Secondary Antibodies, Conjugates, Isotype Controls, and Control Sera (Non-immune)

Transcription

1 Secondary Antibodies, Conjugates, Isotype Controls, and Control Sera (Non-immune) Secondary Antibodies, Conjugates Secondary Antibodies are produced in Goat, Rabbit, Sheep or other host animals. Hyperimmune sera are affinity purified and linked to an appropriate signal molecule (HRP, biotin etc). Detailed data sheets can be accessed at the website. Antibodies made to whole to IgG or IgG (H+L) are likely to react with IgG, IgA, and IgM. Antibodies made to IgG-Fc or gamma chain or isotype specific chains are more specific to a given isotype and they are further cross-adsorbed with other immunoglobulins to minimize crossreactivity. For example, anti-mouse IgG-Fc or IgG-gamma chains are specific to IgG only with minimal or no reaction with IgM or IgA. HRP= Peroxidase; AP=Alk. Phosphatase; FITC=Fluorescein; PE=Phycoerythrin; Conj=Conjugate; Gt=Goat; Rb=Rabbit; M=Mouse (mono); Dk=Donkey; Cy3 & Cy5 Conjugates are also available (see a detailed listing at the web site). Secondary antibodies and Conjugates ( Alligator Antibody 2-Ab Unlabeled HRP Conj. To: Host IgG (H+L) Gt A Bat IgG (H+L) Gt A Bird Bovine Cat Chicken Deer/Elk (Cervid) Dog (Fox/Coyo te) Dolphin Ferret FITC-Conj. Biotin-Conj. IgG (H+L) Gt IgM Gt UL HRP BTN IgG (H+L) Gt IgG (Fc) Gt IgA Rb UL F IgG1 Sh UL F B IgM Rb IgA Gt UL F BTN IgG (H+L) Gt UL F BTN IgG-Fc Gt UL F BTN IgM Gt UL F BTN IgE Mo UL BTN AP-Conj. IgG/(H+L) Gt IgG/(H+L) Rb IgG (Fc) Rb IgM Gt IgA Gt IgG/(H+L) Ch A BT IgG/(H+L) Rb A BT IgG (H+L) Gt IgG (Fc) Gt IgM Gt IgE Mo IgE Gt IgA Gt A IgG (H+L) Gt A IgG (Fc) Gt IgA Gt IgG (H+L) Gt IgG ( ) Gt IgM Gt Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 1

2 Secondary Antibodies, Conjugates G. Pig Goat Antibody 2-Ab Unlabeled HRP Conj. FITC-Conj. Biotin-Conj. AP-Conj. To: Host IgG (H+L) Gt IgG (Fc) Gt IgM Gt IgA Rb IgG (H+L) Rb IgG(H+L)Ads Dk IgG Rb IgG Mo IgG (Fc) Rb IgM Rb Hamster IgG (H+L) Gt (Syrian) (ads) Hamster IgG (H+L) Gt (Arme.) Horse IgG (H+L) Gt IgG (fc) Gt IgM Gt IgE Gt Human IgA Gt IgE Gt IgE Mo M IGEH11-M IGEH12-M IGEH13-M IgG (H+L) Gt IgG (Fc) Mo UL IgG (Fc) Gt IgG (Mk Ads) Gt UL F BT IgG1 Mo UL FITC BT AP PE IgG(k) Gt MKUL11-HRP IgG2 Mo UL FITC BT AP PE IgG3 Mo UL FITC BT AP PE IgG4 Mo UL FITC BT AP PE IgM Gt Llama IgG (H+L) Gt A Macaw IgG (H+L) Gt A Monkey IgG (H+L) Gt IgG ( ) Gt IgG (Mk Mo M BT AP PE specific) IgM (mu) Gt IgA (alpha) Gt IgE Rb Mouse IgA Rb IgA Gt Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 2

