CERTIFICATE OF ANALYSIS

Transcription

1 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67560M Lot #: 10K31608 Description: Phosphoserine (Set of 6 MAbs) Set Includes: Catalog #K67810M Catalog #K67430M Catalog #K67904M Catalog #K67440M Catalog #K67270M Catalog #K67604M Clone BDI810 Clone BDI430 Clone BDI904 Clone BDI440 Clone BDI270 Clone BDI604 Specificity: Clone: Phosphoserine Detection kit combines 25ug of each of the monoclonal anti-phosphoserine antibodies. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. SETSER Host Animal: Mouse Isotype: n/a Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water per vial for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography. Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

2 Catalog #K67560M Page 2 of 2 Applications: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Catalog #K67560M Phosphoserine Detection Phosphoprotein Positive Control was probed with: Lane 1: Catalog #K67810M (1ug/ml) Lane 2: Catalog #K67430M (1ug/ml) Lane 3: Catalog #K67904M (1ug/ml) Lane 4: Catalog #K67440M (1ug/ml) Lane 5: Catalog #K67270M (1ug/ml) Lane 6: Catalog #K67604M (1ug/ml) Storage: Warning: Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY 30 September 2013 Date

3 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67810M Lot #: 10K31608 Description: Specificity: Clone: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI810 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI810 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized. Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

4 Catalog #K67810M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

5 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67430M Lot #: 10K31608 Description: Specificity: Clone: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI430 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI430 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

6 Catalog #K67430M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

7 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67904M Lot #: 10K31608 Description: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Specificity: Clone: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI904 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI904 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

8 Catalog #K67904M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

9 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67440M Lot #: 10K31608 Description: Specificity: Clone: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI440 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI440 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

10 Catalog #K67440M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

11 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67270M Lot #: 10K31608 Description: Specificity: Clone: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI270 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI270 Host Animal: Mouse Isotype: IgG 1, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphoserine conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

12 Catalog #K67270M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored (up to 3 months) at 2-8 C. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

13 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67604M Lot #: 10K31608 Description: Specificity: Clone: MAb to Phosphoserine Monoclonal Antibody to Phosphoserine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. Clone BDI604 recognizes a broad range of serine-phosphorylated proteins in crude cell extracts preferring positively charged amino acids directly neighbored to phosphoserine. Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Reacts with human, mouse, rat and dog. BDI604 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Tissue culture Phosphopeptide conjugated to KLH Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium azide (prior to lyophilization)

14 Catalog #K67604M Page 2 of 2 Applications: Storage: Warning: Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored at 2-8 C for up to 3 months. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY