Dehydrated Media. Advantages. Application. Simple handling. Long shelf life. High reproducibility. Less risk of contamination

Transcription

1 NutriDisk Dehydrated Media Products Advantages Simple handling Long shelf life High reproducibility Less risk of contamination Application The NutriDisk: set contains everything. 72

2 Products NutriDisk Dehydrated Media Membrane filters with nutrient pads for microbiological testing Application Soft drinks Acid-tolerant microorganisms Colony count Lactic acid bacteria Slime formers (Leuconostoc) Yeast and mold Beer and wine Pediococcus, Lactobacillus and Zymomonas Wild yeast Yeast and mold Wine spoiling microorganisms Water Colony count Escherichia coli and coliform Streptococcus Pseudomonas aeruginosa Foodstuffs Colony count Colony count for dairy products Enterobacteria Lactobacillus Mesophilic bacteria and thermophilic spore-formers Acid-tolerant microorganisms Salmonella Staphylococcus Streptococcus Pseudomonas aeruginosa Sugar Mesophilic bacteria and thermophilic spore-formers Osmophilic yeast Slime formers (Leuconostoc) Yeast and mold Pharmaceutical and cosmetics Colony count Pseudomonas aeruginosa Staphylococcus Yeast and mold NutriDisk type Orange Serum Standard TTC or Standard Plate Count Beer or MRS Weman Wort or Schaufus Pottinger Beer or MRS Lysin Wort Wine Standard-TTC or Standard Plate Count Endo, Teepol or M-FC Azide Cetrimide Standard-TTC, Standard, Caso or Plate Count China Blue Lactose Endo, Teepol, M-FC, Tergitol TTC or ECD MRS Glucose-Tryptone Orange Serum Bismuth Sulphite Chapman Azide Cetrimide Glucose-Tryptone Osmophile Weman Wort or Schaufus Pottinger Caso or Schaedler Cetrimide Chapman Sabouraud 73

3 NutriDisk Dehydrated Media Products Orange Serum Nutrient Pad Intended Use: Medium for the isolation, cultivation and enumeration of acid-tolerant, putrafacation microorganisms in fruit juices and fruit juice concentrates, especially those from citrus fruits. This culture medium is in accordance with the recommendations of the German Institut für Lebensmitteltechnologie und Verpackung (Institute for Food Technology and Packaging 1974). Recommended Incubation Time: 3 5 days at C Incubate the inoculated culture medium up to 3 days; if fungi are suspected to be present, incubate up to 5 days. Determine the microbial count. Further tests can be performed to differentiate and identify the colonies. Additional information: The culture medium is optimally adapted to the special requirements of the microbial flora present in citrus juice (e.g. Bacillus, Lactobacillus, Leuconostoc species, fungi, etc.) due to the fact that it contains orange extract. It is therefore especially useful for production control in the fruit juice industry. Orange Serum Nutrient Pad Ingredients per liter of demineralised water adjusted to ph 5.5 ± 0.2 at 25 C. Peptone from Casein 30 g Yeast extract 9.0 g Orange extract 15 g D(+) glucose 12 g Di-potassium hydrogen phosphate 9 g Schaufus Pottinger Nutrient Pad Intended Use: For the detection and determination of the total count of yeast and mold in beverages and sugar according to Schaufus and Pottinger. Mold develops velvety or fluffy whitish to greenish colonies which can take on various colors after conidiospore production. Yeast and bacteria colonies have smooth surfaces. Acid forming sugar fermenters have a whitish to yellow coloration; non acid formers are, by contrast, greenish to blue green. Select characteristic colonies from Schaufus Pottinger plates and perform additional physiological and serological tests as required. Note that colonial morphology as observed macroscopically is a presumptive aid and does not provide information for specification. Recommended Incubation Time: 2 3 days at C. Ingredients per liter of demineralised water adjusted to ph 5.6 ± 0.1 at 25 C. Yeast Extract Glucose (Dextrose) Polypeptone (Universal Peptone) Magnesium Sulfate Potassium Phosphate Amylase Thiamine Bromcresol green 27 g 150 g 30 g 6.30 g 6.0 g 0.15 g 0.15 g 78 mg Product Colour/ Grid Pore size [µm] Size [mm] Qty/Pkg Order No 74 Orange Serum green / black NutriDisk STL Orange Serum STL* green / black Schaufus Pottinger green / black NutriDisk STL Schaufus Pottinger STL* green / black * Membranes are packed in a zigzag format

