PowerPlex ESX & ESI Fast Systems

Transcription

1 PowerPlex ESX & ESI Fast Systems

2 PowerPlex ESX & ESI Fast Systems The PowerPlex ESX and ESI Fast Systems meet recommendations of the European Network of Forensic Science Institutes (ENFSI) for DNA profile sharing across Europe. Using rapid cycling technology, amplification can be done in less than 5 minutes. The systems are compatible with the Applied Biosystems 313, 313xl and 35 Genetic Analyzers and ABI PRISM 31, 31-Avant and 31 Genetic Analyzers. Rapid thermal cycling (<5 minutes) saves hours per run Multiple kit configurations facilitate confirmation of results from poor-quality samples One kit for casework and database samples simplifies laboratory quality control and inventory management Amplification of common loci allows simplified data sharing across borders Superior inhibitor tolerance and amplification of loci as mini-strs ensure a high success rate with challenging casework samples Based on the current PowerPlex ESX and ESI Systems, the systems allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, TH1, vwa, D2S441, D1S1248, D22S145, D1S1656, D12S391, D2S1338, D16S539, D19S433, SE33 (optional) and Amelogenin. A Size (bases) A* D3S1358 THO1 D21S11 D18S51 D1S1248 D1S1656 D2S1338 D16S539 D22S145 vwa D8S1179 FGA D2S441 D12S391 D19S433 SE33 B Size (bases) A* D3S1358 D19S433 D2S1338 D22S145 D16S539 D18S51 D1S1656 D1S1248 D2S441 THO1 vwa D21S11 D12S391 *Amelogenin 8469MA Figure 1. Panel A. PowerPlex ESX Fast System amplifies the five ENFSI-recommended loci as mini-strs. Offered with or without SE33. Panel B. PowerPlex ESI Fast System amplifies six of the seven European Standard Set (ESS) loci as mini-strs. Offered with or without SE33. A = Amelogenin.

3 Achieve Superior Results in One Third the Time Recognizing that laboratories are being asked to do more in less time, we ve designed the PowerPlex ESI and ESX Fast Systems to enable amplification to be completed in less than 5 minutes. The shortened cycling times free up laboratory personnel to work on more value-added activities such as DNA analysis and interpretation of results. The PowerPlex ESX 17 Fast System The PowerPlex ESI 17 Fast System 4, Amel D3S1358 TH1 D21S11 D18S , Amel D3S1358 D19S433 D2S1338 D22S , 2, 4, D1S1248 D1S1656 D2S1338 D16S , D16S539 D18S51 D1S1656 D1S1248 D2S , 2, 4, D22S145 vwa D8S1179 FGA , TH1 vwa D21S11 D12S , 2, D2S441 D12S391 D19S433 SE33 4, , , 2, WEN ILS 5 ESS WEN ILS 5 ESS MA MA Figure 2. 28M Control DNA (.5ng) was amplified with the PowerPlex ESX 17 Fast System for 3 cycles. Amplification products were separated using an Applied Biosystems 313 Genetic Analyzer (3kV, 5-second injection). Figure 3. 28M Control DNA (.5ng) was amplified with the PowerPlex ESI 17 Fast System for 3 cycles. Amplification products were separated using an Applied Biosystems 313 Genetic Analyzer (3kV, 5-second injection). Fifty-minute amplification time enables faster turnaround times.

4 PowerPlex ESX & ESI Fast Systems Obtain More Complete Profiles Even With Challenging Samples Low template and degraded DNA samples are often seen with casework samples and can contribute to poor results. The primers in the PowerPlex ESI and ESX Fast Systems amplify loci as mini-strs to generate complete profiles from the most challenging samples. Additionally, the robust buffer minimizes the need to re-amplify problematic samples. The PowerPlex ESI and ESX Fast Systems reliably produce complete STR profiles from as little as 1pg of human DNA, and the majority of alleles can be called with as little as 3pg of DNA. Gain Superior Sensitivity DNA Amount PowerPlex ESI 17 Fast System PowerPlex ESI 17 Pro System PowerPlex ESX 17 Fast System PowerPlex ESX 17 System 5pg 1% 1% 1% 1% 1pg 1% 1% 1% 1% 6pg 1% 99% 1% 1% 3pg 85% 76% 98% 9% Figure 4. The PowerPlex ESI and ESX Fast Systems demonstrate superior sensitivity, reliably generating full profiles with as little as 1pg of DNA. Varying amounts of DNA amounts were amplified in 25µl reactions with PowerPlex ESI and ESX 17 Fast Systems and PowerPlex ESI 17 Pro and ESX Systems (n = 3). Amplified products were separated using an Applied Biosystems 313 Genetic Analyzer (3kV, 5-second injection). The percentage of total alleles called is indicated for each DNA amount. Produce complete profiles under the most difficult conditions.

5 Implement Direct Amplification from Numerous Sample Types The PowerPlex ESI and ESX Fast Systems allow direct amplification from FTA card punches as well as pretreated nonfta cards and commonly used swabs to streamline workflows and expand sample throughput. Laboratories can achieve significant time savings and heightened efficiency due to the minimal number of steps involved in sample preparation. FTA Punches NonFTA Punches Swabs Punch FTA card into plate Punch card into plate; treat with PunchSolution Reagent Incubate swab with SwabSolution Reagent Add PCR amplification mix Add PCR amplification mix to dried punch Add 2µl of extract into PCR amplification mix 11513MA <5-Minute cycling time for all applications <5-Minute cycling time for all applications FTA Punches The PowerPlex ESI and ESX Fast Systems facilitate direct amplification of DNA from unwashed FTA card punches, improving laboratories workflows and saving more than two hours per plate. Blood Sample on an FTA Punch, PowerPlex ESI 17 Fast System Buccal Sample on an FTA Punch, PowerPlex ESI 17 Fast System Amel D3S1358 D19S433 D2S1338 D22S145 Amel D3S1358 D19S433 D2S1338 D22S145 4, ,9 2, 2,6 1,3 4, D16S539 D18S51 D1S1656 D1S1248 D2S D16S539 D18S51 D1S1656 D1S1248 D2S ,9 2, 2,6 1,3 4, TH1 vwa D21S11 D12S TH1 vwa D21S11 D12S ,9 2, 2,6 1,3 4, ,9 2, 2, MA 1, MA Figure 5. Direct amplification of one 1.2mm punch from a blood sample on an FTA card using the recommended protocol. Amplified products were separated using an Applied Biosystems 313xl Genetic Analyzer (3kV, 5-second injection). Figure 6. Direct amplification of two 1.2mm punches from a buccal sample on an FTA card using the recommended protocol. Amplified products were separated using an Applied Biosystems 313xl Genetic Analyzer (3kV, 5-second injection).

6 PowerPlex ESX & ESI Fast Systems Swabs Working in combination with the SwabSolution Kit, the PowerPlex ESI and ESX Fast Systems allow you to reproducibly obtain high-quality STR profiles from swab samples. Minimizing the number of steps involved in sample preparation enhances throughput capabilities, leading to faster turnaround times and shortened time to results. Buccal Sample on Cotton Swab, PowerPlex ESI 17 Fast System Amel D3S1358 D19S433 D2S1338 D22S D16S539 D18S51 D1S1656 D1S1248 D2S TH1 vwa D21S11 D12S MA Figure 7. A buccal swab was pretreated with SwabSolution Reagent. Following incubation, 2µl of extract was added to the PCR amplification mix and amplified using the recommended protocol. Amplified products were separated using an Applied Biosystems 313xl Genetic Analyzer (3kV, 5-second injection). NonFTA Punches The PowerPlex ESI and ESX Fast Systems work in conjunction with the PunchSolution Kit to provide robust profiles from nonfta card punches, including Bode Buccal DNA Collector devices and S&S 93 paper. Buccal Sample on Bode Buccal DNA Collector Device, PowerPlex ESI 17 Fast System 5,4 3,6 1,8 Amel D3S1358 D19S433 D2S1338 D22S D16S539 D18S51 D1S1656 D1S1248 D2S ,4 3,6 1,8 TH1 vwa D21S11 D12S ,4 3,6 1,8 D8S1179 FGA SE ,4 3,6 1,8 1152MA Figure 8. A single 1.2mm punch from a buccal sample collected using a Bode Buccal DNA Collector device was pretreated with PunchSolution reagent, then amplified using the recommended protocol. Amplified products were separated using an Applied Biosystems 313xl Genetic Analyzer (3kV, 5-second injection).

7 More Options. More Results. Less Time. Easier Exchange of National and International Data More shared loci result in higher power of discrimination and a better hit rate More Choices Multiple kit configurations Options with and without SE33 Confidence in Your Results Ability to test with multiple primer pairs Confirmatory testing for null alleles Time Savings 5-minute cycling time Reduced need for repeat testing Simple processing of a variety of sample types More Complete Profiles Robust buffer for superior inhibitor tolerance Mini-STR configurations for a higher success rate with challenging samples Ordering Information PRODUCT SIZE CAT.# RELATED PRODUCTS SIZE CAT.# PowerPlex ESX 17 Fast System PowerPlex ESI 17 Fast System 4 reactions DC171 1 reactions DC reactions DC172 1 reactions DC1721 SwabSolution Kit 1 preps DC8271 PunchSolution Kit 1 preps DC9271 5X AmpSolution Reagent 5µl DM1231 PowerPlex ESX 17 Fast System and PowerPlex ESI 17 Fast System Bundle PowerPlex ESX 16 Fast System 4 reactions DC173 1 reactions DC reactions DC161 1 reactions DC1611 PowerPlex 5C Matrix Standards, 31 PowerPlex 5C Matrix Standard WEN Internal Lane Standard 5 ESS 5µl (each dye) DG564 5 preps DG485 2µl DG511 PowerPlex ESI 16 Fast System PowerPlex ESX 16 Fast System and PowerPlex ESI 16 Fast System Bundle 4 reactions DC162 1 reactions DC reactions DC163 1 reactions DC1631 The PowerPlex ESX and ESI Fast Systems are designed specifically for use with the ABI PRISM 31 and 31-Avant Genetic Analyzers and Applied Biosystems 313, 313xl, 35 and 35xL Genetic Analyzers.

8 Maxwell, Plexor and PowerPlex are registered trademarks of Promega Corporation. Identity Automation, AmpSolution, Differex, DNA IQ, PunchSolution, Slicprep, STR Normalization Manager and SwabSolution are trademarks of Promega Corporation. ABI PRISM, Applied Biosystems and GeneMapper are registered trademarks of Applied Biosystems. FTA is a registered trademark of Flinders Technologies, Pty, Ltd., and is licensed to Whatman. Bode Buccal DNA Collector is a trademark of the Bode Technology Group, Inc. Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information. PROMEGA CORPORATION 28 WOODS HOLLOW ROAD MADISON, WI USA TELEPHONE PROMEGA CORPORATION ALL RIGHTS RESERVED PRICES AND SPECIFICATIONS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE PRINTED IN USA 5/ PART #BR25