Dr. Farrukh Sobia M.S., Ph.D.

Transcription

1 Dr. Farrukh Sobia M.S., Ph.D. Assistant Professor Microbiology, Department of Environmental Health Faculty of Public Health and Tropical Medicine Jazan University, Jizan Kingdom of Saudi Arabia Education Ph.D. (Doctor of Philosophy) in Medical Microbiology from J.N. Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh M. Sc. in Microbiology from Aligarh Muslim University, Aligarh, India B. Sc. (Hons.) in Botany from Aligarh Muslim University, Aligarh, India Senior Secondary School Certificate (10+2) with Physics, Chemistry, Biology (PCB) and English from Aligarh Muslim University, Aligarh, India Secondary School Certificate was obtained from Aligarh Muslim University, India. Research & Teaching Experience 2014-Present Assistant Professor in Microbiology, Faculty of Public Health and Tropical Medicine, Jazan, Jizan University, Kingdom of Saudi Arabia Indian University Grants Commission (UGC)-funded Dr. D.S Kothari post-doctoral fellowship (PDF) from J.N. Medical College & Hospital, Faculty of Medicine, Aligarh Muslim University, Aligarh papers published in International Journal, 4 Book chapters and 4 papers presented in Conferences and 2 Symposium & a workshop attended During Ph.D. Research Ten month training as Honorary research fellow in Molecular Biology Section, Department Of Microbiology, J.N. Medical College & Hospital, Aligarh Muslim University, Aligarh Six Month Training for M.Sc. Dissertation in Microbiology, Aligarh Muslim University, Aligarh Taught medical microbiology for MBBS second year during Ph.D. in the Microbiology Department, J.N. Medical College & Hospital, Aligarh Muslim University, Aligarh. Field of Interest Microbiology, Genetics and Molecular Biology, and bacterial Antimicrobial resistance. More specifically, mechanism and Resistance to beta-lactam antibiotics with special interest in AmpC-, CTX-M- and NDM-1-types β- lactamases, Molecular epidemiology of the resistance genes and mobilizing genetic elements (like Insertion sequences, integrons and plasmids)-mediated drug resistance. Identification and characterization of regions carrying resistance genes, and analyzing mechanism that leads to dissemination of the resistance genes. Awards & Achievements UGC, India awarded Dr. D. S. Kothari Post Doctoral Fellowship (Grant sanctioned for self written proposal). CSIR-NET (Council of scientific & Industrial Research-National Eligibility Test) qualified for Junior Research Fellowship in GATE (General Aptitude test for Engineering)-2007 (90 percentile).

2 Research Details Post Doctorate researcher (Sep Aug. 2013). Project topic of post-doc Research, Screening of clinical and environmental isolates of Enterobacteriaceae for class A (CTX-M, TEM, SHV) and class C (AmpC) β-lactamases, and NDMmetallo-beta-lactamases: exploring the load, reservoir, and genetic environment Find out the prevalence (and thus assessing the load) of bla CTX-M, bla ampc, and bla NDM-1 type genes in Indian bacterial population obtained from our hospitals. Find out the prevalence (and thus assessing the load) of bla CTX-M, bla ampc, and bla NDM-1 type genes in Indian bacterial population obtained from the environmental samples. Perform the epidemiological typing of the isolates so as to define any clonal relation or diversity. Perform sequencing of the CTX-M, AmpC, and NDM-1 genes in order to report any new gene or to detect the mutations in the existing genes. Doctorate researcher (Dec April 2012). Thesis topic of Ph.D. Graduation in Medical Microbiology, Occurrence, prevalence and genetic environment of Enterobacteriaceae with special reference to AmpC β-lactamases. Determine antibiotic resistance rates and patterns in cephamycin-resistant clinical E. coli and Klebsiella pneumoniae isolates from J. N. Medical College and Hospital, Aligarh, India. Screen the isolates for the presence of bla ampc genes and their characterization for the presence of respective AmpC-family. Analyze co-resistance of class A β-lactamase genes, especially the bla CTX-M. Explore plasmid and genetic elements [ISEcp1, IS26, ISCR1 (ORF513), Sul-1-type integron] responsible for mobilization of drug resistance genes in our isolates Honorary Research fellowship (Oct to Aug. 2008). Learned the molecular-biology techniques including PCR, Agarose gel Electrophoresis and plasmid DNA isolation. Rafi Ahmad Kidwai Institute of Agricultural Sciences, Aligarh Muslim University, Aligarh, Master s researcher (Aug to Sep. 2007). Dissertation topic of M.Sc. graduation in Microbiology, Plant growth promoting and bioremediation potential of rhizospheric bacteria isolated from metal contaminated soil ook Chapters: Published Research Articles in International Journals 1. M. Shahid, F. Sobia, A. Singh, A. Malik, H. M. Khan, D. Jonas, P. M. Hawkey Beta-lactams and beta-lactamase-inhibitors in Current or Potential-clinical Practice: A Comprehensive Update. Critical Reviews in Microbiology. 35(2): (Published by Informa Healthcare, U.S.A: Impact factor 5.01). 2. M. Shahid, F. Sobia, A. Singh, H. M. Khan, P.M. Hawkey, A. Huq, N. Khardori AmpC betalactamases and bacterial resistance: an updated mini-review. Reviews in Medical Microbiology. 20(3): M. Shahid, A. Shahzad, F. Sobia, A. Sahai, T. Tripathi, A. Singh, H. M. Khan and Umesh Plant natural Products as a potential source for antibacterial agents: Recent trends. Anti-infective Agents in Medicinal Chemistry. 8(3): (Published by Bentham Science Publications, U.S.A.)