3 Secondary Antibodies, Conjugates Antibody 2-Ab Unlabeled HRP Conj. To: Host IgE Gt FITC-Conj. Biotin-Conj. AP-Conj. IgG ( ) Gt IgG (Ads) Gt IgG+A+M Gt IgG (H+L) Gt IgG(H+L)Ads Gt UL F BTN IgG1 Gt F BT IgG2a Gt F BT IgG2b Gt F BT IgG2c Gt F BT IgG3 Gt F BT IgM Gt Opossum IgG (H+L) Gt A Pig (swine) IgG (H+L) Gt IgG (Fc) Rb IgA Gt IgM Gt Rabbit IgA Gt IgG (Fc) Gt IgG Ch IgG Dk IgG (ads) Dk IgG (ads) Gt IgG (ads) Bo IgG (H+L) Gt IgM Gt Raccoon IgG (H+L) Gt A Rat IgA Gt IgE Gt IgG (H+L) Gt IgG(H+L)Ads Gt IgG+A+M Gt Rat IgG (Fc) Gt IgG (Fc) Rb R IgG1 Gt UL HRP IgG1 Mo UL HRP FITC BTN PE IgG2a Gt UL HRP IgG2b Gt UL HRP IgG2c Gt UL HRP IgM Gt Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 3

4 Secondary Antibodies, Conjugates Antibody 2-Ab Unlabeled HRP Conj. FITC-Conj. To: Host Sheep IgA Rb Biotin-Conj. AP-Conj. IgG (H+L) Rb IgG (H+L) Dk IgG (Fc) Rb IgM Rb Whale IgG (H+L) Gt A Cy2, Cy3. Cy5, Rhodamine, AMCA and Texas Red Conjugates Antibody Ab Cy2 Conj. Cy3-Conj. Cy5-Conj. Rhodamine AMCA-Conj. Texas Red To: Host Human IgG (H+L) Gt Rabbit IgG (H+L) Gt Goat IgG (H+L) Rb Cy Cy Mouse Rat IgG (H+L) Dk IgG (Fc) Rb IgG(H+L) Gt IgG Gt Cy Cy3 IgG (ads) Rb Cy Cy3 IgG (H+L) Gt IgG1 Mo Cy5 G. pig IgG (H+L) Gt IgG (H+L) Gt Chicken IgG(H+L) Gt Hamster IgG(H+L) Gt Bird IgG (H+L) Gt Streptavidin, Biotin etc Antibodies and Conjugates Items Host Unlabeled HRP Conj. FITC-Conj. Biotin-Conj. AP-Conj. Biotin Gt UL FITC Gt UL BTN Streptavidi n Wheat Germ Aggl. (WGA) Wheat Germ Aggl. (WGA) Rhod.-Conj PE-Conj RP WGA15-N Rb WGA11-S Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 4

5 Control Ig s or Isotype Controls and conjugates Ig s are collected and purified from a healthy serum pool of non-immunized animals. These Ig s can be used as negative control in most immunoassays, including ELISA, Western, and IHC. Please see complete listing at the web site listed under Primary Antibodies Category under Control IgG s ( ) B or BTN =Biotin; or HRP= Peroxidase; AP=Alk. Phosphatase; F or FITC=Fluorescein; PE=Phycoerythrin; Conj=Conjugate; PC=R-PE and Cy5 (dual label); Gt=Goat; Rb=Rabbit; M=Mouse (mono); Dk=Donkey; Cy3 & Cy5 Conjugates are also available (see a detailed listing at the web site). * UL=unlabeled unless mentioned otherwise; Ig s Isotype Unlabeled HRP Conj. FITC-Conj. Biotin-Conj. Control Baboon IgG B Bovine Cat Chicken IgA IgG F B IgG Fc UL F B IgG Fab UL F B IgG Fab UL F B IgG G G G1-B IgG G G G2-B IgM B IgG UL F B IgG Fc UL F B IgG Fab UL F B IgG Fab UL F B IgM UL B IgG hp F B IgG Fc UL F B IgY IgM B Chimp IgG Ch Ch-B Dog Donkey IgG F B IgG Fc FC FC FC-F FC-B IgG Fab IgG Fab FAB FAB2 IgM B IgE IgG F B IgM B Duck IgY Ferret G. Pig IgA IgG IgM IgG F B IgG Fc UL F B IgM Cy5-Conj. Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 5

