Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate.

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1 ExProfile TM Human Immune Complement Related Gene qpcr Array For focused group profiling of human immune complement genes expression Cat. No. QG033-A (1 x 96-well plate, Format A) Cat. No. QG033-B (1 x 96-well plate, Format B) Cat. No. QG033-C (1 x 96-well plate, Format C) Cat. No. QG033-D (1 x 96-well plate, Format D) Cat. No. QG033-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human immune complement related gene qpcr array profiles the expression of 84 human genes related to immune complement. These genes are carefully chosen for their close correlation based on a thorough literature search of peer-reviewed publications, mainly including genes that encode major components of the classical, alternative and lectin pathways, such as accessory molecules and multiple complement control proteins. This array allows researchers to study the related genes to gain understanding of their roles in the functioning and characterization of immune complement. QG033 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG033 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ TPO QG A02 HQP NM_ THBS1 QG A03 HQP NM_ SPP1 QG A04 HQP NM_ SIRPA QG A05 HQP NM_ SERPINE1 QG A06 HQP NM_ PROS1 QG A07 HQP NM_ PLG QG A08 HQP NM_ PLAU QG A09 HQP NM_ PLAT QG A10 HQP NM_ NR1H4 QG A11 HQP NM_ MMP14 QG A12 HQP NM_ MBL2 QG B01 HQP NM_ MASP2 QG B02 HQP NM_ LRP1 QG B03 HQP NM_ KRT1 QG B04 HQP NM_ KLK1 QG B05 HQP NM_ ITGB3 QG B06 HQP NM_ ITGB2 QG B07 HQP NM_ ITGAX QG B08 HQP NM_ ITGAV QG B09 HQP NM_ ITGAM QG B10 HQP NM_ IFNA7 QG B11 HQP NM_ HRG QG B12 HQP NM_ GC QG C01 HQP NM_ FCN1 QG C02 HQP NM_ CRP QG C03 HQP NM_ CR2 QG C04 HQP NM_ CPN1 QG C05 HQP NM_ CLU QG C06 HQP NM_ CFP QG C07 HQP NM_ CFHR5 QG C08 HQP NM_ CFHR4 QG C09 HQP NM_ CFH QG C10 HQP NM_ CFD QG C11 HQP NM_ CFB QG C12 HQP NM_ CD97 QG D01 HQP NM_ CD93 QG D02 HQP NM_ CD59 QG D03 HQP NM_ CD55 QG D04 HQP NM_ CD46 3

4 4 Product Data Sheet QG D05 HQP NM_ CD44 QG D06 HQP NM_ CD209 QG D07 HQP NM_ CAPRIN2 QG D08 HQP NM_ C9 QG D09 HQP NM_ C8G QG D10 HQP NM_ C8B QG D11 HQP NM_ C8A QG D12 HQP NM_ C7 QG E01 HQP NM_ C6 QG E02 HQP NM_ C5AR1 QG E03 HQP NM_ C5 QG E04 HQP NM_ C4BPB QG E05 HQP NM_ C4BPA QG E06 HQP NM_ C4A QG E07 HQP NM_ C3AR1 QG E08 HQP NM_ C3 QG E09 HQP NM_ C2 QG E10 HQP NM_ C1S QG E11 HQP NM_ C1RL QG E12 HQP NM_ C1R QG F01 HQP NM_ C1QL4 QG F02 HQP NM_ C1QL3 QG F03 HQP NM_ C1QL1 QG F04 HQP NM_ C1QC QG F05 HQP NM_ C1QBP QG F06 HQP NM_ C1QA QG F07 HQP NM_ C13orf15 QG F08 HQP NM_ BGN QG F09 HQP NM_ APCS QG F10 HQP NM_ C1QL2 QG F11 HQP NM_ CSMD1 QG F12 HQP NM_ IBSP QG G01 HQP NM_ MASP1 QG G02 HQP NM_ CFHR1 QG G03 HQP NM_ CFHR2 QG G04 HQP NM_ CFHR3 QG G05 HQP NM_ CFI QG G06 HQP NM_ CPB2 QG G07 HQP NM_ CR1 QG G08 HQP NM_ PRF1 QG G09 HQP NM_ DCN QG G10 HQP NM_ FAM132A QG G11 HQP NM_ FMOD QG G12 HQP NM_ GPR77 QG H01 HGDC QG H02 HGDC

5 QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Following terms and conditions apply to use of ExProfile TM Human Immune Complement Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG