Cell Health. Cell Health Range. Antibodies, kits, and viability dyes

Transcription

1 Cell Health Cell Health Range Antibodies, kits, and viability dyes

2 Cell Health Overview CELL HEALTH No experiment is complete without all its components and one key component is confidence in the health of your cells. Determining the health of your cells is a crucial component in ensuring reproducibility of your experiments. Whether you are assessing viability of cells in culture, cell proliferation in response to different stimuli, are trying to understand which treatments induce apoptosis or autophagy, or simply want to improve your flow cytometry data, we have the right reagent for you. Our years of experience and extensive portfolio of reliable reagents ensure that you have the right reagent for every experiment and can collect and interpret your data with confidence. CELL CULTURE Cell Viability Measure general cell health by monitoring parameters such as redox potential and membrane integrity. EXPERIMENTAL TREATMENT Cell Proliferation Quantify cell divisions using antibodies against key proliferation markers or by using thymidine analogs and dyes. Autophagy Track degradation of cytoplasmic components and autolysosome formation with stage-specific kits and antibodies. Apoptosis Distinguish the stages of programmed cell death using assays or antibodies for stage-specific markers. 2

3 Cell Viability Cell Viability Determining cell viability is vital for many experiments. It can be the data in toxicity assays, be used to determine side effects of experimental treatments, provide accurate information about cellular activity, or simply be used to ensure that you are studying a live cell population. We offer a range of versatile and easy-to-use cell viability assays, including convenient fixable dyes that allow you to measure the viability of cells using flow cytometry, microscopy, and ELISA. Featured : VivaFix Cell Viability Assays Fixable cell viability assays can be used to easily discriminate dead cells from live cells in both fixed and unfixed samples using flow cytometry. Assays are available in a range of excitation and emission spectra for convenient addition to multicolor flow cytometry panels (see table below). A B SSC area SSC area VivaFix 498/521 VivaFix 498/521 Fig. 1. Live and dead cells are easily distinguished in fixed and unfixed samples using VivaFix Cell Viability Assays. Jurkat cells were stained with the VivaFix 498/521 Cell Viability Assay, fixed with 3.7% formaldehyde (A) or left unfixed (B), and then analyzed using the S3 Cell Sorter. SSC, side scatter Viability and Nuclear Dyes Applications Excitation Emission Optimal Excitation Laser Catalog Number alamarblue E, FN, IF/ICC N/A N/A N/A BUF012A/BUF012B ReadiDrop Pl FC, IF or ReadiDrop 7-AAD FC, IF or VivaFix 353/442 FC VivaFix 410/450 FC VivaFix 408/512 FC VivaFix 398/550 FC VivaFix 498/521 FC VivaFix 547/573 FC VivaFix 583/603 FC VivaFix 649/660 FC PureBlu DAPI Nuclear Staining Dye IF or PureBlu Hoechst Nuclear Staining Dye IF, FC Go to bio-rad-antibodies.com/cell-health to view our full product range. 3

4 Cell Proliferation Cell Proliferation An increase or decrease in cell growth and division can be a key result in cytotoxicity and apoptosis assays and can be used to determine the effect of experimental treatments on cellular replication. We offer a broad range of antibodies, kits, and dyes to monitor proliferation. Proliferation can be measured through dye incorporation into live cells, by quantifying key proliferation markers, or by assessing incorporation of the thymidine analog BrdU into DNA. Featured : CytoTrack Cell Proliferation Assays CytoTrack Dyes allow you to measure cell proliferation in live cells. As the labeled cells divide the concentration of the dye is halved. Proliferation can be measured and cell generations distinguished using this cell-division dependent decrease in fluorescence. CytoTrack Dyes are available in a range of excitation and emission spectra for convenient incorporation into multicolor flow cytometry panels. Normalized to mode Fig. 2. CytoTrack Cell Proliferation Assays are able to resolve up to 10 cell generations. CytoTrack Green 511/525 Proliferation Dyes Applications Excitation Emission Optimal Excitation Laser Catalog Number alamarblue E, FN, IF/ICC N/A N/A N/A BUF012A/BUF012B CFDA-SE FC CytoTrack Blue 403/454 FC CytoTrack Green 511/525 FC CytoTrack Yellow 542/556 FC CytoTrack Red 628/643 FC Proliferation Antibodies Clone Species Reactivity Applications Host Formats Catalog Number ** BrdU BU1/75 (ICR1) FC*, IF/ICC, Biot., FITC Rat Pur., A488, Biot., FITC MCA2060 BrdU Bu20a FC*, IHC-F, IHC-P* Mouse Pur., FITC MCA2483 Ki Human E, IHC-P* HuCAL Pur. HCA117 Ki Bovine, dog, human Ki (Fab) Bovine, dog, human MCM2 PrecisionAb Antibody 3B6 Human, mouse, rat E, IHC-F, IHC-P*, WB HuCAL Pur. HCA053 E, IF/ICC, IHC-F, IHC-P*, WB HuCAL Pur. HCA006 WB Mouse Pur. VMA00259 MCM2 Monoclonal CRCT2.1 Human IHC-F, IHC-P*, WB Mouse Pur. MCA1859 PCNA PrecisionAb Human, mouse, rat WB Mouse Pur. VMA00018 PCNA Monoclonal PC10 Broad FC*, IHC-F, IHC-P, IP, WB Mouse Pur., FITC, HRP PCNA Polyclonal IG7 Bovine, dog, human, mouse, rat MCA1558 E, IHC-P, WB Rabbit Pur. AHP Go to bio-rad-antibodies.com/cell-health to view our full product range.

