterile-filtered solution of a sucrose polymer and diatrizoate salts at a specific gravity of g/ml at 20 C. LSM is formulated for isolation of mononuclear cells from defribinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets. LymphoSep is a sterile, isosmotic, low viscosity Ficoll -Sodium Metrizoate medium which offers a quick and reliable method for the simple isolation of lymphocytes from whole blood. It is certified for In Vitro Diagnostic (IVD) use and facilitates the isolation of lymphocytes from defribinated, EDTA or heparin treated human blood. Mono-Poly Resolving Medium is a solution composed of a polysaccharide (Ficoll 400) and a radiopaque contrast medium (Hypaque). The resolving medium can be used for a variety of research and clinical applications including histocompatability testing, lymphokine studies, in vitro cell mediated immunity assays, and any procedures requiring a pure WBC preparation. Separation of mononuclear and polymorphonuclear leukocytes into two distinct bands. > 95% cell viability Rapid, easy and reliable one-step procedure No sample dilution requirement or lysis of red blood cells 4 Please Please order order online online at at or or call call

5 CULTURE MP Biomedicals offers the highest quality and a broad range in mammalian cell culture media, serum free media, supplements and antibiotics. Our products have been highly validated and cited in scientific publications which provides you the confidence in your research results. Best in quality Validation based on refereed publications Unique and wide range portfolio Convenient packaging for various needs Custom formulations made to your specifications Mammalian Cell Culture Media and Salt Solutions Classical media in powdered and liquid formats for mammalian cell culture applications The cell culture media products are designed to support optimal cell growth under defined conditions. The products offer the best performance for your cells and are guaranteed to be of the highest quality and consistency. Our media guarantees your satisfaction: All products undergo rigorous quality control testing and are tested for endotoxin and toxicity. Salt solutions are produced without sodium bicarbonate to increase stability. Organization of MCF7 Cells in Cell Culture Media from MP Biomedicals Silva Krause, et.al., 2010, 10:263. The microenvironment determines the breast cancer cells phenotype: organization of MCF7 cells in 3D culture. BMC Cancer, 10:263. Epithelial structures observed in collagen gels. Panel A depicts representative H&E and whole mounts (WM) of MCF7 cells grown alone in collagen gels for 1-6 weeks. Dead cells are depicted by black arrows. Panel B shows MCF7 cells grown in co-culture with RMF in collagen gels. Note the survival effect provided by the presence of RMF already at week 1. Arrowheads depict fibroblasts, blue arrows depict polarized structures, and *illustrates apoptotic cells. Scale bars: H&E = 50 μm, WM = 100 μm. Please order online at or call

6 RPMI, Liquid RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes and is now a popular general purpose Sodium Sodium Phenol Cat. No. Dextrose L-Glutamine Other Present (+), Absent (-) Size Price Price Pyruvate Bicarbonate Red ml ml (+) 20 mm HEPES 500 ml (-) Phosphate 500 ml (-) Leucine 100 ml ml (-) Methionine, (-) Cystine, (-) 500 ml ml RPMI, Powder x 1 L x 10 L x 50 L DMEM, 1X Liquid Dulbecco s Modified Eagle s medium contains four times greater concentration of amino acids, vitamins and supplementary components than Eagle s Basal medium ml ml (-) Leucine 100 ml (-) Methionine 500 ml (-) Phosphate 500 ml (-) Methionine, (-) Cystine, (-) 100 ml (-) Inositol 500 ml ml DMEM, Powder x 1 L x 10 L F-10 Nutrient Medium, Liquid Ham s F-10 and F-12 media are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham s F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells (+) 20 mm HEPES 100 ml ml F-10 Nutrient Medium (Ham s F-10), Powder x 10 L F-12 Nutrient Medium, Liquid ml F-12 Nutrient Medium, Powder x 10 L DMEM/F12 Nutrient Medium, Powder x 10 L x 10 L Joklik s Modification of Minimum Essential Medium (JMEM), Powder (+) Penicillin, (+) Streptomycin 1 x 10 L Please Please order order online online at at or or call call