4 Products NutriDisk Dehydrated Media Standard Plate Count Nutrient Pad Intended Use: Standard Plate Count Media is recommended for use in determining the Standard Plate Count of viable bacteria in milk, dairy products, and other material of sanitary significance. Standard Plate Count Media is formulated according to specifications from the APHA for use in performing the Standard Plate Count for bacteria in dairy products, and the same formulation described for Plate Count Agar, dehydrated (Tryptone Glucose Yeast Agar) in the Standard Methods for the Examination of Water and Wastewater 13 th, Edition 4. It is also the same formulation as the Tryptone Glucose Yeast Agar from the Association of Official Analytical Chemist (AOAC) and conforms to the formulation specified by the U.S. Pharmacopea. Recommended Incubation Time: 2 5 days at 25 C. Historical background: Standard Plate Count Media is a modification of Bowers and Hucker Tryptone Glucose Skim Milk Agar. It was shown by Dr. Yale (approx. 1938) to be more effective in plate count procedures for milk and dairy products. The American Public Health Association (APHA) in their Standard Methods for the Examination of Dairy Products recommends the use of Standard Plate Count Media for the performance of the Standard Plate Count on dairy products. Standard Agar is the same formulation as Plate Count Agar recommended by the APHA for use in the Standard Plate Count for water. Standard Plate Count Nutrient Pad Ingredients per liter of demineralised water adjusted to ph 7.0 ± 0.2 at 25 C. Peptone (Pancreatic Digest of Casein) Yeast Extract Dextrose 5.0 g 2.5 g 1.0 g Standard TTC Nutrient Pad Intended Use: For the determination of the colony count of water and waste water; for pure culture and as a base medium for further additions to demineralised water (serum, antibiotics etc.) by wetting the pads. Recommended Incubation Time: hours at 20 C or 48 hours at 30 C. Additional information: 2, 3, 5-Triphenyltetrazolium chloride (TTC) is rapidly reduced to red formazan by almost all coliform organisms including Escherichia coli. This phenomenon allows early detection of the red Escherichia coli colonies. Ingredients per liter of demineralised water adjusted to ph 7.4 ± 0.1 at 25 C. Peptone (Pancreatic Digest of Casein) Yeast Extract NaCl D(+) glucose TTC (2, 3, 5-Triphenyltetrazolium chloride) 45 g 9 g 18 g 3 g 0.3 g Product Colour/ Grid Pore size [µm] Size [mm] Qty/Pkg Order No NutriDisk Plate Count green / black NutriDisk STL Plate Count STL* green / black NutriDisk Standard TTC green / black NutriDisk STL Standard TTC STL* green / black * Membranes are packed in a zigzag format 75

5 NutriDisk Dehydrated Media Products Wort Nutrient Pad Intended Use: For the detection of yeast and mold in beverages, food and other products. For the cultivation, isolation and enumeration or enrichment of fungi, especially of yeast. Recommended Incubation Time: 2 5 days at 25 C Additional information: The accompanying bacterial flora is weakly suppressed by a ph value of 5.0. Evaluation: Yeast usually develop as smooth white or colored colonies. Mold, as a rule, form velvety or fluffy, cotton-like colonies which are white in the early growth phase and may later take on various colors after conidiospore production. Select characteristic colonies from wort plates and perform additional physiological and serological tests as required. Note that colonial morphology as observed macroscopically is a presumptive aid and does not provide information for specification. Wort Nutrient Pad Ingredients per liter of demineralised water adjusted to ph 5.0 ± 0.2 at 25 C. Malt Extract 45 g Universal Peptone (Polypeptone Peptone) 2.25 g Maltose g Dextrin 8.25 g Glycerol 7.05 g Potassium dihydrogen phosphate 1.2 g Ammonium chloride 3 g Product Colour/ Grid Pore size [µm] Size [mm] Qty/Pkg Order No NutriDisk Wort white / black NutriDisk STL Wort* white / black * Membranes are packed in a zigzag format 76

6 Products NutriDisk Dehydrated Media Membrane filters with nutrient pads for microbiological testing Azide NutriDisk Selective medium for the detection of fecal Streptococci in water and foodstuffs. Recommended incubation conditions: hr / 37 C. Beer NutriDisk Selective medium for the detection of beer-spoiling microorganisms including Lactobacillus, Pediococcus and Zymomonas sp. The growth of yeast is suppressed by Actidione (cycloheximide). Recommended incubation conditions: 2 5 days / 30 C anaerobic. Caso NutriDisk For determination of the colony counts and detection of fastidious or sublethally damaged microorganisms in pharmaceutical or cosmetic products. (USP). Recommended incubation conditions: hr / 30 C. Cetrimide NutriDisk Selective medium for the detection of Pseudomonas aeruginosa in water, pharmaceutical and cosmetic products (USP, DIN). Recommended incubation conditions: 48 hr / 37 C. Chapman NutriDisk Selective medium for the detection of pathogenic Staphylococci in foodstuffs, pharmaceutical, cosmetic and clinical samples (USP, APHA). Recommended incubation conditions: 48 hr / 37 C. China Blue Lactose NutriDisk For determination of the colony count and for differentiation of acid formers and non-acid formers in milk and diary products. Recommended incubation conditions: hr / 30 C. ECD NutriDisk with Indolkit Selective medium for the direct and quantitative detection of Escherichia coli in water and foodstuffs within 24 hr. Recommended incubation conditions: hr / 44 C. Endo NutriDisk Selective medium for the detection of Escherichia coli and coliform in water and foodstuffs (DEV, APHA). Recommended incubation conditions: 24 hr / 37º C or 42 44º C. Glucose-Tryptone NutriDisk For determination of the colony count of mesophilic microorganisms and the detection of thermophilic spore formers in sugar and foodstuffs (ICUMSA, NCA). Recommended incubation conditions: 2 3 days / 30 C or 1 2 days / 55 C. Lysin NutriDisk Selective medium for the detection of wild yeast in breweries (according to MORRIS and EDDY). Cultured yeast and wild yeast of the Saccharomyces family do not grow. Recommended incubation conditions: 2 5 d ays / C. M-FC NutriDisk Selective medium for the detection of Escherichia coli and fecal coliform in water and foodstuffs. Recommended incubation conditions: hr / 44 C. MRS NutriDisk Selective medium for the detection of Lactobacilli in foodstuffs. Yeast and mold are suppressed by Actidione (cycloheximide). Recommended incubation conditions: 3 5 days / 30 C anaerobic. Osmophile NutriDisk For the detection of osmophilic and osmotolerant yeast and mold in sugars and foodstuffs containing sugars (ICUMSA). Recommended incubation conditions: 5 7 days / 25 C. Self-filling syringe For wetting the NutriDisk with sterile water. Autoclavable at 121 C. The cylinder fills itself automatically each time the adjustable filling volume has been dispensed. Self-filling syringe. 77

7 NutriDisk Dehydrated Media Membrane filters with nutrient pads for microbiological testing Products Resuscitation NutriDisk without membrane filter For the detection of sublethally damaged Enterobacteria, Pseudomonas and Staphylococci by pre-incubation and further incubation on selective media. Recommended incubation conditions: 2 4 hr / 37 C or hr / 20 C. Sabouraud NutriDisk For the detection of yeast and mold in pharmaceutical and cosmetic products, packaging materials as well as for the isolation of dermatophytes and for pure cultures (USP). Recommended incubation conditions: 2 5 days / C. Schaedler NutriDisk For the determination of the colony count and for the detection of fastidious aerobic and anaerobic microorganisms in clinical, pharmaceutical and cosmetic samples. Recommended incubation conditions: hr / 30 C. Teepol NutriDisk Selective medium for the detection of Escherichia coli and fecal coliform in water and foodstuffs. By pre-incubation (6 hr at 25 C) even sublethally damaged microorganisms can be easily recovered. Recommended incubation conditions: hr / 44 C. Tergitol-TTC NutriDisk Selective medium for the detection of Escherichia coli and fecal coliform in water and foodstuffs. Recommended incubation conditions: hr / 37 C. Wine NutriDisk Selective medium for the detection of wine-spoiling microorganisms, including Leuconostoc, Lactobacillus and Pediococcus sp. Improved tomato juice medium. Under aerobic conditions Acetobacter sp. can also be detected. Recommended incubation conditions: 2 5 days / 30 C anaerobic. Weman NutriDisk For the detection of slime-forming bacteria in sugar, soft drinks and foodstuffs (ICUMSA). Recommended incubation conditions: 2 3 days / 30 C. Bismuth Sulphite NutriDisk Selective medium for the detection of Salmonella sp. in water, foodstuffs and clinical samples (USP, APHA). Recommended incubation conditions: h r/ 37 C. 78

8 Products NutriDisk Dehydrated Media Product Color/ Grid Pore size [µm] Size [mm] Qty/Pkg Order No NutriDisk Azide white / black NutriDisk Beer green / black NutriDisk Caso green / black NutriDisk STL Caso green / black NutriDisk Cetrimide white / black NutriDisk STL Cetrimide white / black NutriDisk STL Cetrimide white / black NutriDisk Chapman white / black NutriDisk China blue lactose white / black ECD NutriDisk with Indolkit white / black NutriDisk Endo white / black NutriDisk STL Endo white / black NutriDisk STL Endo white / black NutriDisk Glucose-Tryptone white / black NutriDisk Lysin green / black NutriDisk M-FC white / black NutriDisk STL M-FC white / black NutriDisk MRS green / black NutriDisk Osmophile green / black NutriDisk Plate Count green / black NutriDisk STL Plate Count green / black NutriDisk Sabouraud green / black NutriDisk Schaedler green / black NutriDisk Teepol white / black NutriDisk Tergitol-TTC white / black NutriDisk Wine green / black NutriDisk Weman green / black NutriDisk Bismuth sulphite white / black NutriDisk Resuscitation without membrane filter Petri dishes with pads, sterile Petri dishes with pads, sterile * Self-filling syringe * available on request 79

