Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Transcription

1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department University Hospital of North Durham Durham County Durham DH1 5TW Contact: Mrs Sharon Campbell Tel: +44 (0) Website: Testing performed at the above address only Routine samples: Aspirates on swabs, catheter tips and intrauterine contraceptive devices Fluids Genital samples Pus Samples from sterile sites Semen Tissue Bone Surgical Implants on swabs Blood and fluids from sterile sites Sputum Nasal secretions Bronchial lavage Nasopharyngeal aspirates DETAIL OF ACCREDITATION diagnosis Screening for Methicillin-Resistant Staphylococcus aureus (MRSA) LP/PA/MB/A1.010 (Eyes) LP/PA/MB/A1.004 (ENT) LP/PA/MB/A1.010 (Wounds) LP/PA/MB/A1.010 (Genitals) LP/PA/MP/A9.001 (s) LP/PA/MB/A1.002 Manual culture using chromogenic selective solid agar media using: LP/PA/MB/A1.007 Liquid media culture using automated continuous monitoring system: BD BACTEC Fx Blood Culture analyser using manufacturer s instruction and: LP/PA/MB/PE6.003 LP/PA/MB/A1.003 (Sputum/BAL) LP/PA/MB/A6.028 (RSV) Assessment Manager: GS2 Page 1 of 8

2 Fluids: Ascitic fluid Joint fluids Cerebrospinal fluid (CSF) Pus Drain fluids Peritoneal fluid Continual Peritoneal Ambulatory Dialysis Wound Bile Pleural Intra-ocular fluid Corneal scrapes on swabs Cultures isolated in-house from all samples listed above Cultures isolated in-house from all samples listed above Screening for Carbapenemaseproducing Enterobacteriaceae (CPE) Isolation, identification and antimicrobial susceptibility of bacterial pathogens: Campylobacter spp. Clostridium difficile Escherichia coli O157 Salmonella sp. Shigella spp. Vibrio spp. Yersinia spp. Identification of microorganisms of Identification of Staphylococcal isolates LP/PA/MB/A1.002 (fluids) LP/PA/MB/A1.007 (Cerebrospinal fluid) LP/PA/MB/A1.013 Manual culture using chromogenic selective solid agar media using: LP/PA/MB/A1.015 LP/PA/MB/A4.001 Conventional identification methods including biochemical, latex agglutinations: LP/PA/MB/A6.002 (API) LP/PA/MB/A (General organism identification procedures) Bio-rad Pastorex Staph Plus LP/PA/MB/A6.024 Assessment Manager: GS2 Page 2 of 8

3 Cultures isolated in-house from all samples listed above Cultures isolated in house from the above Cultures isolated in house from the above Bacterial cultures isolated in-house from all samples listed above Fluids Tissues Pus Cerebrospinal fluid Ascitic fluid Continual Peritoneal Ambulatory Dialysis (CAPD) Genital swabs Cerebrospinal fluid Continual Peritoneal Ambulatory Dialysis Ascitic fluids Cerebrospinal fluid Continual Peritoneal Ambulatory Dialysis Ascitic fluids Serological identification of groups A, B, C, D, F and G of the Lancefield groups of Streptococci grown on agar plates Detection of Salmonella polyvalent 'O' (PSO) and Salmonella polyvalent 'H' (PSH); Shigella species Identification of E. coli O157 Antimicrobial susceptibility testing of clinically significant bacteria Detection, identification and semiquantification of: White blood cells Bacterial cells Yeast cells and fungal hyphae Clue cells White blood cell type differential Quantification of white and red blood cells. Pro-Lab Streptococcal Grouping kit. LP/PA/MB/A6.025 Oxoid agglutination sera Oxoid E.coli O157 Latex Test LP/PA/MB/A6.014 Manual (EUCAST) by disc diffusion and gradient MIC using SOPs: LP/PA/MB/A (AST) LP/PA/MB/A (AST) LP/PA/MB/A6.028 (MIC) Direct examination using staining techniques and light microscopy. LP/PA/MB/A7.006 (Gram stain) LP/PA/MB/A7.003 (Indian Ink) Direct examination using staining techniques and light microscopy. LP/PA/MB/A7.006 (Gram stain) LP/PA/MB/A7.005 (Methylene blue) Direct manual microscopy using light microscopy: LP/PA/MB/A1.002 (ASF) LP/PA/MB/A1.007 LP/PA/MB/A1.011 (CAPD) Assessment Manager: GS2 Page 3 of 8