7 MEM, Liquid Modified Eagle s Medium incorporates specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP s MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. L-Glutamine Pyruvate Bicarbonate Sodium Sodium Phenol Cat. No. Dextrose Other Present (+), Absent (-) Size Price Price Red ml ml X6 6x500 ml (+) Earle s Salts (-) Arginine 100 ml (+) Earle s Salts (-) Methionine, (-) Cystine, (-) Cysteine 500 ml (+) Hank s Salts, 20 mm HEPES 500 ml (+) Hank s Salts 500 ml MEM 10X (+) Earle s Salts 500 ml MEM 50X 100 ml MEM, Powder (+) Earle s Salts 10x1 L (-) Deoxyribosides (-) Ribosides 10x1 L BME Liquid BME 100X (+) Vitamins 100 ml Primary Culture & Leucocytes Media 1X McCoy 5A Medium (Park and Terasaki Modification) without L-glutamine This medium supports the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP s medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types Medium 199 with Earl s Salts (E199) Medium 199 can be used with a broad range of species including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle s or Hank s salts. Primary Culture & Leucocytes Media McCoy's 5A Medium (Iwakata and Grace Modification) (1X Solution) with L-glutamine 500 ml McCoy's 5A Medium (Park & Terasaki Modification) (1X Solution) without L-glutamine, sodium bicarbonate 100 ml Medium 199 with Earle's Salt (E199), with 20 mm HEPES, without L-glutamine and sodium bicarbonate 100 ml X Medium 199 with Earl's salts (E199), with L glutamine, without sodium bicarbonate 10 x 1 L X Medium (H199 Powder) with Hank's salts with L-glutamine without sodium bicarbonate" 10 x 1 L Please order online at or call

8 Serum Free Media and Supplements The use of fetal bovine serum in cell culture media can lead to concerns arising from biological contamination, higher complexity in assays and downstream purification. Additionally, lot-to-lot variation in performance impacts sourcing and testing. MP Biomedicals wide range of Serum Free products puts your mind at ease when it comes to cell culture. Our serum free products are: Well defined with minimal variability Better control and consistent performance Ideal for evaluation of cellular functions in assays Hematopoietic Stem Cell Line, HPC-7 Grown in Serum Free Media Kavanagh DPJ, et al. (2013) Mechanisms of Adhesion and Subsequent Actions of a Haematopoietic Stem Cell Line, HPC-7, in the Injured Murine Intestinal Microcirculation In Vivo. PLoS ONE 8(3): e doi: /journal.pone pone Preserve your serum free cell line - Cellvation Cellvation is a cryopreservation medium which contains neither DMSO nor Serum and is ideally suited for serum-free cultures. Cellvation offers; Optimal recovery and faster cell attachment Suitability for all types of serum-dependent cell lines A solution which is ready to use Serum Free Media CELLect Serum-Free Medium, Mammalian 500 ml BioRich 1 - DMEM/F-12 powdered media blend enriched with trace elements 1x10 L Cellvation Cryopreservation Serum Free Medium 20 ml M2 Cellvation Cryopreservation Serum Free Medium 60 ml Ham's F-10 Nutrient Mixture - Liquid, (+) 20 mm HEPES 100 ml Ham's F-10 Mixture - Liquid 100 ml Ham's F-10 Nutrient Mixture - Powder 1 x 10 L Ham's F-12 Nutrient Mixture - Liquid 500 ml Ham's F-12 Nutrient Mixture - Powder 1 x 10 L Please Please order order online online at at or or call call

9 MP-Hybridoma Solutions Focused on hybridoma research? Look no further than MP Biomedicals for all of your needs pertaining to serum free media and supplements. Our Serum free medium offers you distinct advantages: MP-Hybridoma Medium, Serum Free (NEW) Low protein content (11ug/mL) Chemically defined, no animal derived products Supports hybridoma cells, as well as lymphoid and epithelial cells Opti Clone II Hybridoma Cloning Factor Opti-Clone II is a serum-free growth medium supplement which improves the cloning efficiency of murine B cell hybridomas. Highly efficient growth supplement - up to 25% higher yields of mabs Increases cell health higher viability of cells Protein free - facilitates downstream purification Chemically defined no variation in performance means consistent results Colonies % RPMI % FBS Hybridoma Growth after fusion 73 7% RPMI % FBS + Growth Supplement % 100% 80% 60% 40% 20% RPMI % FBS + Opti-Clone II number of colonies % colonies producing antibodies Fig. 1: Opti-Clone II increases the number of antibody producing colonies IgG Production [mg/ L Hybridoma Cell Culture Fig. 2: Production of IgG using a proprietary serumfree adapted hybridoma cell line cultured in RPMI and insulin with and without Opti-Clone II [hours] RPMI + insulin RPMI + insulin + 10% Opti-Clone II Opti Clone II Hybridoma Cloning Factor Opti-Clone II - Hybridoma Cloning Factor 10 ml Opti-Clone II - Hybridoma Cloning Factor 50 ml Hybridoma Cell Growth Supplement 25 ml MP-Hybridoma Medium, Serum Free w/o Glutamine 500 ml MP-Hybridoma Plus Medium, Serum Free w/o Glutamine/Phenol Red 500 ml Please Please order order online online at at or call or 00 call

10 ChromatoPur Proteins (NEW) ChromatoPur proteins have been purified by chromatography and ensure the highest purity, consistency and activity in cell culture. Unlike with other extraction procedures (e.g. Cohn, Heat Shock) ChromatoPur proteins are more biologically intact and highly retain their cell growth stimulating activity. Proteins are purified from New Zealand sourced material using cgmp in an ISO 9001 certified facility which is audited by NZFSA MAF. ChromatoPur proteins offers: Effect of ChromatoPur TM on cell growth. Superior performance over Cohn s Albumin Fraction V Intact proteins with high activity Highest purity and consistency Chromatopur Products ChromatoPur Bovine Albumin, Low IgG, ImmunoAssay Grade 100 g ChromatoPur Bovine Albumin, Low Free Fatty Acid, ImmunoAssay Grade 25 g ChromatoPur Bovine Albumin, Protease Free, ImmunoAssay Grade 25 g Media Supplements Supplemental nutrients and reagents are essential for many cell cultures. Additives such as antibiotics and buffers are frequently used to prevent bacterial contamination, control ph and monitor culture conditions. Amino acids and vitamins are useful for enriched media. MP Biomedicals offers a wide choice of media supplements to meet your cell culture needs (see our web site for complete listing of media supplements). Vitamins, Amino Acids and Trace Solution Basal Medium Eagle Vitamin Concentrate (100X Solution) 500 ml Minimum Essential Medium Eagle Non-Essential Amino Acids 100 ml Minimum Essential Medium Eagle Non-Essential Amino Acids (50X) 100 ml Trace Element Solution 1, (1000X Solution) Sterile Filtered 100 ml Please order online at or call

11 Antibiotic Solutions Antibiotic solutions from MP Biomedicals are proven effective at keeping mammalian cell cultures free of bacterial or fungal contaminations. Antibiotic solutions are also used for clonal selection. Antibiotics Solutions Penicillin-Streptomycin Solution (50X Pen-Strep): Penicillin- 5,000 IU/mL, Streptomycin- 5 mg/ml Penicillin-Streptomycin Solution (100X Pen-Strep): Penicillin- 10,000 IU/mL, Streptomycin- 10 mg/ml Penicillin-Streptomycin-Amphotericin B Solution (100X Pen-Strep-Amphotericin B): Penicillin- 10,000 IU/mL, Streptomycin- 10 mg/ml, Amphotericin B- 25 μg/ml 100 ml ml ml Amphotericin B 250 mg/ml 20 ml Gentamycin Solution 10 mg/ml 10 ml Kanamycin Solution 5 mg/ml 50 ml Visit us at to request free samples and take advantage of the special web promotions. Please Please order order online online at at or call or call