9 Rapid Tests Contamination tests using the membrane filtration method Yeast and Lactobacillus Rapid Test Quantitative Rapid Tests The Yeast and Lactobacillus Rapid Tests are based on the membrane filtration method. In addition the quantitative results are available within 24 hours, rapid determination of yeast contamination within 8 hours. Products Yeast Rapid Test For the rapid detection of yeast contamination in soft drinks. Yeast concentrations of 1000 yeast cells/ml can be detected in only 8 hours. The membrane filter is incubated on a nutrient medium and colored blue with reagent solution. After only 24 hours incubation even a single colony can be detected. Package includes: 50 membrane filters, reagent solution, special nutrient media pads for 50 tests. Yeast and Lactobacillus Rapid Test. Lactobacillus Rapid Test For the rapid detection of Lactobacillus contamination in soft drinks. In the microbiological spoilage of soft drinks, carbonated soft drinks in particular, Lactobacilli play an important role. In the LST-45 test the membrane filter is incubated on a nutrient medium and the colonies are then colored blue with a reagent solution. Even dense growth can be easily recognized. After a maximum of 24 hours even a single colony can be detected on the membrane filter. Package includes: 50 membrane filters, reagent solution, special nutrient media pads for 50 tests. Product Description Qty/Pkg Order No HST-45 Yeast Rapid Test LST-45 Lactobacillus Rapid Test

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11 Swabs SwabCheck Citrus-S Products SwabCheck Citrus-S For the presumptive identification of Escherichia coli on production and product contact surfaces in the citrus industry. Description: This media was specifically developed for the detection of Escherichia coli on all surfaces that a product may come into contact with, such as peeling knives, rotary cutters, filling heads, cappers, pulpers, squeezers etc. The media is modified from that published by Feng and Hartman by the addition of MUG. The test kit uses neutralizing buffer as the sample collection medium. The neutralizing buffer inactivates chlorine and quaternary ammonium based detergents that may be present on a surface after the implementation of a cleaning protocol. It allows residual bacteria whose growth may be suppressed, but not killed, to develop and be identified. Organisms Characteristics E. coli ATCC Growth/Fluoresces E. aerogenes ATCC Growth/No fluorescence E. faecalis ATCC Inhibited Lactose in the media acts as a source of fermentable carbohydrates for coliforms. Casein provides additional nutrients while sodium chloride maintains the osmotic equilibrium of the media. Bile salts inhibit gram-positive bacteria, especially bacilli and fecal streptococci. The substrate 4-Methylumbelliferyl-β- D-glucuronide (MUG) is hydrolyzed by the enzyme β-glucuronidase which is specific to Escherichia coli within the coliform group of bacteria. This reaction yields a fluorescent end product, 4-Methylumbelliferone, which fluoresces blue on exposure to long wavelength UV light (typically 366 nm). Neutralizing buffer inactivates the bactericidal and bacteriostatic effects of both chlorine and quaternary ammonium based detergents. Neutralizing buffer exhibits no toxic effects on microorganisms. Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, incubated at 37ºC for 24 hours, Fluoresces with UV. Negative control: Not undertaken. Sterility: 7 days plated sterility test. SwabCheck Citrus-S. Formulation Media: Per liter of water adjusted to ph 6.9 ± 0.2 Pancreatic Digest of Casein Lactose Bile salts mixture Dipotassium phosphate Monopotassium phosphate Sodium chloride 4-Methlyumbelliferylβ-D-glucuronide Formulation Collection Buffer: Per liter of water adjusted to ph 7.2 ± 0.2 Monopotassium phosphate Sodium thiosulfate Aryl sulfonate complex 20.0 g 5.0 g 1.5 g 4.0 g 1.5 g 5.0 g 50 mg 42.5 mg 0.16 g 5.0 g Product Description Qty/Pkg Order No SwabCheck Citrus-S pack

12 Products Swabs SwabCheck Citrus-J SwabCheck Citrus-J For the presumptive identification of Escherichia coli in citrus juices. Description: This media was specifically developed for the detection of Escherichia coli in citrus juices and is modified from that published by Feng and Hartman by the addition of MUG. A sterile swab is provided for sampling the juice. Lactose in the media acts as a source of fermentable carbohydrates for coliforms. Casein provides additional nutrients while sodium chloride maintains the osmotic equilibrium of the media. Bile salts inhibit gram-positive bacteria, especially bacilli and fecal streptococci. The substrate 4-Methylumbelliferyl-β- D-glucuronide (MUG) is hydrolyzed by the enzyme β-glucuronidase which is specific to Escherichia coli within the coliform group of bacteria. This reaction yields a fluorescent end product, 4-Methylumbelliferone, which fluoresces blue on exposure to long wavelength UV light (typically 366 nm). Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, incubated at 37º C for 24 hours, Fluoresces with UV. Negative control: Not undertaken. Sterility: 7 days plated sterility test. SwabCheck Citrus-J. Per liter of water adjusted to ph 6.9 ± 0.2 Pancreatic Digest of Casein Lactose Bile salts mixture Dipotassium phosphate Monopotassium phosphate Sodium chloride 4-Methlyumbelliferylβ-D-glucuronide 20.0 g 5.0 g 1.5 g 4.0 g 1.5 g 5.0 g 50 mg Organisms Characteristics E. coli ATCC Growth/Fluoresces E. aerogenes ATCC Growth/No fluorescence E. faecalis ATCC Inhibited Product Description Qty/Pkg Order No SwabCheck Citrus-J pack

