University of Guelph. Supply Center Product List

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1 University of Guelph Supply Center Product List

2 Supply Center Location 488 GORDON ST SCI COMPLX BLDG 140 IVGN SPLY To find out more about your Supply Center, request product additions to the Supply Center, or find out more about Thermo Fisher Scientific products and services, contact: Allison MacMullin Account Manager (905) Technical Support Hours: 8.00am-8.00pm EST Phone: Need a product added to your Supply Center? Please contact us: SupplyCenterProductRequests@lifetech.com Supply Center Customer Service North America Phone: supplycenters@lifetech.com For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

3 Product Name Size Storage Cat. No. Price* Blasticidin S HCl, powder 50 mg R21001 $ Zeocin Selection Reagent 8 x 1.25 ml R25001 $ Hygromycin B (50 mg/ml) 20 ml $ Penicillin-Streptomycin (10,000 U/mL) $ Antibiotic-Antimycotic (100X) $ PBS, ph $ 9.48 DPBS, no calcium, no magnesium $ 9.44 PBS (10X), ph $ DMEM/F-12, HEPES $ 8.87 DMEM, high glucose $ 9.40 Trypsin-EDTA (0.5%), no phenol red $ Trypsin-EDTA (0.25%), phenol red $ Trypsin-EDTA (0.05%), phenol red $ ml $ Fetal Bovine Serum, qualified, USDA-approved regions $ Fetal Bovine Serum, qualified, Canada origin $ Lipofectamine 2000 Transfection Reagent 1.5 ml $ Lipofectin Transfection Reagent 1 ml $ Lipofectamine 3000 Transfection Reagent 1.5 ml L $ High-Capacity cdna Reverse Transcription Kit 200 reactions $ ThermoScript -PCR System for First-Strand cdna Synthesis 25 reactions $ SuperScript VILO cdna Synthesis Kit 50 x 20 µl reactions $ SuperScript First-Strand Synthesis System for -PCR 50 reactions $ SuperScript III First-Strand Synthesis System 50 reactions $ Cell Culture & Transfection Reagents Antibiotics & Antimycotics Balanced Salt Solutions Basal Media Cell Dissociation Reagents Cryopreservation Media Recovery Cell Culture Freezing Medium Serum Transfection Reagents Kits & Assays cdna Synthesis, Library, & Cloning Kits

4 Product Name Size Storage Cat. No. Price* PureLink HiPure Plasmid Midiprep Kit 25 preps K $ PureLink Quick Plasmid Miniprep Kit 50 preps K $ PureLink Quick Plasmid Miniprep Kit 250 preps K $ PureLink Quick Gel Extraction Kit 50 preps K $ PureLink PCR Purification Kit 50 preps K $ TRIzol Reagent 200 ml $ TRIzol Reagent $ PureLink RNA Mini Kit 50 preps A $ PowerUp SYBR Green Master Mix 5 ml A25742 $ Power SYBR Green PCR Master Mix 1 x 5 ml $ Fast SYBR Green Master Mix 1 x 5 ml $ Platinum SYBR Green qpcr SuperMix-UDG 100 reactions $ Platinum SYBR Green qpcr SuperMix-UDG 500 reactions $ g $ x 1 ml $ RNAlater Stabilization Solution AM7021 $ RNaseZap RNase Decontamination Solution 250 ml AM9780 $ DNAZap PCR DNA Degradation Solutions 250 ml AM9890 $ BlueJuice Gel Loading Buffer (10X) 3 x 1 ml $ UltraPure Agarose 100 g $ UltraPure Agarose 500 g $ UltraPure Phenol 500 g $ UltraPure Buffer-Saturated Phenol $ reactions $ $ $ mg $ DNA RNA Purification Kits & Reagents Real-Time PCR & Endpoint -PCR Kits Lab Reagents & Chemicals Denaturing Agents UltraPure Formamide DNA RNA Reagents UltraPure Salmon Sperm DNA Solution DNA RNA Storage & Nuclease Control Reagents Electrophoresis Reagents & Buffers General Chemicals Molecular Biology Enzyme Buffers PCRX Enhancer System Water UltraPure DNase/RNase-Free Distilled Water Labeling & Detection Products Cell Stains Trypan Blue Solution, 0.4% Enzyme Substrates X-Gal

5 Product Name Size Storage Cat. No. Price* SimplyBlue SafeStain 1L MIX LC6060 $ SYBR Safe DNA Gel Stain 400 µl S33102 $ Oligo(dT)12-18 Primer 25 µg $ Random Primers 9 units $ dntp Set (100 mm) 4 x 250 µl $ dntp Mix (10 mm ea) 100 µl $ RNaseOUT Recombinant Ribonuclease Inhibitor 5,000 units $ Ribonuclease Inhibitor, Cloned 1,000 units $ Platinum Taq DNA Polymerase 120 reactions $ Platinum Taq DNA Polymerase 600 reactions $ Platinum Taq DNA Polymerase High Fidelity 100 reactions $ Platinum PCR SuperMix 100 reactions $ Platinum Pfx DNA Polymerase 100 reactions $ Platinum Taq Green Hot Start DNA Polymerase 120 reactions $ BamH I 2,000 units $ EcoRI 5,000 units $ Hind III 5,000 units $ Pst I 3,000 units $ T4 DNA Ligase (1 U/µL) 500 units $ Xho I (PaeR7I) 2500 units $ Dpn I 100 units $ Cla I 500 units $ Spe I 200 units $ Nhe I 300 units $ DNA Polymerase I, Large (Klenow) Fragment 100 units $ Ribonuclease H 30 units $ Taq DNA Polymerase, native 100 units $ SuperScript II Reverse Transcriptase 10,000 units $ DNase I, Amplification Grade 100 units $ SuperScript III Reverse Transcriptase 10,000 units $ Proteinase K Powder ( 40 U/mgP) 100 mg $ Proteinase K Powder ( 20 U/mg) 1g $ M-MLV Reverse Transcriptase (200 U/µL) 40,000 units $ AmpliTaq Gold DNA Polymerase with Buffer II and MgCl2 250 units N $ Gel & Membrane Stains Oligos, Primers, Probes & Nucleotides DNA Primers & Oligos Nucleotides Proteins, Enzymes, & Peptides Enzyme Inhibitors PCR & Cloning Enzymes

6 Product Name Size Storage Cat. No. Price* Low DNA Mass Ladder 50 applications $ TrackIt 1 Kb Plus DNA Ladder 100 applications $ Kb Plus DNA Ladder 250 µg $ ΦX174 RF DNA/Hae III Fragments 40 µg $ bp DNA Ladder 50 µg $ bp DNA Ladder 250 µg $ MagicMark XP Western Protein Standard 250 µl LC5602 $ Novex Sharp Pre-stained Protein Standard 2 x 250 µl LC5800 $ SeeBlue Plus2 Pre-stained Protein Standard 500 µl LC5925 $ BenchMark Protein Ladder 2 x 250 µl $ BenchMark Pre-stained Protein Ladder 2 x 250 µl $ Standards, Ladders & Controls DNA RNA Ladders Protein Standards & Ladders

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