12 DETECT MP Immunodetection reagents encompass 10,000+ mono, polyclonal and secondary antibodies, purified antigens, detection reagents and supplementary reagents. These reagents are the best in quality, highly validated, cited in refereed journals and offer the best value. Try our best sellers! Primary Antibodies Antibodies for Detection of GFP best in quality, reliable and most recognizable! Clone 3E6 is an IgG2a monoclonal antibody that recognizes the native forms of Green Fluorescent Protein (GFP), GFP variants and GFP-fusion proteins. Clone 3E6 is highly specific and sensitive for applications in mammalian and bacterial cells; Immunoprecipitation: 1-5 mg of antibody/sample Immunocytochemistry: 1:100-1:500 dilution ELISA: 1:1000-1:5000 dilution Coating antibody: 2-10 mg/ml Purification of GFP-fusion using GFP antibody coupled to sepharose beads Determine optimal dilution based on your application Embryo stained with anti-gfp monoclonal antibody clone 3E6 from MP Biomedicals Hatzold J, Conradt B. Control of apoptosis by asymmetric cell division. PLoS Biol 6(4): e84. doi: /journal.pbio epub 2009 Nov 12. Lee, KJ, et.al., Beta amyloid-independent role of amyloid precursor protein in generation and maintenance of dendritic spines. Neuroscience Aug 11;169(1): doi: /j.neuroscience Epub 2010 May 6. Sato, A, et.al., Cytoskeletal forces span the nuclear envelope to coordinate meiotic chromosome pairing and synapsis. Cell 2009 Nov 25;139(5): doi: /j.cell Epub 2009 Nov Please Please order order online online at at or or call call

13 Detect your GFP fusion protein in Western Blots! Clone 11E5 is a mouse IgG1 monoclonal antibody that recognizes the denatured form of the GFP, GFP variants and GFP fusion proteins. Detect as little as 10ng of GFP using colorimetric methods or pictogram quantities with chemiluminescent techniques. Western blot analysis and immunoprecipitation of the GFP-tagged proteins using MP Biomedicals antibody against GFP. strain: N2 N2 GFP::ALG-1/2 GFP::ALG-1/2 let-7 unbound IP with a-gfp 2 -O-Me aliga: Input let-7 Un GFP-ALG-1/2 bound Hutvágner G, et.al., (2004) Sequence-Specific Inhibition of Small RNA Function. PLoS Biol 2(4): e98. doi: / journal.pbio Anti-GFP Antibodies Anti-GFP - Clone 3E6 100 mg Anti-GFP - Clone 3E6 1 mg 1, , Anti-GFP - Clone 11E5 100 mg Please Please order order online online at at or call or 00 call

14 Monoclonal Antibodies to Detect Actin and Tubulin Antibodies to cytoskeletal proteins are widely used for protein loading controls or specific studies such as apoptosis. MP Antibodies to actin (clone c4) have demonstrated specificity directed towards all six known vertebrate isoactins. Our anti-tubulin monoclonal antibodies enable researchers to visualize mictotubules in fixed cells and in fixed or frozen tissue sections from various species. Detects all know actins or tubulins _Excellent positive control for Western Blots, Immunofluorescence and Immunohistochemistry No known spurious activities Actin intracellular visualisation using MP Biomedicals antibody against Actin Bär S et.al., 2008: Vesicular Egress of Non-Enveloped Lytic Parvoviruses Depends on Gelsolin Functioning. PLoS Pathog 4(8): e doi: /journal.ppat Anti-Actin and Tubulin Anti-Actin - Clone C4 Monoclonal Antibody 100 ml Anti-Actin - Clone C4 Monoclonal Antibody 200 ml A Anti-Tubulin (K-alpha-1) Monoclonal Antibody 100 mg α-tubulin Monoclonal Antibody 1 ml β-tubulin Monoclonal Antibody 0.5 ml Please order online at or call

15 Primary Antibodies - Epitope Tag Antibodies Epitope Tags are small peptides easily identified by specific antibodies. The fusion of these few amino acids to the targeted protein facilitates purification and detection procedures. MP Biomedicals offers three epitope tagged, high quality antibodies with outstanding specificity and efficient performance in various applications: Western Blot (WB) Fluorescence Microscopy (FM) Immunohistochemistry (IHC) Immunoprecipitation (IP) Flow Cytometry (FACS) Protein purification Anti-c-Myc Anti-c-Myc is able to detect the fusion protein of the Myc epitope cloned either to the amino- or carboxy- termini of targeted proteins. This antibody is suitable for ELISA and Western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Anti-c-Myc Rabit Anti-c-Myc 100 mg c-myc Monoclonal Antibody 100 mg Chicken c-myc Epitope Tag 100 mg Rabbit c-myc Epitope Tag 100 mg Anti-Hexahistidine (6xHIS) Antibodies to 6xHIS epitope tags H-H-H-H-H-H (also called anti-his, anti-hexahistidine, anti-polyhistidine, etc) can recognize antigenic determinants (epitopes) on recombinant proteins. The antibody recognizes the His-tag fused to amino- or carboxy-termini of targeted proteins in transfected or transformed cells. Anti-Hexahistidine (6xHIS) Rabit Anti-Hexahistidine (6xHIS) 100 mg Chicken Anti-Hexahistidine (6xHIS) 100 mg Anti-DYKDDDDK (FLAG) The FLAG epitope tag DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) is a small but highly immunogenic peptide, which allows fusion proteins to retain their original conformation and function. The hydrophilic character of FLAG peptide increases the likelihood that it will be located on the surface of the fusion protein where it is accessible to antibodies. Anti-DYKDDDDK (FLAG) Rabit Anti-DYKDDDDK 100 mg FITC Conjugate Rabbit Anti-DYKDDDDK 100 mg Please order online at or call