13 Swabs Neutralizing Buffer Swabs and SwabCheck Products Neutralizing Buffer Swabs Neutralizing buffer inactivates the bactericidal and bacteriostatic effects of chlorine and quaternary ammonium detergents. However neutralizing buffer exhibits no toxic effects on microorganisms. This permits the transfer of swabbed organisms to the laboratory without loss in viability. Description: Neutralizing buffer swabs are used in the monitoring of surfaces for total bacterial count. Neutralizing buffer is not designed to culture and enumerate microorganisms. Quality Control and recommended incubation conditions: Positive control: Undertaken on cultured organisms after transfer to standard methods agar plates from neutralizing buffer. Escherichia coli ATCC 25922, incubated at 35º C for 24 hours. Negative control: Not undertaken. Sterility test: 7 days plated sterility test. Neutralizing Buffer Swabs. Per liter of water adjusted to ph 7.2 ± 0.5 Monopotassium phosphate Sodium thiosulfate Aryl sulfonate complex 42.5 g 160 mg 5.0 g SwabCheck Used as an indication of hygiene on contact surfaces. Description: SwabCheck changes color from purple to yellow. The color change is based on an acid reaction with the indicator. The more rapid the color change the higher the level of bacteria in the sample. SwabCheck is useful in determining the sanitation levels of preparation surfaces, filling ports and processing areas in beverage and food processing plants, dairies, restaurants and healthcare facilities. The presence of bacteria is indicated by a color change from purple to yellow. Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, hours at 35 37º C. Negative control: Not undertaken. Sterility: 7 days plated sterility test. SwabCheck. Right: control tube; left: Escherichia coli ATCC is indicated by a change in color from purple to yellow. Proprietary Product Description Qty/Pkg Order No Neutralizing Buffer Swabs 4 ml ml SwabCheck 4 ml ml

14 Products Swabs Letheen Swabs Letheen Swabs Letheen swabs are used for the determination of bactericidal activity of quaternary ammonium compounds. Description: Letheen broth, on which the swab systems are based, is a modification of the AOAC formula. Letheen broth incorporates lecithin to neutralize the effects of quaternary ammonium compounds and sorbitan monooleate to neutralize phenolic disinfectants, hexachlorophene and formalin. Lecithin and sorbitan monooleate synergistically react to neutralize the effects of ethanol. Inaccurate counts would be obtained if the disinfectants were allowed to mask the presence of organisms by means of their bacteriostatic action. Quality Control and recommended incubation conditions: Undertaken on cultured organisms after transfer to standard methods agar. Positive control: Escherichia coli ATCC 25922, incubated hours at 35º C. Negative control: Not undertaken. Sterility test: 7 days plated sterility test. Letheen Swabs. Per liter of water and adjusted to ph 7.0 ± 0.2 Proteose Peptone #3 Beef extract Lecithin Polysorbate 80 Sodium chloride 10 g 5 g 0.7 g 5 g 5 g Letheen Swabs will allow total count recoveries from surfaces that have previously been sanitized using any of the standard sanitizing agents, where the presence of the sanitizing agent would mask the recovery of the organisms. Growth obtained after incubation can be examined for colonial morphology and subcultures made for identification purposes. Product Description Qty/Pkg Order No Letheen Swabs 4 ml ml

15 Buffer Swabs and ColiCheck Products Buffer Swabs Used for the collection of surface contamination from flat or convoluted surfaces prior to transport to a laboratory for culture and enumeration. Description: Buffer swabs contain no bacteriostatic or bactericidal compounds and cannot suppress the action of detergents None required. ColiCheck Quality Control and recommended incubation conditions: Undertaken on cultured organisms after transfer to standard methods agar plates from the buffer solution. Positive control: Escherichia coli ATCC 25922, incubated at 35º C for 24 hours. Negative control: Not undertaken. Sterility: 7 days plated sterility test. BufferSwabs. Make to 1 liter and adjust ph to 7.2 ± 0.5 Stock solution 1.25 ml/l Potassium di-hydrogen phosphate 34 g Used for the presumptive identification of coliforms in water samples by a presence/absence technique. Description: Bromocresol purple is a ph indicator which demonstrates a color change from purple to yellow in the presence of acid. Lactose fermenting organisms produce acid which initiates the color change. The presence of coliforms is detected with greater sensitivity by use of a relatively large sample volume (100 ml) in a single bottle. Yellow color in the sample after 24 hours incubation at 35 C indicates the presence of coliform organisms. A red sample after 24 hour incubation at 35 C should be incubated for further 24 hours. A red sample at 48 hours incubation at 35 C indicates no coliform organisms present in the sample. Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, incubated at 35 C for 48 hours. Per liter of water and adjusted to ph 6.8 ± 0.2 Beef extract Pancreatic Digest of Gelatin Lactose Pancreatic Digest of Casein Dipotassium phosphate Monopotassium phosphate Sodium chloride Sodium lauryl sulfate Bromocresol purple 3.0 g 5.0 g 7.5 g 10.0 g g g 2.5 g 50 mg 8.5 mg Organisms Characteristics Coloring E. coli ATCC Growth Yellow E. aerogenes ATCC Growth Yellow E. faecalis ATCC Inhibited Red Negative control: Sterile water incubated at 35 C for 48 hours. Sterility test: 14 days plated sterility test. Product Description Qty/Pkg Order No Buffer Swabs 4 ml Buffer Swabs 4 ml ColiCheck P/A test kit with sample bottles ColiCheck P/A test

16 Products ColiCheck with MUG ColiCheck with MUG Used for the presumptive identification of coliforms and the determination of the presence of Escherichia coli in water samples by a presence/absence technique. Description: Bromocresol purple is a ph indicator which demonstrates a color change from purple to yellow in the presence of acid. Lactose fermenting organisms produce acid which initiates the color change. The presence of coliforms is detected with greater sensitivity by use of a relatively large sample volume (100 ml) in a single bottle. The addition of MUG (4-Methylumbellifryl-β-D-glucuronide) which is a fluorogenic enzyme allows the media to selectively identify Escherichia coli. MUG is hydrolyzed by the Escherichia coli specific enzyme β-glucuronidase to release 4-Methylumbelliferone which fluoresces under ultraviolet light (approx. 366 nm wavelength). Yellow color in the sample after 24 hours incubation at 35 C indicates the presence of coliform organisms. A red sample after 24 hour incubation at 35 C should be incubated for further 24 hours. A red sample at 48 hours incubation at 35 C indicates no coliform organisms present in the sample. Fluorescence under ultraviolet light is specific for the presence of Escherichia coli. Organisms Characteristics E. coli ATCC Growth E. aerogenes ATCC Growth E. faecalis ATCC Inhibited Organisms Coloring E. coli ATCC Yellow, fluorescence E. aerogenes ATCC Yellow, no fluorescence E. faecalis ATCC Red, no fluorescence Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, incubated at 35 C for 48 hours. Checked for fluorescence at 366 nm. Negative control: Sterile water incubated at 35º C for 48 hours. Sterility test: 14 days plated sterility test. ColiCheck with MUG: Left vial is inoculated with Escherichia coli ATCC 25922, right vial is control. Per liter of water and adjusted to ph 6.8 ± 0.2 Beef extract Pancreatic Digest of Gelatin Lactose Pancreatic Digest of Casein Dipotassium phosphate Monopotassium phosphate Sodium chloride Sodium lauryl sulfate Bromocresol purple MUG 3.0 g 5.0 g 7.5 g 10.0 g g g 2.5 g 50 mg 8.5 mg 125 mg Product Description Qty/Pkg Order No ColiCheck with MUG P/A test kit with sample bottles ColiCheck with MUG P/A test

17 Swabs Coliform SwabCheck, Hygiene SwabCheck and Listeria SwabCheck Products Coliform SwabCheck Coliform SwabCheck Escherichia coli and Coliforms are traditionally used as indicator organisms for fecal contamination in water and other environmental samples. Detection of these organisms usually points to poor hygiene at some stage in the production process or pollution of water at source. The presence of coliforms is indicated by a color change from red to yellow. The more rapid the color change the higher the level of coliform bacteria. Coliform SwabCheck. Hygiene SwabCheck Hygiene SwabCheck Easy to use: The Hygiene SwabCheck shows an obvious color change from red to yellow. The time taken for this change is an indication of the level of contamination. This should be used in conjunction with known specification levels of your process/product. Rapid screening hygiene test is a same day test which will detect gross bacterial and fungal contamination of work surfaces, equipment machinery or other sampling sites. Hygiene SwabCheck. Listeria SwabCheck Listeria SwabCheck, Listeria Isolation SwabCheck is designed to be used alongside traditional selective methods to improve the quality system and minimize the risk of Listeria contamination. This simple to use diagnostic test can be applied anywhere in the environment and on foodstuffs where the presence of Listeria species would be critical. Listeria species and specifically Listeria monocytogenes are rapidly becoming the most important pathogen in the food industry; regulatory bodies from around the world are insisting that all food products are Listeria free. Listeria Isolation SwabCheck works on an enhanced Esculin media formulation. The hydrolysis of esculin gives a distinctive black/brown precipitate. Inhibitors and antibiotics are present in the media which will inhibit the growth of non-listeria species. Listeria SwabCheck. Product Description Qty/Pkg Order No Coliform SwabCheck Units ready to use Hygiene SwabCheck Units ready to use Listeria SwabCheck Units ready to use

