Academic Qualifications:

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1 CURRICULUM VITAE Dr. RANJAN SINGH Dr. Ranjan Singh Date of Birth: Mobile: ID: Academic Qualifications: Ph.D. in Microbiology from Department of Microbiology, Dr. R. M. L. Avadh University, Faizabad, U.P., India Master of Science- Microbiology from Department of Biological Sciences, R. D. University, Jabalpur, M.P., India Passed in I Division. Bachelor of Science- Chemistry, Zoology, Botany from University of Lucknow, Lucknow, U.P., India Passed in II Division. Intermediate from St. Francis College, Lucknow, U.P., India ISCE, New Delhi. Passed in I Division. High School from St. Francis College, Lucknow, U.P., India ICSE, New Delhi. Passed in I Division. Research Experience: Worked in a Project entitled Isolation and Characterization of Aeromonas Sp. from Channa punctatus (Bloch), a fresh water fish, exposed to Lead at Industrial Toxicology Research Centre, Lucknow, C.S.I.R. New Delhi, Ministry of HRD, Govt. of India, under the guidance of Dr. K.G. Dubey (Scientist E-II & Assistant Director, Aquatic Toxicology Lab). Duration of project work 6 months. In this particular work 1

2 the effect of Aeromonas species was seen on some vital organs of fresh water fish which has been exposed to lead found in river water. Environmental Microbiological Work. The work was published by Lambert Academic Publishing House, Germany in form of monograph entitled Characterization of Aeromonas in ISBN Worked as Project Fellow, University Grants Commission, New Delhi, (Ministry of HRD, Govt. of India.) sponsored project in Department of Microbiology, Dr. R. M. L. Avadh University, Faizabad, (U.P.). Duration of project 3 years. In this work a fungus Aureobasidium pullulans was isolated which produced an industrially important polysaccharide Pullulan. The fungus was optimized at fermentor level to produce the polysaccharide. Further the strain was improved for high production of pullulan at fermentor level. In this investigation a novel fungus Eurotium chevalieri has been isolated and optimized at various physico-chemical and nutritional parameters, a novel aspect, which has been reported first time for higher pullulan which is at par to the production of pullulan with A. pullulans. Specialization in Bioprocess Technology (Industrial Microbiology). This work was published by Lambert Academic Publishing House, Germany in form of monograph entitled Production of Pullulan in ISBN Amity Institute of Microbial Biotechnology, Amity University Noida Campus, carried out research work on mass production of industrially important polysaccharide at laboratory level as well as biodegradation of organic waste especially kitchen waste (Environmental Microbiology). Duration of work 1.2 years. Bajaj Hindusthan Limited, worked as Production Officer in Distillery. Involved in laboratory work as maintenance of yeast strain and yeast propagation in laboratory as well its strain improvement for higher production of alcohol. Beside this looked after the production of alcohol at large scale fermentor level and mass production of yeast. Duration of work 1.5 years. Currently working on production of pullulan at laboratory level for mass production of this important polysaccharide as well as strain improvement of the fungus for increased production of polysaccharide. Working Experience: Worked as Teaching Assistant in Department of Microbiology and Department of Biotechnology, Dr. R. M. L. Avadh University, Faizabad, U. P. (Teaching experience of 2.5 years in Avadh University). Took theory and practical classes at Post Graduate (M. Sc) level. Worked as Assistant Professor in Amity Institute of Microbial Biotechnology (AIMB), Amity University, Noida Campus, U. P. Take theory and practical classes at Graduate (B. Sc. / B. Tech) and Post Graduate (M. Sc. / M. Tech) level. 2

3 Worked as Officer Production (Distillery) in Bajaj Hindusthan Limited, Kheri, UP. Currently Working as Assistant Professor in Department of Microbiology, St. Aloysius College (Autonomous), Jabalpur, Affiliated to R.D. University, Jabalpur. Take theory and practical classes of Microbiology and Biotechnology students at Graduate (B.Sc.) and Post Graduate (M.Sc.) level and also involved in research work carried out in the department. Also take Environmental Science Classes. Working Abilities: Fermentation Process/Bioprocess Technology. Working experience on fermentor at laboratory and industrial level. Working experience on various environmental aspects. Working experience on various microbiological and biotechnological aspects. Subjects Taught at PG and UG level: Bioprocess Technology/Fermentation Technology Industrial Microbiology Virology Medical Microbiology Environmental Sciences Microbial Genetics Techniques Know: Fermentor Various Microbiological and Biotechnological Techniques. 3

4 Brief summary of the work done during Ph.D. research: Thesis Title: Improvement of Aureobasidium pullulans strain and its immobilization for increased production of pullulan in batch and fed-batch fermentation. Since pullulan is an important polysaccharide with 300 patents of its applications in various industries, one of the most promising use for the production of biodegradable plastics. Till date only mesophilic strains of A. pullulans has been exploited for production of this polysaccharide worldwide while the thermotolerant strain is the need of hour. In this context we have successfully isolated a thermotolerant higher pullulan producing strain from natural ecosystem. This fungus has been critically evaluated at different physico-chemical and nutritional parameters for pullulan production in flask, stirred and non-stirred fermentation condition in a fermentor of 7 liter capacity. A suitable mutant has also been successfully developed for higher pullulan without disturbing thermotolerant nature of wild strain. The review of literature clearly indicates that less attention has been paid towards development of bioreactor in order to achieve continuous production. Therefore, a novel fermentation model having immobilized microbial cells of A. pullulans has been successfully developed in order to achieve continuous production of pullulan. This study has been compared in several designs with all correction factors like backflow of air, viscosity, dilution rate and other factors to achieve the constant production of pullulan. In this investigation a novel fungus Eurotium chevalieri has been isolated and optimized at various physico-chemical and nutritional parameters, a novel aspect, which has been reported first time for higher pullulan which is at par to the production of pullulan with A. pullulans. Academic International Publications: R. Gaur and Ranjan Singh (2010). Optimization of physico-chemical and nutritional parameters for pullulan production by a mutant of thermotolerant Aureobasidium pullulans in fed batch fermentation process. African Journal of Biotechnology. 9 (43), R. Gaur, Ranjan Singh, S. Tiwari, S. K. Yadav, N. S. Darmwal (2010). Optimization of physico-chemical and nutritional parameters for a novel pullulan producing fungus, Eurotium chevalieri. Journal of Applied Microbiology. 109: R. Gaur, Ranjan Singh, M. Gupta, M. K. Gaur (2010). Aureobasidium pullulans, an economically important polymorphic yeast with special reference to pullulan. African Journal of Biotechnology. 9 (47),

5 F. Jamal, T. Qidwai, P. K. Pandey, Ranjan Singh, S. Singh (2011). Azo and Anthraquinone dye decolourization in relation to its molecular structure using soluble Trichosanthes dioica peroxidase supplemented with redox mediator. Catalysis Communication. 12: S. Tiwari, R. Gaur, Ranjan Singh (2012). Decolorization of a recalcitrant organic compound (Melanoidin) by a novel thermotolerant yeast, Candida tropicalis RG-9. BMC Biotechnology :30. Ranjan Singh, Rajeeva Gaur, Soni Tiwari, M.K. Gaur (2012). Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process. Brazilian Journal of Microbiology. 43:3. pp Ranjan Singh, R. Gaur, F. Jamal, M. K. Gaur (2013). A novel model of fermentor system for continuous production of pullulan by immobilized cells of Aureobasidium pullulans. African Journal of Biotechnology. 10(48), pp Santosh Kumar Yadav, Deepali Bisht, Ranjan Singh, Rajeeva Gaur, Nandan Singh Darmwal (2013). Development of bioprocess for improved production of Alkaline Protease by mutant strain of Aspergillus flavus in solid state fermentation using agricultural waste. The Internet Journal of Microbiology. Vol. 12 No. 1 Ranjan Singh, Rajeeva Gaur, Manogya Kumar Gaur, Prabhash Kumar Pandey, Farrukh Jamal (2015). Antimicrobial activity of a thermotolerant Aureobasidium pullulans strainisolated from Faizabad region of Uttar Pradesh in India. International Journal of Current Microbiology and Applied Science. 4(3) Ranjan Singh, Rajeeva Gaur, Pritha Biswas, Shikha Bansal (2015). Aureobasidium pullulans, An economically important black yeast. Journal of Basic and Applied Mycology. 11 (1 and 2) Ranjan Singh, Rajeeva Gaur, Shikha Bansal, Pritha Biswas, Prabhash Kumar Pandey, Farrukh Jamal, Soni Tiwari and Manogya K. Gaur (2015). Aureobasidium pullulans. An industrially important producing black yeast. International Journal of Current Microbiology and Applied Science. 4(10) International Book Chapters: Ranjan Singh, Rajeeva Gaur, R.R. Pandey (2012). Biology, distribution and utility of Aureobasidium pullulans. In Microbial Applications edited by Rajeeva Gaur, Sudhir Malhotra and R.R. Pandey. Published by I. K. International Publishing House, New Delhi, India. Vijay Kumar Gupta, Aarti Gupta, Santosh Kumar Yadav, Ranjan Singh, Rajeeva Gaur (2012). Bacterial Protease Inhibitors. In Microbial Applications edited by Rajeeva 5

6 Gaur, Sudhir Malhotra and R.R. Pandey. Published by I. K. International Publishing House, New Delhi, India. Ashish Kumar Asthana, Rajeeva Gaur, Ranjan Singh (2012). Microbial Degradation of Sulfide in Aerobic and Anaerobic Conditions. In Microbial Applications edited by Rajeeva Gaur, Sudhir Malhotra and R.R. Pandey. Published by I. K. International Publishing House, New Delhi, India. Rajeeva Gaur, Soni Tiwari, Manogya Kumar Gaur, Ranjan Singh (2013). Potentials of Methanogens in Natural Ecosystem. In Recent Advances in Microbiology Volume II edited by S.P. Tiwari, Rajesh Sharma and Rajeeva Gaur. Published by Nova Science Publishers Inc., New York, USA. International Books Published: Characterization of Aeromonas (2011). Published by LAP Lambert Academic Publishing House, Germany. ISBN Production of Pullulan (2011). Published by LAP Lambert Academic Publishing House, Germany. ISBN Microbial Toxins (2011). Published by LAP Lambert Academic Publishing House, Germany. ISBN Aureobasidium pullulans (2011). Published by LAP Lambert Academic Publishing House, Germany. ISBN Pullulan, The Magical Polysaccharide. (2015). Published by LAP Lambert Academic Publishing House, Germany. ISBN Seminars Delivered during Post Graduation: Chemical Kinetics. Bacteriophage and its application to Human Beings. Bioluminescence and its practical application to Human Beings. Plant Pathogens and their renewal (Integrated Pest management). 6