5 Autophagy Autophagy Autophagy is the orderly degradation and recycling of cytoplasmic components via the lysosome. Autophagy can be induced by factors such as starvation or growth factor suppression, ER stress, infection, or through immune signals. Autophagy is thought to be a repair and survival response during stress and infection and has been linked closely to apoptosis. We offer a range of antibodies and kits to detect and measure markers of autophagy. These include reagents to detect the lysosome, the autophagosome, and the most-studied macroautophagy pathway. Visit abdserotec.com/autophagyantibodies for more information and to view a more comprehensive list of available autophagy antibodies. Featured : MAP1LC3A/B Antibody MAP1LC3 family proteins are widely used as reliable markers for monitoring autophagy. LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during autophagy. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity. The rabbit anti human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B in immunofluorescence and western blot experiments. Fig. 3. Autophagy can be monitored by immunofluorescence using AHP2167, a MAP1LC3A/B antibody. HeLa cells were fixed with 3% paraformaldehyfe and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a rabbit anti human polyclonal MAP1LC3A/B antibody at a 1:25 dilution. STAR36D488GA, a sheep anti rabbit DyLight 488 antibody was then used at 1:50. Cells were visualized on the Bio Rad s ZOE Fluorescent Cell Imager at 60x magnification. Kits Applications Catalog Number Magic Red Cathepsin B Kit IF/ICC ICT937 Magic Red Cathepsin K Kit IF/ICC ICT939 Magic Red Cathepsin L Kit IF/ICC ICT941 Autophagy Antibodies Clone Species Reactivity Applications Host Catalog Number Beclin-1 Polyclonal Human, mouse, rat FC, IHC-P*, WB Rabbit AHP1009 MAP1LC3A/B (N-Terminal) Polyclonal Human IF, WB Rabbit AHP2167 CD107a (LAMP-1) Polyclonal Human, mouse, rat IHC-P* Rabbit AHP1742 CD107a (LAMP-1) B-T47 Mouse FC, IHC-P Mouse MCA2881 CD107a (LAMP-1) 1D4B Mouse FC, IHC-F, IF/ICC, IP CD107a (LAMP-1) 4E9/11 Pig FC*, IHC-F, IF/ICC, IHC-P*, IP, WB Rat Mouse MCA4707 MCA2315 CD107b (LAMP-2) Polyclonal Human, mouse, rat WB Rabbit AHP2324 CD107b (LAMP-2) Polyclonal Human, mouse, rat E, ICC, WB Rabbit AHP2325 CD107b (LAMP-2) Polyclonal Human, mouse, rat IHC-F, WB Rabbit AHP1634 CD107b (LAMP-2) M3/84 Mouse FC, IHC-F, IHC-P, IP, WB Mouse MCA2293 CD107b (LAMP-2) AC17 Human, dog FC*, IF/ICC, IP, WB Mouse MCA2558 5

