L-COHR Service Request Catalog

Transcription

1 L-COHR Service Request Catalog MicroCT, Digital Imaging, and Laser Capture Microdissection (LCM) MicroCT scanning/analysis Unassisted microct usage (Prior approval required) Digital imaging and analysis Unassisted imaging and LCM usage (Prior approval required) Time charged in 15 minute intervals. Includes time necessary to prepare and load fixed samples into scanner, actual scan time (determined by scanner and requested resolution), unloading of samples from scanner, and any time required for analysis requested by customer. If samples are not adequately trimmed or prepared, the time required to complete these will be included in the charges. Time charged in 15 minute intervals. Includes time to complete scans without staff assistance. Training by COHR staff and prior approval required. (Training charged at full rate ($32/hour)). Time charged in 15 minute intervals. Includes time to acquire images, analyze and save files as requested by customer. Time charged in 15 minute intervals. Includes use of Olympus BX61 microscope, DP72 camera and CellSens software for brightfield or fluorescent imaging. Training by COHR staff and prior approval required. (Training charged at full rate ($60/hour)). $24/hour $12/hour $60/hour $20/hour Page 1

2 Subcutaneous or IP injection Oral cavity injection Changing of 4NQO water Oral examination and weight measurement Animal Services Includes anesthesia, injection of cells or compound subq or IP and monitoring during recovery. Mice to be supplied by investigator. Includes anesthesia, injection of cells or compound into the oral cavity under a dissecting microscope and monitoring during recovery. Mice to be supplied by investigator. Preparation of 4NQO water and filling water bottle with 4NQO water. Includes anesthesia, oral image acquisition under a dissecting microscope, and weight measurement. $6/injection $10/injection $5/cage $5/mouse Weight measurement Includes weight measurement. $2/mouse Mouse dissection with image acquisition Murine frozen tissues in RNA Later solution Murine tissue array (defined) Includes dissection, collection of tissues as requested by customer, written dissection notes, and image acquisition under a dissecting microscope. Labeled tubes containing appropriate fixative and other reagents must be supplied by investigator. Murine tongue, esophageal cancerous lesions, or control tissues were harvested from the 4NQO cancer model. Customers can use the frozen tissues for RNA or protein analysis. Murine tongue, esophageal cancerous lesions, or control tissues from 4NQO cancer model. The defined array will contain 18 squamous cell carcinoma lesions, 24 precancerous lesions, and 12 control normal tissues. Customers can use the paraffin array sections for H&E staining and IHC staining. $50/hour $100/up to 4 samples $50/array Soft Diet Provide low-weight mice with soft food diet. $2/cage Page 2

3 Histology Services Tissue decalcification Tissue preparation Paraffin/OCT block preparation First cut/block refacing charge Paraffin/OCT section on slide Slide deparaffinization Decalcification of fixed tissue samples in 0.5 M EDTA or other method by special request until complete (may take up to four weeks). Includes solution changes three times per week. There will be an additional charge for the cost of X-ray film (at cost) if a rapid decalcification method is requested. Time charged in 15 minute intervals. Can include tissue fixation, trimming, labeling, washing and tissue orientation as requested by customer. Includes either processing of fixed samples in Leica TP1020 Tissue processor and embedding in paraffin blocks or freezing sample in OCT. Cost for tissue cassettes is included. Includes trimming and facing or refacing of paraffin or frozen block and first slide with sections. Includes subsequent sections of paraffin or OCT block and up to 3 sections/slide (or more if requested). If requested, OCT sections can be placed on cryotape (provided by Core) for the same charge ($2/section). Includes removal of paraffin from up to 10 slides. Slides will be returned to customer in PBS. $7.50/sample $25/hour $8/block $5/block $2/slide $5/up to 10 slides H & E staining Includes deparaffinization of slide, staining with H&E and coverslipping. $1.50/slide Special stain Plastic embedding/sectioning Ground sectioning Includes deparaffinization of slide, staining with method of choice and coverslipping. Includes sample embedding in plastic resin (MMA unless otherwise requested) and 5 slides with up to 2 sections/slide including one slide stained with method of choice. Includes sample embedding in plastic resin (Spurr s unless otherwise requested), cutting, grinding, polishing and one section stained with method of choice. $5/slide $165/sample $165/sample Page 3

4 Immunohistochemistry Immunohistochemistry Includes deparaffinization, staining of tissue array sections or regular paraffin sections with antibodies, color development, counter staining with hematoxylin and coverslipping. Customer must provide primary antibodies; facility will provide secondary antibodies and all other reagents. Up to 3 slides can be stained for $100 with the same primary antibody at the same time. IgG control will be performed on one section (if multiple sections/slide) at no additional charge. If necessary, customer will be charged for preliminary staining required to determine appropriate primary antibody dilution or antigen retrieval method at the same per slide rate. $100/up to 3 slides Pathological analysis/scoring Pathological Scoring and Analysis Includes microscopic examination of slides, written pathological diagnosis, enumeration of inflammatory cells, and scoring for inflammation or tissuespecific staining and representative images. $100/hour Training Unassisted usage (previous training required) Consumables NanoString Analysis Training on use of the NanoString ncounter system. Requires several brief sessions over at least two days to complete. Time should be arranged at least two weeks in advance. Training time includes run of up to 12 samples on system. Includes use of thermal cycler, prep station and digital analyzer to complete scans without staff assistance. Training by COHR staff and prior approval required. Consumables for up to 12 samples. Charged at cost. $100 $75/12 samples $300 Page 4

5 BMG Fluostar OPTIMA plate reader ABI StepOne Plus SearchLight Poster printing General Laboratory Equipment and Services Use of BMG Fluostar OPTIMA plate reader. All reagents must be supplied by user. Use of ABI StepOne Plus real-time PCR system. All reagents must be supplied by user. Use of SearchLight multiplex ELISA plate reader. All reagents must be supplied by user. Printing of scientific poster on high quality glossy paper. Poster must be provided to COHR as Powerpoint or Adobe pdf file (PC format preferred). Max poster size 42 on one side (second side can be larger). Additional charge for posters containing non-white background. No proofreading included. $25/poster Agilent 2100 Bioanalyzer Use of Agilent 2100 Bioanalyzer. All reagents must be supplied by user. Nikon digital intraoral camera General laboratory equipment use Use of Nikon digital intraoral camera. Sign out camera with COHR Staff. Maximum charge $24/day if kept for multiple days. Use of general laboratory equipment including high-speed and ultracentrifuges, microfuges, balances, cell culture hoods, and other equipment in BSB 129. All reagents must be supplied by user. $2/hour Page 5