6 Control Ig s or Isotype Controls and conjugates Goat Ig s Isotype Unlabeled HRP Conj. FITC-Conj. Biotin-Conj. Control IgG F B PE IgG-Fc FC Fc Fc-F Fc-B Goose IgY Hamster (Syrian) Hamster (Armenian ) Horse Human Monkey (Rhesus) IgG Fab FAB FAB- IgG Fab FAB FAB2- IgM FAB- F FAB2-F FAB- B FAB2-B FAB2- PE Cy5-Conj FAB2- Cy5 IgG F B PE PC5 IgM IgG AH IgG F B IgG Fc UL F B IgM B IgA (native) IgA1 (myeloma) IgA2 (myeloma) IgG F B IgG Fab FAB FAB FAB- F FAB- B IgG Fab FAB FAB FAB2-F FAB2-B IgG Fc FC Fc Fc-F Fc-B IgG G G1-H G1-F G1-B G1-PE (myeloma) IgG G G2-H G2-F G2-B (myeloma) IgG G G3-H G3-F G3-B (myeloma) IgG G G4-H G4-F G4-B (myeloma) IgD (myeloma) IgD (native) P-25 IgM (native) F B IgM mu chain MU IgM (myeloma) M M-MP MF MB IgM Fc5u FC5M IgM Fab F B IgE (myeloma) IgE (native) P IgG F B IgM B Cyno. IgG B Sq. Monk. IgG SM Mouse IgA F B PE PC5 IgG F B PE PC5 IgG F B PE PC5 Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 6

7 Control Ig s or Isotype Controls and conjugates Ig s Isotype Unlabeled HRP Conj. FITC-Conj. Biotin-Conj. Cy5-Conj. Control IgG2a F B PE PC5 IgG2b F B PE PC5 IgG2c IgG F B PE PC5 IgG Fc FC F B IgG Fab FAB Fab Fab-F Fab-B IgG Fab FAB Fab Fab2-F Fab2-B IgM F B PE PC5 IgE Fab Fab-F Fab-B Ostrich IgY Pig Quail Rabbit Rat Rat Sheep Turkey IgG F B IgG Fc UL F B IgM B IgG IgY IgG F B PE IgG fab FAB FAB FAB- F FAB- B IgG fab FAB FAb FAb2-F FAb2-B FAb2- PE IgG Fc FC Fc Fc-F Fc-B Fc-PE IgM F B IgG F B PE IgG fab IgG fab FAB FAB2 IgG Fc FC FC FC-F FC-B IgG F B PE PC5 IgG2a F B PE PC5 IgG2b F B PE PC5 IgG2c UL F B PE IgM F B PE IgA IgG F B IgG Fc UL F B IgM B IgG IgY Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 7