7 National/International Workshops/ Trainings/ Conferences Attended: Attended a three days workshop on Applied Techniques in Biology held at St. Aloysius College, Jablpur, M. P. from 5-7 Febuary Presented research paper entitled Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process at 100 th Indian Science Congress held at Kolkata from 3-7 January 2013 and awarded Young Scientist Award in Plant Science Section Attended two days seminar on Capacity Building on Environment Management in Mines held at ACE Caldery Refratory Work, Katni, M. P. on May, Organized by Intenatinal Institute of Waste Management (IIWM), Bhopal, M. P. Attended National Seminar on Environmental Pollution held at Amity University Noida on 11 May, Attended a National Seminar on HIV and AIDS held at Amity University, Noida, on March, Attended two days seminar on Capacity Building on Waste Management in Industrial Sector held at M/S Godrej Consumer Products Ltd., Bhind, M. P. on January, Organized by Intenatinal Institute of Waste Management (IIWM), Bhopal, M. P. Attended a National Seminar on Climate Change and its Impact on Biological Communities at Dr. R. M. L. Avadh University, Faizabad on February, Attended National Workshop and Training on Fungal Identification and Characterization held at R. D. University, Jabalpur from 6-11 September Presented Research Paper at Micro-tech 2009 entitled Optimization of physico-chemical and nutritional parameters for a novel pullulan producing fungus Eurotium chevalieri held at R.D. University, Jabalpur on 11 September 2010 and awarded the Junior Scientist Award. Attended National Seminar on Current Advances in Biosciences: Application in Health, Environment and Agriculture and presented Research Paper entitled Optimization of physico-chemical and nutritional parameters for pullulan production by a mutant of thermotolerant Aureobasidium pullulans in fed batch fermentation process held at Hitkarini Women College, Jabalpur, on November Attended 50 th A.M.I. Conference at N.C.L., Pune from December Fifteen days Industrial Training in Fermentation and Distilleries Technology at Mohan Meakin Ltd, Lucknow from February Attended a Workshop on Biotechnology sponsored by Ministry of H.R.D., Government of India, held at R.D. University, Jabalpur from July Attended Workshop and Training on Spirulina cultivation held at R.D. University, Jabalpur from January Attended National Symposium and Micro-tech held at R.D. University, Jabalpur on 19 November Attended International Symposium and Micro-tech held at R.D. University, Jabalpur from November

8 Faculty Development Program Attended: Attended Faculty Development Program on Research Methodology held on December, 2011at Amity University Uttar Pradesh, Noida. Attended Faculty Development Program on Amity Academic Structure and System held on 4-5 July, 2012 at Amity University Uttar Pradesh, Noida. Achievements and Awards: Awarded the Young Scientist Award at 100 th Indian Science Congress in Plant Science Section held at Kolkata from 3-7 January Awarded by Indian Science Congress Association (ISCA) Kolkata. Awarded the Junior Scientist Award at Micro-tech Gold Medal in Oral Paper Presentation. Presented by Society for Basic and Applied Mycology (SBAM) at Microtech held at R.D. University, Jabalpur, on 11 September Dissertation Supervised: Supervised a six months dissertation work in Microbiology at P.G. level. Title of the work Optimization of physicochemical and nutritional parameters for production of Pullulan in non stirred flask type fermentation system. Summer Training for UG and PG Students: Trained students of UG and PG in ten days summer training on Microbial Tools and Techniques organized by the Department. Trained students in various techniques like Culture Making, Fermentation Technique, Slide and Cover Slip Technique and various other techniques related to microbiology. Life Memberships of various Academic Bodies: Indian Science Congress Association (I.S.C.A.) Association of Microbiologist of India (A.M.I.) Society for Basic and Applied Mycology (S.B.A.M.), R. D. University, Jabalpur. 8

9 Personal Details Date of Birth: Languages Known: Hindi, English Address for Correspondence: Dr. Ranjan Singh, S/O Mr. Madhuker Singh, C-17, Government Colony, Nirala Nagar, Lucknow , Uttar Pradesh, India. ID: Mobile Number: Declaration I hereby declare that the above-mentioned information is correct up to my knowledge and I bear the responsibility for the correctness of the above-mentioned particulars. (Dr. Ranjan Singh) C-17, Government Colony, Nirala Nagar, Lucknow , Uttar Pradesh, India. 9