3 4. M. Shahid, A. Shahzad, T. Tripathi, F. Sobia, A. Sahai, A. Singh, A. Malik, F. Shujatullah, H. M. Khan Recent Trends in Plant-Derived Antifungal Agents. Anti-infective Agents in Medicinal chemistry. 8(1): (Published by Bentham Science Publications, U.S.A.) 5. Mohammad Shahid, Trivendra Tripathi, Farrukh Sobia, Shagufta Moin, Mashiatullah Siddiqui, Rahat Ali Khan Histamine, Histamine Receptors, and their role in Immunomodulation: An Updated Systematic Review-The Open Immunology Journal. 2: (Published by Bentham Science Publications, U.S.A.). 6. Mohammad Shahid, Anuradha Singh, Farrukh Sobia, Mohammad Rashid, Abida Malik, Indu Shukla, Haris Manzoor Khan bla CTX-M, bla TEM, and bla SHV in Enterobacteriaceae from North-Indian tertiary care hospital: high occurrence of combination genes. Asia Pacific Journal of Tropical Medicine. 4(2): Mohammad Shahid, Anuradha Singh, Farrukh Sobia, Mitul Patel, Haris M Khan, Abida Malik, Indu Shukla An overview of CTX-M β-lactamases. Reviews in Medical Microbiology. 22(2): Farrukh Sobia, Mohammad Shahid, Anuradha Singh, Haris M. Khan, Indu Shukla, Abida Malik Occurrence of bla ampc in cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates from North- Indian tertiary care hospital. New Zealand Journal of Medical Laboratory Sciences. 65: Anuradha Singh, Mohammad Shahid, Farrukh Sobia, Haris M. Khan Comparative study on occurrence of class A and class C β-lactamase genes and their co-occurrence in Indian Enterobacteriaceae during years 2009 and Asia Pacific Journal of Tropical Medicine Anuradha Singh, Mohammad Shahid, Farrukh Sobia, Umesh, Haris M. Khan Occurrence and molecular epidemiology of bla CTX-M, including co-occurrence of bla TEM and bla SHV genes, and sul-1- association in Indian Enterobacteriaceae. International Journal of Antimicrobial Agents. 39: 184. (Impact factor: 3.4) 11. Mohd. Shahid, Farrukh Sobia, Anuradha Singh, Haris M Khan Concurrent occurrence of bla ampcfamilies and bla CTX-M-genogroups and association with mobile genetic elements ISEcp1, IS26, ISCR1, & Sul-1-type class1 integrons in Escherichia coli and Klebsiella pneumonia isolates originating from India. Journal of Clinical Microbiology. 50(5): (Impact Factor: 5.0) 12. Mohammad Shahid, Umesh, Farrukh Sobia, Anuradha Singh, Haris M. Khan, Abida Malik and Indu Shukla Molecular epidemiology of carbapenem-resistant Enterobacteriaceae from a North Indian tertiary hospital. New Zealand Journal of Medical Laboratory Sciences. 66: Mohd Shahid, Haris M Khan, Farrukh Sobia, Anuradha Singh, Fatima Khan, M Salman Shah, Indu Shukla, Abida Malik, Iqbal M. Khan Molecular epidemiology of various antibiotic resistance genes, including bla NDM-1, in Indian environmental and clinical bacterial isolates: a comparative study. New Zealand Journal of Medical Laboratory Sciences. 67: Farrukh Sobia, MoinUddin, M. Shahid, Haris M. Khan Characterization of Escherichia coli and Klebsiella pneumonia isolates for bla ESBLs, bla ampc and bla NDM-1 isolates from a north-indian tertiary hospital: a preliminary time trend study. New Zealand Journal of Medical Laboratory Sciences. 68:

