Quality Consistency Confidence

Transcription

1 Gene Link

2 Quaity Consistency Confidence For over a decade Gene Link has been providing researchers with the finest critica genetic research toos. Consistenty maintaining our reputation and responsibiity to suppy quaity products, we present our entire ine with unrivaed confidence. Our products and services are supported and ensured by our commitment to premium quaity and our constant efforts to introduce innovative products and cuttingedge technoogy to the research community wordwide. Gene Link fosters customer satisfaction and oyaty by stressing persona reationships with our customers. Our dedicated and experty trained customer service and technica support staff are motivated to serve our customers in any way possibe. Routiney assisting our customers in the design of their experiments and other technica inquiries, we stay committed and connected to our customers who have entrusted us with suppying the toos they need in furthering their exciting and groundbreaking research. Gene Link has deveoped, and wi continue to preserve, a reputation for Quaity, Consistency, and Confidence. A eading suppier of premium custom oigonuceotides, researchers turn to Gene Link for demanding appications and consistent resuts. Gene Link services incude genotyping, sequencing and gene construction; as we as a wide variety of other moecuar bioogy products such as, sirna, fuorescent probes, genetic toos and reagents, and non-radioactive gene detection systems for human genetic disorders. GOLD STANDARD Speciaizing in Long Oigos up to 250 mer Gene Link

3 Gene Detection Systems Gene Link is the eader in tripe repeat disorder genotyping using non-radioactive based methods. We have more than a decade of expertise and have deveoped facie non-radioactive detection methods for safe, sensitive and reiabe genotyping of human genetic disorders. Take a ook at the simpe agarose and poyacryamide ge based systems, the chemiuminescent Southern bot detection methods and the fuorescent systems for genotyping of tripe repeat disorders. The moecuar basis of genetic disorders is as varied as cinica genetics itsef. The moecuar etioogy of disorders may be fundamentay straightforward, such as in Sicke ce disease, as compared to a whoe new cass of diseases where anticipation is invoved with apparenty increasing disease severity in successive generations. Addressing the etioogy and moecuar diagnosis of the more compex disorders that invove anticipation (i.e., Fragie X syndrome, Myotonic dystrophy, and Huntington s disease) is often chaenging. This product profie of Gene Link s current gene detection product ine spotights non-radioactive detection products as we as conventiona radioactive based methods for genotyping the chaenging tripe repeat Genemer PCR Anaysis Agarose/ Poyacryamide Ge 1 Day Gene Detection System Fow Sheet PCRProber PCR Anaysis Poyacryamide Ge Bot Membrane Chemiuminescent 1 1 / 2 Day Resuts, Anaysis and Interpretation disorders as we as singe base mutations and various pathogens. Contro DNA fragments with varying number of tripe repeats, singe base mutations and pathogen contro DNA fragments are aso avaiabe. GScan PCR Anaysis Genetic Anaysis 1 Day GeneProber Day 1 Restriction Digestion Agarose Ge Eectrophoresis Day 2 Day 3 Agarose Ge Bot Membrane Chemiuminescent Southern Bot Anaysis Ceary distinguishabe two aees (one in maes for X chromosome) Two aees not ceary interpretabe Resuts, Anaysis and Interpretation 1 Gene Link

