enix Stem Cell Toxicity Assays

Transcription

1 HemoGenix enix Stem Cell Toxicity Assays The Bioluminomics TM Principle Assay products for high throughput in vitro toxicity screening during early and late stage drug development. The assay products have been designed for: The assays are designed for: Basic research toxicology and pharmacology Toxicology, risk and safety assessment for biopharmaceutical companies Environmental testing HemoGenix provides assays for in vitro toxicity screening. The testing principle is based on the determination of the intracellular ATP-concentration by measuring the correlated bioluminescence on 96-well plates. For validation the assay kits include standard & controls. The Bioluminomics TM Principle: Luciferase ATP + Luciferin + O₂ > Oxyluciferin + AMP + PPi + CO₂ ₂ + LIGHT Mg 2+

2 HemoGenix Toxicity Assays are available for various cell types: CELL TYPE HEMATOPIETIC STEM CELLS MESENCHYMAL STROMA CELLS ASSAY NAME HALO -Tox HT PRT MSCGlo -Tox HT ASSAY TYPE Suspension Expansion Culture TM Adherent cell expansion APPLICATION High throughput hemotoxicity screening Residual hemotoxicity and drug sensitivity change High throughput MSC cytotoxicity screening IMMUNE CELLS ImmunoGlo -Tox HT High throughput toxicity screening PRIMARY STEM CELLS AND CELL LINES NEURAL STEM CELLS STEMGlo -Tox Tox HT STEMGlo -PRT NeuroGlo -Tox HT Adherence or non-adherence growth-expansion High throughput stem cell toxicity screening Residual stem cell toxicity and drug sensitivity change High throughput neurotoxicity screening HEPATOCYTES HepatoGlo -Tox HT High throughput hepatoxicity screening Readout: ATP bioluminescence (96- or 384-well plate) Kit Components: Master Mix, ATP-Standard, ATP-Controls, 96-wll Plate(s), Sterile adhesive foil, Manual Toxicity Testing for Hematopoietic Stem and Progenitor Cells Tox ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls K2-0-2HH Tox ATP bioluminescence kit 2 Plate Kit K2-HPP2-2HH Tox ATP bioluminescence kit for HPP-SP2 2 Plate Kit K2-GEMM1-2HH Tox ATP bioluminescence kit for CFC-GEMM1 2 Plate Kit K2-BFU1-2HH Tox ATP bioluminescence kit for BFU-E1 2 Plate Kit K2-BFU2-2HH Tox ATP bioluminescence kit for BFU-E2 2 Plate Kit K2-GM1-2HH Tox ATP bioluminescence kit for GM-CFC1 2 Plate Kit K2-GM3-2HH Tox ATP bioluminescence kit for GM-CFC3 2 Plate Kit K2-MK1-2HH Tox ATP bioluminescence kit for Mk-CFC1 2 Plate Kit K2-T-2HH Tox ATP bioluminescence kit for T-CFC 2 Plate Kit K2-B-2HH Tox ATP bioluminescence kit for B-CFC 2 Plate Kit

3 PRT Predictive Residual Hemotoxicity Assay When cells respond to cytotoxic agents, not all the cells may be inhibited or killed. The cells that are not affected are residual cells. When removed and re-plated, these cells may still be capable of proliferation. The residual dose response after secondary re-plating may indicate a shift to the left or right indicating a change in agent/drug sensitivity. Secondary re-plating also indicate the presence of stem cells from that tissue or organ. K2-PRT-2H Tox ATP biolum. kit HPP-SP1 + HPP-SP2 Human 2 Plate Kit K2-PRT-2PR Tox ATP biolum. kit HPP-SP1 + HPP-SP2 Primate 2 Plate Kit K2-PRT-2C Tox ATP biolum. kit HPP-SP1 + HPP-SP2 Dog 2 Plate Kit K2-PRT-2R Tox ATP biolum. kit HPP-SP1 + HPP-SP2 Rat 2 Plate Kit K2-BFU2-2M Tox ATP biolum. kit HPP-SP1 + HPP-SP2 Mouse 2 Plate Kit Tox 4 Population Kit 4 Population Lympho-Hematoxicity Assay (Control +CFC-GEMM1, +BFU-E1, +GM-CFC1 + Mk-CFC1) K2-4P-5HH Tox 4 Population Assay Human 5 Plate Kit K2-4P-5PRH Tox 4 Population Assay NHP 5 Plate Kit K2-4P-5CH Tox 4 Population Assay Dog 5 Plate Kit K2-4P-5RH Tox 4 Population Assay Rat 5 Plate Kit K2-4P-5MH Tox 4 Population Assay Mouse 5 Plate Kit Tox 5 Population Kit 5 Population Lympho-Hematoxicity Assay (Control + HPP-SP2, +CFC-GEMM1, +BFU-E1, +GM-CFC1 + Mk-CFC1) K2-5P-6HH Tox 5 Population Assay Human 6 Plate Kit K2-5P-6PRH Tox 5 Population Assay NHP 6 Plate Kit K2-5P-6CH Tox 5 Population Assay Dog 6 Plate Kit K2-5P-6RH Tox 5 Population Assay Rat 6 Plate Kit K2-5P-6MH Tox 5 Population Assay Mouse 6 Plate Kit Tox 7 Population Kit 7 Population Lympho-Hematox Assay (BC + HPP-SP2, +CFC-GEMM1, +BFU-E1, +GM-CFC1 + MK-CFC1+T-CFC + B-CFC) K2-7-8HH Tox 7 Population Assay Human 8 Plate Kit K2-7P-8PRH Tox 7 Population Assay NHP 8 Plate Kit