18 Products Swabs SwabCheck Escherichia coli SwabCheck Escherichia coli Used for the detection of Escherichia coli on surfaces. The presence of Escherichia coli is detected by the appearance of fluorescence throughout the tube. Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, hours at 35 37º C. Negative control: Enterobacter aerogenes ATCC 13048, hours at 35 37º C. Growth but not fluorenscence. Sterility: 7 days plated sterility test. SwabCheck Escherichia coli. Per liter of water adjusted to ph 6.9 ± 0.2 Description: The presence of fluorescence using a long-wave UV light source confirms the presnce of Escherichia coli, and any further confirmation is not required. MUG detects anaerogenic strain which may not be detected in the conventional procedure. Lactose is a source of energy. Casein peptone provides additional nutrients. The mixture of bile salts is inhibiting for gram-positive bacteria, particularly bacilli and fecal streptococci. The substrate 4-methylumbelliferyl-β- D-glucuronide is hydrolyzed by an enzyme, β-glucuronidase, possessed by most Escherichia coli and a few strains of Salmonella, Shigella and Yersinia, to produce a fluorescent end product, 4-methylumbelliferone. Pancreatic Digest of Casein Lactose Bile Salts Mixture Dipotassium Phosphate Monopotassium Phosphate Sodium Chloride 4-methylumbelliferyl-β- D-glucuronide 20.0 g 5.0 g 1.5 g 4.0 g 1.5 g 5.0 g 50 mg Product Description Qty/Pkg Order No SwabCheck Escherichia coli 4 ml ml

19 Total Count Swab Kit Used for the non-selective development and enumeration of all aerobic bacteria on surfaces in accordance with HACCP. The kit includes the swabs and culture medium, packaged with a membrane device, providing a quantitative result. Description: All bacteria develop on TGE media and produce a range of different colored and sized colonies. It is not possible using TGE to presumptively identify any bacteria. Identification can only be undertaken using traditional microbiology techniques following initial colony development. Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, hours at 35º C. Negative control: Not undertaken. Sterility: 7 days plated sterility test. Total Count Swab Kit. Per liter of water and adjusted to ph 7.0 ± 0.2 Pancreatic Digest of Casein Yeast extract Dextrose 10.0 g 5.0 g 2.0 g Yeast & Mold Swab Kit Used for the enumeration of yeast and molds on surfaces in accordance with HACCP. The kit includes the swabs and culture medium, packaged with a membrane device, providing a quantitative result. Description: M-Green yeast and mold is an improved modification of the liquid medium, m-yeast and Mold Broth and was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low ph, which inhibits bacterial growth. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the ph which aids in the inhibition of bacterial growth, but also produces an acid reaction which causes residual bromocresol green to change to yellow. Green opaque colonies against a yellow background are indicative of the growth of yeasts. Mold colonies are green and filamentous. Quality Control and recommended incubation conditions: Positive control: Candida albicans ATCC 10231, 48 hours at 25 30º C. Negative control: Not undertaken. Sterility: 7 days plated sterility test. Yeast & Mold Swab Kit. Per liter of water adjusted to ph 4.6 ± 0.2 Dipeptone Yeast extract Dextrose Magnesium sulfate Potassium phosphate Drastase Thiamine Bromocresol green 10.0 g 9.0 g 50.0 g 2.1 g 2.0 g 50 mg 50 mg 26 mg Product Description Qty/Pkg Order No Total Count Swab Kit pack Yeast & Mold Swab Kit pack

20 Products Swabs Transwab and Polywipe Sponge Transwab Transwab is a unique transport system for the safe and efficient transport of aerobic and anaerobic bacteria. Transwab is a transport system which is suited for a safe and efficient transport of aerobe and anaerobe microorganisms. Transwab contains ready to use transport swab with medium also for both aerobes and anaerobes. Transwab is available with Aimes and Aimes Charcoal medium. Application Aimes Medium Is reported to have a number of advantages over Stuart s, in particular Aimes uses an inorganic phosphate buffer. Glycerophosphate buffer in Stuart s can result in overgrowing by coliforms and gram-negative rods. Each batch of Transwabs are tested against a panel of organisms including aerobes, facultative anaerobes, true anaerobes and fastidious organisms: Low Bio burden Unique safety Bell Cap giving maximum protection to the user and specimen Transwab ensures the viability of organisms during transport Easy and safe to use Quality assurance guaranteed Large volume of oxygen reduced medium Tamper evident seal Group A Streptococcus Clostridium perfringens Escherichia coli Staphylococcus aureus Pseudomonas aeruginosa Amies Transwab and Amies Charcoal Transwab. Polywipe Sponge Used for the recovery of microorganisms from a surface. Description: Polywipe sponge is a specifically selected blue sponge that is premoistened with neutralizing buffer to neutralize the effects of surface disinfectants. The sponge material is selected to be free of the preservatives found in commercially available spon- ges which can inhibit microorganism growth. Polywipe sponges are biocide free and tested for zero toxicity to microorganisms. Each sponge is individually wrapped in a peel pouch and gamma irradiated to ensure sterility. Each box of 50 sponges are packed with 50 sterile gloves to allow aseptic handling and 50 sterile sampling bags to allow the sponge to be hydrated Polywipe Sponge (Ready to use). Product Description Qty/Pkg Order No Transwab Aimes Medium Aimes Charcoal Medium Polywipe Sponge In 110 ml container Ready to use, single packed