6 Apoptosis Apoptosis Apoptosis is the process of programmed cell death characterized by distinct morphological and biochemical changes within a cell. We provide easy-to-use apoptosis kits, annexin V reagents, and antibodies against common apoptosis markers to allow determination of cellular apoptosis levels. Visit abdserotec.com/apoptosis-autophagy for a full list of all of our apoptosis products. Featured s: Mitochondrial Membrane Potential Kit and Bcl-2 PrecisionAb Antibody An early indication of apoptosis involves a collapse of the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5,6,6 -tetrachloro-1,1,3,3 -tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into our MitoPT JC-1 Mitochondrial Permeability Transition Kit. Bcl-2 is a member of a family of anti-apoptotic proteins that prevent the release of sequestered mitochondrial cytochrome c and subsequent caspase 9 and 3 activation to promote cell survival. A B Fig. 4. Apoptosis can be monitored using the MitoPT JC-1 Mitochondrial Permeability Transition Kit and Bcl-2 PrecisionAb Antibody. A, Jurkat cells were stained using the MitoPT JC-1 Kit and imaged using a fluorescence microscope. Non-apoptotic cells exhibit red stained mitochondria (two cells at the top). Apoptotic cells with varying stages of mitochondrial membrane potential collapse appear green (3 cells at the bottom). B, Western blot analysis of whole cell lysates probed with Bcl-2 PrecisionAb Antibody followed by detection with HRP-conjugated goat anti mouse IgG (1/10,000, STAR207P). Arrow points to Bcl-2. Kits 6 Applications Excitation Emission Optimal Excitation Laser APO-BrdU Flow Cytometry Kit FC, IF/ICC 490 and and APO001 APO-BrdU-IHC Immunohistochemistry Kit IHC-F, IHC-P N/A N/A N/A APO002 Catalog Number Annexin V: Biotin Assay Kit FC N/A N/A N/A ANNEX20B/100B/300B Annexin V: FITC Assay Kit FC ANNEX20F/100F/300F Annexin V: PE Assay Kit FC ANNEX50PE/200PE Annexin V: APC Assay Kit FC ANNEX50APC/200APC FAM FLICA Caspase-1 Kit FC, IF/ICC ICT097 FAM FLICA Caspase-10 Kit FC, IF/ICC ICT923 FAM FLICA Caspase-2 Kit FC, IF/ICC ICT918 FAM FLICA Caspase-6 Kit FC, IF/ICC ICT095 FAM FLICA Caspase-8 Kit FC, IF/ICC ICT099 FAM FLICA Caspase-9 Kit FC, IF/ICC ICT912 FAM FLICA Caspase-3/7 Kit FC, IF/ICC ICT093 FAM FLICA Poly Caspase Kit FC, IF/ICC ICT091 SR FLICA Caspase-9 Kit FC, IF/ICC ICT960/ICT961 SR FLICA Poly Caspase Kit FC, IF/ICC ICT916 Magic Red Caspase-3/7 Kit IF/ICC N/A N/A N/A ICT935 Mitochondrial Membrane Potential Kit FC, IF/ICC or 561 ICT946/ICT948 Mitochondrial Permeability Transition Kit FC, IF/ICC and ICT943/ICT944

