Product Datasheet. CD31/PECAM-1 Antibody NB Unit Size: 0.1 ml. Store at 4C. Do not freeze. Reviews: 4 Publications: 18

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1 Product Datasheet CD31/PECAM-1 Antibody NB Unit Size: 0.1 ml Store at 4C. Do not freeze. Reviews: 4 Publications: 18 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 4/11/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB CD31/PECAM-1 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.1 ml 1.0 mg/ml Store at 4C. Do not freeze. Polyclonal 0.09% Sodium Azide IgG Immunogen affinity purified TBS and 0.1% BSA Page 1 of 5 v.20.1 Updated 4/11/2018 Product Description Host Rabbit Gene ID 5175 Gene Symbol Species PECAM1 Human, Mouse, Rat, Porcine Reactivity Notes Mouse reactivity reported in scientific literature (PMID: ) Immunogen Product Application Details Applications The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 738) of human CD31 using the numbering given in entry NP_ (GeneID 5175). Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunocytochemistry Recommended Dilutions Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunohistochemistry- Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunocytochemistry 1:100 Application Notes Images For IHC-P: Epitope exposure is recommended, with citrate buffer will enhance staining. In some cases, the antibody may be diluted further than indicated. Immunohistochemistry-Frozen and Western Blot were reported in scientific literature. ICC/IF reactivity reported in scientific literature (PMID: ). Immunohistochemistry: CD31/PECAM-1 Antibody [NB ] - Matrix-HA supplemented with strontium was implanted subcutaneously in mice, where immunochemistry analysis and histology occurred of the newly formed tissues. (C) Within each matrix: Matrix-HA, Matrix-8Sr-HA and Matrix-50Sr-HA, were indicated by immunostaining of CD31 for the newly formed tissues. (D) Number of vessels within each tissue was quantified using NDP view software. Unit is square mm. Immunostaining analysis of slides occurred for 2 samples per condition and 3 sections were analyzed per sample and per group of matrix. Citation: Ehret C, Aid-Launais R, Sagardoy T, Siadous R, Bareille R, Rey S, et al. (2017) Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation. PLoS ONE 12(9): e

3 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB ] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-cd31/pecam (NB ) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG-Hilyte PlusTM 555 Page 2 of 5 v.20.1 Updated 4/11/2018 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB ] - Human lung adenocarcinoma. Used at a dilution of 1:100 with citrate epitope retrieval ph6.0. Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NB ] - Analysis in pig SC cells using anti-cd31 antibody (red). Blue color showing nucleus labeling. Image from verified customer review. Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB ] - Anaysis in rat muscle blood vessels using anti-cd31 antibody. Image from verified customer review.

4 Immunocytochemistry: CD31/PECAM-1 Antibody [NB ] - Imaging of HUVEC monolayer on glass substrate. Fixation 4% PFA Blocking Goat Serum Dilution 1:100. Incubation RT. This image was submitted via customer Review. Page 3 of 5 v.20.1 Updated 4/11/2018 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB ] - Immunostaining of CD31/PECAM-1 in Rat Liver sample. This image was submitted by customer review. Immunohistochemistry: CD31/PECAM-1 Antibody [NB ] - Section of human colon adenocarcinoma.

5 Publications Ehret C, Aid-Launais R, Sagardoy T et al. Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation PLoS ONE 2017 Sep 14 [PMID: ] (IHC-P, Human) Mohamed R, Sharma I, Ibrahim AS et al. Hyperhomocysteinemia Alters Retinal Endothelial Cells Barrier Function and Angiogenic Potential via Activation of Oxidative Stress Sci Rep 2017 Sep 20 [PMID: ] (ICC/IF, Human) Meyers MW, Rink JS, Jiang Q et al. Systemically administered collagen-targeted gold nanoparticles bind to arterial injury following vascular interventions. Physiol Rep Feb 01 [PMID: ] (ICC/IF) Oliveira H, Catros S, Castano O et al. The proangiogenic potential of a novel calcium releasing composite biomaterial: orthotopic in vivo evaluation. Acta Biomater Feb 24 [PMID: ] (WB, Rat) Luo L, Chen B, Huang Y et al. Cardioprotective activity of placental growth factor combined with oral supplementation of l-arginine in a rat model of acute myocardial infarction. Drug Des Devel Ther. Nov :00AM [PMID: ] (Rat) Modulevsky DJ, Cuerrier CM, Pelling AE. Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials. PLoS One 2016 Jun 21 [PMID: ] (ICC/IF) Moon J, Kim Jh, Choi D et al. Correlation of quantitative dynamic contrast-enhanced MRI with microvascular density in necrotic, partial necrotic, and viable liver tumors in a rabbit model. J Appl Clin Med Phys Sep :00AM [PMID: ] (IHC) De Paoli F, Eeckhoute J, Copin C et al. The neuron-derived orphan receptor 1 (NOR1) is induced upon human alternative macrophage polarization and stimulates the expression of markers of the M2 phenotype Atherosclerosis 2015 [PMID: ] (IHC-Fr, Human) Bories G, Colin S, Vanhoutte J et al. Liver X Receptor (LXR) Activation Stimulates Iron Export in Human Alternative Macrophages. Circ Res Sep 13 [PMID: ] (IHC, Human) Abele-Ohl S, Leis M, Wollin M et al. Human cytomegalovirus infection leads to elevated levels of transplant arteriosclerosis in a humanized mouse aortic xenograft model. Am J Transplant 2012 Jul [PMID: ] Schatton T, Murphy GF, Frank NY et al. Identification of cells initiating human melanomas. Nature 2008 Jan [PMID: ] Wang F, Li SS, Segersvard R et al. Hypoxia inducible factor-1 mediates effects of insulin on pancreatic cancer cells and disturbs host energy homeostasis. Am J Pathol 2007 Feb [PMID: ] More publications at Page 4 of 5 v.20.1 Updated 4/11/2018

6 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF008 NB7156 NBP NBP PEP Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control CD31/PECAM-1 Recombinant Protein Antigen Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: