Yield testing in the lab. Tom Osborn Director of Molecular Breeding Technology Monsanto Company

Transcription

1 Yield testing in the lab Tom Osborn Director of Molecular Breeding Technology Monsanto Company

2 Legal Notes and Forward Looking Statements Certain statements contained in this presentation are "forward-looking statements," such as statements concerning the company's anticipated financial results, current and future product performance, regulatory approvals, business and financial plans and other non-historical facts. These statements are based on current expectations and currently available information. However, since these statements are based on factors that involve risks and uncertainties, the company's actual performance and results may differ materially from those described or implied by such forward-looking statements. Factors that could cause or contribute to such differences include, among others: continued competition in seeds, traits and agricultural chemicals; the company's exposure to various contingencies, including those related to intellectual property protection, regulatory compliance and the speed with which approvals are received, and public acceptance of biotechnology products; the success of the company's research and development activities; the outcomes of major lawsuits and the previously-announced SEC investigation; developments related to foreign currencies and economies; successful operation of recent acquisitions; fluctuations in commodity prices; compliance with regulations affecting our manufacturing; the accuracy of the company's estimates related to distribution inventory levels; the recent increases in and expected higher levels of indebtedness; the company's ability to obtain payment for the products that it sells; the effect of weather conditions, natural disasters and accidents on the agriculture business or the company's facilities; and other risks and factors detailed in the company's most recent periodic report to the SEC. Undue reliance should not be placed on these forward-looking statements, which are current only as of the date of this presentation. The company disclaims any current intention or obligation to update any forward-looking statements or any of the factors that may affect actual results. B.t. products may not yet be registered in all states. Check with your Monsanto representative for the registration status in your state.insect control technology provided by Vip3A is utilized under license from Syngenta Crop Protection AG.ALWAYS READ AND FOLLOW PESTICIDE LABEL DIRECTIONS. BioDirect, Bollgard, FieldScripts, Ground Breakers, Monsanto and Vine Design, Monsanto imagine and Vine Design and Respect the Refuge and Cotton Design are trademarks of Monsanto Technology LLC. Please be advised that the attached article(s) <>>.is/are approved for use by <>.for distribution to <> in <>..format until <<>>. FieldView is a registered trademark of Precision Planting LLC 2014 Monsanto Company. Commercialization is dependent on multiple factors, including successful conclusion of the regulatory process. The information presented herein is provided for educational purposes only, and is not and shall not be construed as an offer to sell, or a recommendation to use, any unregistered pesticide for any purpose whatsoever. It is a violation of federal law to promote or offer to sell an unregistered pesticide. 2

3 Yield (Mt/Ha) The Application of Science and Technology in Agriculture has Helped Deliver Increased Productivity and Resource-Use Efficiency World Yields of Staple Food Crops Rice Maize Wheat Soybeans U.S. agricultural output, inputs and productivity Output Inputs Productivity Disease and Insect Resistance Mendelian Laws Germplasm Diversity Applications of Statistics Source: FAOSTAT Index, 1948=1 Source: USDA-ERS data product, Agricultural Productivity in the U.S. (2010) Heterosis 3

4 Genomics & Biotechnology Enable Continued Performance Improvement Performance depends on Optimal Genomic Combinations Existing Genetic Variation Yield, Stress Tolerance, etc. Disease Resistance, etc. Marker enabled selection Yield, Drought Tolerance, etc. Tolerance to Herbicides, Insects, etc. Transgenic Genetic Variation 4

5 The Evolution of the Use of Molecular Markers: Increased Capacity and Decreased Cost Enables Large Scale Plant Breeding Applications Key growth drivers Automation Technological advances Partner demand 4 Generations of Robotic Applications 5

6 Automated Seed Chipping Removes the Bottleneck of Hand Sampling Plant Tissue VS. Labor and land intensive Time-consuming Low-throughput Soy Corn Capable of analyzing 10s of millions of samples per year Fewer errors Cotton Melon Wheat Select and plant only desired seeds! 6

7 14 Acres High density fingerprinting and sequencing technologies enable genome-wide genetic analysis at high density and low cost DNA array chips (e.g. Applied Biosystems GeneTitan & Axiom, >50k Infinium ) High density, reliable Provides genomic reference data for parental lines Genotyping By Sequencing as a way to get lots of data on lots of samples Provides flexibility in information content Cost continues to drop Ion Proton GeneTitan Axiom Yield in the Field Yield in the Lab Infinium GeneTitan and Axiom are registered trademarks of Thermo Fisher Scientific Infinium is a registered trademark of Illumina, Inc;. Ion Proton is a registered trademark of Thermo Fisher Scientific 7

8 Genome-Wide Selection transitions yield trials to the lab and accelerates the rate of genetic gain Genotype breeding population Assign haplotype effects for traits from historic marker/trait data A / B D / E A / F H / K F / G A / X C / E K / M B / F A / S Chromosome: haplotype blocks Trait 1 effects Trait 2 effect: Trait n effect: Accelerated genetic gain via cost effective scaling of population size faster breeding cycles Estimate trait value of individuals from their total haplotype effects Select based on estimated trait values without field testing 8

9 Partner interfaces are key to enabling a completely new, high volume workflow Old workflow Plant replicated field trial Measure yield New workflow Predict yield 9

10 Innovating with partners to create fast, scalable and cost effective genome-wide genotyping DNA Extraction Library Creation Template/Chip Preparation (Ion Chef ) Sequencing (Ion Proton ) Juggernaut - Custom robotic extraction 96 well plate Box of 96 tubes Reduced process variation 6x labor reduction Int l friendly consumables 10

11 Innovating with partners to create fast, scalable and cost effective genome-wide genotyping DNA Extraction Library Creation Template/Chip Preparation (Ion Chef ) Sequencing (Ion Proton ) TGCAs. 1.0 Transition: Server to Cluster Data scalability Faster processing 2.0 Steam and Process Sequencing Data in the Cloud Simplifies multiple site deployment Flexibility in meeting peak needs Massive scalability Raw seq processing GBS GWS Ion Chef and Ion Proton are registered trademarks of Thermo Fisher 11

12 Yield testing in the lab Enables dramatic increase in scale of breeding program 12

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14 Acknowledgements ABGene Adept Robotics Agilent Automation Solutions (formerly Velocity11) Beckman Coulter BioMicroLab BioNex Solutions BioTek BMG Labtech Caliper Life Sciences (formerly Zymark) Computype FluidX Hamilton Robotics Illumina Jouan Robotics LabCyte (formerly Deerac) Liconic LGC (formerly Douglas Scientific and Global Array) Monsanto Global Breeding Monsanto Molecular Breeding Technology Monsanto Information Technology Monsanto Vegetable R&D NimbleGen Protedyne RTS Life Sciences Staubli Robotics Tecan Thermo Fisher (formerly Matrix, CRS, and Life Technologies) Thermo Fisher Scientific and its affiliates are not endorsing, recommending, or promoting any use or application of Thermo Fisher Scientific products presented by third parties during this seminar. Information and materials presented or provided by third parties are provided as-is and without warranty of any kind, including regarding intellectual property rights and reported results. Parties presenting images, text and material represent they have the rights to do so. 14