21 Colony Counting and Documentation Systems MBS Counter Products MBS Counter Fully equipped system for universal analysis of microcolonies for all conventional petri dish and agar plate formats down to colony size of < 0.1 mm. The new generation counter of Schleicher & Schuell MicroScience fits perfectly in the modern laboratory with its LIMS compatible software. The MBS-Counter is a rapid, effective and safe tool for documentation and analysis of microbiological samples. Every detail of the system is designed to meet high performance applications in the analysis of colonies especially in routine labs. The image processing unit takes pictures of the petri dishes with a high resolution camera. Lighting of the samples is performed with a unique light plate which gives reproducible conditions every time and eliminates light scattering on shiny surfaces through the cloudy day adjustment. The system is capable of analysing all petri dish formats and differentiates colony colors and morphology. Colony clusters are recognized as individual colonies. The software operates in a fuzzy logic mode, thus time consuming analysing operations are performed in a background while up to 500 sample pictures/hour can be taken by the operator. The documentation for processed data is managed in the form of a data base: Number of colonies, comments, results of sampling points etc. are available at any time for further reports or trend analysis. The MBS Counter is a complete system consisting of a computer with installed software, monitor and the image processing unit. 92

22 Products Colony Counting and Documentation Systems MBS Counter Workflow 1 1. Sample identification. 2. Put sample into the image processing unit. 3. After using the enter-key the system confirms the successful picture input. 4. Analysis of single samples independent of pictures are taken by the MBS Counter. Advantages Easy to use LIMScompliant software Automatically evaluation: time saving up to 70% Exact Colony counting Documentation according to ISO Can be used for all petri dish formats Within one year

23 Colony Counting and Documentation Systems MBS-Counter Products Technical data: Staph. aureus, Standard TTC, green* PC: Operating System: Microsoft Windows NT 2000 Monitor: 19 Monitor VGA: AGP Grafik NET: LAN Intel 10/100 Processor: Pentium 4/1.4 GHz/256 MB FCPGA RAM: 1 x 256 MB SDRAM PC 133 ECC CD-ROM: 40-fold Ultra Hard Drive: 36 GB Staph. aureus, Caso AP MBS Counter Standard: MBS Counter Digital: 10 x optical zoom lens 4 x digital zoom 1/4 IT CCD-Sensor Super HAD-Technology ( pixel) CCD ~4.0 - millions of pixel 4 x zoom, f = 8-32 mm Staph. aureus, Standard TTC, white* Dimension of equipment box: Ø 45 cm, hight 47 cm Staph. aureus, Standard TTC, black* * Pictures are taken after using membrane filtration method. Product Description Qty/Pkg Order-No MBS Counter Standard Fully equipped system with Standard Resolution System 1 Please contact our Scientific Service Center MBS Counter Digital Fully equipped system with High Resolution System 1 Please contact our Scientific Service Center 94

24 Products Colony Counting and Documentation Systems Counter Counter Documentation system for the evaluation of microcolonies on membrane filters with size < 0.5 mm The Counter evaluation system from Schleicher & Schuell MicroScience automatically counts colonies on membrane filter. A PC coupled with a camera then documents the result as an image and stores it together with the corresponding data in a database. All results can be accessed at any time and compared with one another. The advantages: trend analyses are considerably easier; records containing process standards and stability more meaningful. And all quality assurance data remains transparent, even for the certifying authorities. The system, which uses a Windows operating system, is designed for routine operation and, above all, has been optimized for rapid measurement by constantly repeating sample matrices. Two security modules are responsible for data security: a hardware key (dongle) activates the program and a password is required to access the system. This means that all the data is protected against third-party interference and manipulation Counter for analyzing membrane filter samples Counter is an ideal system used in microbiological laboratories for the accurate counting of routine membrane filter samples. Technical data for the camera system: Precision: > 98% Resolution: 752 x 582 Max. enlargement: approx. 30 x Average document size: kb Delivery range: Video microscope, stand, framegrabber, connecting cable, installation-cd, operating instructions. Advantages Easy to use due to userfriendly operation with Windows and pre-set parameters Automatic colony counting Precise colony counts ISO-compliant documentation Product Description Qty/Pkg Order-No Counter Delivery range as described above Accessories Neon ring-light