Dayanand College, Hisar

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1 Dayanand College, Hisar Session Lesson Plan for B.Sc. V Th Semester (Theory) Name of Assistant Professor:Dr.Raj Rani (Deptt.of Biotechnology, Dayanand College, Hisar) Class: B. Sc. 3 rd Year (5 th Sem.) Subject: Paper XII. Plant Biotechnology Subject Lesson Plan: Odd Semester 20 weeks (from July 2018 to November 2018) Date Topic july Plant Tissue Culture: Introduction/Concept, History, Scope and Applications along with major Achievements. Plant Tissue Culture Laboratory: Layout and organization, different work areas 30july-1August Infrastructure/equipments and instruments and other requirements Aseptic Techniques: General sanitation/cleanliness of PTC laboratory and precautions regarding maintenance of aseptic conditions, 06-8 August Washing, drying and sterilization of glassware, sterilization Of media, surface sterilization, aseptic work station August Micropropagation/clonal propagation of elite species (different routes of multiplication-axillary bud ) August Organ culture: Anther & Pollen culture, ovary, ovule, embryo and endosperm culture concept, technique, applications and limitations 27 August - 29 August Embryo rescue. Protoplast culture: Protoplast isolation, viability test, protoplast culture September Somatic hybridization protoplast fusion techniques (chemical and electro-fusion), selection of hybrids September production of symmetric and asymmetric hybrids and cybrids. Practical applications of somatic hybridization and cybridization.

2 17-19 September UNIT - II Production of secondary metabolites in vitro: introduction, technique and utilities September Transgenic Plants: Introduction and applications. Developing insect resistance, bacterial and fungal disease resistance, virus resistance and abiotic stress tolerance in plants. Improving food quality nutritional enhancement of plants (carbohydrates, seed storage proteins and vitamins). 26 September Assignment submission 01-03October Plants as Bioreactors: antibodies, polymers, industrial enzymes. Edible vaccines October 10October Biotransformation (a brief account only). Plant germ plasm conservation and cryopreservation Assesment Test 15-17October 22-24October Genetic Engineering in plants: Introduction, Plant transformation by Agrobacterium tumefaciens and A. rhizogenes. Ti plasmid. Strategies for gene transfer to plant cells. Binary and cointegrate vectors. Gene targeting in plants. Use of plant viruses as vectors (brief account only) October, Direct DNA transfer/physical methods of gene transfer in plants - micro projectile bombardment electroporation, liposome mediated, Calcium phosphate mediated etc November Doubt classes Dr. Vivek Srivastava (Associate Professor) Head, Dept. of Biotechnology Dr.RajRani (Assistant Professor)

3 DAYANAND COLLEGE, Hisar Department of Biotechnology Lesson Plan Semester III (M. Sc. 2) year Paper BT-115 SUBJECT: Plant Biotechnology Sr. No. Date Topics 1 Aug 1to Aug 7, 2018 Plant genetic transformation: Organization of plant genome Nuclear genome, Chloroplast genome and mitochondrial genome. Transposon and T DNA tagging. Chloroplast transformation vector designing, method and advantages Agrobacterium mediated transformation Ti and Ri plasmids, role of virulence gene,mechanism of T-DNA transfer, vectors based on Ti and Ri plasmids cointegrate andbinary vectors, technique and factors affecting Agrobacterium mediated transformation of plants. 2. Aug 8, 2018 Revision work 3 Aug 9to Aug 14, 2018 Direct gene transfer particle bombardment, PEGmediated, electroporation, microinjection and alternative methods. Screenable and selectable markers, molecular characterization of transformants. Marker free methodologies, methods for multiple gene transfer in plants.gene silencing in transgenic plants. 5 Aug 16 to Aug 23, 2018 Strategies for introducing biotic and abiotic stress resistance/tolerance: Viral resistance; Fungal resistance; Insect resistance; Herbicide resistance; Various abiotic stresses (like drought, salinity, temperature and flooding). 6 Aug 24,2018 Problem solves 7 Aug25 to Aug31, 2018 Genetic engineering of plants for molecular farming/pharming: Production of medically related proteins in plants, nutritional enhancement of plants(carbohydrates, seed

4 8 Sep Seminars taken storage proteins, vitamins), manipulation of flower colors and other value addition compounds (like industrial enzymes). Plant cells as biofactories for the production of secondary metabolites: Production of useful secondary metabolites through plant cell cultures. 9 Sep 2 to Sep 9, 2018 Strategies used for high yield of product development and selection of high yielding cell line cultures, optimization of factors affecting yield of plant cells (physical culture conditions, media and other biochemicals), bioreactors and immobilized plant cell culture, biotransformation, permeabilization of cells and removal of secreted products. 10 Sep 10, 2018 Seminars taken 11 Sep11 to Sep 17, 2018 Plant cells as biofactories for the production of secondary metabolites: Production of useful secondary metabolites through plant cell cultures; Strategies used for high yield of product development and selection of high yielding cell line cultures, Optimization of factors affecting yield of plant cells (physical culture conditions, media and other biochemicals), bioreactors and immobilized plant cell culture, biotransformation, permeabilization of cells and removal of secreted products. 12 Sept 18,2018 Assignments given 13 sep 19 to 25Sep, 2018 Intellectual Property Rights, Biosafety and Ethical Issues Intellectual property rights (IPR); 14 Sep 26,2018 Assessment test 15 Sep 27 to 6oct, 2018 Assignments given 16 Oct 8 to 12 Oct,2018 Patents, trade secrets, copyright, trademarks; Plant genetic resources; 17 Oct 13 to 19 Oct,2018 GATT & TRIPPS; Patenting of biological material; Patenting of transgenic organisms and genes;

5 18 Oct 22 to 29 Oct,2018 Plant breeders rights (PBRs) and farmers rights; Concerns about GM crops environmental, biosafety and ethics 19 Oct 30 to 9 Nov,2018 Revision 20 Nov 9 to 15 Nov,2018 Doubt Classes Dr. Vivek Srivastava (Associate Professor) Head, Dept. of Biotechnology Dr.RajRani (Assistant Professor)