SUBSTRATE & EQUIPMENT

Transcription

1 Creil Lab SUNCARE TESTING SUBSTRATE & EQUIPMENT TRAINING & COACHING In Vitro sunscreen testing solutions

2 KEY FIGURES >50 Sunscreen product tested per month SCREEN - CLAIM - CONTROL 2 PMMA substrate models HD6 Molded & SB6 Sandblasted 2 Appliance models HD-SPREADMASTER & HD-THERMASTER 3 Trainning modules 2 Laboratories worldwide >40 Network countries DIGITAL EDITION OF OUR OFFERS Discover our website and discover multimedia content and exclusive articles! & 2

3 WELCOME HELIOSCREEN Dominique LUTZ CEO Scientist Manager Since 1999, the HelioScreen laboratory is the extension of a personal conviction of the merits of In Vitro techniques for the evaluation of solar products - on the condition of perfectly mastering the many technical and even political obstacles to achieve. This conviction was then little shared, even criticized. But what surprises, excitement and satisfaction firstly personal and then within a team, we brought each step of the mastery of our know-how. Our course has never been changed, our convictions have never been shaken, we are never scattered on the altar of profit and imperative development of the company. Our roots are deep, they now feed our expertise and our ability to be and remain pioneers and leaders... AGENDA & Asia Laboratories / 4 QUALITY RESEARCH KNOWLEDGE SUNCARE TESTING SUBSTRATE & EQUIPMENT TRAINING & COACHING Total traceability Involved in innovation Performing In Vitro test since 1999 Sun protection evaluation Essential elements Share our knowledge / 5 / 6 / 7 / 8 / 9 / 10 3

4 TESTING LABORATORIES Since 1999, HelioScreen has been a laboratory dedicated to In Vitro evaluation of solar products. We provide an environment allowing the sun protection assessment of sunscreens in total reliability. HelioScreen, the pionner in In Vitro sunscreen testing HelioScreen forms one of the leader independent groups of institutes for research, development, testing and analysis in the world. For more than 15 years, we have provided state-of-the-art scientific expertise, innovation and nothing less than perfection for the industry as well as trade and consumer organizations in the field of suncare testing. Worldwide leading Worldwide available HelioScreen Asia Co., Ltd. was born out of the joint venture between HelioScreen France and Chemico Inter Corporation Co., Ltd. in The laboratoy is based in Bangkok and serves all ASEAN and Asia countries. With a wide range of tests covering all needs and also Thai, Asia, European and International regulations, HelioScreen Asia Co., Ltd. assists you in full compliance with the latest standards and official methods. Specific methods are also available to assist you for development and quality control of your products. 4

5 POLICY QUALITY Quality assurance is a top priority in our daily work. The great loyalty of our customers is for us the best proof of their satisfaction. HelioScreen s quality system is an integral part of all operations related to the realization of tests and the sale of substrates. Our laboratory welcomes anyone to audit us with great pleasure. High standards, hard work and excellent quality The quality of our services is the result of excellent study management and successful implementation of scientific findings. Our success is guaranteed not merely by our know-how but rather by the close collaboration of our teams. ONE WRITING SYSTEM Beyond our quality policy, our system is based on the «One Writing System». Since product sending, test perfoming and results receiving, the system ensures totaly traceability without error while writing the information only once at the beginning of the process. The result of In Vitro suncare test in 1 week The strength of our system allows ensuring the realization of the In Vitro tests and sending the preliminary result within less than 1 week. Each step is strictly followed to satisfy customers and keep them informed about the progress of the study as described here below: IN VITRO TEST TIMETABLE Day 0: Reception of the product(s) and the DSBT in our laboratory. Sending of a Reception Sheet including test(s) schedule by . Day 3-5: Study performed. Preliminary Result(s) sent by (PDF form). Day 10-15: Final Report(s) sent by (PDF form). 5

6 INVOLVED RESEARCH As since the begining, each year is strong in innovation and we invite you to find all of our research topics that have been published in our website. Involved in solar field, the goal of our expert team is to propose you new reliable In Vitro tests for sun protection assessment. Scientifc articles, innovation for improvement Our success is based on an increasing demand for the development and testing of cosmetics as well as our scientific expertise, reliability as project partner, decades of continuity and a pioneering concept of developing instruments and methods through practice-oriented research. As an independent contract research organization (CRO) we have already tested, developed and analyzed thousands of products, published numerous scientific papers and participate to several conferences. HelioNews, be in core of solar field HelioNews brings together news in the solar s field and is published by HelioScreen. Published since 1999, discover or re-discover the «News about In Vito Sun Protection Testing» (HelioNews) and take an overview about these interesting following by visiting our website. 6