8 Negative Control Sera (Non-immune) for ELISA, Western, and IHC Please see complete listing at the web site listed under 2 Antibodies & ELISA Reagents Category under Normal/Pre-immune (control) Sera ( ) Baboon Bovine Chicken Cat Donkey Dog Duck Ferret Hamster Guinea Pig Goat Horse Human Catalog# NBBS-1 NBOS-10 NBOS- NCHS-10 NCTS-1 NDKS-1 NDKS- NDOS-1 NDOS-5 NDUS-1 NFCS-1 NGHS-1 NGHS-5 NGPS-5 NGTS-1 NGTS- NHOS- 10 NHOS- NHOSH- 10 NHOSH- NHUS-1 NHUS-50 Product Description Normal Baboon control (nonimmunized) serum, Adult mixed sex, 1 ml (other sizes available) Normal Bovine control serum (nonimmunized) Mixed breed, 10 ml Normal Bovine control serum (nonimmunized) Mixed breed, ml Normal Chicken control serum (nonimmunized), 10 ml (other sizes available) Normal Cat control serum (nonimmunized) Mixed breed, 1 ml (other sizes available) Normal Donkey serum, Adult mixed sex, 1 ml Normal Donkey control (nonimmunized) serum, Adult mixed sex, ml Normal Dog control (non-immunized) serum, Adult mixed sex, 1 ml Normal Dog control (non-immunized) serum, Adult mixed sex, 5 ml Normal Duck control serum (nonimmunized) Mixed breed, 1 ml Normal Ferret control (nonimmunized) serum, Adult mixed sex, 1 ml Normal Hamster (Syrian) control serum (non-immunized) Mixed breed, 1 ml Normal Hamster (Syrian) control serum (non-immunized) Mixed breed, 5 ml Normal Guinea Pig control serum (non-immunized) Mixed breed, 5 ml Normal Goat control serum (nonimmunized), 1 ml Normal Goat control serum (nonimmunized), ml Normal Horse control serum (nonimmunized) Mixed breed, 10 ml Normal Horse control serum (nonimmunized) Mixed breed, ml Normal Horse control serum (nonimmunized) delipidized heat inactivate Mixed breed, 10 ml Normal Horse control serum (nonimmunized) delipidized heat inactivate Mixed breed, ml Normal Human control serum (nonimmunized) Mixed breed, 1 ml Normal Human control serum (nonimmunized), Adult, mixed sexes, 50 ml Monkey & Primates Mouse Pig Pigeon rabbit Rat Sheep Turkey Catalog# NCMS- DG-1 NCMS- DG-1 NMAG-1 NMCB-1 NMCS-1 NMCS- DG-1 NMMS-1 NMRS-1 NSQS-1 NMOS- 10 NPIS-1 NPIS- 100 NPNS-1 NRBS-1 NRBS- NSPF-1 NRTS-5 NSHS-10 NSHS- NTUS-1 Product Description Normal Chimpanzee control serum, (IgG's-depleted), 1 ml (other sizes available) Normal Chimpanzee control serum, (IgG's-depleted), 1 ml (other sizes available) Normal Monkey African Green (Chlorocebus) control (nonimmunized) serum, Male and Female Available, 1 ml Normal Monkey Capuchin (Cebus) control (non-immunized) serum, Male and Female, 0.5 ml Normal Monkey (Cynomolgus) control (non-immunized) serum, Adult mixed sex, 1 ml Normal Monkey (Cynomolgus) control serum (IgG's-depleted), 1 ml Normal Monkey Marmoset (Callithrix jacchus) control (non-immunized) serum, Male and Female, 0.5 ml Normal Monkey (Rhesus) control (non-immunized) serum, Adult mixed sex, 1 ml Normal Squirrel monkey control (nonimmunized) serum, Adult mixed sex, 1 ml Normal Mouse control serum (nonimmunized) Swiss Webster, 10 ml (other sizes available) Normal Pig (swine/porcine) control serum (non-immunized) Mixed breed, 1 ml Normal Pig (swine/porcine) control serum (non-immunized) Mixed breed, 100 ml Normal Pigeon control serum (nonimmunized) Mixed breed, 1 ml Normal rabbit control serum (nonimmunized) New Zealand White, 1 ml Normal rabbit control serum (nonimmunized) New Zealand White, ml Normal rabbit control serum (nonimmunized, Disease free, SPF rabbits), 1 ml (other sizes available) Normal Rat control serum (nonimmunized) Sprague-Dawley, 5 ml (other sizes available) Normal Sheep control serum (nonimmunized) Mixed breed, 10 ml Normal Sheep control serum (nonimmunized) Mixed breed, ml Normal Turkey control serum (nonimmunized) Mixed breed, 1 ml Alpha Diagnostic Intl Inc., service@4adi.com; (800) ; Web Site: Page 8