Novel Sensor Platforms for Food Safety 2 Dec Dr Meng Han Kuok Camtech Innovations Ltd

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1 Novel Sensor Platforms for Food Safety 2 Dec 2015 Dr Meng Han Kuok Camtech Innovations Ltd

2 Beef Faecal Detector Novel sensing technology applicable to bacterial detection in a broad range of food testing applications

3 Detec%on of faecal contamina%on spots on meat: Real- Time and Non- Contact ~$1 billion/yr of spoiled beef in US alone

4 Faecal contamina%on detec%on on meat: Real- Time and Non- Contact primary vector of bacteria contamination! Zero Faecal Tolerance Policy in US and China

5 Feature Feature PREVENTION Comparison Camtech Diagnos8cs Camtech Diagnos8cs Visual inspec8on Low false posi%ve and nega%ve Yes No No Detect non- visible contamina%on Yes No No Lab culture Portable tester Real- %me speed Yes No (days) No (min- hrs) Non- contact with sample Yes (~500mm) No (swab) No (swab) Sample prepara%on not required Yes No No Consumables not required Yes No No Portable Yes No Yes User- friendly Yes No No Easy to clean Yes No No Washing Stop contamina%on at point Yes No No (spreading) Cost effec%ve Yes No No DIAGNOSTIC Comparison

6 Increase shelf- life ZERO faeces Benefits Product confidence Lean produc8on Value Brand protec8on Proper audi8ng

7 Product launch 2015

8 TECHNOLOGY 1. R&D Collabora%on to expand the technology for other diagnos%c applica%ons LOOKING AHEAD OPPORTUNITIES PRODUCT 1. Sales & marke%ng partnerships 2. Collabora%on with other technologies to achieve add- on func%ons 3. Next genera%on detector: Automated In- Line Scanner

9 Making Point of Need Testing a reality Delivering innovative lab-on-a-chip solutions Faster, cheaper point of need testing 9

10 Microfluidic biosensors Advantages Simple integration of functions on a single chip Miniaturisation of test for handheld devices Real time, multiplexed testing Low cost, smaller volumes of reagent required High sensitivity Detection or monitoring of viable cells Alternative to PCR and plate count methods 10

11 Handheld lab-on-chip biosensor Op%cal Handheld Reader Disposable diagnos%c chip 11

12 Optical Smart Polymer Sensor hydrogel ligand d Smart polymer white light! R 1 R 2 R 3 R 4 R total = f! i= 1 R f R i R = reflected light ultrafine silver grains (~20nm diameter) smart polymeric matrix film 12

13 white light blue light white light Color change readable by eye green light white light red light Contracted hologram interact with analyte 1 Contraction HOLOGRAM Expansion interact with analyte 2 Expanded hologram Intensity Wavelength (nm)

14 Camtech Diagnostic Platform label-free customisable Lab-on-a-chip platform: Miniaturisation: portable, perfect for first responders Speed: results within 15min Multiplex: detection of a range of biological agents and toxins Automated: user-friendly, just click the button No contamination: disposable chip Customised Sensing real-time optically readable 14

15 Key benefits of Lab on Chip solution Rapid results within 15 minutes Portable perfect for point of need testing User friendly no reagents or complex sample prep Durable ideal for field use Multiplex detection of a range of target analytes 15

16 Sensor Response Wavelength blue- shiz associated with germina%on and outgrowth of spores in nutrient broth (95nM, Sigma 70112) and D- glucose (5mM, Sigma G8270) 16

17 Conclusion Hologram sensor film was successfully integrated into a multilayer PMMA microfluidic chip A UV-spectrophotometer integrated fluid manipulation system was developed to facilitate the microfluidic based spore detection A 6nm blue shift in wavelength was detected in 15mins during germination The system is promising as a portable point of use device for fast, label free detection of the presence of bacterial spores Sensor microfluidic chip and fluid manipulation system offers a platform for sample prep and test in diagnostic applications 17

18 THANK YOU Dr Meng- Han Kuok