7 BASIS KNOWLEDGE Through the choice of UV filters, the 3 different values for relevant sun protection and the strategy for suncare product development based on 3 steps, discover the basis of sunscreen summarized in the parts here below. Sunscreen product (Emulsion, Oil, Stick, Powder...) Organic UV filter Inorganic UV filter Sun protection efficiency brought by: - UV filters intrasic performance (quantity, quality, solubilization...) - quality of the film forming (thickness, repartition...) Absorbing effect UV Scattering & Absorbing effect according to particule size UV 3 values for relevant sun protection In order to ensure a relevant sun protection, both UVB and UVA minimal protection must be reached according to 3 different well-known values. SPF Sun Protection Factor (mainly UVB protection) UVA-PF UVA Protection Factor (UVA protection) CW Critical Wavelength (UVB/UVA balance protection) Strategy for suncare product development in 3 steps A global strategy based on 3 different steps helps you to save money, save time, ensure sun protection efficiency for regulations and consumer health safety. 1 st step KNOW YOUR PRODUCT Detect the best formula or raw material performance Estimate the sun protection efficiency for claiming step 2 nd step CLAIM YOUR PRODUCT Test in order to be in compliance with regulation Claim additional sun protection efficiency (Water Resistance...) 3 rd step CONTROL YOUR PRODUCT Check the sun protection efficiency between several batches, ageing, formula

8 SUNCARE TESTING With a wide range of tests covering all needs and also European, Asia, USA and International regulations, HelioScreen assists you in full compliance with the latest standards and official methods. Specific methods are also available to help you for development and quality control of your suncare products. Aako offers high-quality ingredients to the emerging Sun care market. Suntan formulators face a challenging assignment where stable and consistent product quality, undisrupted supplies and compliance with the latest guidelines are key criteria to operate successfully in the market. Axel For Aako HelioScreen has a proven track record regarding in vitro testing for formulations with our ingredients. Besides the technical know-how and state of the art testing facilities, HelioScreen has the flexibility and ability to respond quickly with clear results. All these aspects are giving a big asset to our clients and developers of suntan lotions. HelioScreen is the right partner to involve in all your developments for Sun care! van der Graaf 8

9 WORLDWIDE REGULATIONS Since first sunscreen efficiency evaluation, regulations have continuously changed. In order to have products safe on the market, it is very important to follow up-dated standards and methods. Although a global harmonization is in course, each country has its own particularity. We are pleased to summarize all current standards and methods according to market zone for helping you. AFRICA FDA MONOGRAPH NO ISO YES *according to SANS 1557:2013 ISO YES *according to SANS 1557:2013 ISO YES *according to SANS 1557:2013 FDA MONOGRAPH ASEAN YES YES YES YES YES ASIA EUROPEAN UNION NO *accepted by China & Taiwan YES *excepted China, Korea Japan YES *excepted China & Korea (JCIA PPD method) YES *excepted China NO NO *accepted by China, Korea & Taiwan NO YES YES YES NO *rarely *rarely MERCOSUR NO YES YES YES YES NORTH AMERICA OCEANIA In Vitro Broadspectrum YES YES YES YES YES NO In Vitro UVA-PF & CW YES *according to AS/NZS 2604 In Vivo UVA-PF YES *excepted Australia In Vivo SPF YES *according to AS/NZS 2604 In Vivo SPF YES *excepted Australia USA YES NO NO NO YES Several countries for several regulations. AFRICA: South Africa. ASEAN: Brunei, Cambodia, Indonesia, Laos, Malaysia, Myanmar, Philippines, Singapore, Thailand and Vietnam. ASIA: China, India, Japan, Korea, Russia and Taiwan. EUROPEAN UNION: Austria, Belgium, Bulgaria, Croatia, Cyprus, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Poland, Portugal, Czech Republic, Romania, United Kingdom, Slovakia, Slovenia, Spain and Sweden. MERCOSUR: Argentina, Brazil, Chile, Paraguay, Uruguay and Venezuela. NORTH AMERICA: Canada and Mexico. OCEANIA: Australia and New Zealand. USA: Alaska and United States. Furthermore, improvement of sun protection for consumer leads to propose recommendations of several types: labelling, conditions of use, measurement methodologies of protections values, specific tests of security and stability. Thus, the table here below presents an overview about the sun protection claiming which is often complicated such as each country has one more time its own particularity. SPF protection category Low Medium High Very High UVA Protection (Logo) UVA protection category PA Protection (PA+, PA++, PA+++, PA++++) Boots Star Rating System (***, ****, *****) Broadspectrum UVA-PF 1/3 SPF AFRICA 6, 10 15, 20, 25 30, 40, & CW 370 nm ASEAN UVA labeling not specified but all accepted ASIA EUROPEAN UNION No protection catagory required. Chinese: SPF 2 min - SPF 30+ max India: SPF 50 max Japan: SPF 2 min - SPF 50+ max Korea: SPF 50 max Russia: Tends to align with EU Taiwan: SPF 50+ 6, 10 15, 20, MERCOSUR >50 to <100 Russia: Tends to align with EU UVA-PF 1/3 SPF & CW 370 nm UVA-PF 1/3 SPF & CW 370 nm UVA-PF 1/3 SPF & CW 370 nm This document and information provided herein by HelioScreen does not constitute a legally binding obligation of HelioScreen. Information contained herein are presented in good faith and believed to be accurate, it is provided for guidance only. Please refered to standards/methods and regulations for performing full inspection. 9 India Japan Korea Also recommanded for UK Mexique NORTH 6, 10 15, 20, AMERICA Canada UVA labelling optional - no guidance but all potentially accepted UVA-PF 1/3 SPF OCEANIA 4, 6, 8, 10 15, 20, 25 30, 40, & CW 370 nm USA No protection catagory required. Proposal for SPF 50+ limit in future Broadspectrum 370 nm

10 RELIABILITY EQUIPMENT Currently, evaluation of sun protection is changing and the specificity of this field is more technical than at the begining. HelioScreen is a leader in In Vitro tests for more than 20 years, and develops forefront methods and technologies regarding sunscreen testing. To ensure reliable results, 7 key steps must be mastered and several equipments are required to perform the tests. HelioScreen and HelioScreen Asia Co., Ltd. are the first worldwide laboratories to use these appliances. The 7 Key Parameters of In Vitro suncare testing... Substrate roughness Product amount Temperature control Robotic spreading Solar simulator UV analyzer Calculation + / x -...mastered with our equipment......in our both laboratories. 10

11 DEVELOPMENT KNOW YOUR PRODUCT UVB PROTECTION UVA PROTECTION UVB/UVA BALANCE One of the most well known indicator of UVB protection is based on the SPF. In Vitro SPF evaluation without pre-irradiation In Vitro SPF evaluation with pre-irradiation Beyond the UVB ray light, the UVA-PF is also required in order to prevent sun risk damages. In Vitro UVA-PF evaluation without pre-irradiation In Vitro UVA-PF evaluation with pre-irradiation Equilibrate full protection against UVB and UVA-PF is reached with the CW. In Vitro CW evaluation without pre-irradiation In Vitro CW evaluation with pre-irradiation [He001a] [He001b] [He002b] [He002a] [He003c] [He003b] UVB & UVA PROTECTION QUALITY CONTROL Collect the global protection of your product acording to the SPF, UVA-PF and CW values & assess the photo-stability. In Vitro global pre-testing evaluation for SPF + UVA-PF + CW without irradiation In Vitro global pre-testing evaluation for SPF + UVA-PF + CW with irradiation In Vitro Photostability evaluation [He123 ] [He123b] [He006a] QUALITY CONTROL YOUR PRODUCT A powerful tool for the quality control of batches, Period After-Opening (PAO), product ageing, minor difference in formula... Based on a statistical comparison, check the equivalence between two products of absorbance curve, variability and intensity of sun protection. In Vitro comparison between two products (Published method) [He005b] UV/LIGHT ABSORBANCE Test the light resistance of your product or packaging, evaluate non absorbance of your product or just to measure the UV absorbance curve. In Vitro Color stability evaluation - Test of light resistance In Vitro evaluation of non absorption in the UV In Vitro evaluation of UV absorbance curve in the UV - In solution In Vitro evaluation of UV absorbance curve in the UV - On thin layer [He701] [He802] [He803] [He804] 11

