Rules / Gameplay. Home Run Derby Everyone stands, get right answer stay standing, wrong answer you sit down. Last person standing wins! Prize =??

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2 Rules / Gameplay Each batter gets asked a question. Correct answers are considered hits and you move to the appropriate base. Runs are scored by advancing base runners. Outs are made by answering a question wrong. If the other team gets the right answer, counts as a double play = 2 outs Team with most runs wins. Home Run Derby Everyone stands, get right answer stay standing, wrong answer you sit down. Last person standing wins! Prize =??

3 A loss of 10% of the body's water can result in death.

4 Which of the following is a way to permanently identify a laboratory animal? Cage cards Ear punches Colored markers Collars and tags Naming them

5 Using a barrier to prevent the entry of infectious agents into a room is called Biocontainment

6 Amino acids join together to form:

7 Information on how to safely handle and work with a substance can be found where?

8 What does sterilization do? Washes away microorganisms Reduces the number of organisms on a surface to an acceptable public health standard Increases the sale of frozen peas Destroys organisms on an object or surface Destroys all organisms on, and sometimes inside, an object

9 The Guide recommends a room ventilation rate of how many air changes per hour? 1 to 4 5 to 9 10 to to 20 None of the above

10 What 2 types of molecules join together to make up carbohydrates?

11 Name the head football coach at The Ohio State University.

12 Acid and are sometimes used to treat the water in animal facilities to prevent the growth of pathogens.

13 What is the typical flow of caging and equipment through a cage wash area? Dirty side to cage washer to clean side to animal room Animal room to clean side to cage washer to dirty side Clean side to cage washer to dirty side Cage washer to dirty side to clean side to animal room Dirty side to cage washer to locker room to Laine s office

14 What are the four common types/groups of microorganisms found in an animal facility?

15 What is the room called that is intended to separate incoming animals from the existing colony? Quarantine room Bio exclusion room Isolation room 3 rd door on your right Biosafety room

16 Why must you remove organic matter such as hair, saliva, feces, BEFORE disinfecting a surface?

17 Vitamin C is an essential nutrient for which species? Albino animals Rats and ferrets Gerbils and cats Guinea pigs and nonhuman primates Hamsters and sheep

18 Rabbit rooms are usually kept warmer than rodent room

19 Bacteria that has formed a tough outer coat for protection against harsh environmental conditions

20 Skin tenting is used to check for what problem? Dehydration Anemia Anorexia Hypothermia Pregnancy

21 How often should automated watering systems be checked to ensure they are in proper working order?

22 What is superheated steam? Autoclaves

23 Pesticides in animal facilities should be: applied often to reduce potential contamination of feed storage rooms. Should not be used, roaches are Jen s friends!! used only in carcass storage areas. strictly controlled and applied only by skilled and experienced personnel. used only in shipping and receiving areas.

24 O-H

25 Match the equipment with its function: Class II BSC Determines weights Incinerator Protects user from chemical fumes Fume Hood Protects user from pathogenic microorganisms Autoclave Burns material such as animal tissues and trash Balance Sterilizes instruments, caging, equipment etc.

26 How is a ruminants digestive system different? Needed to digest diet (fiber, roughage)

27 Which nutrient is considered the most important and comprises more than two thirds of the body by weight?

28 Proteins are large, complex molecules.

29 Name 2 design features of ideal walls/ceiling for an animal room.

30 What is the definition of disinfection? To reduce the number of organisms on a surface to an acceptable public health standard To destroy all organisms on, and sometimes inside, an object To destroy microorganisms or affect their growth on an object or surface. To wash away microorganisms All of the above

31 What are the three processes used to either remove or kill microorganisms? Sanitization, disinfection, sterilization Being forced to watch Dora the Explorer over and over and over again! Sampling, disinfection, depopulation Removal, sanitization, sterilization Sanitization, desensitization, sterilization

32 In the case of a malfunction, which type of watering system has the most potential to drown rodents? A cup of water on the floor of the cage A gel hydration pack An automatic watering system A water bottle The Great Flood

33 Hay and straw are the same thing?

34 What is the most vital piece of information that MUST be on every cage card if the animal is in a research study?

35 Feed is much more important than water for the survival of an animal.

36 Only disinfectants labeled as sporicidal will kill spores.

37 Which of the following would be an emergency situation? The royal baby had a bowel movement An escaped nonhuman primate A missing cage card A dead rat found in a cage A water bottle that leaked and soaked a mouse cage

38 Which of the following is TRUE about certified feed: The manufacturer has certified that only a certain percentage of moisture is present in the feed. The manufacturer certifies that the feed has been irradiated. The manufacturer certifies that specific medications have been included in the feed. The manufacturer certifies that contaminants are below a certain level. The manufacturer certifies that the food is recommended by 9 out of 10 mice in a nationwide taste test!!

