Material Safety Data Sheet

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1 Material Safety Data Sheet NFPA HMIS Personal Protective Equipment 1 Health Hazard Fire Hazard 1 See Section 15. Section 1. Chemical Product and Company Identification Common Name/ Trade Name Manufacturer Commercial Name(s) Synonym Chemical Name DNA Gel Loading Buffer, 6X SPECTRUM LABORATORY PRODUCTS INC S. SAN PEDRO STREET GARDENA, CA 9248 Catalog Number(s). CAS# RTECS TSCA CI# D378 Mixture. TSCA 8(b) inventory: Water; Amaranth; Patent blue; Bromophenol blue; Ficoll IN CASE OF EMERGENCY CHEMTREC (24hr) Page Number: 1 Chemical Family Chemical Formula Supplier SPECTRUM LABORATORY PRODUCTS INC S. SAN PEDRO STREET GARDENA, CA 9248 CALL (31) Section 2.Composition and Information on Ingredients Name CAS # Exposure Limits TWA (mg/m 3 ) STEL (mg/m 3 ) CEIL (mg/m 3 ) % by Weight 1) Water ) Amaranth ) Ficoll ) Patent blue <5. 5) Bromophenol blue <5. Toxicological Data on Ingredients Amaranth: ORAL (LD5): Patent blue: ORAL (LD5): Acute: 6 mg/kg [Rat]. >1 mg/kg [Mouse]. Acute: 3 mg/kg [Rat]. Section 3. Hazards Identification Potential Acute Health Effects Potential Chronic Health Effects Slightly hazardous in case of skin contact (irritant, permeator), of eye contact (irritant), of ingestion, inhalation. CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Amaranth]. MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast. [Amaranth]. Mutagenic for bacteria and/or yeast. [Patent blue]. TERATOGENIC EFFECTS: DEVELOPMENTAL TOXICITY: The substance is toxic to mucous membranes. Repeated or prolonged exposure to the substance can produce target organs damage.

2 DNA Gel Loading Buffer, 6X Page Number: 2 Section 4. First Aid Measures Eye Contact Skin Contact Serious Skin Contact Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention. In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove contaminated clothing and shoes. Cold water may be used.wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. Inhalation Serious Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. Ingestion Serious Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms appear. Section 5. Fire and Explosion Data Flammability of the Product Auto-Ignition Temperature Flash Points Flammable Limits Products of Combustion Fire Hazards in Presence of Various Substances Explosion Hazards in Presence of Various Substances Fire Fighting Media and Instructions Fire Hazards Explosion Hazards Non-flammable. Not available Not available Section 6. Accidental Release Measures Small Spill Large Spill Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority requirements. Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system.

3 DNA Gel Loading Buffer, 6X Page Number: 3 Section 7. Handling and Storage Precautions Storage Do not ingest. Do not breathe gas/fumes/ vapor/spray. Wear suitable protective clothing. If ingested, seek medical advice immediately and show the container or the label. Keep away from incompatibles such as oxidizing agents. Keep container tightly closed. Keep container in a cool, well-ventilated area. Section 8. Exposure Controls/Personal Protection Engineering Controls Personal Protection Personal Protection in Case of a Large Spill Exposure Limits Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value. Safety glasses. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self contained breathing apparatus should be used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product. Section 9. Physical and Chemical Properties Physical state and appearance Liquid. (Viscous liquid.) Odor Molecular Weight ph (1% soln/water) Not available Taste Color Purple. (Dark.) Boiling Point The lowest known value is 1 C (212 F) (Water). Melting Point Critical Temperature Specific Gravity Weighted average: 1.2 (Water = 1) Vapor Pressure Vapor Density Volatility Odor Threshold Water/Oil Dist. Coeff. Ionicity (in Water) Dispersion Properties Solubility The highest known value is 2.3 kpa (@ 2 C) (Water). The highest known value is.62 (Air = 1) (Water). See solubility in water. Soluble in cold water, hot water. Section 1. Stability and Data Stability Instability Temperature Conditions of Instability Incompatibility with various substances Corrosivity The product is stable. Incompatible materials Reactive with oxidizing agents. Slightly reactive to reactive with reducing agents. Non-corrosive in presence of glass. Incompatible with strong oxiders. (Bromophenol blue)

