TAIEX Study Visit on Determination of Polycyclic Aromatic Hydrocarbons (PAH) and Veterinary Drugs Residues

Transcription

1 TAIEX Study Visit on Determination of Polycyclic Aromatic Hydrocarbons (PAH) and Veterinary Drugs Residues organised in co-operation with Spanish Agency for Consumer Affairs, Food Safety and Nutrition of Ministry of Health, Social Services and Equality Venue : Centro Nacional de Alimentación Carretera Pozuelo-Majadahonda, km 5, Majadahonda Madrid April 2017 Beneficiary : Montenegro AGR IND/STUD 64064

2 Aim of the visit: The aim of the visit is to get practical knowledge on both the implementation of the determination of the 4 PAH covered by the European legislation (Regulation (UE) No 1881/2006) and on the analysis of veterinary drug residues included in groups A1 (stilbens), A3 (steroids), A4 (resorcylic acid lactones) and A5 (beta-agonists) as it is stablished in European legislation (Directive 2008/97/CE amending Council Directive 96/22/EC, Council Directive 96/23/EC, Commission Decision 2002/657/EC). Accordingly the study visit activities are organised in two parts: The aim of the first part is to get practical knowledge of the determination of the 4 PAH covered by the European legislation (Regulation (UE) No 1881/2006, as amended). The method of analysis is based on direct extraction / dispersion of samples with the extraction solvent. The clean-up of the crude extracts is carried out by size exclusion chromatography followed by HPLC with fluorescence detection as described in the UNE CEN/TS This part is completed by a series of theoretical sessions where aspects such us the in-house validation, use of alternative extraction solvents, checking of performance characteristics according to Regulation (UE) No 836/2011, performance requirements for the sum of analytes, quality control and preparation of reports of analysis, will be discussed. Finally, some general aspects will be presented: toxicology, occurrence and alerts in the EU. The aim of the second part of the visit is to get practical knowledge about the analysis of veterinary drug residues included in groups A1 (stilbens), A3 (steroids), A4 (resorcylic acid lactones) and A5 (beta-agonists) as it is stablished in European legislation (Directive 2008/97/CE amending Council Directive 96/22/EC, Council Directive 96/23/EC, Commission Decision 2002/657/EC, etc.). Two analytical methods will be presented in order to cover these 4 groups. Basically, both methods consist of three steps: - Enzymatic hydrolysis and extraction - Solid phase extraction (SPE) clean-up - Liquid chromatography coupled to mass spectrometry in tandem detection (LC- MS/MS). The visit will be completed by theoretical sessions where aspects such as in-house validation according to Commission Decision 2002/657/EC, target matrices, quality control and other technical topics will be discussed.

3 Day 1 : Monday 03 April 2017 Chair : Pedro A. Burdaspal 08:30 Welcome and introduction to CNA and the study visit 09:30 Theoretical session 1: Presentation of the analytical procedure used in the CNA. 10:00 Laboratory session 1A: Analysis of PAH in edible oil including recovery experiments. 11:00 Coffee break 11:30 Theoretical session 2: EU legislation on PAH. Method performance requirements in PAH determination. In-house validation and quality control 13:30 Laboratory session 1B: Continuation of the analysis of PAH in edible oil. Data processing 15:30 End of the first day 16:00 Departure from CNA

4 Day 2 : Tuesday 04 April 2017 Chair : Pedro A. Burdaspal 08:30 Laboratory session 2: Analysis of PAH in meat products containing smoked ingredients. 10:30 Coffee break 11:00 Theoretical session 3: General aspects on PAH: Toxicology, occurrence and alerts in the EU 12:15 Theoretical session 4: Discussion of results from laboratory sessions 1 and 2. 13:45 Preparation of analytical reports 16:00 End of second day Day 3 : Wednesday 05 April 2017 Chair : José Blanca 08:30 Theoretical session 5: Analysis of anabolic hormones by LC-MS-MS (groups A1, A3, A4) 09:30 Coffee break 10:00 Laboratory session 3: Analysis of anabolic hormones in urine: sample preparation, incubation/extraction and clean-up 12:30 Theoretical session 6: Technical aspects. 13:30 Laboratory session 4: Results on LC-MS/MS equipment and discussion. 16:00 End of third day

5 Day 4 : Thursday 06 April 2017 Chair : José Blanca 08:30 Theoretical session 7: Analysis of betaagonists by LC-MS/MS (A5) 09:30 Coffee break 10:00 Laboratory session 5: Analysis of beta-agonists in urine: sample preparation, incubation/extraction and clean-up 12:30 Theoretical session 8: Technical aspects. 13:30 Laboratory session 6: Results on LC-MS/MS equipment and discussion. 16:00 End of fourth day Day 5 : Friday 07 April 2017 Chair: José Blanca y Patricia Muñoz 08:30 Theoretical session 9: In-house validation according to CD 2002/657/EC. 09:30 Coffee break 10:00 Theoretical session 10: In-house validation according to CD 2002/657/EC. 11:00 Quality Control 12:00 Theoretical session 11: Technical aspects and discussion. 14:15 Summary of whole study visit and closing meeting 15:30 End of fifth day

6 This meeting is being organised by the Technical Assistance Information Exchange Instrument of the European Commission TAIEX on Twitter: #EUTaiex Telephone: