Mycotoxins SOLUTIONS BY. Sample Preparation and Analysis

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1 Immunoffinity Columns Automted Smple Preprtion ELISA Rpid Tests Photochemicl Derivtiztion Post-column Derivtiztion with PICKERING Vcuum Mnifold Selected HPLC Column Support Mycotoxins Smple Preprtion nd Anlysis Cultivtion nd storge of food nd niml feed my contribute to the spred of moulds, which produce mycotoxins - toxic secondry metbolites. Their consumption cn led to serious helth dmge in both humns nd nimls. LCTech supports you with wide rnge of relible products t competitive prices. In ddition you cn rely on outstnding customer support. SOLUTIONS BY

2 About LCTech LCTech GmbH is bsed in Dorfen, Germny. Since 1998, we hve been developing nd supplying products nd methods for the preprtion nd nlysis of food, niml feed nd environmentl smples. Our product rnge includes both semi nd fully utomted smple preprtion systems s well s consumbles utilised in the nlysis of contminnts nd residues. LCTech is represented worldwide through welldeveloped distributor network nd lso cts s the exclusive distributor for PICKERING Lbortories in Europe, Afric nd the Arb countries. Worldwide, mny customers including governmentl nd contrct lbortories focusing on food nd feed nlysis, nd lso those bsed in the phrmceuticl industry or reserch - re eqully pprecitive of both our modern nd efficient products nd the responsive nd competent customer support. Customer stisfction is our top priority Michel Bumnn, Mnging Director!! Visit us on Get further nd permnently updted product informtion on our website. Mycotoxins: Smple Preprtion nd Anlysis

3 Mycotoxins Smple Preprtion nd Anlysis Precise, Simple nd Fst with LCTech Products Clen-up of the extrcted smple Immunoffinity columns, pges 2-3 Automted smple preprtion (AcceCLEAN TM ), pges 4-5 Vcuum mnifold (EluVc TM ), pge 10 Chromtogrphy Selected HPLC column, pge 11 Derivtiztion Photochemicl derivtiztion (UVE TM ), pge 8 Post-column derivtiztion with PICKERING, pge 9 Detection ELISA rpid test, pges 6-7 Our support for you, pge 12 Selected references relting to our products, pge mycotoxins@lctech.de 1

4 Immunoffinity Columns Fst nd Efficient Smple Clen-Up Proven nd tested The LCTech immunoffinity columns re suitble for the clen-up of food nd feed smples redy for subsequent nlysis. With high mximum loding cpcities the mesuring rnge is wide. The ntibodies show high specificity ginst the respective mycotoxin. The chromtogrphic results re excellent without interfering signls nd chieve very high recovery rtes even in the most difficult smples. The immunoffinity columns re successfully employed for the most diverse mtrices in ccredited lbortories worldwide. These columns performed well in interntionl interlbortory trils. Advntges t glnce High loding cpcities Gurnteed high recovery rtes Long storge life up to 24 months Storge t room temperture Advntgeous 3 ml column formt vilble: Lrger dimeter results in incresed tolernce for mtrix contmintions Detiled protocols for processing lrge vriety of smples re vilble Competent, free of chrge ppliction support Pcks of 25 pieces or of bulk pcks with 500 pieces vilble For lrge smple volumes corresponding reservoirs re vilble Certified qulity LCTech meets the high demnds of Europen nd interntionl legl requirements concerning mycotoxin nlysis nd controls every single production step. A detiled qulity certificte is included in ech pck. One of our customers writes: Food nd Environment Reserch Agency (FERA) Deprtment of Nturl Toxins United Kingdom "LCTech immunoffinity columns offer high qulity smple clen up combined with gret vlue. The UK Food nd Environmentl Agency, (FERA, formerly CSL) evluted the columns throughout 2008 nd received strong technicl support from LCTech during the process, llowing us to tilor stndrd protocols to our methods esily nd quickly. The products hve enbled us to offer fr more cost effective service to our customers." 2 Mycotoxins: Smple Preprtion nd Anlysis