4 Book Chapters 1. M. Shahid, T. Tripathi, F. Sobia, A. Singh, A. Malik, and H.M. Khan Potential Biological Effects of Drugs and Pharmaceuticals on Environment Health System. In: An Introduction to Environmental Pharmacology (ISBN # ), Eds. SZ Rahman, M. Shahid & V Gupta. Ibn Sina Academy, Aligarh, India; 2008, pp Trivendra Tripathi, Mohd. Shahid, Rahat Ali Khan, Haris M. Khan, Farrukh Sobia, Anuradha Singh, Mashiatullah Siddiqui Histamine: Role in the pathogenesis of autoimmune, Allergic, and malignant diseases. In: Biomedical Aspects of Histamine: New Perspectives, Eds. Shahid M, Khardori N, Khan RA, Tripathi T (Eds). Springer-Verlag GmbH Heidelberg, Germany (ISBN: ). 3. Trivendra Tripathi, Mohd. Shahid, Farrukh Sobia, Anuradha Singh, Haris M. Khan, Rahat Ali Khan, Mashiatullah Siddiqui Immune regulation by various facets of histamine in immunomodulation and allergic disorders. In: Biomedical Aspects of Histamine: New Perspectives, Eds. Shahid M, Khardori N, Khan RA, Tripathi T (Eds). Springer-Verlag GmbH Heidelberg, Germany (ISBN: ); pp Mohd. Shahid, Hridesh Mishra, Hemant Kumar Mishra, Trivendra Tripathi, Haris M. Khan, Farrukh Sobia, Anuradha Singh Pharmaco-EcoMicrobiology and its potential role in medical and environmental sciences. In: Research on Pharmacoinformatics: Technologies, Theories and Applications ; pp Tagelsir Gamelseid (Editor), IGI Global, USA. Paper Presented / Abstract in Conference F. Sobia, M. Shahid, Haris M Khan, A. Singh Prevalence of bla ampc genes in clinical isolates recovered from a tertiary care hospital. In: 6 th Annual Conference of Association of Medical Microbiologists-UP Chapter U.P. MICROCON 2010 & CME on Newer Molecular tool for Diagnosis & Management of tuberculosis & other Mycobacterial diseases, Organized by National JALMA Institute for Leprosy & other Mycobacterial Diseases (ICMR), on 13 th -14 th March, A. Singh, M. Shahid, Haris M Khan, F. Sobia Prevalence of CTX-M genogroups in E. coli and Klebsiella isolates from a North Indian Tertiary Hospital. In: 6 th Annual Conference of Association of Medical Microbiologists-UP Chapter U.P. MICROCON 2010 & CME on Newer Molecular tool for Diagnosis & Management of tuberculosis & other Mycobacterial diseases, Organized by National JALMA Institute for Leprosy & other Mycobacterial Diseases (ICMR), on 13 th - 14 th March, Farrukh Sobia, Haris M Khan, M. Shahid, Anuradha Singh, Abida Malik, Indu Shukla Receptor-Ligand interaction of Plasmodium vivax and Duffy blood group: a Literature review. In a national symposium on Malaria-an Update organized by Department of Microbiology, J. N. Medical College, Aligarh Muslim University, on 18 th December, A. Singh, M. Shahid, Haris M Khan, F. Sobia Polymorphic antigen markers and genetic diversity of Plasmodium vivax: a literature review. In: a national symposium on Malaria-an Update organized by Department of Microbiology, J. N. Medical College, Aligarh Muslim University, on 18 th December, 2010.

5 Sequences Published in GenBank DNA Sequence Escherichia coli AmpC beta-lactamase-like (bla CMY-2) gene, partial sequence Escherichia coli AmpC beta-lactamase (bla EBC) gene, partial cds Escherichia coli class I integron dihydrofolate reductase (dfra17) and aminoglycoside adenylyltransferase (aada5) genes, partial cds Escherichia coli insertion sequence ISEcp1, partial sequence; and insertion sequence IS26 cefotaximase (bla CTX-M-15) gene, partial cds Accession Number JF JF JF JF Symposium and Workshop attended A two-day bioinformatics workshop on Exploring Proteins with Bioinformatics, organized by Distribution Information sub-centre, Biotechnology Building, Aligarh Muslim University, Aligarh, from January, A one-day symposium on Emerging trends in dengue and other hemorrhagic fevers in India organized by Department of Microbiology, J. N. M.C., A.M.U, Aligarh, on 8 th October, Techniques Acquainted with Routine microbiological techniques including bacterial culture, Biochemical characterization of microorganism and antimicrobial susceptibility. Advanced molecular techniques including PCR, Plasmid analysis, RAPD typing, restriction analysis, bacterial conjugation and Agarose gel electrophoresis. Routine Immunological techniques including Haemagglutination, ELISA. Awareness of sequencing and alignment software as well as related websites including Blast, Clustal W, GenBank. Computer Skills Operating System: MS- Windows, Linux General Packages: MS-Office (MS-Word, MS-Excel, & MS-Power point) Others: Internet Surfing and other basic work. Reference Dr. Mohd. Shahid Prof. Haris M. Khan Dr. Moinuddin M.B.B.S., M.D., Ph.D., FNZIMLS M.B.B.S., M.D. M.S., Ph.D. Associate Professor Professor Associate Professor Department of Microbiology Department of Microbiology Department of Biochemistry J. N. Medical College & Hospital J. N. Medical College & Hospital J. N. Medical College & Hospital A.M.U., Aligarh, (INDIA) A.M.U., Aligarh, (INDIA) A.M.U., Aligarh, (INDIA) ; shahidsahar@yahoo.co.in ;harismk2003@hotmail.com ;moin_u@rediffmail.com I, hereby declare that all the above information is true to the best of my knowledge and belief. Farrukh Sobia