16 High Throughput Monoclonal Antibodies MP Biomedicals offers you monoclonal antibodies to cellular targets which are internal and external. The antibodies are highly purified, application tested and guaranteed for quality. Our monoclonal antibodies offer you the best performance for value: Specificity Immunogens based on gene sequence Immunogens expressed using recombinant DNA technology Sensitivity Combination of low dose immunization techniques and use of a proprietary adjuvant Purity Antibodies are purified using Protein A Chromatography Immunogens mimic the native proteins Use an in vitro wheat germ system to express the immunogens Expressed peptide mirrors the confirmation and folding of the native protein Validity Antibodies are tested in appropriate cell lines and tissues Each antibody is extensively application tested Dilutions recommended for each application Search for the Monoclonal Antibody of your choice for one of the following areas: Epigenetics Nucleic Acid Binding Transcription Apoptosis Ubiquitin/Proteosome Signal Transduction Enzyme Kinases/Phosphatases Metabolism Cell Adhesion Cell Membrane Cell Cycle 16 Please Please order order online online at at or or call call

17 Secondary Antibodies First Class Quality and Service MP Cappel secondary antibodies offers the highest specificity, quality, titer and the widest selection against human, mouse and rabbit immunoglobulins. Multiple forms are available: unconjugated, enzyme or fluoro conjugated. Enzyme (Alkaline phosphatase (AP) or Horseradish peroxidase (HRP) conjugated antibodies are suitable for EIA, ELISA, Blot Immunostaining and cell/tissue staining. Fluorochrome conjugated antibodies are used for immunofluorescence assays, cell/tissue staining, and fluorescence-activated cell sorting (FACS). Immunofluorescence Antibodies to Human Immunoglobins Antibodies to Human IgA Cat. No. Conjugate Host Fraction Size Price Price FITC Goat IgG 2 ml Antibodies to Human IgG (whole molecule) None Goat F(ab )2 2 ml None Goat Affinity Purified 2 mg FITC Goat F(ab )2 2 ml HRP Goat IgG 2 ml HRP Goat Affinity Purified 2 ml Alk Phos Goat Affinity Purified 1 ml Human IgG Fc None Goat F(ab )2 2 ml None Goat Affinity Purified 1 mg None Goat Affinity Pure 2 mg HRP Goat F(ab )2 2 ml Human IgG F(ab ) None Goat Affinity Pure 2 mg FITC Goat IgG 2 ml HRP Goat IgG 2 ml Human IgM None Goat IgG 5 ml None Goat Affinity Pure 1 mg FITC Goat IgG 2 ml FITC Goat Affinity Purified 2 mg Immunohistochemistry Anti-human IgG1 Fc antibody used as a secondary antibody in Western Blotting. Hu Q-Y,et al. (2010) Fine Definition of the CXCR4-Binding Region on the V3 Loop of Feline Immunodeficiency Virus Surface Glycoprotein. PLoS ONE 5(5): e doi: /journal.pone Human IgG, IgA, IgM None Goat Affinity Purified 2 mg FITC Goat IgG 2 ml FITC Goat F(ab )2 2 ml Alk Phos Goat Affinity Purified 1 ml Human Kappa Light Chains FITC Goat IgG 2 ml FITC Goat F(ab )2 2 ml HRP Goat IgG 2 ml FITC Goat IgG 2 ml Please Please order order online online at at or call or 00 call