12 REGULATORY CLAIM YOUR PRODUCT UVB PROTECTION Determinate the level of SPF value according to standards for claiming the sun protection of your product. In Vivo* SPF (ISO 24444:2010 method) In Vivo* SPF (FDA 2011 method) [Contact us] [Contact us] Use the UVA-PF value according to standards for claiming the UVA logo or the PA system of your product. UVA PROTECTION In Vitro UVA-PF evaluation with pre-irradiation (ISO 24443:2012 method) In Vitro UVA-PF evaluation with pre-irradiation (Colipa rev method) In Vivo* UVAPF (ISO 24442:2011 method) In Vivo* UVAPF (JCIA 1999 method) [He002c2] [He002c] [Contact us] [Contact us] Beyond the UVA-PF value, the UVB/UVA balance assessment allows claiming the Broadspectrum or the UVA:UVB star rating of your product. UVB/UVA BALANCE In Vitro CW evaluation with pre-irradiation (ISO 24443:2012 method) In Vitro CW evaluation with pre-irradiation (Colipa rev method) In Vitro CW evaluation for Broadspectrum (FDA rev method) In Vitro UVA:UVB ratio - The Boots star rating system (rev. 2011) [He003c2] [He003c1] [He003f] [He003b1] Collect the global protection of your product acording to standards for claiming the SPF, UVA-PF or CW values of your product. UVB & UVA PROTECTION In Vitro UVA-PF (ISO 24443:2012 method) + In Vitro CW with pre-irradiation (ISO 24443:2012 method) In Vitro UVA-PF (Colipa rev method) + In Vitro CW with pre-irradiation (Coliipa rev method) In Vitro SPF + In Vitro UVA PF (ISO method) + In Vitro CW with pre-irradiation (ISO 24443:2012 method) In Vivo* SPF (ISO 24444:2010 method) + In Vitro CW (ISO 24443:2012 method) + In Vitro UVA-PF (ISO 24443:2012 method) [He023c4] [He023c1] [He123c2] [Contact us] In Vivo* SPF (FDA 2011 method) + In Vitro CW for Broadspectrum (FDA rev method) [Contact us] *Tests performed by our external partner 12

13 INNOVATION SPECIAL CLAIM UVB PROTECTION Determinate the level of SPF value according to an in vitro method reproducible, accurate and taking into consideration photo-stability. In Vitro SPF multi-substrates approach (published method) Exclusive [He001c] BEYO ND UV PROTECTION Latest trend and scientific research highlight the need to protect our skin beyond the UV rays. In Vitro Infrared protection assessment (published method) In Vitro Blue Light protection assessment (published method) In Vitro Full Spectrum assessment of a sunscreen product (published method) Exclusive [He807] [He808] [He809] UVB-UVA PHOTOSTABILITY Evalute the UV filters resistance under UV irradiation according to the photostability test. In Vitro Dynamic Photostability evaluation (published method) [He006b] WATER RESISTANCE Evaluate the Water Resistance or Very Water Resistance values for additional claiming of your product. In Vitro Water Resistance evaluation (Published method) In Vitro Very Water Resistance evaluation - Double immersion [He004] [He004a] SUN PROTECTION RESISTANCE Nowadays, beyond Water Resistance, consumers are looking for different sun protection resistances in link to their way of life. In Vitro Rub Resistance of a sunscreen product (published method) In Vitro Wet Skin application of a sunscreeen product (published method) In Vitro assessment of the resistance of the sun protection in extreme conditions (published method) In Vitro Sweat Resistance of a sunscreen product (comming soon) Exclusive [He805] [He806] [He810] [He812] 13

14 SUBSTRATE & EQUIPMENT This part is dedicated to the consummables, equipments and services which are required in order to perfom In Vitro suncare tests. If you are an institute, a cosmetic company, a manufacturer of UV filters, a research laboratory, a university... or for your in-house test, raw material development, new product lunch, claiming test... you will be satisfied with our substrates, our devices and our services. Sanyo Trading is the trading company who offers specialized excellent products in rubber, chemical, machinery and scientific instrument. Sanyo Trading have been providing the requirements for Sun screen testing such as in vitro SPF analyzer, solar simulator, reference sunscreen and substrate. By 5-6 years ago, most of domestic cosmetic companies had been using surgical tape for in vitro Sun screen testing. Many of them had desired consistent, reproducible test substrate at that time. Once new excellent substrate Helioplate was introduced to the cosmetic researchers, Helioplate takes over the fame of most commonly used substrate for Sun screen testing. Helioplate is now supplied to most of cosmetic companies in Japan by Rie Kumemura of Sanyo Trading Sun screen testing team with courteousness service and support. Ken Nishimoto 14

15 SUBSTRATE - HELIOPLATE PMMA MOLDED HD6 From our knowledge and expertise, we have provided the first worldwide substrate recognized for the In Vitro sun protection evaluation. The characteristics of this substrate - roughness and transmission - are in total compliance with all international standards and methods such as ISO 24443:2012 for In Vitro UVA-PF and Critical Wavelength assessment or the FDA rev for Broadspectrum determination... You will improve the reliability of your results with this reproducible substrate. Furthermore, the plate has been specificaly designed in order to avoid roughness damage and to save place for stockage. 2D view 3D view Picture VIEW Manufactured by means of a molding process with our partner La Française des Plastiques, this substrate is delivered with a quality certificate ensuring the reproducibility of roughness. PROCESS Heated PMMA balls Pressure system Mold Mold Lock system Molded plate Surface profile characteristics of the substrate is measured covering at least a surface area of 10 mm x 5 mm in 15-μm intervals. Non-contact surface topographic analysis is conducted using a lab work station consisting of an optical sensor, a motion controller, an x-y translation stage, and microtopography software. A sensor based on a white light chromatic aberration principle is used which allows for a high resolution: 10 nm vertically and 1 um horizontally. QUALITY 15

16 SUBSTRATE - HELIOPLATE PMMA SANDBLASTED SB6 Since last year, our laboratory has proposed you this substrate obtained by a unique sandblasting process. In total compliance with FDA rev method for Broadspectrum assessment, this substrate follows the same principle, the reproducibility of topographics parameters. With the same design than Molded HD6, this substrate will help you to evaluate suncare products which need more carefully. 2D view 3D view Picture VIEW Manufactured by means of a new reproducible sandblasting process, this substrate is delivered with a quality certificate ensuring the reproducibility of roughness as for molded HD6 substrate.. PROCESS Molded plate Sand Sand + Air Air Draft sandblasted Cleaning Air Sandblasted plate Rotation system Surface profile characteristics of the substrate is measured covering at least a surface area of 10 mm x 5 mm in 15-μm intervals. Non-contact surface topographic analysis is conducted using a lab work station consisting of an optical sensor, a motion controller, an x-y translation stage, and microtopography software. A sensor based on a white light chromatic aberration principle is used which allows for a high resolution: 10 nm vertically and 1 um horizontally. QUALITY 16

17 EQUIPMENT HD-THERMASTER The control of the temperature at substrates surface is a key parameter to ensure the reliability of the In Vitro tests. It has been demonstrated that such control is essential during whole process of sunscreen evaluation and not only with the control of the room temperature process. The HD-THERMASTER specifically designed for In Vitro to control and maintain the temperature during whole process. Adapted to receive the HD6 and SB6 substrates, the future In Vitro methods for sun protection evaluation must necessarily take into account the control of the temperature at the substrates surface. Do you want reliable sun protection results? Ask for control of the temperature «Temperature is a key test parameter that should be strictly controlled to ensure reliability.» UV Transmission Assessment: Influence of Temperature on Substrate Surface S. Miksa, D. Lutz and C. Guy Cosmetics & Toiletries, July 2013 Ask us more information HD-THERMASTER By 17

18 EQUIPMENT HD-SPREADMASTER It has been demonstrated that the human spreading provides a significant variability in the results even if the tests are performed in the best conditions. After several months of development, the HD-SPREADMASTER allows total control of sunscreen spreading by means of its concept of robot spreading. Suitable for Molded HD6 and Sandblasted SB6 substrates, this indispensable device specifically dedicated for the In Vitro suncare tests ensures you reproducible results, save time and save money. Do you want reliable sun protection results? Ask for robotic spreading «Reliability and reproducibility are key to the approval and correlation of a new, harmonized test method.» In Vitro UV Testing Robot vs. Human Spreading for Repeatable, Reproducible Results S. Miksa, D. Lutz and C. Guy Cosmetics & Toiletries, October 2013 See the video By 18