5 LCTech immunoffinity columns t glnce Immunoffinity column Mycotoxin Size, Pcs (Product N ) Recoveries Shelf life nd temperture Loding cpcity, ng/column AflCLEAN Select Afltoxins B1, B2, G1, G2 1 ml, 25 pcs (12062) 1 ml, 100 pcs (12323) 1 ml, 500 pcs (12063) 3 ml, 25 pcs (12058) 3 ml, 500 pcs (12059) B1 > 90 B2 > 80 G1 > 90 G2 > 60 9 months cooled t 4 C 200 (fltoxin B1) OtCLEAN TM Ochrtoxin A 1 ml, 25 pcs (12425) 1 ml, 100 pcs (12426) 1 ml, 500 pcs (12427) > 90 % 24 months room temperture ml, 25 pcs (10515) 3 ml, 500 pcs (11535) Afl-OtCLEAN TM Afltoxins B1, B2, 3 ml, 25 pcs (11022) 3 ml, 500 pcs (11771) > 90 % (fltoxin B1) > 80 % (ochrtoxin A) 12 months room temperture 150 (fltoxin B1) 200 (ochrtoxin A) G1, G2 nd ochrtoxin A Combintion column - fst clen-up in tndem Afltoxins nd ochrtoxin A often occur together nd re therefore jointly nlysed. The combintion column Afl-OtCLEAN TM mkes your work much esier by clening the extrct within one processing step. Smple reservoirs for lrge smple volumes, mde of Durn glss, reusble nd dishwsher proof 2 pcs / pck (10896)!! Are you considering switching to LCTech columns nd would like to try them? Plese simply sk for our support! x Mykotoxine: Probenvorbereitung - mycotoxins@lctech.de und Anlytik 3

6 AcceCLEAN TM Prlleliztion - Invluble Time Sving Process Design 30 columns in one run... 4 Mycotoxins: Smple Preprtion nd Anlysis

7 Automted Smple Preprtion... nd Three t One Blow Fully utomted extrct clen-up The LCTech smple preprtion system AcceCLEAN TM uses utomted rw extrct processing with immunoffinity columns. Consequently, smples re clened up fster nd more conveniently nd re redy for subsequent nlysis.!! Flexibility counts! The system AcceCLEAN TM is lso suitble for vrious solid phse extrctions (SPE). Three needles = threefold throughput AcceCLEAN TM hndles up to three smples simultneously nd up to 30 in one run. This system enbles speedy smple preprtion t n ffordble price. AcceCLEAN utomtes time-consuming procedures. Brod rnge of pplictions Over time your pplictions my chnge. However, AcceCLEAN TM stys flexible nd cn be dpted t nytime. Advntges t glnce Substncilly incresed smple throughput Excellent recovery rtes Intuitive opertion without PC High qulity components for high precision Robust, sfe design Pre-set methods for LCTech immunoffinity columns Three needles in prllel triple smple throughput. The rcks re esily chrged. The columns remin closed whilst on the system positive pressure ensures constnt flow rtes. Ordering informtion Product N AcceCLEAN TM Automted system for immunoffinity nd SPE columns Rcks, ccessories nd instlltion on request x Mykotoxine: Probenvorbereitung - mycotoxins@lctech.de und Anlytik 5

8 ELISA Rpid Tests Ech consignment is checked in-house for its functionlity nd reproducibility. The result is documented with qulity certificte by LCTech. Simple, Fst nd Cost-Efficient Screening for Afltoxin B1 nd Ochrtoxin A in Food nd Feed ELISA rpid test Mycotoxin Mesuring rnge Processing time Shelf life nd temperture Product N AflREAD TM Afltoxin B1 AflREAD fst Afltoxin B1 in penuts nd mize NEW ppb 130 minutes 12 months cooled t 4 C ppb < 45 minutes 12 months cooled t 4 C OtREAD TM Ochrtoxin A 2-20 ppb, for beer ppb, for wine ppb 130 minutes 12 months cooled t 4 C OtREAD fst Ochrtoxin A in penuts nd mize NEW 2-20 ppb < 25 minutes 12 months cooled t 4 C Photometer with 450 nm filter, lso mobile use on-site Incredibly simple hndling The rpid tests re redy-to-use: They contin 96-well ELISA plte; they cn lso be used individully in 8-well strips. All required regents nd stndrd solutions re included. An extensive mnul together with set of descriptive brief instructions led even inexperienced lb stff sfely through the uncomplicted ppliction. Even for difficult mtrices, such s coffee or red wine, clen-up of the smple extrct is not necessry (e.g. with SPE or IAC). Advntges t glnce Complete, low-cost rpid tests The sme extrct my be used for ll rpid tests Only two extrction methods for solid smples Best reproducibility nd repetbility through stndrdistion Esy-to-understnd softwre t no chrge Brod mesuring rnge Stisfctory recovery rte: % Competent customer support Relible results The direct comprison between mesurements with AflREAD TM or OtREAD TM to those with immunoffinity columns nd HPLC/FLD shows good correltion (see tbles). The rpid tests deliver relible results. Even with difficult mtrices, such s rost coffee, the mesurements with ELISA brely differ from those with HPLC: Smple ELISA [ppb] HPLC [ppb] Rost coffee Direct comprison between mesurements with OtREAD TM nd those with immunoffinity columns nd HPLC/FLD; certified reference mteril with ppb (BD475). Are you currently introducing mycotoxin nlysis into your lbortory? We gldly offer our support! 6 Mycotoxins: Smple Preprtion nd Anlysis

