Portable Biomedical Diagnostic Inspection System using BioCMOS technology

Transcription

1 1 Portable Biomedical Diagnostic Inspection ystem using BioCMO technology BioCMO Office Japan & ingapore Phone

2 7.49mm Booth #33 (Nagoya University) BioCMO Portable Biomedical Diagnostic Inspection ystem using BioCMO technology 6.97mm 64x64 sensor array BioCMO chip electrochemical sensor potentiometric amperometric impedimetric 2-dimmensional image of biomedical responses applications home healthcare gene-based diagnostic virus detection food security evidence-based care drug discovery

3 Electrochemical Biosensor using Large cale Integrated Circuit 3 logging stand-alone handheld low noise battery operation temperature control median temperature recording to D memory card 2-dimmensional image of biomedical responses

4 principle of electrochemical biosensor 4 recognition + transducer molecule to be detected specific molecular interaction molecular function probe molecule electric signal information communication electrochemical measurement potentiometric amperometric impedimetric

5 5mm ensor structure 5 5mm PDM electrolyte Post CMO process bead U-8 self-assembled monolayer Au polyimide standard CMO process i substrate p metal insulator p + p + n + n + n-well schematic cross-section target (to be identified) probe (enzyme, antibody, primer, ) plan view (photomicrograph) of sensor array

6 ensibility [mv/decade] Detected voltage [mv] detected voltage [mv] Detected glucose [mg/dl] Detected glucose concentration [mm] Application I Glucose ensor H. Anan, M. Kamahori, Y. Ishige, and K. Nakazato, ensors and Actuators B: Chemical 187, , 213 H. Komori, K. Niitsu, J. Tanaka, Y. Ishige, M. Kamahori, and K. Nakazato, BIOCA diabetic Potential diabetic normal hypoglycemia [Red]/[Ox] =1-3 ~ Buffer ion concentration (NaCl) [mm] (4.2%) (5.%) wide buffer condition (5.7%) ( ) variation Glucose in human serum [mg/dl] ph [mg/dl] detection limit 1 m M (.18mg/dL) Given glucose concentration [mm] Glucose Galactose Maltose time [sec] selectability

7 Potential [mv] Change in potential [mv] Application II Ox Red gene-based diagnostic target DNA G A A A C T T T probe DNA primer polymerasation dntp (datp/dttp/dctp/dgtp) 7 Au i PPi + H 2 O PPase 2Pi GAPDH Pi+GAP+ NAD 1,3BPG + NADH Diaphorase 2[(CN) 6 ] 3- + NADH 2[(CN) 6 ] 4- + NAD mv/decade PPi concentration [mm] mv dctp (mismatch) dttp (match) Time [s] H. Ishihara, K. Niitsu, and K. Nakazato, DM214

8 Current [na] Current [na] Application III counting bacterium / virus 8 antibody bacterium virus bacterium/virus A electrode bacterium/virus B silicon bacterium/virus C bacterium/virus D HeLa cells electrode electrode HeLa cell % Potential vs. Ag/AgCl [V] Electrode without HeLa cell % Potential vs. Ag/AgCl [V] Electrode with HeLa cell. Ota, K. Niitsu, H. Kondo, M. Hori, and K. Nakazato, BIOCA214

9 BioCMO 64x64 potentiometric sensor array with temperature control 9 heater 64x64 sensor array thermometer Power upply(5v) DD address A~A4 Row Address strobe Column Address strobe RA CA address buffer Y decoder 64x64 sensor array multiplexer X decoder address buffer out Analog output 6mm temperature [ ] set measure time [sec] 2mm Au electrode

10 BioCMO 32x32 potentiometric/amperometric/impedimetric sensor array with temperature control 1 Power DD upply(5v) address buffer Y decoder 32x32 sensor array Reset RT address counter clock ADC ADC Parallel-in serial out shift register Fine:1bit Coarse:1bit Total: 2bit D-9 PCLK HYNC VYNC 6mm 2mm Au electrode

11 BioCMO 32x32 potentiometric/amperometric/impedimetric sensor l sensor array with electrophoretic control & temperature control 11 Power upply(5v) DD address A~A4 Row Address strobe Column Address strobe RA input voltage Vin CA Y decoder address buffer 32x32 array sensor analog voltage memory multiplexer voltage buffer X decoder address buffer out Analog output 6mm supply voltage Au electrode 2mm sensor Au electrode