19 DEDICATED SERVICES UV SOURCE HelioScreen offers a service for the UV source calibration of your solar simulator. The determination of the UV irradiance and the ratio in total compliance with all international standards and methods such as ISO 24443:2012 for In Vitro UVA-PF and Critical Wavelength assessment or the FDA rev for Broadspectrum determination... UV source calibration [HeS000] UV source calibration HD0 HELIOPLATE These substrates are used to check the linearity and additivity spectrophotometers as required in standard ISO 24443:2012 Annex A. A set of 2 HD0 plates are issued with a certificate and quality measures. Helioplate HD0 [HD0] Linearity & additivity 19

20 TRAINING & COACHING At the heart of the In Vitro UV evaluation since 1999, HelioScreen has acquired in the course of these years a solid and specific experience. As a referent laboratory in this field, we provide our expertise at the service of public health by means of training and coaching. Recognized as a training center by customers, we support you by theoretical and practical sessions of training or coaching. Zurko Research offers high-quality support to Health and Personal care Industries attending to market needs. Conscious of the current relevance of sun protection determination, ZURKO has counted on the support and training of the most experienced and innovative company worldwide concerning in vitro methods, HelioScreen, in order to improve their services. It is surprising to find such small but technologically advanced company, equipped with cutting-edge equipments developed by themselves and pioneer in the proposal of reliable official methods basing on its scientific results. HelioScreen job well done deserves our congratulations and admiration. Passion, perseverance and determination can be noticeable in this kind and qualified team. Mª Engracia Carrión Jiménez 20

21 PRINCIPLES RULES AND REGULATIONS MODULE 1 - FONDAMENTALS Theorical presentation of mechanism for In Vitro testing [HeF000] Aim of the training - Testing methods In Vitro and In Vivo comparison - Technique and limits - Results interpretation Program 1. Presentation of In Vitro test 2. Understand In Vitro test In Vitro test method Principle of solar protection How to protect (Absorption - Scaterring - Biological) Mecanisms involved and evaluation methods In Vivo comparison Key parameters of In Vitro test (Appliances - Substrate - Calibration) Traps to avoid for valid results (Methods - Products - Artefacts) Why In Vitro test? 3. Current methods 4. Practice part In Vivo / In Vitro correlation: Why? How? Limits? Added value of method (global protection-comparion) No treated Theory Demonstration Practice 21

22 LABORATORY TESTS PRACTICES MODULE 2 - DEEPENING Scaling UV transmission and evaluating sun protection [HeF001b] Aim of the training - Existing methods, their specificities - Testing: conditions, control, data treatment Program 1. Understand In Vitro test Impact of Technical & Reglementation aspect on In vitro method development Prerequisite of method vlidation (Difference - Advantage - Inconvenience) 3. Current methods 2. Relevance of results Methodology of each method Key parameters of In vitro test Traps to avoid for valid results (Methods - Products - Artefacts) 4. Practice part How to approach test and ensure reproducibility? Self-validation & control In Vivo / In Vitro correlation & pre-irradiation (study example) Standard method ISO & Broadspectrum (EU or FDA rules) Photostability & pre-irradiation Operational conditions: Tool - Appliance - Sample Tool & Appliance: Calibration - Control Conditions of use of UV-2000S appliance Spreading method Data acquisition Treatment - Interpretation Theory Demonstration Practice 22

23 TESTING IMPROVEMENT AT THE LABORATORY MODULE 3 - DEVELOPMENT Mastering sun protection evaluation / specific method [HeF002b] Aim of the training - Your specific questions - Your laboratory practical difficulties - The specific methods Program 1. Understand In Vitro test Reasons of reproducibility difficults Know how to deflect practical difficults: Advantage - Inconvenience 3. Current methods Standard ISO application Broadspectrum evaluation according to EU or FDA rules Photostability and pre-irradiation 2. Relevance of results Diversity of methods How ensure best use of in vitro results Evaluation method through measurement difficulties 4. Practice part Operational conditions: Tool - Appliance - Sample Tool & Appliance: Calibration - Control Spreading method Data acquisition Treatment - Interpretation Theory Demonstration Practice 5. Your issues Questions Specific methods Practical lab difficulties 23

24 Bangkok Lab QUALITY RESEARCH KNOWLEDGE HelioScreen Europe 44, rue Leon Blum Creil - France Tel: +33(0) commercial@helioscreen.fr HelioScreen Asia Co., Ltd. CAHB building - 49 Soi Phokaew3 Yak19, Phokaew Road, Klongchan, Bangkapi, Bangkok Thailand Tel: +66(0) pkadeewee@helioscreenasia.com