9 Smple AflREAD TM [ppb] HPLC [ppb] For the detection of fltoxin B1 Exceptionlly fst test for penuts nd mize Rice Penut butter < LOQ* < LOQ* Brzil nut < LOQ* Pst < LOQ* Direct comprison between mesurements with the rpid test AflREAD TM nd those with IAC columns nd HPLC/FLD * LOQ = limit of quntifiction Smple OtREAD TM [ppb] HPLC [ppb] For the detection of Ochrtoxin A Exceptionlly fst test for penuts nd mize Rice < LOQ* Pst < LOQ* Animl feed < LOQ* Direct comprison between mesurements with the rpid test OtREAD TM nd those with IAC columns nd HPLC/FLD * LOQ = limit of quntifiction Helpful mnuls An extensive mnul nd set of cler brief instructions led through the dectection with the ELISA rpid test. Fst results For immedite nd esy reding of your results, we recommend our flexible, robust nd trnsportble LCTech photometer. x Mykotoxine: Probenvorbereitung - mycotoxins@lctech.de und Anlytik 7

10 Photochemicl Derivtiztion Perfect Afltoxin Anlysis t n Attrctive Price!! No sensible lterntive. Simpler, more robust nd fster thn ny other comprble method for this kind of nlysis. Cnny nd proven UVE TM is suitble for the photochemicl post-column derivtiztion of fltoxins. UVE TM is smll, hndy device - 15 cm wide, 9 cm high, 27 cm deep. The result is distinctly enhnced signl for the importnt fltoxins G1 nd B1. The method is ccepted by the AOAC, is successfully employed in inter-lbortory trils nd in ccredited lbortories worldwide. The lterntive to Cobr-cell Photochemicl derivtiztion hs dvntges over electrochemicl bromintion. This ws shown by Muscrell et l. (see pg. 13). The big plus point in comprison: The wter present is used s regent. Neither iodine nor HNO 3 /KBr re being used. Advntges t glnce No regents neeeded Cn be used together with ny HPLC Components re designed to sustin opertion of over severl thousnd hours. Simple confirmtion nlysis fter switch-off of the rector Multiple sfety fetures No time-consuming rinsing fter usge Inexpensive nd low mintennce Esiest instlltion: Connect with HPLC nd detector, switch on, redy. HPLC UVE FLD Ordering informtion Product N UVE M Photochemicl rector for the nlysis of fltoxins, 254 nm lmp (230 VAC) ( VAC) m V Without UVE TM m V With UVE TM : distinctly enhnced signl B G G 2 B G 2 B 2 The derivtiztion of the fltoxins B1 nd G1 to stble fluorescent derivtives is performed with UV-light in specil rector loop mde from completely inert mteril G 1 B Subsequent detection is conducted t 365 / 460 nm (FLD). m in ,0 1,3 2,5 3,8 5,0 6,3 7,5 8,8 1 0,0 m in ,0 1,3 2,5 3,8 5,0 6,3 7,5 8,8 1 0,0 8 Mycotoxins: Smple Preprtion nd Anlysis