4 DNA Gel Loading Buffer, 6X Page Number: 4 Corrosivity Polymerization Will not occur. Section 11. Toxicological Information Routes of Entry Toxicity to Animals Chronic Effects on Humans Other Toxic Effects on Humans Toxicity to Animals Chronic Effects on Humans other Toxic Effects on Humans Absorbed through skin. Eye contact. Ingestion. Acute oral toxicity (LD5): 3 mg/kg [Rat]. (Patent blue). CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Amaranth]. MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast. [Amaranth]. Mutagenic for bacteria and/or yeast. [Patent blue]. Hazardous in case of inhalation (lung irritant). Slightly hazardous in case of skin contact (irritant, permeator), of ingestion,. May affect genetic material (mutagenic). May cause cancer based on animal test data. No adequate human data. May cause adverse reproductive effects and birth defects (teratogenic). (Amaranth) May affect genetic material (mutagenic). (Patent Blue) Acute Potential Health Effects: Skin: May cause skin irritation. It may stain the skin Eyes: May cause eye irritation. Inhalation: Dust may cause respiratory tract irritation. Ingestion: May cause gastrointestinal tract irritation. Expected to be a low hazard. Section 12. Ecological Information Ecotoxicity BOD5 and COD Products of Biodegradation Toxicity of the Products of Biodegradation the Products of Biodegradation Possibly hazardous short term degradation products are not likely. However, long term degradation products may arise. The products of degradation are more toxic than the product itself. Section 13. Disposal Considerations Waste Disposal Waste must be disposed of in accordance with federal, state and local environmental control regulations. Section 14. Transport Information DOT Classification Identification Not a DOT controlled material (United States). Special Provisions for Transport DOT

5 DNA Gel Loading Buffer, 6X Page Number: 5 Section 15. Other Regulatory Information and Pictograms Federal and State Regulations California Proposition 65 Warnings Other Regulations TSCA 8(b) inventory: Water; Amaranth; Patent blue; Bromophenol blue; Ficoll California prop. 65: This product contains the following ingredients for which the State of California has found to cause cancer which would require a warning under the statute: No products were found. California prop. 65: This product contains the following ingredients for which the State of California has found to cause birth defects which would require a warning under the statute: No products were found. OSHA: Hazardous by definition of Hazard Communication Standard (29 CFR ). Other Classifications WHMIS (Canada) Not controlled under WHMIS (Canada). DSCL (EEC) This product is not classified according to the EU regulations. Not applicable HMIS (U.S.A.) Health Hazard Fire Hazard Personal Protection 1 g National Fire Protection Association (U.S.A.) Health 1 Flammability Specific hazard WHMIS (Canada) DSCL (Europe) TDG (Canada) ADR (Europe) Protective Equipment Gloves. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Safety glasses.

6 DNA Gel Loading Buffer, 6X Page Number: 6 Section 16. Other Information MSDS Code References Other Special Considerations D3595 Validated by Sonia Owen on 2/18/211. Verified by Sonia Owen. Printed 2/18/211. CALL (31) Notice to Reader All chemicals may pose unknown hazards and should be used with caution. This Material Safety Data Sheet (MSDS) applies only to the material as packaged. If this product is combined with other materials, deteriorates, or becomes contaminated, it may pose hazards not mentioned in this MSDS. It shall be the user's responsibility to develop proper methods of handling and personal protection based on the actual conditions of use. While this MSDS is based on technical data judged to be reliable, Spectrum Quality Products, Inc. assumes no responsibility for the completeness or accuracy of the information contained herein.