4 Tox 7 Population Assay Dog 8 Plate Kit K2-7P-8RH Tox 7 Population Assay Rat 8 Plate Kit K2-7P-8MH Tox 7 Population Assay Mouse 8 Plate Kit CAMEO -4 A miniaturized methylcellulose CFC assay used in toxicity testing to detect the response of compounds on the differentiation and maturation process Easy handing: due to the optimized blend of the master mix it can simply be applied with a poistive displacement pipette Fast results in days: media formulations allows increased growth of colonies Economical use of cells: the miniaturized CFU-assay kit CAMEO TM -4 requires only 1/10 the amount of target cells compared to traditional assays KC-0-50H CAMEO TM -4 without Growth Factors KC-HPP1-50H CAMEO TM -4 HPP-SP1 KC-HPP2-50H CAMEO TM -4 HPP-SP2 KC-GEMM1-50H CAMEO TM -4 for CFC-GEMM1 KC-GEMM2-50H CAMEO TM -4 for CFC-GEMM2 KC-GEMM3-50H CAMEO TM -4 for CFC-GEMM3 KC-GEM1-50H CAMEO TM -4 for CFC-GEM1 KC-GEM2-50H CAMEO TM -4 for CFC-GEM2 KC-GEM3-50H CAMEO TM -4 for CFC-GEM3 KC-BFU1-50H CAMEO TM -4 for BFU-E1 KC-BFU2-50H CAMEO TM -4 for BFU-E2 KC-E-50H CAMEO TM -4 for CFU-E KC-GM1-50H CAMEO TM -4 for GM-CFC1 KC-GM2-50H CAMEO TM -4 for GM-CFC2 KC-GM3-50H CAMEO TM -4 for GM-CFC3 KC-G-50H CAMEO TM -4 for G- CFC KC-M-50H CAMEO TM -4 for M-CFC KC-MK1-50H CAMEO TM -4 for Mk-CFC1 KC-MK2-50H CAMEO TM -4 for Mk-CFC2 KC-GM1-50H CAMEO TM -4 for GM-CFC1 KC-GM2-50H CAMEO TM -4 for T-CFC KC-GM3-50H CAMEO TM -4 for B-CFC

5 IN VITRO Immunotoxicity Testing Although often considered a separate area, immunotoxicity and hemotoxicity are intimately related since the immune and hematopoietic systems share common primitive stem cells. For more mature immune cell populations ImmunoGlo -Tox HT Assay for in vitro Immunotoxicity This 384-well plate in vitro immunotoxicity testing system can be used to detect cytotoxicity associated with stimulator and responder cells. KM1-T384-4 ImmunoGlo TM -Tox HAT for user defined immune cells 4 x 384-plate IN VITRO Mesenchymal Stem Cell Toxicity Toxicity Testing The production of osteoblasts, adipocytes and chondrocytes play a large role in regenerative medicine. These cell types are all derived from mesenchymal stem cells (MSCs). Mesenchymal stem cells are also part of the hematopoietic stroma of the hematopoietic stem cell niche. MSCGlo -Tox HT Assays for in vitro MSC Toxicity MSC bioluminescence toxicity platform utilizing Bioluminomics technology available for following species: Human, NHP, Rat, Mouse KLMC-T96H-2H MSCGlo TM -HT with Humanized Media 2 x 96-well plate KLMC-T96H-4H MSCGlo TM -HT with Humanized Media 4 x 96-well plate KLMC-T96LS-2H MSCGlo TM -HT with Low serum Media 2 x 96-well plate KLMC-T96LS-4H MSCGlo TM -HT with Low serum Media 4 x 96-well plate KLMC-T96SF-2H MSCGlo TM -HT with Serum Free Media 2 x 96-well plate KLMC-T96SF-4H MSCGlo TM -HT with Serum Free Media 4 x 96-well plate