11 Post-Column Derivtiztion with PICKERING For the Perfect Mycotoxin Anlysis with HPLC The professionl system: PINNACLE PCX The PINNACLE PCX by PICKERING Lbortories is professionl system for post-column derivtiztion within mycotoxin nlysis. The PINNACLE PCX for professionl post-column derivtiztion Substnces tht re not or only brley visible for the detector rect fter seprtion with regent. Thereby the detection sensitivity is significntly incresed. This method is suitble for the detection of fltoxins, fumonisins, ochrtoxin A nd deoxynivlenol. Wht hppens during derivtiztion Afltoxins B1 nd G1 re trnsformed to stble fluorescent derivtives using n queous iodine solution. An lkline shift of the ph increses the fluorescence of ochrtoxin A significntly. Fumonisins re trnsformed into fluorescent isoindole derivtives by complex rection trgeting their mine functions. Formldehyde is initilly split off from deoxynivlenol, which is subsequently trnsformed to fluorescent lutidine derivtive. Brod rnge of pplictions The PINNACLE PCX cn be used with ll common HPLC brnds. It cn be esily dpted to every required ppliction - by simple nd fst exchnge of the rector nd selection of the storble method vi softwre. Detiled protocols re vilble for the individul mycotoxins. Advntges t glnce Significntly improved detection limit through derivtiztion Enhnced sensitivity Pulse-free bse line ttributble to injection pump Simple hndling: cn be supervised by less experienced lb stff Low mintennce: no delicte wering prts Automted system rinsing Chemiclly inert Mtrix insensitivity Ordering informtion Product N PINNACLE PCX Professionl system for post-column derivtiztion Afltoxins, 1.4 ml rector, single pump Fumonsins, 0.5 ml rector, single pump Ochrtoxin A, 0.15 ml rector, single pump Trichothecene, spec. rector, dul pump x Mykotoxine: Probenvorbereitung - mycotoxins@lctech.de und Anlytik 9

12 Vcuum Mnifold EluVc TM Prllelized Clen-Up vi Immunoffinity Columns More smples in less time The vcuum mnifold EluVc TM llows the simultneous clen-up of up to 20 smples under vcuum in preprtion for mycotoxin nlysis. Thus, smple throughput in the lbortory cn be significntly incresed with little effort. Flexible nd dptble Effortless nd quick, the EluVc TM cn be dpted to vrious pplictions for solid phse extrction in environmentl, food nd feed nlysis. For these purposes smple rcks for different vils re vilble from LCTech. EluVc TM, Vcuum mnifold by LCTech Another detil provides flexibility: For wste collection either centrl 600 ml beker or pipe connected to n externl reservoir re used. As result of this, the EluVc TM is lso suitble for lrger smple volumes, e.g. milk or wter smples. Comfortble hndling System opertion of the EluVc TM is very esy. Smple rcks for different vils mke the sytem dptble to mny chllenges. One big plus point: By simply turning the lid you cn switch from wste to collect position without disssembling the device. Advntges t glnce Vluble time nd cost sving due to enhnced smple throughput Robust nd suitble for dily lbortory routine work Chemiclly resistnt For lrge smple volumes corresponding reservoirs re vilble For wste collection: beker or - s shown here - centrl pipe tht cn be connected to Woulff s bottle. Ordering informtion Product N EluVc M Vcuum mnifold set contining system, smple rck nd collection rck for 4 ml vils Further sets nd rcks on request 10 Mycotoxins: Smple Preprtion nd Anlysis

13 Selected HPLC Column For the Anlysis of Afltoxins B1, B2, G1 nd G2 Reduced opertion time A gurnteed theoreticl plte number of t lest 120,000 ensures good bse-line seprtion with short running times - fltoxin B1 lredy elutes in less thn 8 minutes. Afltoxin B1 in less thn 8 minutes The LCTech HPLC column significntly reduces your nlysis time. You cn mke svings in two wys: On the one hnd the columns themselves re inexpensive nd on the other shorter processing times incur less consumption of eluents. The column cn lso be used for the nlysis of ochrtoxin A. In order to protect the HPLC column from clogging in the long term, it is highly recommended to use gurd column to cpture ny remining mtrix compounds prior to the nlyticl column. We will be plesed to send you suitble isocrtic methods on request. Ordering informtion Product N HPLC column for the nlysis of fltoxins nd ochrtoxin A, RP C Gurd column, RP C18, 3 pieces / pck Gurd column holder 10750!! All-inclusive! Synchronised nlysis of fltoxins with LCTech immunoffinity-columns, HPLC columns, photochemicl derivtiztion nd ll together with excellent customer support. x Mykotoxine: Probenvorbereitung - mycotoxins@lctech.de und Anlytik 11