39 Match vitamin/mineral on left with description on right: Vitamin C Fat soluble vitamins Iron Deficiency can lead to thinning of the bones Calcium Sodium & chloride Cannot be synthesized by NHP or guinea pigs Deficiency can lead to anemia Vitamin A, D, E and K Electrolytes.

40 What are design feature of ideal doors for an animal room All of the below Self-closing kickplates locks Recessed handles

41 What is the acceptable range of humidity within an animal room?

42 How does a RODAC plate test the effectiveness of a sanitization program?

43 What is YOUR microenvironment right now?

44 What type of caging is NOT recommended by the GUIDE for rats and mice?

45 Fiber is a type of carbohydrate.

46 What nonchemical methods are often used to reduce contamination in animals drinking water? Reverse osmosis and ultraviolet radiation Heating to boiling and cooling Adding only the finest vodka High-voltage electrification and grounding Acidification and chlorination

47 The primary enclosure refers to: The animal s cage The rack holding the animal s cage The animal room The shipping crate the animals originally arrived in The animal facility

48 What should you do if you find that the temperature in a room has fallen below the accepted range? Notify the environmental health and safety department. Notify your supervisor. Write your congressman. Write the temperature that it should be on the room check sheet. Notify THE Justin Lahmers

49 What is the food type and the milling date?

50 In which piece of equipment should you open bottles of volatile chemicals? A Class II BSC A fume hood A Class III BSC A deep well sink to avoid splashes A cage changing station

51 What specific types of animals are especially light sensitive

52 What type of diet contains drugs for treating animals? Guaranteed analysis diet Medicated Diet Irradiated Diet Certified diet Standard diet

53 Who is the best review session instructor to date?

54 The final rinse temperature of the cage wash cycle is 212 F

55 What is ventilation? The rate at which room air is exchanged with fresh air The use of positive and negative air flow The temperature control for an animal s microenvironment The air pressure of a room Respirator fit testing

56 What term refers to the environmental conditions within a room holding animals? Microenvironment Isoenvironment Macroenvironment Claustrophic Toxic environment

57 Cage changing is NOT a significant stressor for animals.

58 What does the abbreviation HEPA stand for?

59 Whenever possible, social animals should be housed: With Marvin Gaye playing in the background and the lights out In pairs of groups individually In the smallest cages available With animals of the opposite sex

60 Housing for what species requires a raised resting platform, a clean litter pan, and fresh litter daily?

61 Match the activity with appropriate location in the vivarium: Break room Change cages Locker room Euthanize a cage of mice Procedure room Eat a snack Survival surgery room Change into scrubs Animal housing room Sterile surgery on a NHP

62 What are 2 common species that do NOT get fasted prior to surgery? They are not able to vomit

63 How does a biological safety cabinet protect the user from pathogens or allergens the animal inside of it may have?

64 Who is the best facility supervisor?

65 Appropriate caging should balance the needs of the animal with: the ability to provide for sanitation. the ability to store cages properly in the facility. the needs of the animal care staff. The cost of the caging The odors generated in the room

66 How often should technicians assess animal health?

67 What is the most commonly used metal in laboratory animal caging? Type 304 and 316

68 The two species most commonly found in aquatic cages in a laboratory setting are?

69 What can happen to the animals if ventilation fails in an individually ventilated cage? The animals can die from high carbon dioxide levels. Microorganisms can escape into the environment. Barbering The animals can develop a disease due to the low humidity. The animals will stop eating and will require supplementation.

70 Match the type of disinfectant with its intended purpose: Virostatic Kills bad breath Virucidal Kills bacteria Bacteriocidal Inhibits growth of viruses Bacteriostatic Kills viruses Listerine Inhibits growth of bacteria

71 If air rushes out of the room when you open the door, the room pressure is set to negative.

72 What type of cages are nonhuman primates often housed in to facilitate restraint for brief procedures? Transfer cages Squeeze cages Metabolic cages Shoebox cages No cage they sit on your shoulder

73 Animals with which disease have increased urine output and increased water consumption? Scurvy Rickets Diabetes Mike Rowley Syndrome Anemia

74 What document contains space recommendations for most laboratory animals?

75 Biohazard studies are typically conducted in a room with what type of pressure? keep the hazardous agents or pathogens contained to the room

76 Matching : Cologne, jewelry Specify minimum sanitation standards USDA, AAALAC Should be scrubbed thoroughly Animal bites Escaped mouse that was caught outside of hood Should not be worn in vivarium Can review husbandry records, site visits Animal Welfare regulations Should not be placed back in colony

77 Which acronym refers to withholding both food and water from an animal?

78 Why is feed offered in more than one location for group-housed animals?

79 Who is going to easily pass the ALAT exam?