18 Antibodies to Rabbit Immunoglobulins Antibodies to Rabbit IgG Fc Cat. No. Conjugate Host Fraction Size Price Price None Goat Affinity Purified 2 mg FITC Goat F(ab )2 10 mg Antibodies to Rabbit IgG F(ab ) None Goat Affinity Pure F(ab )2 2 mg HRP Goat Affinity Purified 2 ml Antibodies to Rabbit IgG (Whole Molecule) None Goat IgG 5 ml FITC Goat IgG 2 ml HRP Sheep IgG 2 ml HRP Goat Affinity Purified 2 ml ALK Phos Goat Affinity Purified 1 ml Antibodies to Mouse Immunoglobulins Antibodies to Mouse IgG (Whole Molecule) None Goat Antiserum 2 ml None Rabbit Antiserum 2 ml None Goat Affinity Purified 2 mg None Goat Affinity Purified 2 mg None Goat Affinity Purified F(ab )2 2 mg FITC Goat IgG 2 ml FITC Sheep IgG 2 ml FITC Goat IgG 2 ml HRP Goat IgG 2 ml HRP Goat Affinity Purified 2 ml Alk Phos Sheep Affinity Purified 1 ml Biotin Goat Affinity Purified 2 ml Antibodies to Mouse IgG Fc None Goat Affinity Purified 2 mg HRP Goat Affinity Purified 2 ml Alk Phos Goat Affinity Purified 1 ml Antibodies to Mouse IgG, IgA, IgM FITC Goat IgG 2 ml FITC Goat Affinity Pure F(ab )2 2 mg Rhod Goat Affinity Purified 2 mg HRP Goat IgG 2 ml HRP Goat Affinity Purified 2 ml HRP Goat Affinity Pure F(ab )2 2 ml To find more information on Antibodies, visit 18 Please Please order order online online at at or or call call

19 Detection Reagents Anti-Enzyme Alkaline Phosphatse (AP) and Horseadish Peroxidase (HRP) are two major enzymes that are used for secondary detection. MP Bio has a broad range of anti-enzyme products available in various conjugation forms from many different species. Anti-Enzyme Goat IgG Fraction to Alkaline Phosphatase 2 ml Goat Antiserum to Horseradish Peroxidase 2 ml Rabbit Antiserum to Horseradish Peroxidase 2 ml Goat IgG Fraction to Horseradish Peroxidase 5 ml FITC-conjugated Goat IgG Fraction to Horseradish Peroxidase 2 ml Goat Peroxidase Anti-Peroxidase 1 ml Rabbit Peroxidase Anti-Peroxidase 5 mg Avidin-Biotin Streptavidin, avidin and biotin conjugated with AP, HRP or FITC are ideal to amplify signals in immunochemistry, ELISA, flow cytometry and Western botting. Our AP Avidin, Avidin-HRP and Streptavidin-FITC for use as secondary reagents allow further enhancement of signal. Avidin-Biotin Alkaline Phosphatase-Avidin 2 ml Avidin-HRP 5 mg Streptavidin-FITC 1 mg Protein A and G Recombinant protein A (from Streptococcus sp) binds immunoglobulins (primarily IgG) at the Fc region with high affinity. This unique property provides a rapid, simple and economical method for purification and analysis of a broad-spectrum of antibodies. AP Biotin or colloidal gold conjugated Protein A are versatile reagents which can be used for many immunological assays. Recombinant protein G is conjugated with aminohexanoyl biotin N-hydroxysuccinimide under optimum conditions and is free of unconjugated biotin. This conjugate reacts with the Fc portions of immunoglobulins of various species. Protein A and G Protein A 5 mg Protein A-Alkaline Phosphatase 1 mg Protein A-Biotin 500 mg Protein A-Colloidal Gold (Particle Size: 10 nm) 1 ml 1, , Protein A-Colloidal Gold (Particle Size: 20 nm) 250 ml Protein A-HRP 2 mg Protein G-Biotin 1 mg Please Please order order online online at at or call or call