14 Customer Support From Smple Preprtion to Anlysis Support in every spect One of our customers wrote: We run our own lbortory for ll pplictions here in Dorfen nd mintin close contct with our customers using our products. Both spects contributed to our extensive experience built over the yers in the field of mycotoxin nlysis. With this in mind, we will be plesed to support ll your enquiries nd/or requests. "Thnk you so much for your speedy ssistnce s lwys nd the gret interest you tke in our concerns. Your help is invluble to us nd it feels quite refreshing nd mkes welcome chnge to hving found compny redy to support our pplictions with such enthusism. Thnk you gin! Regrdless whether you recently introduced this nlysis in your lbortory or whether you re currently deling with prticulrly difficult nd unusul mtrix we will lwys do our best to help. Support mterils Mny different mtrices, be it mrnthus or cinnmon, hve been tested in our lbortory. Amrnthus or cinnmon The knowledge won from these tests hs been compiled in recommendtions explining how to del best with the clen-ups of the respective mtrices. Extensive user mnuls for our product rnge cn be supplied on request free of chrge. Would you like to find out more bout postcolumn derivtiztion within mycotoxin nlysis? Detiled informtion is vilble to you t ny time. Extensive user mnuls Your direct contct to LCTech It will tke only one phone cll or e-mil to hve your question directly in the lbortory. Do you need to sk questions or would you like to mke suggestions concerning our products? We will be there for you t ny time. We gurntee our customers to nswer to n enquiry within 24 hours. Dr. Uwe Aulwurm, Hed of Applictions Tel. +49 (0) mycotoxins@lctech.de 12 Mycotoxins: Smple Preprtion nd Anlysis

15 Chosen References for Our Products AOAC Officil Method , Afltoxins in corn, rw penuts nd penut butter FAPAS Proficiency Test 0490 Report, Afltoxin nlysis in pistchio, Juni - August 2006 FAPAS Proficiency Test Report, Afltoxins B & G in mize, October - November 2009 Mrgos, C.M., 2009, Mycotoxin Reserch, Photorection of indole-contining mycotoxins to fluorescent products. 25(2): Muscrell, M. et l., Food Additives nd Contminnts, Vol. 26, No. 10, October 2009, , Vlidtion of confirmtory nlyticl method for the determintion of fltoxins B1, B2, G1 nd G2 in foods nd feed mterils by HPLC with on-line photochemicl derivtiztion nd fluorescence detection Ofitserov, M. et l., J AOAC Int. 2009, Jn-Feb; 92 (1), 15-25, Multiresidue mycotoxin nlysis in corn grin by column high-performnce liquid chromtogrphy with postcolumn photochemicl nd chemicl derivtiztion: Single lbortory vlidtion Ppdopoulou-Bouroui A., Strok J., Anklm E., J., AOAC Int. Vol. 85, No. 2, 2002, , Comprison of two post-column derivtiztion systems, ultrviolet irrdition nd electrochemicl determintion, for the liquid chromtogrphic determintion of fltoxins in food Reiter, E.V. t l., 2009, Afltoxins in plm kernel cke from Indonesi Applicbility of ELISA in contrst to HPLC st Mycotoxin Workshop; June 15 17, 2009; Münster, Germny Reiter E.V. et l., A limited survey of fltoxins in rice products mrketed in Vienn, 31st Mycotoxin Workshop; June 15 17, 2009; Münster, Germny Trucksess, M.W. et l., J AOAC Int Jul-Aug; 90 (4) , Use of mycotoxin columns for determintion of fltoxins nd ochrtoxin A in ginseng nd ginger Vukovic, G. L. et l., Arch. Biol. Sci, Belgrde, 61 (4), , 2009, Comprison of two smple preprtion procedures for HPLC determintion of Ochrtoxin A x - mycotoxins@lctech.de 13

16 LCTech GmbH And distributor nerby Bhnweg Dorfen, Germny Tel. +49 (0)8081 / Fx +49 (0)8081 / info@lctech.de Visit us on We reserve the right for technicl chnges. LCTech ssumes no libility for the bove nd the illustrted ppliction exmples. LCTech GmbH, Germny P/N 12278, s of October 2010 SOLUTIONS BY