4 Sputum Bronchial Lavage Fluid/pus Cerebrospinal fluid on swabs Quantification of white blood cells and epithelial cells. Detection and characterisation of urinary casts Screening for Acid Fast Bacilli Automated microscopy: 1Q200 Sprint urine analyser using LP/ND/MB/PE6.058 Direct examination using light microscopy and SOP: LP/ND/MB/PE6.058 (contingency) Automated microscopy: 1Q200 Sprint urine analyser using LP/ND/MB/PE6.058 Direct examination using light microscopy and SOP: LP/ND/MB/PE6.058 (contingency) Fluorescent microscopy using an auramine phenol stain and confirmation using a Ziehl Neelsen staining and light microscopy LP/PA/MB/A5.001 General AFB procedures LP/PA/MB/A5.007 Auramine phenol LP/PA/MB/A5.009 Ziehl Neelson Detection of ova, cysts and parasites Concentration using midi-parasep concentrator and examination of deposit by light microscopy following manufacturer's instructions and SOP: LP/PA/MB/A4.002 Pus/fluids Detection of ova, cysts and parasites Direct preparations observed using light microscopy following SOP: LP/PA/MB/A4.002 Assessment Manager: GS2 Page 4 of 8

5 Perianal Swab Detection of E.vermicularis ova and larvae Concentration using centrifugation and examination of deposit using light microscopy following SOP: LP/PA/MB/A4.002 Detection of Schistosoma spp Concentration using centrifugation and examination of deposit using light microscopy following SOP: LP/PA/MB/A4.002 on swabs (genital) Female genital tract swabs Skin Nail hair Nasopharyngeal aspirates, washes and swabs Detection of Cryptosporida spp and Cyclospora spp Detection of Trichomonas vaginalis Microscopic diagnosis of bacterial vaginosis Isolation and identification of clinically significant dermatophyte fungi Detection of respiratory syncytial virus (RSV) Fluorescent microscopy using an auramine phenol stain and confirmation using a Ziehl Neelsen staining and light microscopy following SOP: LP/PA/MB/A4.002 Culture using liquid broth media and examination with light microscopy following SOP: LP/PA/MB/A1.005 Gram stained microscopy examination using: LP/PA/MB/A1.010 Direct microscopy of KOH digested samples and culture on selective solid agar media using SOP: LP/PA/MB/A8.001 Lateral flow Immunoassay (Meridian Bioscience) using manufacturer s instructions and: LP/PA/MB/A6.028 Assessment Manager: GS2 Page 5 of 8

6 Detection of toxigenic Clostridium difficile GDH antigen Detection of toxigenic Clostridium difficile Toxin A&B Gene detection for Toxin A&B of toxigenic Clostridium difficile Detection of Rota Virus and Adenovirus Lateral flow chromatograph for detection of GDH antigen (Pro-lab) using manufacturer's instructions and SOP: LP/PA/MB/A2.027 Enzyme linked fluorescent immunoassay (ELFA) for detection of toxin A&B using a mini-vidas (Biomerieux) following SOP: LP/PA/MB/A2.024 Clostridium difficile toxin A & B gene detection: real time PCR using GeneXpert analyser (Cepheid) with SOP: LP/PA/MB/PE6.057 Lateral flow immunochromatograph (Biokit) for Rotavirus and Adenovirus antigens using manufacturer's instructions and SOP LP/PA/MB/A2.023 Detection of Norovirus Lateral flow immunochromatograph (r- Biopharm) for Norovirus antigen using SOP LP/PA/MB/A2.028 Serum Detection Helicobacter pylori Enzyme immunoassay (Meridian Bioscience) for H.pylori antigen using the DS2 analyser following manufacturers' instructions and SOPs: LP/DM/MB/PE6.049 Assessment Manager: GS2 Page 6 of 8

7 on swabs Detection of Chlamydia trachomatis Neisseria gonorrhoeae Herpes simplex virus 1&2 Serological examination activities for the purposes of clinical diagnosis Molecular detection strand displacement amplification on the BD Viper analyser following SOPs: LP/PA/MB/PE.054 (Viper) LP/PA/MB/A2.031 (CT/GC) LP/PA/MB/A2.032 (HSV) Serum Detection of: Enzyme linked fluorescent immunoassay (ELFA) using: LP/PA/MB/A2.025 LP/PA/MB/PE6.046 and manufacturer s instructions for Biomerieux Mini Vidas analyser Hep A IgM Hep A total IgM and IgG Hep B surface Ag Hep B core Ab Hep B surface Ab Hep C Ab Rubella Ab HIVp24 Ag HIV 1 &HIV 2 Ab Measles virus IgG VZV IgG Hep C ag Assessment Manager: GS2 Page 7 of 8

8 Serological examination activities for the purposes of clinical Serum Detection of: Enzyme Immunoassay (Biokit) using: LP/PA/MB/A2.014 LP/PA/MB/PE6.044 LP/PA/MB/A2.004 LP/PA/MB/A2.012 and manufacturer s instructions for Best 2000 Hep B surface Ag Syphilis Ab Serum Determination of Anti-Streptolysin O titre Manual test (Biokit) following manufacturer s instructions and LP/PA/MB/A2.007 END Assessment Manager: GS2 Page 8 of 8