4 Genemer Genemer are optimized primer pairs for ampification of the gene fragment of interest, particuary those spanning a mutation. A wide range is avaiabe from singe base pair mutation sites, the compex tripe repeat disorders and various pathogens. Genemer kits are compete, easy-to-use systems for reiabe genotyping of arge repeats in certain tripe repeat disorder. Gene Link has deveoped a wide array of specific ampification primers and optimized conditions for gene fragments and specificay for the tripe repeat disorders. For exampe, the Huntington Genemer kit is capabe of routiney ampifying greater than 150 CAG repeats. Other detection systems avaiabe are for singe base mutations and various pathogens. The Genemer product contains 10 nmos of the primer pair that is sufficient for 400 reguar 50 µ ampifications. The Genemer Kit contains reagents as we as contro DNA sufficient for 100 ampifications. Genemer PCR Anaysis Agarose/Poyacryamide Ge 1 Day The Gene Link Advantage n No More Hazardous Radioactive Exposure n No More Decayed Probes n Safe, Sensitive and Reiabe Genotyping n Optimized Kits for Reproducibe Resuts n Tripe Repeat Disorder Genotyping n Wide Array of Detection Methods n Chemiuminescent Detection n Fuorescent Based Genetic Anaysis n Contro DNA Standards for Varying Tripe Repeat Lengths n Wide Seection of Disease Genotyping n Knowedgeabe Technica Support Friedreich s ataxia GAA repeat genotyping was performed using FRDA Genemer (Cataog No ) and various FRDA Genemer Contro DNAs (Cataog No XX). Optimized reagents from the FRDA Genemer Kit were used to ampify ong repeats. Lanes 1 & 6 are moecuar weight markers; anes 2 5 are 64, 102, 110 and 125 GAA repeat ampification products. Resuts, Anaysis and Interpretation Ceary distinguishabe two aees (one in maes for X chromosome) Two aees not ceary interpretabe Proceed to GeneProber Detection Method GOLD STANDARD Genemer Product Size Cataog No. Fragie X, Huntington Disease, Myotonic Dystrophy, 1 Kit 40-20XX-11 Friedreich s Ataxia, Kennedy Disease, Sicke Ce, Rh, Sry, and other Genemer Kits avaiabe HIV, HCV, HBV, MTB and MTB Genemer Kit 1 Kit 60-20XX-11 Fragie X, Huntington Disease, Myotonic Dystrophy, 10 nmos 40-20XX-10 Friedreich s Ataxia, Kennedy Disease, Sicke Ce, Rh, Sry, and other Genemer primer pairs avaiabe First Round Genotyping The Genemer and GScan products are for initia screening. A sampes yieding disease causing tripe repeats shoud be confirmed by Southern bot anaysis using the GeneProber product ine to confirm genotype. Visit for compete isting of Gene Detection Systems product ine. Gene Link 2

5 GeneProber A specific gene fragment probe for Southern bot based hybridization of genomic DNA. The GeneProber is avaiabe unabeed for radioactive based methods and abeed with digoxigenin for chemiuminescent detection. One tube is suppied containing 500 ng of the yophiized unabeed GeneProber probe. The quantity suppied is sufficient for at east 5 random prime abeing reactions using 100 ng for each reaction. Gene Link recommends using 25 ng of probe for each abeing reaction. The digoxigenin abeed probe suppied is sufficient for five 20 x 20 bots. The GeneProber product ine is based on the chemiuminescent Southern bot detection method. These probes are speciay deveoped to detect the tripe repeat ampifications. Fragie X, Huntington disease, Myotonic dystrophy, Friedreich s ataxia and Kennedy disease GeneProber unabeed and digoxigenin abeed are avaiabe. Gene Link s non-radioactive detection systems for genotyping of tripe repeat disorders are rapid, reiabe and as sensitive as the 32 P abeed southern bots. No more decayed probes and radioactive exposure. A detaied manua accompanies the product giving a step by step protoco, resuts and interpretation guideines. CpG isand CGG repeats EcoRI *BssHII (2463) *BssHII *BssHII *EagI (2436) *NruI (2339) GeneProber Day 1 Restriction Digestion Agarose Ge Eectrophoresis Day 2 Day 3 Fragie X Southern Bot Anaysis 2755 bp 2781 bp 2878 bp Agarose Ge Bot Membrane Chemiuminescent Southern Bot Anaysis Resuts, Anaysis and Interpretation EcoRI Fragie X CGG repeats genotyping resuts using Gene Link s GeneProber products. Fragie X GeneProber GLFXDig1 Digoxigenin-abeed Probe (Cataog No ) and GeneProber GLFX1 Unabeed Probe (Cataog No ) were used to probe human bood genomic DNA digested with Eco RI and Eag I. Left bot was hybridized with digoxigenin abeed probe and processed for chemiuminescent detection. The bot on the right was hybridized with 32 P abeed GLFX1 and processed for radioactive Southern bot detection. Both bots were exposed overnight to X-ray fims. Norma fragment sizes for femaes are between 2.8 kb and 5.2 kb, and a singe fragment of 2.8 kb in maes. The bot shows sampes with CGG repeats in the affected range. GeneProber Product Size Cataog No. Fragie X, Huntington Disease, Myotonic Dystrophy, 500 ng 40-20XX-40 Friedreich s Ataxia and Kennedy Disease GeneProber unabeed probe Fragie X, Huntington Disease, Myotonic Dystrophy, 110 µ 40-20XX-41 Friedreich s Ataxia and Kennedy Disease GeneProber digoxigenin abeed probe Visit for compete isting of Gene Detection Systems product ine. 3 Gene Link

