QSTAR XL Hybrid LC/MS/MS System. Extended Performance Plus Exceptional Flexibility. QSTAR XL. Hybrid LC/MS/MS System

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1 QSTAR XL Hybrid LC/MS/MS System Extended Performance Plus Exceptional Flexibility. QSTAR XL Hybrid LC/MS/MS System

2 Extended performance and flexibility for your most challenging analyses. Applied Biosystems/MDS SCIEX QSTAR XL LC/MS/MS System gives you industry-leading sensitivity, mass accuracy, and mass stability to deliver confident identification of low abundance proteins, unequivocal molecular weights and highest quality structural information. Robust new ion optics and a rugged detector help ensure long-term reliability and maximum up-time in demanding high-throughput environments.

3 The QSTAR XL Hybrid LC/MS/MS System is the highest performance quadrupole/time-of-flight (QqTOF) mass spectrometer available. Designed for demanding protein analysis, qualitative drug discovery and metabolomics studies, the system combines the superior mass selection of a quadrupole MS with the accuracy, resolution, and rapid full-scan capability of TOF technology. Unmatched sensitivity The combination of high-precision optics, advanced system design, innovative ionization sources, and powerful LINAC collision cell technology maximizes sensitivity across the full spectrum of proteomics and metabolomics applications. Expanded flexibility With fast, easy switching between the broadest selection of API and omaldi ion sources, the QSTAR XL System is well-suited to a wide range of proteomics and small molecule applications, making it a valuable and versatile addition to any discovery program. Powerful scan modes Novel scan functions let you extract more meaningful information from complex biological samples. The multiply charged scan feature offers a high degree of selectivity for low-level protein analysis; innovative precursor ion scanning capability enables accurate analysis of drug metabolites and post-translational modifications. intensity, counts intensity, counts + TOF MS: 124 MCA scans from Sample 6 (TOF 1:5 GS1=3 right CAD) of Dec2_BSA2fM_Nano.wiff a= e-4. t= e+ Max counts m/z, amu + TOF MS: 1262 MCA scans from Sample 8 (Nic's 12 All 11.5V) of Dec2_BSA2fM_Nano.wiff a= e-4. t= e Max. 61. counts m/z, amu In a typical nanospray TOF MS (top trace), the singly charged background obscures the multiply charged species at low femtomole concentration. In the bottom trace, the singly charged species are effectively removed, and the only peaks showing are the multiply charged species. Broader mass range Increased quadrupole mass selection of up to 6, m/z and increased time-of-flight mass range of up to 4, m/z, as well as enhanced collision induced dissociation (CID) capabilities and improved fragment ion transmission permit MS and MS/MS analysis of larger peptide and protein ions.

4 Unprecedented versatility. The QSTAR XL system generates superior quality MS and MS/MS data from both atmospheric pressure ionization (API) and matrix-assisted laser desorption ionization (MALDI) techniques. The unique ability to switch between MALDI and various API sources makes the system very well-suited for a wide range of discovery and development applications. intensity, counts m/z, amu MS/MS omaldi 2 Ion Source A novel orthogonal MALDI ion source delivers higher sensitivity and better control of front-end fragmentation. It uses collisional cooling to reduce the complexity of resulting mass spectra and enhance the stability of fragile peptide ions, enabling more thorough characterization of post-translationally modified proteins. Because of the orthogonal nature of the omaldi source, combined with the collisional cooling, accurate mass data can be collected even from complex and non-homogenous surfaces such as tissue slices. intensity, counts m/z, amu In addition to a conventional nitrogen laser, a new long-life, high repetition laser (Nd YAG) is now available. This high repetition laser offers: Smaller laser spot diameter for improved spatial resolution y(1) y(2), TSN DLL-2B POTAD y(5) NEEEN, THDEGN DNNPQ LLTQO, ONOLL GNASFQA y(6) EDNDL y(6) y(7) y(8) y(8) y(9) y(9) y(9) y(1) y(1) y(11) y(11) y(12) y(13) y(13) a(15), y(15) y(15), a(15) a(16), y(15) a(16), y(16) a(16) a(17), y(17) y(18) b(18) y(18) y(2) a(21), y(21) a(22) y(22) y(22) a(22), y(22) y(23), a(23) a(23), y(23) a(24), y(24) y(24) a(26), y(26) y(26) y(25) y(3) y(31) y(32) b(37) y(37) y(37) 1X faster acquisition time, especially beneficial for the LC MALDI workflow The increased mass range of the QSTAR XL system extends analysis capabilities, as depicted in this MS/MS identification of a 4179 Da peptide using automated acquisition and database searching software. Unambiguous identification of: DTSNNNNSNVNDEDNDLLTQDNNPQTADEGNASFQAQR

5 API Ion Sources NanoSpray and TurboIonSpray Ion Sources These rugged electrospray sources are optimized for sensitivity and reliability in protein and metabolite identification and characterization, and allow you to interface capillary and LC flow rates from 5 nl/min to 1 ml/min. PhotoSpray Photoionization Source The PhotoSpray photoionization source enables analysis of low molecular weight, highly polar compounds such as steroids and polycyclic aromatic hydrocarbons (PAHs) that are not easily detectable using conventional API techniques. APCI Source A versatile ion source for many drug discovery and development applications, the APCI source with heated nebulizer ionizes many compound classes in diverse solvent compositions across a broad range of flow rates. Novel MALDI imaging software used with the omaldi ion sources and omaldi Server 4. software allows users to acquire and process two-and three-dimensional images and generate contour plots of concentration distribution of low-molecular weight compounds in tissues. This can be an extremely powerful tool in studies to determine the distribution of drugs and metabolites in the body. MALDI image acquired directly from a mouse tumor tissue slice after treatment with a novel anti-tumor drug. This image shows distribution of the drug throughout the tumor. Data courtesy of M. Reyzer, Vanderbilt University.

