Endomorphin-1 and Endomorphin-2. These items may be or may be not in stock anymore. Please contact us, if you are interestet in purchasing.

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1 These items may be or may be not in stock anymore. Please contact us, if you are interestet in purchasing. Cyclic Nucleotide Antisera Antiserum against Cyclic AMP ( rabbit ) lyophilized RIA-Titer: 1 : Crossreactivity: < % ATPcGMP % Antiserum against Cyclic GMP ( rabbit ) lyophilized RIA-Titer: 1 : A-001 Crossreactivity: GTP < % camp < % 4G-101 Endomorphin-1 and Endomorphin-2 Endomorphin-1 ( Tyr-Pro-Trp-Phe-NH2 ) MW: purity > 97 % (HPLC) Endomorphin-2 ( Tyr-Pro-Phe-Phe-NH2 ) MW: purity > 97 % (HPLC) P-600 P-610

2 G-Protein Antisera (alpha,, gamma) The following polyclonal antisera were raised in rabbits against peptide sequences specific for G-protein subunits. All antisera are suitable for use in Western Blotting and are provided as lyophilized sera. Their specificity was verified by blocking antibody binding with the corresponding peptide antigens. The quantities supplied are sufficient for 100, 200 or 500 ml, respectively, when using the antisera at the indicated dilution. Subunit Peptide Binding Site Specifity Titer (blot) alpha common GAGESGK- STIVKQMK NLKEDGIS- AAKDVK GTP-binding core Aminoacids of 1 SKNRSPN Aminoacids of 1 1/2 2, 1-3 alpha-1 >> 1 2 KNNLKDCGLF 1, alphai2, alpha-t >>3 EEQGML- PEDLS Aminoacids of 2 3 IDFGEAARAD Aminoacids alpha-p also termed alpha-z or alpha-x alphaq=11 alpha-12 alpha-13 alpha-14-2 RMHLRQ- YELL RMHLRQ- YELL TPEPGED- PRVTR QNNLKY- IGLC QLNLKE- YNLV QENLKD- IMLQ HDNLKQ- LMLQ QLNLRE- FNLV EGNVRVSR- ELAGHTGY CGDSTLTQ- ITAGLD gamma-1 CEEFRD- YVEER 3rd Loop 1 : A-100 1: A : A : A : A : A-145 short long short long short long 1 : A : A : A-160 alpha-p/z/x 1 : A-170 alpha-q alpha-11 1 : A-180 alpha-12 1 :1000 3A-185 alpha-13 1 :1000 3A-190 alpha-14 1 :1000 3A-195 Aminoacids of -1 Aminoacids of -2 Aminoacids 8-18 of gamma : B : B-220 gamma-1 (Transducin 1 : G-100

3 gamma) gamma-2 CEAHA- KED gamma-3 MKGETP- VNSTSI gamma-5 FRPQ- KVCSFL Aminoacids of gamma-2 Aminoacids 1-12 of gamma-3 Aminoacids of gamma-5 gamma-2 1 : G-200 gamma-3 1 : G-300 gamma-5 1 : G-500 G-Protein Antisera Set Quantity This set contains a collection of 5 vials of our Anti-G-Protein Antisera, each sufficient for 25 ml of incubation solution. Antisera: alpha-common 5 microliter ( : 3A-100 ) Or any other 2 microliter ( : 3A-110 ) 1/2 4 microliter ( : 3A-130 ) collection of 1 microliter ( : 3A-150 ) 7 microliter ( : 3B-200 ) 5 antisera 6K Set

4 G-Protein Peptide Fragments Subunit alpha-t alpha-p alpha-q=11 gamma-3 Cys-GAGESGKSTIVKQMK, GTP-binding core, acomm Cys-Gly-Ala-Gly-Glu-Ser-Gly-Lys-Ser-Thr-Ile-Val-Lys-Gln-Met-Lys Aminoacids of //alpha-t MW Cys-NLKEDGISAAKDVK, Aminoacids of, Cys-Asn-Leu-Lys-Glu-Asp-Gly-Ile-Ser-Ala-Ala-Lys-Asp-Val-Lys MW LKDCGLF, of 1 or 2 Leu-Lys-Asp-Cys-Gly-Leu-Phe MW EEQGMLPEDLS, Aminoacids of 2 Glu-Glu-Gln-Gly-Met-Leu-Pro-Glu-Asp-Leu-Ser MW Cys-KQLQKDKQVYRATHR, Aminoacids of Cys-Lys-Gln-Leu-Gln-Lys-Asp-Lys-Gln-Val-Tyr-Arg-Ala-Thr-His-Arg MW Cys-TPEPGEDPRVTR-amide, Aminoacids of Cys-Thr-Pro-Glu-Pro-Gly-Glu-Asp-Pro-Arg-Val-Thr-Arg-amide (3rd loop) MW RMHLRQYELL, Aminoacids of Arg-Met-His-Leu-Arg-Gln-Tyr-Glu-Leu-Leu < 80% pure by HPLC MW QNNLKYIGLC, Aminoacids of alpha-p Gln-Asn-Asn-Leu-Lys-Tyr-Ile-Gly-Leu-Cys < 80 % by HPLC MW CQLNLKEYNLV, Aminoacids of alpha-q Cys-Gln-Leu-Asn-Leu-Lys-Glu-Tyr-Asn-Leu-Val 85 % pure by HPLC MW Cys-EGNVRVSRELAGHTGY, Aminoacids of -1 Cys-Glu-Gly-Asn-Val-Arg-Val-Ser-Arg-Glu-Leu-Ala-Gly-His-Thr-Gly-Tyr MW 1848 MKGETPVNSTI-Cys-amide, Aminoacids 1-12 of gamma-3 Met-Lys-Gly-Glu-Thr-Pro-Val-Asn-Ser-Thrr-Ile-Cys > 70 % pure by HPLC MW

5 Immuno-affinity-gels for Immunoprecipitation of G- Proteins -Immunoprecipitation-gel, lyophilized 10 mg of protein A-IgG of antiserum 3A-150 coupled per ml beads. 15 microliter of the beads will bind (short and long) of microgramm of membrane protein. alpha-p-immunoprecipitation-gel, lyophilized 10 mg of Protein A-IgG of antiserum 3A-170 per ml beads. 20 ml of the beads will bind ap of mg of membrane protein 3I-154 3I-174 myu- and delta-opioid-receptor derived Peptide Fragments Loop MOR-1A C.-term. delta, 1. ex-tern loop L E N L E A E T A P L P > 90% pure by HPLC MW M E T W P F G E L L - amide > 80% pure by HPLC MW 1221 R-600 R-500 Nociceptin / Orphanin FQ - Antiserum The antiserum was raised in rabbits, it was tested in ELISA and for its application in immunohistochemitry. Specificity was verfied by blocking antibody binding with nociceptin Nociceptin- Antiserum, Immunohist. titer 1 : Nociceptin ( FGGFTGARKSARKLANQ ) MW 1809 OP-500 P-550 Nociceptin/orphaninFQ and opioid peptides show overlapping distribution but not colocalization in pain-modulatory brain regions, Stefan Schulz, Matthias Schreff, Dora Nüß, Christian Gramsch and Volker Höllt (1996) NeuroReport 7, Last modfied October 1st Silvia Kulzer & Johannes Gramsch