6 PCRProber Gene Detection Kits Gene Detection Systems PCRProber akaine phosphatase abeed probe is for ampification and non-radioactive detection of a trinuceotide repeat region ampified PCR product. The PCRProber Kit comprises of a primer pair for PCR ampification foowed by ge bot and chemiuminescent detection using the akaine phosphatase oigonuceotide probe. Quantity suppied is sufficient for 100 ampifications. Gene Link s PCRProber Kit is based on PCR ampification foowed by Southern bot chemiuminescent detection using an akaine phosphatase abeed oigonuceotide probe. This kit is a safe and sensitive aternate to radioactive-based detection methods. The ampified products are resoved on a sequencing poyacryamide ge, and then botted and processed for chemiuminescent detection. The PCRProber Kit is simpe and robust for routine tripe repeat detection of greater than 100 repeats of a tripe repeat disorders isted, except Fragie X. The CGG repeat in Fragie X can be detected up to ~50 repeats. It is strongy recommended that the genotyping be foowed up by using GeneProber Southern bot detection methods when two aees are not ceary discernabe. PCRProber PCR Anaysis Bot Membrane Poyacryamide Ge Chemiuminescent Fragie X CGG repeats genotyping resuts using Gene Link s PCRProber. Various human genomic DNA sampes were ampified using the reagents suppied in the PCRProber Kit (Cataog No ) and processed for chemiuminescent detection foowing the protoco in the manua provided. Fragie X CGG repeat genotyping as an initia screening using the PCRProber is rapid as it ampifies CGG repeat sizes up to ~50 repeats. Lane 1 in the bot shows a femae sampe containing 29 and ~60 CGG repeats respectivey. 1 1 / 2 Day Resuts, Anaysis and Interpretation Ceary distinguishabe two aees (one in maes for X chromosome) Two aees not ceary interpretabe Proceed to GeneProber Detection Method PCRProber Kits Product Size Cataog No. Fragie X, Huntington Disease, Myotonic Dystrophy, 1 kit 40-20XX-32 Friedreich s Ataxia and Kennedy Disease PCRProber Kits avaiabe. Fragie X, Huntington Disease, Myotonic Dystrophy, 12 µ 40-20XX-31 Friedreich s Ataxia and Kennedy Disease PCRProber avaiabe. Visit for compete isting of Gene Detection Systems product ine. Gene Link 4

7 GScan Gene Detection Kits GScan Kits contain optimized PCR ampification reagents and a wide seection of fuorescentabeed primers for genotyping after PCR using fuorescent genetic anayzer instrument(s). Kit incudes sufficient reagents for 100 detections. Genotyping using this kit requires use of the appropriate fuorescent genetic anayzer instrument(s) and software capabe of detection of fuorescenty abeed fragments of varying engths. These kits have been optimized for an ABI310 genetic anayzer. Gene Link s GScan gene detection kits are safe, convenient and sensitive, and afford automated compiation of data. The kits contain optimized PCR ampification reagents and a wide array of fuorescent-abeed primers for genotyping after PCR using fuorescent genetic anayzer instrument(s). Incuded in these kits are ready-to-run contro sampes of various repeats of the tripe repeat disorder. These contro sampes are for caibration with the moecuar weight markers for accurate size determination of the ampified fragments. The GScan Kits are simpe and robust for routine tripe-repeat detection of greater than 100 repeats of a tripe repeat disorders isted, except Fragie X. The CGG repeat in Fragie X can be detected up to ~50 repeats. It is strongy recommended that the genotyping be foowed up by using Southern bot detection methods when two aees are not ceary discernabe. GScan PCR Anaysis Genetic Anaysis 1 Day Resuts, Anaysis and Interpretation Ceary distinguishabe two aees (one in maes for X chromosome) Two aees not ceary interpretabe Proceed to GeneProber Detection Method Above:Myotonic Dystophy CTG genotyping Right: Huntington Disease CAG genotyping GScan Gene Detection Kits Product Size Cataog No. Fragie X, Huntington Disease, Myotonic Dystrophy, Friedreich s Ataxia and Kennedy 1 Kit 40-20XX-15 Disease GScan Kits avaiabe. Visit for compete isting of Gene Detection Systems product ine. GScan Dye Labeed Markers Product Cataog No. Size GScan Marker Tamra abeed 50 bp bp µ GScan Marker Tamra abeed 50 bp bp µ GScan Marker Hex abeed 50 bp bp µ GScan Marker Hex abeed 50 bp bp µ *A oading of 0.5 µ is suggested. 5 Gene Link

8 Genemer Contro DNA Gene Detection Systems Genemer Contro DNA are coned fragments of a particuar gene for use with gene or mutation specific Genemer and GScan products. These contro DNAs are idea genotyping tempates for optimizing and performing contro ampification with unknown DNA. One tube is suppied containing 500 ng of yophiized DNA segment of the specified fragment spanning the mutation region. The quantity suppied is sufficient for 1000 reguar 50 µ PCR reactions. The contro DNAs were deveoped to compement Gene Link s gene detection system product ine. The contro DNAs for a singe base mutation are hepfu to optimize the protoco and in setting up positive contro standards. The contro DNA series for various tripe repeat disorders were generated by ampification. These serve as contros for the ampification of various tripe repeats and to run as positive contros. The size of the tripe repeats has been determined by sequencing and ge eectrophoresis. The stabiity of size repeats upon coning and ampification has not been determined. Thus, the size shoud be considered approximate and there is no caim for each fragment to contain the exact number of tripe repeats. These contro DNAs are sod with the expressed condition that these not be used for exact tripe repeat size determination of DNA of unknown genotype. The contro DNA shoud be used for determining the performance of specific Genemer, GScan and PCRProber Gene Link products. Lane M is moecuar weight markers. Lanes 1-5 represent PCR products from DM genomic cones that contain 12, 45, 93, 129, and 182 CTG repeats respectivey. Sicke Ce Genemer contro DNA for Hb-A, Hb-S and Hb-C. Huntington's Disease Genemer contro DNA of various CAG repeats shown here used for GScan anaysis. Genemer Contro DNA Kits Product Size Cataog No. Fragie X, Huntington Disease, Myotonic Dystrophy, Friedreich s Ataxia and Kennedy Disease contro DNA 500 ng 40-20XX-XX avaiabe with varying number of tripe repeats. Contro DNA aso avaiabe for singe base mutation disorders. HIV, HBV, HCV, MTB, and WNV pathogen contro Genemer DNA. 500 ng 60-20XX-06 Pease visit for other Genemer Contro DNA not isted here. Gene Link 6

9 RNA Interference RNAi Exporer RNAi Exporer from Gene Link is a series of products and services to aid researchers in exporing RNA interference. Onine search and design toos for sirna and shrna Guaranteed RNAi Exporer kit for gene knockout Gene Link. Resuts You Can Rey On. Gene Detection Systems Non-radioactive gene detection systems for the rapid and reiabe detection of various gene segments. Genemer Genemer products are avaiabe for Fragie X, Huntington s disease, Myotonic dystrophy, Freidreich s ataxia, Sicke ce, Sry, X and Y, etc. Pease see our website for a compete isting. GeneProber Rapid, non-radioactive, southern-based genotyping of tripe-repeat disorders such as Fragie X, Huntington s disease, Myotonic dystrophy and Friedreich s ataxia. GScan Convenient gene detection systems for automated fuorescent genetic anayzer instruments. GScan systems avaiabe for Fragie X, Huntington s disease, Myotonic dystrophy and Friedreich s ataxia. Oigo Synthesis Gene Link Oigo Synthesis Division is not an oigo factory. Order Gene Link oigos for demanding appications and consistent resuts. A Ge picture is suppied with each oigo. Long oigo synthesis, up to 250 mer; a modifications avaiabe Moecuar Beacons, TaqMan Probes, fuorescent dye abeed oigos and probes Easy onine ordering Buy one, get one at no charge on your first order Custom Sequencing Premium DNA sequencing resuts for a eves of service. ConeIDSeq For rapid cone and PCR product identification. Accuracy greater than 97%. DSSeq Pubishing quaity doube strand sequencing. Accuracy greater than 99%. Bac/Pac/P1Seq Read ength of 300 bases guaranteed. Sequence accuracy is greater than 95%.

10 A WEALTH OF INFORMATION Gene Link Web Resource This cataog is a brief profie of our product ine. We encourage you to visit our website for detaied information. There is a weath of information aways avaiabe at your desktop that is just a cick away. Customer Service & Technica Support Quick Question Each page of our website contains a page sensitive Quick Question feature at the bottom. Type any question and your e-mai address, and receive a quick repy usuay within 30 minutes. Ask a quick question, get a quick answer.

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