INTRODUCTION TO DSMZ, THE GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES

Transcription

1 INTRODUCTION TO DSMZ, THE GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES

2 TODAY, DSMZ IS A RENOWNED RESEARCH AND SERVICE INSTITUTION WITH COLLECTIONS OF Prokaryotes Yeasts and Fungi Human and Animal Cell Cultures Plant Cell Cultures Plant Viruses and Antisera

3 DSMZ originated from a purely internal research collection of the Institute for Microbiology, University Göttingen 1969 founded as own department Mikrobenbank, later Sammlung von Mikroorganismen Göttingen SMG, on project funding COLLECTION OF BACTERIA, until 1969 SMG

4 1974 Establishment as DSM, a national service collection for the support of the development of national biotechnology; concept of decentralized, specialized collections, housed in thematically related research institution; Göttingen, being the centre with patent deposit and supply services, is institutionally funded. München Gram-positive, non spore forming bacteria Berlin I Phytopathogenic bacteria and fungi Central Göttingen aerobic endospore forming bacteria, anaerobic endospore and non-spore forming bacteria, phototrophic bacteria, patent deposit Weihenstephan Gram-negative Eubacteria Bayreuth Pseudomonadaceae and marine bacteria Berlin II Filamentous fungi, yeasts Darmstadt Actinomycetales

5 DSM INSTITUTE FOR MICROBIOLOGY GÖTTINGEN

6 centralization of 3 of the external bacterial collections; only strains, not staff (number of staff in Göttingen: 4 scientists, 8 technical laboratory assistants, 4 further technical assistants) 1979 transferred as independent department to GBF 1986 establishment of additional work groups (additional 5 scientists and 5 technical assistants); project funding 1987 move to Braunschweig into own new building and centralization of remaining two external bacterial groups (strains and staff) organism groups covered by then: More or less the whole breadth of bacterial and archaeal diversity, aerobic, anaerobic, extremophiles,.; except risk group 3 organisms; smaller range of fungi and yeasts (because of CBS, NL; IMI =CABI Bioscience, UK in Europe)

7 NEW DSMZ BUILDING IN BRAUNSCHWEIG

8 1988 transformed into independent institution under public funding enlargement by additional departments (animal cells, plant cells, plant viruses); on project funding since 1996, DSMZ, after nearly 30 years of instable funding, Blue List Institute, institutional funding, 50% federal government, 50% Länder 1991 Retirement of Director Dr. Dieter Claus Since '93 New Director: Prof. Dr. Erko Stackebrandt

9 DSMZ - General DSMZ - General HISTORY OF DSMZ with regard to its patent depositary 1974 renamed as DSM and recognized by the German patent office and the European Patent Office for microorganisms 1981 status as International Depositary Authority under the Budapest Treaty for bacteria, fungi, yeasts and bacteriophages 1988 status as IDA for isolated plasmid preparations 1990 incorporation of the IDA ZIMET, Jena 1990 status as IDA for plant viruses and plant cell cultures 1991 status as IDA for animal and human cell cultures 1994 status as IDA for murine embryos in all cases material not exceeding RG 2

10 STAFF TODAY DSMZ - General DSMZ - General SCIENTIFIC STAFF 25 PHD LEVEL AND ABOVE BIOLOGISTS, MICROBIOLOGISTS, AGRICULTURAL AND MEDICAL SCIENTISTS TECHNICAL GRADUATED STAFF LABORATORY ~ 25 BTAS, CTAS, MTAS, LTAS OFFICE ~ 14 SECRETARY, INTERNAL ORGANISATION, IMPORT- EXPORT SPECIALISTS, PUBLIC RELATION, COMPUTER SPECIALISTS TECHNICAL NON GRADUATED STAFF ~4 GENERAL HELP, CLEANING GLASS WARE DIPLOMA AND PhD STUDENTS, POST DOCS, GUEST RESEARCHERS

11 Collecting, maintaining and supplying biological diversity of microorganisms, cell cultures and plant viruses Research in collection related areas, publication activities, cooperation in research areas (e.g. as editors and reviewers in relevant journals, in ICSP, as consultants, etc.) Worldwide cooperation with other collections and in international organisations (e.g. WFCC, ECCO, CABRI, EBRCN, UNESCO, GBIF, EUROCAT, ENBI, OECD-BRC, etc.) DSMZ is the national GBIF Node for Prokaryotes and Viruses

12 STORAGE OF FREEZE-DRIED AMPOULES Microbiology Microbiology

13 LIQUID NITROGEN STORAGE Microbiology Microbiology

14 Microorganisms Microorganisms MANAGEMENT OF RESOURCES: CLOSE INTERLINKAGE OF RESEARCH AND SERVICE IN THE DEPARTMENT OF MICROBIOLOGY NEW ACCESSIONS ENLARGERMENT OF THE COLLECTION COLLECTION RELATED RESEARCH IDENTIFICATION RECULTIVATION REPLENISHMENT OF STOCKS AUTHENTICATION

15 Phase Contrast Microscope; standard magnification: x40; x100 Dissecting Microscope; standard magnification: x0,8 x2

16 VIEW OF LABORATORY Microbiology Microbiology

17 BIOCHEMICAL TEST SYSTEMS Microbiology Microbiology

18 INCUBATOR ROOM Microorganisms Microorganisms

19 MOLAR G+C CONTENT OF DNA Identification Identification

20 DNA DNA HYBRIDIZATION Identification Identification

21 DNA ISOLATION Identification Identification

22 16S rrna SEQUENZING Identification Identification

23 RIBOPRINTER Identification Identification

24 ONE AND TWO DIMENSIONAL TLC FOR CELL WALL ANALYSIS Identification Identification

25 DISPATCH DEPARTMENT Microbiology Microbiology

26 Quality Management at DSMZ Implementation of an International Recognised Quality Standard covering Management of Expertise and Protection of Diversity: DIN EN ISO 9001:2000

27 Expectations from and role in Demonstration Project Expectations - transfer in-house procedures into harmonized SOP - fill gaps identified during comparison of in-house and OECD procedures (e.g. risk assessment) - develop audit scheme for OECD guidelines along ISO - develop training schemes - using synergy effect for working towards a national network of BRCs Role - Dagmar Fritze funded for Demonstration Project central secretariat (BMBF, DSMZ) - output from DSMZ projects (e.g. EMbaRC, training) or internal work provided to workpackages (e.g. lessons learnt from ISO 9000 implementation) - international contacts (e.g. regional input from ECCO)

28 THANK YOU!

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