6 MSCGlo -96 PRT MSC predictive residual toxicity assay When cells respond to cytotoxic agents, not all the cells may be inhibited or killed. The cells that are not affected are residual cells. When removed and re-plated, these cells may still be capable of proliferation. The residual dose response after secondary re-plating may indicate a shift to the left or right indicating a change in agent/drug sensitivity. Secondary re-plating also indicate the presence of stem cells from that tissue or organ. KLMC-PRTLS-2 MSCGlo TM -96 PRT with low serum media 2 x 96-well plate KLMC-PRTSF-2 MSCGlo TM -96 PRT with serum free media 2 x 96-well plate IN VITRO Toxicity Testing for Primary Cells, ES and ips Cells STEMGlo -assays with Bioluminomics technology are suitable to measure toxicity on various cell types: Hepatotoxicity Neurotoxicity Renal toxicity Cardiotoxicity Skin toxicity Lung toxicity STEMGlo -Tox Tox HT Assay to measure potential toxicity using different cell types from a variety of mammalian species KSG-T96-A-2 StemGlo TM -Tox HT for adherent cells 2 x 96-well plate KSG-T96-A-4 StemGlo TM -Tox HT for adherent cells 4 x 96-well plate KSG-T96-NA-2 StemGlo TM -Tox HT for non-adherent cells 2 x 96-well plate KSG-T96-NA-4 StemGlo TM -Tox HT for non-adherent cells 4 x 96-well plate KSG-T384-A-2 StemGlo TM -Tox HT for non-adherent cells 2 x 384-well plate KSG-T384-A-2 StemGlo TM -Tox HT for non-adherent cells 4 x 384-well plate KSG-T384-NA-2 StemGloTM-Tox HT for non-adherent cells 2 x 384-well plate KSG-T384-NA-2 StemGloTM-Tox HT for non-adherent cells 4 x 384-well plate

7 STEMGlo -PRT Predictive Residual Toxicity Assay When cells respond to cytotoxic agents, not all the cells may be inhibited or killed. The cells that are not affected are residual cells. When removed and re-plated, these cells may still be capable of proliferation. The residual dose response after secondary re-plating may indicate a shift to the left or right indicating a change in agent/drug sensitivity. Secondary re-plating also indicate the presence of stem cells from that tissue or organ. KSG-PRT-A-2 StemGlo TM -PRT for adherent cells 2 x 96-well plate KSG-PRT-NA-2 StemGlo TM -PRT for non-adherent cells 2 x 96-well plate HepatoGlo -Tox HT Hepatotoxicity ATP-bioluminescent assay for incorporation of fresh or frozen primary or ips-derived hepatocytes HepatoGlo TM specialized growth medium KHG-96-A-2 HepatoGlo TM -Tox HT for adherent cells 2 x 96-well plate kit KHG-96-NA-2 HepatoGlo TM -Tox HT for non-adherent cells 2 x 96-well plate kit NeuroGlo -Tox HT ATP-bioluminescent assay for primary - or ips-derived neuronal cells with specialized growth medium (on request) STEMClone Clonal Methylcellulose Proliferation and Differentiation Assay Manual Colony Enumeration AND Standardized ATP Bioluminescence Based on the classic colony-forming cell (CFC)-assay KHG-96-A-1 STEMClone TM for primary stem cells and stem cell lines 1 x 96-well plate kit KHG-96-A-2 STEMClone TM for primary stem cells and stem cell lines 2 x 96-well plate kit KHG-96-A-4 STEMClone TM for primary stem cells and stem cell lines 4 x 96-well plate kit White Paper "Stem Cells and Predictive In Vitro Toxicity Testing"