6 Powerful software streamlines and automates your applications. Intuitive, application-specific software complements the exceptional performance of the QSTAR XL system. Information Dependent Acquisition (IDA) further extends automation capabilities, allowing you to automatically acquire MS and MS/MS data to derive maximum information from both ESI and MALDI experiments. Analyst QS software expertly handles core control and analysis functions, and works seamlessly with the following applications: Metabolite ID software simplifies and speeds up metabolite identification, rapidly sifting through large amounts of data and automatically giving you a selected list of potential metabolites. BioAnalyst software enables fast, automated processing of MS and MS/MS spectra for de novo sequencing, PTM analysis, intact protein analysis, and rapid identification of proteins with Pro ID. Pro QUANT software takes advantage of the spectral properties of itraq reagent labeled peptide MS/MS fragmentation to provide accurate, relative quantitation for your multiplexed samples. Pro ICAT software enables automated, high-throughput expression analysis of complex protein samples labeled with Applied Biosystems ICAT reagents. Pro Automate software provides intelligent integration of Pro ID, Pro ICAT, and Pro QUANT with Pro BLAST database searching capabilities for maximum protein information from known and unknown genomes. Xpert 2. software works with Analyst QS and omaldi server software to fully automate the acquisition, identification and quantitation of omaldi data, whether collected from unique sample spots or as an LC MALDI run. Xpert 2. software uses data processing algorithms and data acquisition methods optimized specifically for orthogonal MALDI analysis. Figure shows the distribution of Ketoconazole on the surface of porcine skin. Data was acquired after topical application of Nizarol antifungal shampoo in a triangular shape on the skin surface. Demonstrates ability of MALDI imaging technique to retain spatial information. Data courtesy of Josephine Bunch, Sheffield Hallam University. omaldi Server 4. allows the user to acquire omaldi ion sources data, including LC MALDI, using either manual or automatic acquisition with preset or custom laser search patterns. The optional MALDI Imaging Software gives users the ability to acquire mass spectrometric pictures of compounds on a MALDI plate.

7 The technology that makes superior performance possible Q Patented collisional focusing maximizes ion transmission for superior sensitivity. A proprietary dynamic Collisional Focusing technique plays a key role in achieving maximum sensitivity. It is used in the Q ion guide to focus ions into the quadrupole, and again in the high-pressure collision cell, where the ion beam is cooled and refocused into the TOF region. High-pressure Low-pressure DC Quad The quadrupole lens provides a marked improvement in the ability to optimize the ion beam profile. Detector An advanced four-anode detector provides extended dynamic range relative to conventional single channel detectors. A time-to-digital converter improves resolution and mass accuracy on low mass ions. Ultra Stable Quadrupole Mass Filter Sample Ion High Efficiencey LINAC Collision Cell TOF IQ2 Q2 IQ3-4kV Accelerator Slit Delay Extraction region V Trapping Releasing Q2 Patented LINAC high-pressure collision cell provides increased sensitivity and unique trapping/pulsing capability for maximum duty cycle. The patented LINAC collision cell with unique trapping/pulsing capabilities increases sensitivity of the QSTAR XL system for all scan types. This highly efficient storage-and-release mechanism provides a significant increase in duty cycle over all mass ranges.

8 Add performance and flexibility to your proteomics, drug discovery, or metabolomics program. If your research involves proteomics, drug discovery, or drug development studies, the Applied Biosystems/MDS SCIEX QSTAR XL Hybrid LC/MS/MS system can make your efforts more productive by giving you a sensitive, versatile system well-suited for a wide range of applications. For more information, call the Applied Biosystems sales office nearest you, or visit iscience. To better understand the complex interaction of biological processes, life scientists are developing revolutionary approaches to discovery that unite technology, informatics, and traditional laboratory research. In partnership with our customers, Applied Biosystems provides the innovative products, services, and knowledge resources that make this new, Integrated Science possible. Worldwide Sales Offices Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 15 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at Applera is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Headquarters 85 Lincoln Centre Drive Foster City, CA 9444 USA Phone: Toll Free: Fax: For Research Use Only. Not for use in diagnostic procedures. Applied Biosystems, Analyst, QSTAR, and TurboIonSpray are registered trademarks and AB (Design), iscience, iscience (Design), Applera, BioAnalyst, Interrogator, itraq, NanoSpray, and omaldi are trademarks of Applera Corporation or its subsidiaries in the US and certain other countries. PhotoSpray is a trademark of Applied Biosystems/ MDS SCIEX a joint venure between Applera Corporation and MDS Inc. LINAC, MDS, and SCIEX are registered trademarks of MDS Inc. ICAT is a registered trademark of the University of Washington, exclusively licensed to the Applied Biosystems Group of Applera Corporation. 24 Applied Biosystems. All rights reserved. Printed in the USA, 6/4, P+s, Publication 114BR13-1