7 Apoptosis Antibodies Apoptosis Antibodies Clone Species Reactivity Applications Host Formats Catalog Number ** Annexin V VAA-33 Human E, FC Mouse Pur., Biot., FITC MCA2712 Caspase-1 Polyclonal Human IHC-P*, WB Rabbit Pur. AHP963 Caspase-1 3D2 Human IHC-P* Mouse Pres. Fr. MCA2979Z Caspase-2 1C10 Human E, WB Mouse Pur. MCA2563 Caspase-2 Polyclonal Human, mouse IHC-P*, WB Rabbit Pur. AHP1423 Caspase-3 Polyclonal Human IHC-P*, WB Rabbit Serum AHP476 Caspase-4 Polyclonal Human, mouse IHC-P*, WB Rabbit Pur. AHP964 Caspase-5 Polyclonal Human WB Rabbit Pur. AHP965 Caspase-7 Polyclonal Human, mouse, rat IHC-P*, WB Rabbit Pur. AHP1788 Caspase-7 Polyclonal Human, mouse, rat IHC-P*, WB Rabbit Pur. AHP966 Caspase Human E, WB Mouse Pur. MCA2739 Caspase-8 Polyclonal Human, mouse, rat WB Rabbit Pur. AHP967 Caspase-9 Polyclonal Human, mouse, rat WB Rabbit Pur. AHP492 Caspase-9 Polyclonal Human, mouse, rat E, WB Rabbit Pur. AHP491 Caspase-10 Polyclonal Human, mouse, rat WB Rabbit Pur. AHP2284 Caspase-12 Polyclonal Human, mouse, rat IHC-P*, WB Rabbit Pur. AHP625 Caspase-12 Polyclonal Human, mouse, rat IHC-P*, WB Rabbit Pur. AHP626 Caspase-13 Polyclonal Human, mouse, rat WB Rabbit Pur. AHP582 Caspase-14 4C9 Human IF, WB Mouse Pres. Fr. MCA3778 Caspase-14 Polyclonal Human, mouse, rat WB Rabbit Pur. AHP1175 Caspase-3 ACTIVE Polyclonal Human, mouse, rat IHC-P, WB Rabbit Pur. AHP2286 Caspase-7 ACTIVE Polyclonal Human, mouse, rat IHC-P, IP, WB Rabbit Pur. AHP2287 Caspase-9 ACTIVE Polyclonal Human IP, WB Rabbit Pur. AHP2288 Caspase-9 ACTIVE Polyclonal Human, rat IP, WB Rabbit Pur. AHP2289 Cytochrome C 2G8 Human E, FC* Mouse Pur. MCA2740 Bcl-2 PrecisionAb Human WB Mouse Pur. VMA00017 Bcl Human FC*, IHC-F, IHC-P*, IP, WB Mouse Pur. MCA1550 Bcl-2 Polyclonal Human E Rabbit Pur Bcl-2 Polyclonal Human E Rabbit Pur Bcl-2 Polyclonal Human, mouse, rat E, WB Goat Pur. AHP1057 CD95 LOB 3/17 Human FC, IP Mouse Pur., A647, FITC, RPE MCA1539 CD95 Polyclonal Human, mouse, rat IHC-P, WB Rabbit Pur. AHP2306 CD178 14C2 Human E, FC, IP Mouse Pur., A488, A647, RPE MCA2409 CD178 MFL4 Mouse, rat FC, FN Hamster Pur., A488, A647, FITC, LE, RPE MCA2896 CD178 10F2 Human E, FC, FN, IP Mouse Pur., EL MCA2408 Granzyme B GB7 Human IHC-P*, WB Mouse Pur. MCA2118 Granzyme B GB10 Human E Mouse Biot. MCA2119 Granzyme B GB11 Human E, FC*, IHC-F, IF/ ICC, IP Mouse Pur. MCA2120 Abbreviations: Axxx, Alexa Fluor; Biot., biotin; E, ELISA; FC, flow cytometry; FN, functional assay; IF/ICC, immunofluorescence/immunocytochemistry; IHC-F, immunochemistry frozen (cryostat); IHC-P, immunohistochemistry paraffin; IP, immunoprecipitation; LE, low endotoxin; Pres. Fr., preservative free; WB, western blotting * Requires special treatment. Refer to product datasheet. ** Refer to the website for each format-specific catalog number. Go to bio-rad-antibodies.com/cell-health to view our full product range. 7

8 Application Resources - Practical Help and Advice Comprehensive application guides Detailed information on experimental controls Easy-to-follow protocols Hands-on tips and tricks to help you improve and optimize your experiments Visit abdserotec.com/applications to find out more. Visit bio-rad-antibodies/cell-health for more information. Bio-Rad Laboratories, Inc. Life Science Web site Group Americas Toll free (USA & Canada) Americas antibody_sales_us@bio-rad.com Europe antibody_sales_de@bio-rad.com UK & Worldwide antibody_sales_uk@bio-rad.com LIT.CH. V Copyright Bio-Rad Laboratories, Inc. All rights reserved. Published by Bio-Rad, Endeavour House, Langford Lane, Langford Business Park, Kidlington, OX5 1GE. Bio-Rad reagents are for research purposes only, not for therapeutic or diagnostic use. alamarblue is manufactured for AbD Serotec, patent 5,501,959 and is a registered trademark of Trek Diagnostic System. U.S. Alexa Fluor is a registered trademark of Molecular Probes, Inc, OR, USA, HuCAL is a trademark of MorphoSys AG, APO-BrdU is a trademark of Phoenix Flow Systems, USA. FAM is a trademark of Applera Corporation. FLICA and MitoPT is a trademark of Immunochemistry Technologies Inc, USA.