Product Datasheet. VEGF Antibody (VG1) NB Unit Size: 0.1 mg

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1 Product Datasheet VEGF Antibody (VG1) NB Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 35 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 9/17/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB VEGF Antibody (VG1) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer 0.1 mg 1 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal VG1 0.02% Sodium Azide IgG1 Kappa Protein G purified PBS Page 1 of 7 v.20.1 Updated 9/17/2018 Product Description Host Mouse Gene ID 7422 Gene Symbol Species Specificity/Sensitivity Immunogen Product Application Details Applications VEGFA Human, Mouse, Rat, Canine This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF Recombinant VEGF 189 protein. Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, CyTOF-ready Recommended Dilutions Western Blot 1-2 ug/ml, Flow Cytometry, ELISA, Immunohistochemistry 1:20-1:100, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:100, Immunohistochemistry-Frozen 1:20-1:100, CyTOF-ready Application Notes This VEGF antibody is useful for Immunohistochemistry (frozen and paraffinembedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, ph 8.0 must be performed prior to the immunostaining.

3 Images Flow Cytometry: VEGF Antibody (VG1) [NB ] - An intracellular stain was performed on U-87 cells with VEGF [VG1] Antibody NB APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Page 2 of 7 v.20.1 Updated 9/17/2018 Western Blot: VEGF Antibody (VG1) [NB ] - HUVEC and 293T Cells tested at 1:1000. Image provided by verified customer review. Immunohistochemistry-Paraffin: VEGF Antibody (VG1) [NB ] - IHC analysis of a formalin-fixed paraffin embedded human angiosarcoma tissue section using VEGF antibody (clone VG1). The endothelial cells of the blood vessels and most of the cancer cells showed strong positivity for VEGF protein. Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG1) [NB ] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-vegf (VG1) [NB ] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

4 Flow Cytometry: VEGF Antibody (VG1) [NB ] - Analysis of PerCP conjugate of NB An intracellular stain was performed on HUVEC cells with VEGF (VG1) antibody NB PCP (blue) and a matched isotype control NBP PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s Page 3 of 7 v.20.1 Updated 9/17/2018 Western Blot: VEGF Antibody (VG1) [NB ] - Western blot analysis of VEGF in human kidney protein using NB Flow Cytometry: VEGF Antibody (VG1) [NB ] - An intracellular stain was performed on U937 cells with VEGF [VG1] Antibody NB APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG1) [NB ] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with VEGF (VG1) NB at a 1:200 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.

5 Flow Cytometry: VEGF Antibody (VG1) [NB ] - An intracellular stain was performed on U-937 cells with VEGF Antibody (VG1)NB F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to FITC. Page 4 of 7 v.20.1 Updated 9/17/2018 Flow Cytometry: VEGF Antibody (VG1) [NB ] - An intracellular stain was performed on U-937 cells with VEGF Antibody (VG1) NB and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/ml for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).

6 Publications Chen Y, Gao S, Yan Y, Qian J. Aerosolized deferoxamine administration in mouse model of bronchopulmonary dysplasia improve pulmonary development. Am J Transl Res Jan 15 [PMID: ] (WB, Mouse) Xu J, Sun Y, Wu T et al. Enhancement of bone regeneration with the accordion technique via HIF-1alpha/VEGF activation in a rat distraction osteogenesis model J Tissue Eng Regen Med 2017 Aug 01 [PMID: ] (Rat) Du C, Mendelson AA, Guan Q et al. Hyperbranched polyglycerol is superior to glucose for long-term preservation of peritoneal membrane in a rat model of chronic peritoneal dialysis. J Transl Med. Dec :00AM [PMID: ] (IHC, Rat) Lin YC, Chen RY, Chen SW et al. Immunohistochemical studies and fluorodeoxyglucose uptake on positron emission tomography in pharyngeal cancer for predicting radiotherapy-based treatment outcomes. Clin Otolaryngol. Oct :00AM [PMID: ] (IHC) Chen H, Jia P, Kang H et al. Upregulating Hif-1alpha by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound. Adv Healthc Mater 2016 Apr 20 [PMID: ] Su JC, Mar AC, Wu SH et al. Disrupting VEGF-A paracrine and autocrine loops by targeting SHP-1 suppresses triple negative breast cancer metastasis Sci Rep 2016 Jul 01 [PMID: ] (IHC) Chen SW, Lin YC, Chen RY et al. Immunohistochemical overexpression of hypoxia-induced factor 1a associated with slow reduction in (18)fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer. Eur. J. Nucl. Med. Mol. Imaging Jun :00AM [PMID: ] (IHC) Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals Oct [PMID: ] (WB, Mouse) Ram M, Singh V, Kumawat S et al. Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats. Eur J Pharmacol 2015 Oct 5 [PMID: ] (Rat) Ram M, Singh V, Kumawat S et al. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int. Immunopharmacol Jan 01 [PMID: ] (WB, Rat) Details: VEGF antibody (clone VG1) was used at 1.5 ug/ml concentration for the analysis of granulation/healing tissues of control and bilirubin-treated diabetic rats (Fig. 4). Srinivas C, Ramaiah MJ, Lavanya A et al. Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated micrornas and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer. PLoS ONE Nov 10 [PMID: ] (WB, Human) Details: VEGF antibody (clone VG1) IMG was used for WB analysis of lysates from MCF-7 and MDA-MB-231 cells that were transfected with microrna-15, 16, 17 and 221 followed by treatments with Cisplatin, Etoposide or Etoposide analogue, Quinazolino-4?-amidopodophyllotoxin /C-10. Shao J, Zhang Y, Yang T et al. HIF-1alpha disturbs osteoblasts and osteoclasts coupling in bone remodeling by upregulating OPG expression In Vitro Cell. Dev. Biol. Anim Apr 10 [PMID: ] (WB, Mouse) More publications at Page 5 of 7 v.20.1 Updated 9/17/2018

7 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB HAF007 NB720-B NBP mg Human Kidney Whole Tissue Lysate (Adult Whole Normal) Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preadsorbed) Mouse IgG1 Kappa Light Chain Isotype Control (P ) Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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