Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood

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1 ก ก ก ก

2 Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood

3 ก miniaturized thermal biosensor flow-injection analysis ก (1 µl) ก ก ก mmol/l 1 µl ก ก flow-injection thermal biosensor ก ก ก

4 Conclusion Thermal biosensor flow injection analysis ก = 5 min 1 Enz. Column 100 plasma serum กก 10 Linear range = mmol/l glucose 1µL ก ก the thermal biosensor ก ก ก ref. method

5 : ก epinephrine White rot fungi

6 Conclusion ก epinephrine ก ก White-rot fungi ก (microbial biosensor) laccase ก ก ก epinephrine ก ก ก ก ก ก ก epinephrine ก 7

7 .. ก AtcharapornPonrin Areerat Srinuan Zajoncov, L.; Jlek, M.; Beranov, V.; Pe. BiosensBioelectron, 2004, 20,

8 ก ก activity ก H 2 O 2

9 Conclusions ก activity amperometric biosensor peroxide electrode flow injection analysis (FIA) reacting layer biosensor ก immobilisation enzyme glucose oxidase mutarotase ก (response times 35 s) sensitivity maltose Enzyme membrane 3 ( mutarotase) 2 ( mutarotase) biosensor mutarotase activity error FIA ก ก activity biosensor method 9

10 Amperometric biosensor for uric acid based on uricase-immobilized silk Fibroin membrane ก ก ก Yu-Qing Zhang, Wei-De Shen, Ren-Ao Gu, Jiang Zhu, Ren-Yu Xue,:Biotechnology Laboratory for Silkworm and Silk, School of Sericulture, Suzhou University, Suzhou , China Analytica Chimica Acta 369 (1998) 123±128 ก ก ก ก ก ก ก ก ก ก ก ก ก

11 Sensor fabrication and electrochemical apparatus ก ก ก peristaltic pump, home-made micro-cell, heat exchanger, oxygen electrode (Pt cathode and Ag anode) oxygen-permeable polyethylene film uricaseimmobilized fibroin membrane amplifier for the oxygen electrode ก buffer, 0.02 mol/l phosphate solution, ph 8.50; flow rate, 1.50ml/min; temperature, 32.0± C; polarizing voltage, V; sample volume per injection, 50 ml

12 Development of a high analytical Performance-xanthine biosensor based on layered double hydroxides modified-electrode electrode and investigation of the inhibitory effect by allopurinol D. Shana, Y. Wang, M. Zhu, H. Xue, S. Cosnier, C. Wang: Biosensors and Bioelectronics 24 (2009)

13 Construction of biosensor and enzyme immobilization

14 ก xanthine ก (XnOx) xanthine ก ก amperometry ก ก V ก layered double hydroxides immobilize enzyme ก ก ก ก ก ก xanthine ก ก XnOx allopurinol allopurinol ก ก ก ก ก ก

15 - XnOx/LDHs biosensor - Swelling property, High porosity, Thermal stability - Anionic exchange ability induced the highly developed analytical performance to xanthine. - Biosensor ก ก ก ก xanthine ก ก ก ก uric acid

16 Mustafa Kemal Sezgintürk, Tüge Göktuğ, Erhan Dinçkaya Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey

17 ก ก ก ก ก ก ก ก กก ก ก ก ก ก ก ก กก ก ก ก

18 To design a new elctrochemical biosensor for detection of T. harzianum species which can achieve high specificity and sensitivity, by detection of interaction of DNA probe. Different electrodes were developed as an aperometric transducer: 1. a bare gold electrode (AuE) with MB 2. a bare gold electrode (AuE) without MB 3. probe modified electrode (AuE/MPA/SAM) with MB 4. probe modified electrode (AuE/MPA/SAM) without MB

19 ก ก ก ก oxidases ก ก,, ก ก amperometric biosensor sensitivity ก ก ก biosensor ก

20 Results 1. Linear range mm 2. Sensitivity ก 1.22 µa/mm sensitivity ก ก 3. Correlation coefficient ก Detection limit ก 50 µm ก signal-to-noise ratio of 3 5. Repeatability ก glucose biosensor ก 5 mm ก กก 8 ก 6.07 µa 6. Relative standard deviation (R.S.D) ก 6.5%

21 Discussion 1. Woodceramic ก ก ก ก 2. TBO ก ก GOD woodceramic electrode 3. ก ก ก ก ก ก reduction TBO glucose electrode ก ก ก 4. ก oxidation ก ก ก Glucose oxidase Woodceramic ก ก gluconic ph 5. woodceramic electrode ก ก ก ก

22 Development of disposable amperometricsulfur dioxide biosensors based on screen printed electrodes J.P. Hart, A.K. Abass, D. Cowell, Biosensors & Bioelectronics 17 (2002) 389. ก ก ก ก ก ก Advanced Electroanalytical Chemistry

23 กก electrocatalytic reaction enzymatic reactions ก mediater ก ก ก ก ก ก ก ก ก

24 ก biosensor SO 2 S-type biosensor Electrolyte containing SOD and Cyt. c Polycarbonate membrane Ag/AgCl Carbon electrode PVC Substrate SO 2 B-type biosensor Carbon layer containing SOD and Cyt. c electrolyte

25 conclusion biosensor 24 ก biosensor ก biosensor 5 SO 2 10 ppm ±5% s-typebiosensor ก b-type biosensor b-biosensor ก 3 biosensor ก ก ก ก O 2 CO, H 2,S thiol

26 Analytical Chemistry Chiang Mai University Xu-Dong Wang, Ting-Yao Zhou, Xi Chen,Kwok-Yin Wong, Xiao-Ru Wang, An optical biosensor for the rapid determination of glucose in human serum, 129 (2008) ก

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28 ก optical glucose biosensor ก glucose oxidase immobilize ORMOSIL PVA biosensor ก response time detection limit sensitivity ก ก ก ก ก kinetic curve simulation method ก ก 1 ก 1 28