20 Immunohistochemistry IHC-Revealer Reveal immunoreactive sites altered by fixation Obtain superior staining over commonly used enzyme pretreatments IHC-Revealer IHC-Revealer 10 ml DAB Tablets Detect HRP in membrane-bound and in situ applications Ideal for immunohistochemistry DAB Tablets DAB Tablets (3,3'-Diaminobenzidine) 100 tablets Coombs Reagents Diagnose autoimmune haemolytic anaemia (AIHA) using Anti-globulin (Coombs) test. Coombs Reagents Coombs' Test Canine 1 ml Coombs' Test Feline 2 ml Liver S9 Homogenate Rat Liver S9 Homogenate is tested for biological activity by conducting mutagenesis titration curves with at least one of the following three tests: benzo(a) pryrene, 2-anthramine, and 2-acetylaminofluorene. Liver S9 Homogenate Liver S9 Homogenate, Rat, in KCl 2 ml Liver S9 Homogenate, Rat, in Sucrose 2 ml Protease Protease S. aureus V8 is an excellent tool for proteolytic digestion. The enzyme specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids. In the presence of ammonium, the specificity is limited to glutamic sites. Protease Protease S.aureus V8 5 mg Please order online at or call

21 Purified Antigens Ideal for labeling, immunization, optimized antibody production and other applications. Protein A and G Tubulin Purified 1 mg HDL Purified Human 10 mg IgG, Human, 95%, Chromatographically Purified 50 mg IgA, Human, 95%, Chromatographically Purified 5 mg IgG, Mouse, 95%, Chromatographically Purified 20 mg IgG, Rabbit, 95%, Chromatographically Purified 50 mg IgG, Rat, 95%, Chromatographically Purified 20 mg Albumins Widest range of albumins from many species for various applications: Blocking agents to prevent non-specific adsorption Stabilizers/diluents for RIA or EIA and other immunoassay techniques Bovine Albumin, Liquid Solutions Albumin, Bovine, 35%, Sterile 50 ml Albumin, Path-4, 35%, Immunohematology 50 ml Albumins for other Species Albumin, Human, Low Endotoxin, Cohn Fraction V 1 g Albumin, Human, Chromatographically Purified 50 mg Albumin, Human, Heat Shock 10 g Albumin, Human, RIA Grade 10 g Albumin, Human, 30% solution 50 ml Albumin, Human, Protease Free, 10% 50 ml Albumin, Dog, Heat Shock 50 mg Albumin, Hamster 25 mg Albumin, Mouse, Chromatographically Purified 50 mg Albumin, Rabbit, Chromatographically Purified 50 mg Albumin, Rat, Heat Shock 100 mg Albumin, Rat, Chromatographically Purified 25 mg Please Please order order online online at at or call or 00 call

22 Contamination Control Mycoplasma Removal Agent (MRA) Eliminate mycoplasma contamination without harming your cell lines! Elimate mycoplasma completely from contaminated cultures by inhibiting mycoplasma DNA gyrase Easy to use Obtain clean cultures within a week Kills most common mycoplasma strains Prevents mycoplasma contamination with low cytotoxicity Prevents re-contamination with MRA at low doses Mycoplasma infected cell culture MRA MYCOPLASMA REMOVAL AGENT 5 ml MRA treated cell culture Stem Cell Antibodies High Specificity, Sensitivity and Reliability Stem cell research is a complex and exciting field that promises fantastic discoveries in numerous areas from cancer to diabetes to neurodegenerative diseases. MP Biomedicals offers a range of stem cell antibodies to advance work in this area. To learn more about our offering visit us at Visit us NOW at ufind products uexplore new products & applications uplace orders online uview order history & status 22 Please Please order order online online at at or or call call

23 Stem Cell Antibodies - High Specificity, Sensitivity and Reliability Visit to view the entire selection of stem cell antibodies and take advantage of our special pricing! Stem cell research is a complex and exciting field that promises fantastic discoveries in numerous areas from cancer to diabetes to neurodegenerative diseases. MP Biomedicals offers a range of stem cell antibodies to advance work in this area. Cat. No. 08A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A Description ACTG1 monoclonal antibody (M01), clone 1G6 AKAP7 monoclonal antibody (M13), clone 1F9 BM039 monoclonal antibody (M01), clone 4A5-1C11 BTF3 monoclonal antibody (M01), clone 3C4-2E11 C15orf15 monoclonal antibody (M01), clone 2A10-1E5 C1QBP monoclonal antibody (M01), clone 1F3 CCT5 monoclonal antibody (M01), clone 4E5-4B1 CD24 monoclonal antibody (M04), clone 1C4 CER1 monoclonal antibody (M04), clone 3E11 CKAP2 monoclonal antibody (M04), clone 3H1 CYB5A monoclonal antibody (M05), clone 4C2 DCPS monoclonal antibody (M02), clone 1G4 DDT monoclonal antibody (M01), clone 1G1 DNPEP monoclonal antibody (M01), clone 2F9-3A7 ETV1 monoclonal antibody (M01), clone 2A8 FLJ20643 monoclonal antibody (M04), clone 1G6 FOXA1 monoclonal antibody (M01), clone 2D7 FSD1 monoclonal antibody (M01), clone 1G3 GAPDH monoclonal antibody (M03), clone 1G5 GART monoclonal antibody (M01), clone 4D6-1D5 GEMIN6 monoclonal antibody (M08), clone 3D2 GREM2 monoclonal antibody (M02), clone 4D9 GTF2E1 monoclonal antibody (M07A), clone 1E12 HBXIP monoclonal antibody (M02), clone 3D3 HELLS monoclonal antibody (M04), clone 2G10 HMG20A monoclonal antibody (M01), clone 4D5 HNF4A monoclonal antibody (M04), clone 4E2 HSPE1 monoclonal antibody (M01), clone 4C11-B11 ID1 monoclonal antibody (M02), clone 1F7 K-ALPHA-1 monoclonal antibody (M01), clone 4D1 LDB2 monoclonal antibody (M01), clone 1C2 MASA monoclonal antibody (M01), clone 3C1-1D5 MDH1 monoclonal antibody (M01), clone 2B11-B7 MLF2 monoclonal antibody (M01), clone 2F6-1E3 MSN monoclonal antibody (M13), clone 4B8 NANOG monoclonal antibody (M01), clone 2C11 NDUFB11 monoclonal antibody (M08), clone 4B2 Cat. No. 08A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A Description NFYB monoclonal antibody (M01), clone 6H6 NSEP1 monoclonal antibody (M01), clone 4F12 NTS monoclonal antibody (M01), clone 3A11 OIP5 monoclonal antibody (M01), clone 3G10-1D5 PAICS monoclonal antibody (M01), clone 4F4 PGAM1 monoclonal antibody (M01), clone 2G1-A6 PHGDH monoclonal antibody (M01), clone 4A3-1D6 PIPOX monoclonal antibody (M10), clone 3D1 PLTP monoclonal antibody (M01), clone 2F3-G4 POLR2D monoclonal antibody (M01), clone 1E4-A5 PPM1B monoclonal antibody (M01), clone 1A3-2A4 PPP2R2B monoclonal antibody (M01), clone 2C11 PSMB4 monoclonal antibody (M01), clone 6G7-E8 PSMB6 monoclonal antibody (M02), clone S51 PUS1 monoclonal antibody (M01), clone 3A3 RCN1 monoclonal antibody (M01), clone 1F8-E4 RFXAP monoclonal antibody (M01), clone 1B5 RPL39L monoclonal antibody (M01), clone 4A8-1B6 SAE1 monoclonal antibody (M01), clone 1G4-1G5 SAMM50 monoclonal antibody (M04), clone 2A9 SAP18 monoclonal antibody (M01), clone 3B2 SEPT8 monoclonal antibody (M01), clone 1G3-7D1 SERPINH1 monoclonal antibody (M01), clone 1D2-1A6 SOX4 monoclonal antibody (M01), clone 2C5 TCL1B monoclonal antibody (M01), clone 2A12 TCP1 monoclonal antibody (M01), clone 2B2-D6 TGIF monoclonal antibody (M01), clone 1D12 THY1 monoclonal antibody (M01), clone 3F9 TIMM13 monoclonal antibody (M03), clone 4F4 TPT1 monoclonal antibody (M01), clone 3C7 TRIM37 monoclonal antibody (M01), clone 2D11 UBE2L6 monoclonal antibody (M01), clone 2F12-1F4 UGP2 monoclonal antibody (M01), clone 3H3 UNG monoclonal antibody (M01), clone 4C12 VSNL1 monoclonal antibody (M01), clone 2F1-E3 ZNF253 monoclonal antibody (M02), clone 1D7 ZWILCH monoclonal antibody (M01), clone 1C9 Please order online at or call

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